Propane 1 2 Diyl Diacetate citations for R&D Systems

Quote: ... Human T cells were also cultured under conditions where 5 µg/ml neutralizing anti-IL-2 antibody (R&D Systems, Minneapolis, MN, USA) or 10 ng/ml IL-12 (PeproTech ...

SARS-CoV-2 transmission via apical syncytia release from primary bronchial epithelia and infectivity restriction in children epithelia

Guillaume Beucher, et al., bioRxiv - Microbiology 2021

Quote: Human IL-29/IL-28B (IFN-lambda 1/3) concentration in SARS-CoV-2 infected epithelium basal media was quantified using ELISA technics following manufacturer’s recommendations (R&D systems, Minneapolis, USA). 100 μl of media was used for each point.

Inhibition of SARS-CoV-2 infection in human cardiomyocytes by targeting the Sigma-1 receptor disrupts cytoskeleton architecture and contractility

José Alexandre Salerno, et al., bioRxiv - Cell Biology 2021

Quote: ... IL-8 and TNF-α were quantified in the supernatants from uninfected and SARS-CoV-2-infected hiPSC-CMs by ELISA (R&D Systems), following manufacturer’s instructions ...

Mesenchymal tumor organoid models recapitulate rhabdomyosarcoma subtypes

Michael T. Meister, et al., bioRxiv - Cancer Biology 2022

Quote: ... culture medium was supplemented with 0.1 to 0.5 % BME (Cultrex Reduced Growth Factor Basement Membrane Extract, type 2, Pathclear, R&D Systems, 3533-005-02). We observed batch-to-batch variations of this product ...

SARS-CoV-2 Spike protein suppresses CTL-mediated killing by inhibiting immune synapse assembly

Anna Onnis, et al., bioRxiv - Immunology 2022

Quote: ... were incubated for 30 min at 20°C in 50 μl RPMI-HEPES in the absence of BCS with 2 μg/ml anti-ACE2 antibody (R&D Systems, #AF933) (Hoffmann et al. ...

The Role of EBP50 in Regulating Endothelial-To-Mesenchymal Transition in Pulmonary Hypertension

Anastasia Gorelova, et al., bioRxiv - Cell Biology 2022

Quote: ... Samples were blocked in 2% goat serum for 45 mins at room temperature and incubated with anti-Snail (R&D Systems, Minneapolis, MN), anti-Zeb1 (Invitrogen ...

A novel anti-inflammatory treatment for bradykinin-induced sore throat or pharyngitis

Victor Leyva-Grado, Pavel Pugach, Nazlie Latefi, bioRxiv - Immunology 2021

Quote: ... Cyclooxygenase-2-derived prostaglandin E2 (PGE2) and LTB4 release were measured with enzyme-linked immunosorbent assay kits (ELISA; R&D systems, Minneapolis, MN) according to the manufacturer’s instructions ...

Competition between distinct ApoE alleles and mCRP for the endothelial receptor CD31 differentially regulates neurovascular inflammation and Alzheimer’s disease pathology

Zhengrong Zhang, et al., bioRxiv - Neuroscience 2021

Quote: ... 2) anti-CD31 antibodies (#550274, BD Biosciences, San Jose, CA, USA; #ab28364, Abcam, Cambridge, MA, USA; #BBA7, R&D systems, Minneapolis, MN, USA); 3 ...

A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes

Tanya Grancharova, et al., bioRxiv - Cell Biology 2021

Quote: ... Media was replaced after 48 hours (Day 2) with RPMI-1640 supplemented with B27 without insulin and 5 µM IWP2 (R&D Systems #3533). Media was again replaced after an additional 48 hours (Day 4 ...

Inhibitory input directs astrocyte morphogenesis through glial GABA_BR

Yi-Ting Cheng, et al., bioRxiv - Neuroscience 2023

Quote: ... 3mg of nuclear lysates were used for IP with 2 μg IgG or 5 μg primary antibodies (normal mouse IgG, Santa Cruz Biotechnology, sc-2025; normal rabbit IgG, R&D Systems, AB-105-C ...

Mammalian cells internalize bacteriophages and utilize them as a food source to enhance cellular growth and survival

Marion C. Bichet, et al., bioRxiv - Microbiology 2023

Quote: ... Murine IP-10 production was measured from 100 μL of the supernatant from the BMDMs using Mouse CXCL10/IP-10/CRG-2 Duo Set ELISA (R&D systems, #Dy466) according to the manufacturer’s protocol.

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