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51 - 100 of 2606 citations for Recombinant Human IL6ST Fc His tagged since 2019

• Enhancing extracellular vesicle cargo loading and functional delivery by engineering protein-lipid interactions

  Justin A. Peruzzi, et al., bioRxiv - Bioengineering 2023

  Quote: ... purified FLAG tagged protein (recombinant p53 protein, R&D Systems) was run with vesicles to quantify loading ...
• Antibody-lectin chimeras for glyco-immune checkpoint blockade

  Jessica C. Stark, et al., bioRxiv - Bioengineering 2024

  Quote: ... Siglec-Fc or human IgG1 isotype Fc (R&D Systems) precomplexes with Alexa Fluor 647 (AF647)-labeled donkey anti-human IgG antibody (Jackson ImmunoResearch ...
• The fiber knob protein of human adenovirus type 49 mediates highly efficient and promiscuous infection of cancer cell lines using a novel cell entry mechanism

  Alexander T. Baker, et al., bioRxiv - Microbiology 2020

  Quote: ... Approximately 5000 RU of Recombinant Human Desmoglein-2 Fc Chimera Protein (R&D Systems, Catalogue number 947-DM-100) was amine coupled to a CM5 sensor chip at a slow flow-rate of 10 μl/min to ensure uniform distribution on the chip surface.
• Lentiviral vector optimization enhances the expression and cytotoxicity of chimeric antigen receptors

  Changjiang Guo, et al., bioRxiv - Cell Biology 2021

  Quote: ... NKG2DLs expression in each cancer cell line was stained with recombinant human NKG2D Fc chimera (R&D Systems, USA) and then FITC-conjugated anti-human Fc secondary antibody was used as a detection antibody (BD Biosciences ...
• P66 is a bacterial mimic of CD47 that binds the anti-phagocytic receptor SIRPα and facilitates macrophage evasion by Borrelia burgdorferi

  Michal Caspi Tal, et al., bioRxiv - Immunology 2024

  Quote: ... were suspended in 200 μL of a 125 nM solution of recombinant human SIRPα-Fc protein (R&D Systems), the cognate isotype control recombinant human IgG1 protein (Sino Biological) ...
• An unconventional cerebrospinal fluid-derived Semaphorin-signalling regulates apical progenitor dynamics in the developing neocortex

  Katrin Gerstmann, et al., bioRxiv - Neuroscience 2020

  Quote: ... we incubated brain hemispheres with recombinant Sema3B-Alkaline Phosphatase in the presence or absence of recombinant Nrp1-Fc or Nrp2-Fc (R&D systems), respectively (10 ng/µl in PBS) ...
• DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells

  Yale S. Michaels, et al., bioRxiv - Bioengineering 2021

  Quote: ... and 2.5ug/mL Fc-tagged VCAM1 (R&D Systems, 643-VM). Tissue culture-treated 96 well plates (Fisher Scientific ...
• Time- and lineage-resolved transcriptional profiling uncovers gene expression programs and clonal relationships that underlie human T lineage specification

  Yale S. Michaels, et al., bioRxiv - Immunology 2023

  Quote: ... Fc-tagged VCAM1 (2.5 μg/ml; R&D Systems, 643-VM) and Fc-tagged DLL4 (15 μg/ml ...
• The GPI sidechain of Toxoplasma gondii prevents parasite pathogenesis

  Julia A Alvarez, et al., bioRxiv - Microbiology 2024

  Quote: ... or recombinant CD36-Fc (R&D Systems, #2519-CD) in 50μl binding buffer (0.14 M NaCl ...
• Non-glycosylated IGF2 prohormones are more mitogenic than native IGF2

  Pavlo Potalitsyn, et al., bioRxiv - Biochemistry 2023

  Quote: ... His-tagged ALS protein (R&D Systems, 10 ng/well, 1.5 µM) was immobilized in wells via neutravidin and the nickel-charged-NTA-decorated iBodies® (IOCB Tech ...
• Alveolar cell fate selection and lifelong maintenance of AT2 cells by FGF signaling

  Douglas G. Brownfield, et al., bioRxiv - Developmental Biology 2022

  Quote: ... Fgfr2iiib-Fc (human, R&D Systems) and Phospho-p44/42 MAPK (rabbit ...
• LFA-1 signals to promote actin polymerization and upstream migration in T cells

  Nathan H Roy, et al., bioRxiv - Cell Biology 2020

  Quote: ... Recombinant mouse ICAM-1-Fc and VCAM-1-Fc were purchased from R&D Systems. Antibodies for flow cytometery were from BioLegend ...
• Antisense modulation of IL7R splicing to control sIL7R expression in human CD4⁺ T cells

  Gaddiel Galarza-Muñoz, et al., bioRxiv - Immunology 2022

  Quote: ... The concentration of samples was extrapolated from standard curves of recombinant human IL7R-Fc chimera (R&D Systems, # 306-IR).
• Membrane phase separation drives organization at B cell receptor clusters

  Sarah A. Shelby, et al., bioRxiv - Biophysics 2022

  Quote: ... Recombinant VCAM-1/human Fc chimera protein was purchased from R&D Systems (R&D Systems Cat# 862-VC-100).
• N-cadherin dynamically regulates pediatric glioma cell migration in complex environments

  Dayoung Kim, James M Olson, Jonathan A Cooper, bioRxiv - Cancer Biology 2024

  Quote: ... was coated with 12.5 μg/ml recombinant human N-cad Fc chimera protein (Asp160-Ala724, R&D systems, 1388-NC) or 20 μg /ml poly-D-lysine in coating buffer (HBSS with 1 mM CaCl2 ...
• Trogocytic-molting of T-cell microvilli controls T-cell clonal expansion

  Jeong-Su Park, et al., bioRxiv - Immunology 2022

  Quote: ... Anti-human CD28 antibody and recombinant mouse ICAM-1/CD54 His-tag were purchased from R&D Systems (Minneapolis, MN, USA). Hybridoma cell lines for mouse anti-CD3 (145-2C11 ...
• Mfge8 attenuates human gastric antrum smooth muscle contractions

  Wen Li, et al., bioRxiv - Physiology 2020

  Quote: Recombinant human Mfge8 and recombinant human laminin subunit alpha-1 were purchased from R&D Systems, www.rndsystems.com ...
• The Role of Notch Signaling in Endometrial Mesenchymal Stromal/Stem-like Cells Maintenance

  Sisi Zhang, et al., bioRxiv - Cell Biology 2022

  Quote: Human recombinant JAG1 (R&D System) was fluorescently labeled with Alexa Fluor™ 488 Microscale Protein Labeling Kit (Thermo Scientific ...
• Superantigens promote Staphylococcus aureus bloodstream infection by eliciting pathogenic interferon-gamma (IFNγ) production that subverts macrophage function

  Stephen W. Tuffs, et al., bioRxiv - Microbiology 2021

  Quote: ... recombinant human IFNγ (R&D Systems) was also added at varying concentrations ...
• Non-canonical open reading frames encode functional proteins essential for cancer cell survival

  John R. Prensner, et al., bioRxiv - Cancer Biology 2020

  Quote: Recombinant human GDF15 (R&D Systems, Minneapolis ...
• The Polycomb group protein Ring1 regulates dorsoventral patterning of the mouse telencephalon

  Hikaru Eto, et al., bioRxiv - Developmental Biology 2019

  Quote: ... recombinant human BMP4 (R&D Systems) or CHIR-99201 (Wako) ...
• Wnt7a-releasing synthetic hydrogel enhances local skeletal muscle regeneration and muscle stem cell engraftment

  Woojin M. Han, et al., bioRxiv - Bioengineering 2019

  Quote: ... recombinant human Wnt7a (R&D systems) reconstituted in PBS was added to the PEG-4MAL precursor solution ...
• Investigating vaccine uptake dynamics of neutrophils using HIV-1 Envelope glycoprotein trimer

  Philip Y.X. Ngo, bioRxiv - Immunology 2022

  Quote: ... Recombinant human MBL (R&D systems) was reconstituted in PBS and serially diluted from 10μg/ml by two-fold dilution ...
• Therapeutic modulation of the blood-brain barrier and ischemic stroke by a bioengineered FZD₄-selective WNT surrogate

  Jie Ding, et al., bioRxiv - Physiology 2022

  Quote: ... Recombinant human WNT3A (R&D Systems) and recombinant human Norrin (R&D Systems ...
• Suspension Electrospinning of Decellularized Extracellular Matrix

  Sarah Jones, et al., bioRxiv - Bioengineering 2024

  Quote: ... Recombinant human VEGF (R&D Systems) was added to the basal media at 20 ng/mL immediately prior to cell treatment ...
• Self-organization, quality control, and preclinical studies of human iPSC-derived retinal sheets for tissue-transplantation therapy

  Kenji Watari, et al., bioRxiv - Neuroscience 2023

  Quote: ... recombinant human BMP4 (R&D Systems) was added at 1.5 nM (55 ng/ml)7 ...
• Inhibition of EGFR/ErbB does not protect against C. difficile toxin B

  Uswah Siddiqi, et al., bioRxiv - Microbiology 2024

  Quote: ... Recombinant human EGF (R&D Systems) was applied similarly as a positive control ...
• Semaphorin 4D induced inhibitory synaptogenesis restores benzodiazepine sensitivity in a mouse model of Status Epilepticus

  Susannah S. Adel, et al., bioRxiv - Neuroscience 2022

  Quote: ... A subset of slices were infected with only AAV9.hsyn.GFP and at DIV6 were treated with human Sema4D-Fc ectodomain/human Fc fusion protein (Sema4D-Fc; R&D Systems, #7470-S4) or Fc control protein (R&D Systems ...
• The connection between Rap1 and Talin1 in CD4⁺ T Lymphocytes

  Frederic Lagarrigue, et al., bioRxiv - Cell Biology 2021

  Quote: ... Recombinant mouse VCAM-1-Fc was from R&D Systems. Recombinant mouse ICAM-1 and VCAM-1-Fc were from BioLegend ...
• CrkL is required for donor T cell migration to GvHD Target organs

  Nathan H. Roy, et al., bioRxiv - Immunology 2019

  Quote: ... Recombinant mouse ICAM-1-Fc was from R&D Systems.
• More than a ligand: PD-L1 promotes oncolytic virus infection via a metabolic shift that inhibits the type I interferon pathway

  Jonathan J. Hodgins, et al., bioRxiv - Cancer Biology 2022

  Quote: ... recombinant mouse PD-1-Fc chimeric protein (R&D Systems) or human IgG1 control (R&D Systems) ...
• ALCAM-mediated synapses between DC1 and CD8 T cells are inhibited in advanced lung tumors

  Luciano G. Morosi, et al., bioRxiv - Immunology 2023

  Quote: ... recombinant mouse Fc-chimera proteins CD6 (rCD6, R&D Systems) and/or ICAM-1 (rICAM1 ...
• Decorin suppresses tumor lymphangiogenesis: A mechanism to curtail cancer progression

  Dipon K. Mondal, et al., bioRxiv - Cell Biology 2023

  Quote: ... Direct binding of decorin protein core to the soluble Fc tagged VEGFR3 (sVEGFR3-Fc, R&D systems, 349-F4), was performed using solid-phase binding assay ...
• Single-hit genome edition for expression of single-chain immunoglobulins by edited B cells

  Natsuko Ueda, et al., bioRxiv - Immunology 2022

  Quote: Cell surface staining for the anti-HER2 BCR was performed with Recombinant Human ErbB2/Her2 Fc Chimera Protein (R&D Systems) and APC anti-His Tag antibody (Biolegend ...
• Essential roles of plexin-B3⁺ oligodendrocyte precursor cells in the pathogenesis of Alzheimer’s disease

  Naomi Nihonmatsu-Kikuchi, et al., bioRxiv - Neuroscience 2020

  Quote: ... the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp) with a C-terminal 6-His tag) (R&D systems) overnight.
• Diverging maternal and infant cord antibody functions from SARS-CoV-2 infection and vaccination in pregnancy

  Emily H. Adhikari, et al., bioRxiv - Immunology 2023

  Quote: ... Recombinant Fc receptors (FcγRIIIa/CD16a, FcγRIIa/CD32a H167, FcγRIIb/CD32b, Neonatal Fc receptor/FcRN) (R&D Systems) were labeled with PE (Abcam ...
• Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling

  Beatriz Garcia-Diaz, et al., bioRxiv - Neuroscience 2019

  Quote: Mouse EphrinB3-Fc fragments and human Fc were purchased by R&D Systems. The soluble forms of EphrinB3-Fc ...
• Identification of a new regulation pathway of EGFR and E-cadherin dynamics

  Veronique Proux-Gillardeaux, et al., bioRxiv - Cell Biology 2020

  Quote: 0.3M of purified proteins (recombinant human E-cadherin 8505 and recombinant human EGFR 344-ER R&D system) were mixed and incubated in 100μl of lysis buffer (25 mM TrisHCl pH 7.5 ...
• PD-L1 glycosylation and its impact on binding to clinical antibodies

  Julius Benicky, et al., bioRxiv - Cancer Biology 2020

  Quote: ... with C-terminal 6xHis tag produced in HEK293 cells and human recombinant PD-L1-Fc chimera protein produced in mouse NS0 cells were obtained from R&D Systems, Minneapolis ...
• Identification of a lymphocyte minor histocompatibility antigen in Mauritian cynomolgus macaques

  Jason T. Weinfurter, et al., bioRxiv - Immunology 2020

  Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
• Systematic perturbation screens decode regulators of inflammatory macrophage states and identify a role for TNF mRNA m6A modification

  Simone M Haag, et al., bioRxiv - Immunology 2024

  Quote: ... In vitro TNFα-blocking studies were performed using recombinant human TNFRII/TNFRSF1B-Fc chimera protein (1 µg / ml) (726-R2-050, R&D Systems) for 18 h.
• Endothelial cell signature in muscle stem cells validated by VEGFA-FLT1-AKT1 axis promoting survival of muscle stem cell

  Mayank Verma, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 100 ng/ml recombinant FLT1-FC (R&D Systems, 7756-FL), 1 µg/ml anti-FLT1 monoclonal antibody (Angio-Proteomie ...
• Delivery of CAR-T Cells in a Transient Injectable Stimulatory Hydrogel Niche Improves Treatment of Solid Tumors

  Abigail K. Grosskopf, et al., bioRxiv - Bioengineering 2021

  Quote: B7H3 CAR was detected using recombinant B7H3-Fc (R&D Systems) fluorescently labeled with the DyLight 650 Microscale Antibody Labeling Kit (Thermo Fisher) ...
• Cytotoxic CD4 Development Requires CD4 Effectors to Concurrently Recognize Local Antigen and Encounter Infection-Induced IL-15

  Priyadharshini Devarajan, et al., bioRxiv - Immunology 2022

  Quote: Recombinant Mouse IL-15R alpha Fc Chimera Protein (R&D Systems) and recombinant Mouse IL-15 (Tonbo Biosciences ...
• CD155 blockade enhances allogeneic natural killer cell-mediated antitumor response against osteosarcoma

  Monica M Cho, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and recombinant mouse IL-15Ralpha Fc chimera protein (R&D Systems), in RPMI medium (Corning Life Sciences ...
• WFIKKN2 is a bifunctional axon guidance cue that signals through divergent DCC family receptors

  Kelsey R. Nickerson, et al., bioRxiv - Developmental Biology 2023

  Quote: ... recombinant mNope-ECD-Fc protein (Biotechne/R&D Systems, 1294-NP) was added at 25 μg/ml (≍ 0.2 μM ...
• Microglia-specific promoter activities of human HexB gene

  Sahil Shah, et al., bioRxiv - Neuroscience 2021

  Quote: ... recombinant human M-CSF (R&D Systems), 100 μg/mL streptomycin ...
• A secreted Echinococcus multilocularis activin A homologue promotes regulatory T cell expansion

  Justin Komguep Nono, Manfred B. Lutz, Klaus Brehm, bioRxiv - Immunology 2019

  Quote: ... Recombinant human TGF-β1 (R&D Systems) was used as positive control ...
• Cooperative regulation of adherens junction elongation through Epidermal Growth Factor Receptor (EGFR) activation

  Chaoyu Fu, et al., bioRxiv - Biophysics 2021

  Quote: ... Recombinant Human EGF Protein (R&D Systems). Erlotinib hydrochloride (500nM ...
• Therapeutic modulation of the blood-brain barrier and ischemic stroke by a bioengineered FZD₄-selective WNT surrogate

  Jie Ding, et al., bioRxiv - Physiology 2022

  Quote: ... and recombinant human Norrin (R&D Systems) were used as response comparators ...