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Citations for R&D Systems :
851 - 900 of 1044 citations for 4 Tert Butyldimethylsilyl Oxy Methyl 2 Iodothiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
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bioRxiv - Neuroscience 2021Quote: ... This was then followed by an overnight incubation at 4°C with appropriately diluted primary antibodies: anti-CD31 (R&D Systems, clone AF3628, 1:250); anti-Lyve-1(ReliaTech GmbH ...
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bioRxiv - Physiology 2021Quote: ... Cells were then incubated overnight at 4°C with goat anti-mouse platelet endothelial cell adhesion molecule (CD31/PECAM-1) primary antibody (R&D Systems cat. No. AF3628) diluted in antibody buffer (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... samples were incubated overnight at 4°C with mouse anti-mCRP antibody (1:50, 3H12) and either goat anti-CD31 antibody (1:200, #AF3628, R&D systems, Minneapolis, MN, USA) for mouse samples or rabbit anti-CD31 antibody (1:200 ...
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bioRxiv - Immunology 2021Quote: ... 109-006-127), 250ng/ml human recombinant MEGACD40L (Enzo, ALX-522-110) and 20ng/ml Recombinant human IL-4 (R&D system, 204-IL-010). Cells were collected at specific time points for analysis.
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bioRxiv - Immunology 2021Quote: ... The membranes were then washed with PBST and incubated at 4°C overnight with goat anti-mouse IL-1β (250 ng ml−1; R&D Systems, AF-401-NA), mouse anti-mouse NLRP3 (1 μg ml−1 ...
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bioRxiv - Bioengineering 2022Quote: Transduced/Transfected and sorted hGICs harbouring either Lentivirus-MGT#1 or PiggyBac-MGT#1/4 were seeded as single cells into 6-well plates in RHB-A medium supplemented with 10ng/ml TNFa (R&D Systems, 210-TA-020) or without TNFa as control ...
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bioRxiv - Cancer Biology 2023Quote: Ascites collected from RD and IF mice (n=4/ group) were pooled and diluted 1:3 and applied in the adipokine array (ARY-013, R&D systems, Minneapolis, MN, USA). The proteome profiler adipokine array detects 38 adipokines (Table S4 ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked using 5% donkey serum or 5% BSA in PBST for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in the blocking solution: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasma concentrations of fatty acid binding protein 4 (FABP4) and leptin were determined using the xMAP technology Luminex assays (R&D Systems, Minneapolis, MN, USA) and the Luminex MAGPIX System (Luminex Corp. ...
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bioRxiv - Molecular Biology 2024Quote: ... non-fat dried milk in TTBS at room temperature for 1 h and incubated overnight at 4 °C with specific anti-IL-1β (R&D Systems, Minneapolis, MN, USA), anti-phospho-NF-κB p65 (Ser536 ...
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bioRxiv - Immunology 2024Quote: ... 1 × 105 B cells were added to each well in 6 mL of B cell media containing either IL-4 (R&D Systems, 1 ng/mL) or IL-21 (R&D Systems ...
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bioRxiv - Developmental Biology 2020Quote: ... and the combination treatment of Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3). At Day2 ...
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bioRxiv - Microbiology 2021Quote: ... DY210) were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... serum was incubated for 2 h with polyclonal sheep IgG anti-LL-37 (2.5 – 20 μg ml−1; R&D Systems) or anti-hNP-1 (2.5 – 20 μg ml−1 ...
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bioRxiv - Immunology 2020Quote: ... 2 × 105 cells/ well in a 24 well plate were seeded on 10ug/ml fibronectin-coated cover slips (R&D systems) in 500ul 20ng/ml macrophage colony stimulating factor (MCSF ...
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bioRxiv - Immunology 2021Quote: ... In another experiment mice were treated intravenously 2 days post infection with 12.5 μg/kg recombinant murine IL-23 (1887-ML-010/CF, R&D Systems) for 3 consecutive days.
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bioRxiv - Cell Biology 2021Quote: Isolated cardiac fibroblasts at Passage (P)2 were treated with 10 ng/mL TGF-β1 (R&D Systems Cat #240-B) in Fibroblast Growth Medium 3 for four days to assess myofibroblast differentiation ...
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bioRxiv - Bioengineering 2022Quote: ... Flow cells were washed and incubated with mouse anti-human VE-cadherin (2 μg/ml clone 123413, R&D Systems, US) followed by goat anti-mouse lgG AF® 594 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Fibers were incubated in plating medium consisting of 15% Fetal Bovine Serum (Hyclone) and 2 ng/ml bFGF (R&D Systems) in DMEM.
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bioRxiv - Immunology 2020Quote: ... Levels of cytokines in culture supernatants were assessed using mouse IL-2 and IFN-gamma DuoSet ELISA kits (R&D Systems), according to manufacturers’ instructions.
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bioRxiv - Neuroscience 2022Quote: ... Group 2 ligands were derived from human transcriptomic data and included: TGFβ2 (R&D Systems, Cat. 302-B2-002, 5ng/mL), NLGN1 (Sino Biological ...
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bioRxiv - Pathology 2022Quote: Antibody responses were measured against recombinant beta-SARS-CoV-2 B.1.351 spike protein (S) and its receptor binding domain (RBD) (10777-CV and 10735-CV, R&D Systems) by ELISA as previously described [23] ...
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bioRxiv - Cancer Biology 2020Quote: ... Dot blots were washed three times with PBST and incubated with 15 μl of DAB (3,3’-Diaminobenzidine) chromogen (2 ml of DAB chromogen (860001, R&D Systems) diluted in DAB chromogen buffer (860005 ...
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bioRxiv - Microbiology 2020Quote: ... Fecal lysates were analyzed using a mouse lipocalin-2/NGAL ELISA kit following the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
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bioRxiv - Cell Biology 2022Quote: ... 50 µl 1.2% barium chloride (w/v in H2O) with or without 2 µg of Osteopontin neutralizing antibody (AF808, R&D Systems) was injected into a TA muscle ...
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bioRxiv - Biochemistry 2022Quote: ... cells were treated for 2 hours prior to infection with the IC50 concentration of anti-ACE2 antibody (R&D Systems: AF933) determined for the cell lines used ...
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bioRxiv - Molecular Biology 2023Quote: ... according to manufacturer’s instructions (Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA # DY466 and Mouse TNF-alpha DuoSet ELISA # DY410, R&D systems). Signals were detected by using TMB solution (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Relative expression was calculated against a 2% input control.Samples were then normalized to uninfected cells stimulated with universal type 1 interferon (R&D Systems).
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bioRxiv - Developmental Biology 2023Quote: ... media was changed daily with hindgut medium (RPMI 1640, 1X GlutaMAX, 2% defined FBS, 2µM CHIR99021 and 500ng/ml FGF4 (R&D Systems) until day 8 ...
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bioRxiv - Cell Biology 2023Quote: ... SH-SY5Y cells were pre-treated for 16 h with Type I Interferon (1000 Units/mL; R&D Systems, 11200-2) and treated with HMW Poly(I:C ...
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bioRxiv - Cancer Biology 2023Quote: ... into 40 ml of R10 media supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050). Cells were passaged twice per week and feeder cells were added in a 1:1 ratio after 7 days to maintain stimulation ...
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bioRxiv - Immunology 2022Quote: ... were determined by the CXCL2/MIP-2 DuoSet enzyme linked immunosorbent assay (ELISA) kit to the manufacturer’s instructions (R&D Systems). Horseradish peroxidase-catalyzed color reaction were initiated by addition of the TMB Substrate Kit (Biolegend ...
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bioRxiv - Developmental Biology 2024Quote: Human or rhesus iPSCs were dissociated using Accutase and seeded onto Matrigel coated plates at a density of 50,000/cm2 in cold mTESR1 supplemented with 2% Cultrex (R&D Systems), 10 µM Y-27632 ...
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bioRxiv - Molecular Biology 2024Quote: ... accurate ACE2 expression was controlled on western blot probed with anti-ACE2 antibody (Human ACE-2 Antibody, AF933, R&D systems). ACE2-positive cells (70-80% confluence ...
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bioRxiv - Cell Biology 2024Quote: ... The organoids were encapsulated in 50% Cultrex basement membrane extract type 2 (BME-II) (R&D Systems; catalog #3533-010-02) and maintained in advanced DMEM/F12 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... cells were pelleted by centrifugation and media was replaced with plasmablast generation media: 10 ng/mL IL-2 (R&D Systems), 50 ng/mL IL-6 (Peprotech) ...
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bioRxiv - Microbiology 2024Quote: ... Isolated naive CD4+ T cells were resuspended in isolation media supplemented with recombinant IL-2 (R&D Systems, #202-IL-050) at 10 ng/mL (growth media ...
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bioRxiv - Bioengineering 2024Quote: ... 5% CO2 in R10 containing 60 IU mL-1 recombinant human IL-2 (R&D Systems, CAT#202-IL-059/CF). The following day ...
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bioRxiv - Immunology 2024Quote: ... Abundance of LCN-2 and IL-17A/F were measured by Quantikine ELISA assays from R&D Systems (Minneapolis, MN, USA). BALF (50 μL ...
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bioRxiv - Immunology 2024Quote: ... 5×104 labeled cells were resuspended in B cell media (RPMI, 5%FCS, 50μM 2-mercaptoethanol and 2mM glutamine) and stimulated with 50ng/ml CD40L (R&D Systems), 50ng/ml IL-4 (STEMCELL Technologies) ...
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bioRxiv - Immunology 2024Quote: ELISA kits were used to quantified serum ferritin (Christal Chem) and soluble mouse CD25/IL-2 alpha receptor (R&D Systems) following manufacturers instruction.
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bioRxiv - Neuroscience 2024Quote: ... and reseeded on laminin-521 coated plates at a split ratio of 1:2.5 in CPN medium supplemented with 20 ng/mL fibroblast growth factor 2 (FGF2; R&D Systems) and 10 µM Y-27632 ...
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bioRxiv - Neuroscience 2024Quote: ... small molecules (CHIR99021[2 mM, Axon, Groningen, Netherlands]; SB-431542[10 mM, Axon, Groningen, Netherlands]; noggin[0.5 mg/ml, R&D Systems R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... the buffer was switched to imaging media with either 1mM or 11mM glucose with treatment (CB-100: 5μg/mL, nidogen-2 (Biotechne R&D Systems)) and incubated for 30 minutes prior to acquiring a treatment image ...
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bioRxiv - Cell Biology 2024Quote: ... the buffer was switched to imaging media with either 1mM or 11mM glucose with treatment (CB-100: 5μg/mL, nidogen-2 (40ng/mL, Biotechne R&D Systems)) and incubated for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatants were used with the Human Phospho-EphB4 DuoSet IC ELISA kit (#DYC4057-2, R&D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... 2 µM parkin (either full-length or truncated parkin) were incubated in the presence of 10 µM UbVS (R&D Systems) in 30 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated with horse serum (7% in PBS) for 20 minutes prior to overnight incubation at 4°C with goat anti- intracellular adhesion molecule-1 (ICAM1) (R&D Systems, Inc, Minneapolis, MN, USA), rabbit anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Physiology 2024Quote: ... 50μL serum was 1:4 diluted with 150 μL dilution buffer to measure glucagon concentration using commercial ELISA kits (Mouse, DGCG0, R&D Systems; human, D711361, Sangon Biotech). All procedures were performed by following the manufacturer’s protocol.