Labshake search
Citations for R&D Systems :
901 - 950 of 3120 citations for Anti Syndecan 4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2020Quote: ... on ice for 30 minutes and then stained on ice for 1 hour with a 1:100 dilution of goat anti-Epha1 antibody (R&D systems #AF3034). After two washes with PBS/10% DS ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were then blocked with odyssey PBS for 1 hour at room temperature and incubated with the anti-hERBB2/Her2 AF488 antibody (1:200, R&D systems biotechne) overnight at 4 °C ...
-
bioRxiv - Neuroscience 2021Quote: ... 2) anti-CD31 antibodies (#550274, BD Biosciences, San Jose, CA, USA; #ab28364, Abcam, Cambridge, MA, USA; #BBA7, R&D systems, Minneapolis, MN, USA); 3 ...
-
bioRxiv - Neuroscience 2021Quote: ... the astrocyte-enriched cell suspension was incubated for 20 minutes at room temperature on a dish coated with anti-HepaCAM antibody (R&D Systems MAB4108) to bind astrocytes ...
-
bioRxiv - Microbiology 2021Quote: ... One million cells were re-suspend in 100 μl PBS and either immediately incubated with 0.25 μg anti-ACE2 antibody (R&D systems, Cat.# AF933) or first incubated at 37 °C for 6 hours with shaking to allow recovery of cell surface proteins after trypsinization ...
-
bioRxiv - Microbiology 2021Quote: ... LAD2 cells were prior-treated with 0.25% trypsin (without EDTA) for 10 min at 37 °C or prior-blocked with anti-ACE2 antibody (5 μg/mL, R&D Systems, AF933) for 2 h at 37 °C before the incubation with Spike-RBD protein.
-
bioRxiv - Developmental Biology 2021Quote: ... Cells were removed from adherent cultures using AssayComplete cell dissociation reagent (Eurofins #92-0009) and incubated with a polyclonal anti-ALK2 antibody (R&D Systems #AF637) or a polyclonal anti-ALK3 antibody (Sino Biological #50078-RP02) ...
-
bioRxiv - Neuroscience 2023Quote: ... PGRN measurements were made using the Mesoscale Discovery (MSD) platform as previously described (106) using either a biotinylated anti-hPGRN antibody (R&D Systems, BAF2420) or anti-mPGRN antibody (R&D Systems ...
-
bioRxiv - Neuroscience 2023Quote: ... Trem2 measurements were made using the Mesoscale Discovery (MSD) platform as previously described (29, 105) using a biotinylated anti-mTrem2 polyclonal antibody (R&D Systems BAF1729) as capture antibody and a sulfo-tagged detection antibody (sheep anti-mouse Trem2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... POSTN protein expression in the medium was confirmed using an ELISA assay with an anti-POSTN polyclonal antibody (R&D Systems, Cat # AF3548). 3T3-CTL cells were used to generate “control” conditioned medium (CMCTL) ...
-
bioRxiv - Cell Biology 2023Quote: ... and then incubated with anti-8-oxo-dG antibody (1:400 in 1% goat serum; R&D Systems, 4354-MC-050, Lot# P275856) for 1 hour ...
-
bioRxiv - Neuroscience 2024Quote: ... MaxiSORP 96-well plates were coated with 1μg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (R&D Systems; clone 237920) at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... IL-4 (R&D Systems Cat#204-IL-050, 20ng/mL), αIgG (Agilent Cat#A042402-2) ...
-
bioRxiv - Immunology 2021Quote: ... 10 ng/mL IL-4 from R&D Systems (404-ML), and 3 μg/mL anti-IFN-γ was added ...
-
bioRxiv - Microbiology 2021Quote: ... 5 ng/mL BMP-4 (314-BP-050, R&D Systems), 50 ng/mL VEGF (293-VE-500 ...
-
bioRxiv - Immunology 2020Quote: ... IL-4 and IL-13 were purchased from R&D Systems. Acid Phosphatase ...
-
bioRxiv - Cancer Biology 2021Quote: Recombinant 52-kDa pro-cath-D (4 µM; R&D Systems) was auto-activated to 51-kDa pseudo-cath-D in 0.1 M Na-acetate buffer (pH 3.5) ...
-
bioRxiv - Biochemistry 2022Quote: ... 20 ng/mL bone morphogenetic protein 4 (BMP4, R&D Systems), and 10 ng/mL fibroblast growth factor-basic (bFGF ...
-
bioRxiv - Immunology 2020Quote: ... 3 and 4 were measured using Luminex technology (R&D Systems), according to the manufacturer’s protocols ...
-
bioRxiv - Developmental Biology 2019Quote: ... 4 ng human recombinant IGF2 (292-G2-050, R&D Systems) was used as positive control ...
-
bioRxiv - Cell Biology 2021Quote: ... 30 ng/ml Bone morphogenic protein-4 (BMP4) (R&D systems), 1.5 μM CHIR99021 (Stemgent) ...
-
bioRxiv - Microbiology 2020Quote: ... and 4,000 U/ml of rh IL-4 (R&D Systems) and cultured for 5 days ...
-
bioRxiv - Neuroscience 2022Quote: ... or IL-4 (20 ng/mL, 24 h, R&D Systems). Following stimulation ...
-
bioRxiv - Developmental Biology 2022Quote: ... BMP-4 (40 ng/ml, R&D Systems #5020-BP-010) or CHIR99021 (3 μM ...
-
bioRxiv - Cancer Biology 2022Quote: ... The caspase 4 inhibitor (Z-YVAD-FMK, FMK005, R&D systems) was used in further experiments at the dose of 50 µM ...
-
bioRxiv - Molecular Biology 2023Quote: ... and growth factors: 5 ng/ml BMP-4 (R&D Systems), 9 ng/ml Activin A (R&D Systems) ...
-
bioRxiv - Bioengineering 2023Quote: ... and 20 ng/mL human recombinant FGF-4 (R&D Systems). After 9 to 13 days ...
-
bioRxiv - Microbiology 2024Quote: ... and IL-4 (R&D Systems, Cat# 6507-IL-025/CF) in complete RPMI ...
-
bioRxiv - Immunology 2024Quote: ... TNFα and IL-4 using ELISA kits from R&D Systems in accordance with the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Blots were incubated for 1 hour with HRP-conjugated secondary antibodies diluted in 5% skim milk: anti-mouse (HAF007, R&D Systems, 1:25,000); anti-rabbit (111-035-144 ...
-
bioRxiv - Neuroscience 2022Quote: ... Non-specific blocking for 30 minutes in 8% horse serum in KPBS was completed before incubation in primary antibody (anti-HPLN1 1:150, R&D Systems 2608-HP) for 48 hours at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) with 0.1% Tween 20) for 30 minutes and probed with primary antibodies: polyclonal goat anti-C3d (R&D systems, AF2655, 1:1000), polyclonal sheep anti-Sez6L2 (R&D Systems ...
-
bioRxiv - Pathology 2022Quote: ... blocking-and antibody-incubations were performed in TBS + 0,1% Tween20 + 5% milk. Following primary antibodies (Suppl. Table 1) were used: anti-NRP1 (1:1000, R&D Systems, Cat. No. AF566), anti-NRP2 (1:1000 ...
-
bioRxiv - Cell Biology 2022Quote: ... and stained for 30 minutes on ice with a 1:50 dilution of AlexaFluor-647 conjugated anti-LTB4R antibody (R&D Systems; catalog #: FAB099R) in FACS buffer (0.5% bovine serum albumin and 0.05% sodium azide in PBS) ...
-
bioRxiv - Cancer Biology 2022Quote: ... +/-1 µg/mL anti-CD28 (CD28.2) antibody (eBioscience/Thermo 16-0289-81) or recombinant human CD58-Fc chimera protein (R&D Systems 10068-CD-050) at 37 ºC for 2 hours ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by incubation with anti-CD31 primary antibody (1:200, #550274, BD Biosciences, San Jose, CA, USA; #BBA7, R&D systems, Minneapolis, MN, USA) and Alexa Fluor 594-conjugated secondary antibody.
-
bioRxiv - Biochemistry 2023Quote: ... Proteins tagged with the TAP (tandem affinity purification) or HA (hemagglutinin) tag were detected with PAP or an anti-HA antibody (Sigma, Taufkirchen, Germany; R&D Systems, Minneapolis, USA) and a horseradish peroxidase (HRP)-coupled secondary antibody and CheLuminate-HRP ECL solution (Applichem ...
-
bioRxiv - Immunology 2023Quote: ... Protein concentration was measured with BCA assay and 350 µg of lysates were incubated with 5 μg of anti-Siglec-E antibody (polyclonal goat IgG - R&D Systems – cat. AF5806) or goat IgG for 2h at 4°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... P0 were fixed and histologically sectioned and were stained with antibodies to detect Wnt4 (anti-Wnt4, R&D systems, cat# AF475, dilution 1:100), Wnt9b (anti-Wnt9b ...
-
bioRxiv - Cell Biology 2023Quote: The following antibodies were used: monoclonal mouse anti-human CXCR4 (clone 44717) and phycoerythrin-conjugated human CXCR4 (clone 12G5; both from R&D Systems, Minneapolis, MN); purified mouse anti-human CD3 (clone HIT3a ...
-
bioRxiv - Biochemistry 2024Quote: ... Confirmation of ETB gene expression was confirmed in cell populations by surface staining with antibodies (anti-HA tag AlexaFluor 488 conjugated mouse IgG, R&D Systems, cat # IC6875G) in combination with flow cytometry ...
-
bioRxiv - Immunology 2024Quote: ... anti-AMPKα2 (#AF2850, diluted at 1:1000) and anti-IL6 (#AB-206-NA, diluted at 1:500) antibodies from R&D Systems (Minneapolis, MN, USA). Secondary antibodies used for immunofluorescence labelling of fixed cells were goat anti-rabbit antibodies conjugated to Alexa Fluor 488 (#11034 ...
-
bioRxiv - Biochemistry 2021Quote: ... The following antibodies were used as detector antibodies for tetraspanins: ab58989 (R&D Systems), 556019 (BD ...
-
bioRxiv - Cancer Biology 2020Quote: ... and experimental samples were stained with a 30 nM concentration of either commercially available anti-human GPC3 Phycoerythrin (PE)-conjugated mouse antibody (R&D Systems, Inc. Minneapolis, MN) or TAB-H14 antibody conjugated to Alexa Fluor 488 (AF-488 ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were detected on a Li-Cor Odyssey Blot Imager using the following primary and secondary antibodies: anti-IL-1β (R&D systems, AF-201-NA), anti-GFP (Clontech ...
-
bioRxiv - Developmental Biology 2022Quote: ... In-house purified rhFGF10 was confirmed by western blot analysis using anti-FGF10 antibody and compared to commercially purchased rhFGF10 (R&D Systems, Cat#345-FG) to test/validate activity based on the efficiency to phosphorylate ERK1/2 in an A549 alveolar epithelial cell line (ATCC ...
-
bioRxiv - Cell Biology 2019Quote: ... Antibodies used for ChIP (3 μg per reaction) were mouse monoclonal IgG2a including: 1) anti-NR1H3 (clone PPZ0412, R&D Systems catalog PP-PPZ0412-00), 2 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-1β in conditioned medium was analysed by western blotting on nitrocellulose membrane with a goat anti-mouse IL-1β antibody (AF-401-NA, R&D systems, 1:1000 dilution), donkey anti-goat horseradish peroxidase-conjugated secondary antibody (A15999 ...
-
bioRxiv - Neuroscience 2023Quote: The following antibodies were used for immunofluorescence studies (dilutions for each antibody are indicated in parentheses): chicken polyclonal anti-neurofascin (1:500; R&D Systems Cat# AF3235, RRID:AB_10890736), chicken polyclonal anti-MAP2 (1:1000 ...
-
bioRxiv - Developmental Biology 2023Quote: Flow cytometry performed within 1-6 days investigated NOTCH1 expression using anti-NECD1 antibody AF5267 and Notch ligand binding for DLL1-Fc (R&D Systems, Inc., Minneapolis, MN) and DLL4-Fc (Aro Biosystems ...