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8601 - 8650 of 10000+ citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020

• Comprehensive multi-platform tyrosine kinase profiling reveals novel actionable FGFR aberrations across pediatric and AYA sarcomas

  Ashleigh M Fordham, et al., bioRxiv - Cancer Biology 2023

  Quote: Human Phospho-Receptor Tyrosine Kinase Array Kit (R&D Systems, Cat#ARY001B) and Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems ...
• IL-33 controls IL-22-dependent antibacterial defense by modulating the microbiota

  Ivo Röwekamp, et al., bioRxiv - Immunology 2023

  Quote: ... ATP and IL-33 were measured via ELISA (eBioscience, R&D Systems) according to the manufacturer’s instructions.
• Active synthesis of type I collagen homotrimer in Dupuytren’s fibrosis is unaffected by anti-TNF-α treatment

  Kate Williamson, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse anti-human TNF-α antibody (MAB610, R&D Systems, Minneapolis, USA) or mouse IgG1 Isotype Control (MAB002) ...
• Structure-activity studies of Streptococcus pyogenes enzyme SpyCEP reveal high affinity for CXCL8 in the SpyCEP C-terminal

  Max Pearson, et al., bioRxiv - Microbiology 2023

  Quote: ... 37 °C incubation of 111.1 pmol human LL-37 (R&D Systems) with SpyCEP constructs at a 1:10 molar ratio in favour of LL-37 in a final volume of 20 μl ...
• Long-term breast cancer response to CDK4/6 inhibition defined by TP53-mediated geroconversion

  Rei Kudo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... cell viability was measured by Resazurin (R&D Systems, Minneapolis, MN, USA) as described previously (20) ...
• Cancer-associated fibroblasts promote drug resistance in ALK-driven lung adenocarcinoma cells by upregulating lipid biosynthesis

  Ann-Kathrin Daum, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The following compounds were purchased from R&D Systems (Minneapolis, MN, USA): HGF ...
• Propagation of neuronal micronuclei regulates microglial states

  Sarasa Yano, et al., bioRxiv - Neuroscience 2023

  Quote: ... The recombinant Reelin (3820-MR/CF) was purchased from R&D Systems. The full-length mouse Reelin cDNA and pCAGGS1 plasmid containing a CAG promoter were kindly provided by Dr ...
• Cellular Allostatic Load is linked to Increased Energy Expenditure and Accelerated Biological Aging

  Natalia Bobba-Alves, et al., bioRxiv - Cell Biology 2023

  Quote: Mycoplasma testing was performed according to the manufacturer’s instructions (R&D Systems) on media samples at the end of lifespan for each treatment and cell line used ...
• Non-glycosylated IGF2 prohormones are more mitogenic than native IGF2

  Pavlo Potalitsyn, et al., bioRxiv - Biochemistry 2023

  Quote: ... His-tagged ALS protein (R&D Systems, 10 ng/well, 1.5 µM) was immobilized in wells via neutravidin and the nickel-charged-NTA-decorated iBodies® (IOCB Tech ...
• Secreted soluble factors from tumor-activated mesenchymal stromal cells confer platinum chemoresistance to ovarian cancer cells

  Yifat Koren Carmi, et al., bioRxiv - Cancer Biology 2023

  Quote: The human XL cytokine array kit (R&D Systems, Minneapolis, MN, USA) was utilized to detect relative levels of various cytokines ...
• In Breast Cancer ZMIZ1 Co-Regulates E2F2 as Part of the Estrogen Receptor-Mediated Cell-Cycle Response

  Weiye Zhao, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The specific antibody targeting ZMIZ1 was purchased from R&D Systems (AF8107) and used at a concentration of 2 μg/ml (1:250 dilution) ...
• Extracellular vesicle cargo metabolome changes in response to the mesenchymal stromal cell microenvironment and influences cell quiescence and activation in a human breast cancer cell model

  Sara Bartlome, et al., bioRxiv - Cell Biology 2022

  Quote: The Proteome ProfilerTM Human XL Cytokine Array Kit (R&D Systems Inc) was employed to determine the relative levels of cytokines in the supernatants of pQ-MSCs and pA-MSC cultures and their respective EV populations.
• Neutrophil extracellular trap stabilization by platelet factor 4 reduces thrombogenicity and endothelial cell injury

  Anh T. P. Ngo, et al., bioRxiv - Cell Biology 2023

  Quote: ... or tumor necrosis factor alpha (TNFα, 20 ng/mL, R&D systems) were used as positive controls.
• SCCA1/SERPINB3 promotes suppressive immune environment via STAT-dependent chemokine production, blunting the therapy-induced T cell responses

  Liyun Chen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and mouse S100A8/S100A9 Heterodimer DuoSet ELISA kit from R&D Systems. Mouse GRO gamma ELISA Kit was obtained from Abcam ...
• Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis

  Biren M. Dave, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 10 ng/ml NT3 (R&D Systems, cat# 267-N3-025/CF) and 100 ng/ml BDNF (R&D Systems ...
• Human Retinal Ganglion Cells Respond to Evolutionarily Conserved Chemotropic Cues for Intra Retinal Guidance and Regeneration

  Murali Subramani, et al., bioRxiv - Developmental Biology 2023

  Quote: ... All growth factors and small molecules were purchased from R&D Systems, Sigma and Milteny Biotech.
• Tozorakimab (MEDI3506): a dual-pharmacology anti-IL-33 antibody that inhibits IL-33 signalling via ST2 and RAGE/EGFR to reduce inflammation and epithelial dysfunction

  Elizabeth England, et al., bioRxiv - Immunology 2023

  Quote: ... Samples were analysed using the human IL-33 duoset (R&D Systems) and the IL33004-IL330425-biotin assays (AstraZeneca) ...
• Primary lung fibroblasts respond to IL-33, IL-13, and IL-17A by secreting factors that activate macrophages

  Jarrett Venezia, et al., bioRxiv - Immunology 2023

  Quote: Commercially available kits and manufacturer protocols from R&D Systems (mCsf2; DY416) were employed for ELISA ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... recombinant human CCL2 (100 ng/ml, 279-MC-010; R&D Systems) was added to the cells immediately before loading them into the flow chamber and the CCL2 concentration was maintained during the initial 4 min of the assay.
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... were coated with fibronectin (50 μg/ml, 1918-FN; R&D Systems) in PBS and HUVECs were plated at confluence and were stimulated with 40 ng/ml recombinant human TNF-α (210-TA ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... Concentrations of chemokines were determined using ELISA kits from R&D Systems (CCL2 ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... and/or 50 ng/ml IFN-γ (285-IF; R&D Systems) for 18–20 h at 37°C in 5% CO2 ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... IL-1β (20 ng/ml 201-LB-005/CF; R&D Systems) and TNF-α (20 ng/ml 210-TA-005/CF ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... IL-23 (20 ng/ml, 1290-IL-010/CF; R&D Systems), IL-1β (20 ng/ml 201-LB-005/CF ...
• Spatial expression of an mRNA encoding Tie2-agonist in the capillary endothelium of the lung prevents pulmonary vascular leakage

  Katrin Radloff, et al., bioRxiv - Molecular Biology 2022

  Quote: ... 10 % MV2 growth medium with TNFα (R&D Systems, cat. #210-TA) or vehicle was spiked into each well for a final concentration of 10 ng/ml for a further 6 h ...
• A common human MLKL polymorphism confers resistance to negative regulation by phosphorylation

  Sarah E. Garnish, et al., bioRxiv - Immunology 2023

  Quote: ... and 30 ng/ml mouse IFNβ (R&D Systems #8234-MB-010)
• Loss of polarity regulators initiates gasdermin E mediated pyroptosis in human maternal fetal interface trophoblasts

  Khushali Patel, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and IL-1β ELISA kit (DY401-05, R&D Systems Minneapolis MN). Plates were read using the Biotek Synergy HTX plate reader (Gen 5 software) ...
• Stricturing Crohn’s disease single-cell RNA sequencing reveals fibroblast heterogeneity and intercellular interactions

  Pranab K. Mukherjee, et al., bioRxiv - Immunology 2023

  Quote: ... recombinant human CDH11-Fc chimera (CDH11Fc, R&D Systems; Cat# 1970-CA) or H1M1 antibody (prepared from hybridoma PTA-9699 (ATCC ...
• Mammalian Esophageal Stratified Tissue Homeostasis is Maintained Distinctively by the Epithelial Pluripotent p63+Sox2+ and p63-Sox2+ Cell Populations

  Xiaohong Yu, et al., bioRxiv - Cell Biology 2023

  Quote: ... which were containing advanced DMEM/F12 with R-spondin1 (R&D Systems), Noggin (Pepro Tech ...
• Memory Th1 cells modulate heterologous diseases through innate function

  NIkolas Rakebrandt, et al., bioRxiv - Immunology 2023

  Quote: ... together with 20 µg anti-IL18R antibody (clone 112624; R&D Systems) or 60 µg of anti-IL-12p40 antibody (clone C17.8 ...
• An Antibody-Based Molecular Switch for Continuous Biosensing

  Ian A.P. Thompson, et al., bioRxiv - Bioengineering 2023

  Quote: ... was obtained from Sigma Aldrich and digoxigenin monoclonal antibody (Clone # 611621) was obtained from R&D Systems. T4 Ligase and low molecular-weight DNA ladder were obtained from New England Biolabs (NEB) ...
• The lymphatic system favours survival of a unique T. brucei population, and its invasion results in major host pathology

  Henrique Machado, Mariana De Niz, bioRxiv - Microbiology 2023

  Quote: ... we used the antibodies MAB2125 (R&D systems, 20 μg per mouse), and Mouse IgG2 isotype controls were used as controls (eBioscience) ...
• SARS-CoV-2 Mac1 is required for IFN antagonism and efficient virus replication in mice

  Yousef M. Alhammad, et al., bioRxiv - Microbiology 2023

  Quote: ... Human IFN-/β and IFN-γ were purchased from R&D Systems. Cells were transfected with either Polyjet (Amgen ...
• p53 promotes revival stem cells in the regenerating intestine after severe radiation injury

  Clara Morral, et al., bioRxiv - Cell Biology 2023

  Quote: ... and 300 ng/mL human R-spondin1 (R&D Systems #4645-RS).
• A Virus-like particle-based bivalent PCSK9 vaccine lowers LDL-cholesterol levels in Non-Human Primates

  Alexandra Fowler, et al., bioRxiv - Immunology 2023

  Quote: ... using either PCSK9 peptides or recombinant mPCSK9/hPCSK9 protein (R&D Systems) as the coating antigen ...
• Pathogenic bacteria experience pervasive RNA polymerase backtracking during infection

  Kaitlyn R. Browning, Houra Merrikh, bioRxiv - Biochemistry 2023

  Quote: ... supplemented with 10% heat inactivated fetal bovine serum (FBS, R&D Systems), 4 mM L-glutamine ...
• A self-renewing biomimetic skeletal muscle construct engineered using induced myogenic progenitor cells

  Inseon Kim, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-Human/Mouse/Rat/Chicken Pax7 (5μg/ml, R&D Systems, MAB1675), anti-Human/Mouse MyoD (5.8A ...
• Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastoma

  Lise Finotto, et al., bioRxiv - Cancer Biology 2023

  Quote: ... supplemented with 50 ng/ml GM-CSF (Recombinant human, R&D Systems), 10% heat-inactivated FBS (Gibco) ...
• Anopheles salivary apyrase regulates blood meal hemostasis and drives malaria parasite transmission

  Zarna Rajeshkumar Pala, et al., bioRxiv - Microbiology 2023

  Quote: A malachite green assay kit (R&D Systems, Minneapolis, MN, USA, #DY996) was used to determine the phosphate released by rAg5’NTE using AMP (Millipore-Sigma ...
• Disrupting the interaction between AMBRA1 and DLC1 is a promising therapeutic strategy for neurodegeneration that prevents apoptosis while enhancing autophagy and mitophagy

  Kate Hawkins, et al., bioRxiv - Cell Biology 2023

  Quote: Mouse neural stem cells (mNSCs) were purchased from R&D systems (NSC002). The cells were grown in proliferative conditions in complete basal media ...
• NOTCH3 signalling controls human trophoblast stem cell expansion and differentiation

  Bianca Dietrich, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 50 ng/ml recombinant human epidermal growth factor (rhEGF; R&D Systems), 2 µM CHIR99021 (Tocris ...
• Differentiation of Wharton’s Jelly-derived mesenchymal stem cells into insulin-producing beta cells with the enhanced functional level on electrospun PRP-PVP-PCL/PCL nanofibers scaffold

  Seyed Mohammad Javad Hashemi, et al., bioRxiv - Bioengineering 2023

  Quote: ... After adding 10 µl FITC-conjugated anti-insulin antibody (R&D system), incubation was done at RT and away from light for one hour ...
• Pharmacologic Targeting of PDIA1 Inhibits NLRP3 Inflammasome Assembly and Activation

  Jessica D. Rosarda, et al., bioRxiv - Cell Biology 2023

  Quote: ... The Human IL-1β Quantikine ELISA Kit (R&D Systems cat. DLB50) was used to quantify levels of IL-1β according to the manufacturer’s protocol ...
• Plug-and-play protein biosensors using aptamer-regulated in vitro transcription

  Heonjoon Lee, et al., bioRxiv - Bioengineering 2023

  Quote: ... and VEGF 165 (293-VE) were all purchased from R&D Systems Inc ...
• TGF-β3 increases the severity of radiation-induced oral mucositis and salivary gland fibrosis in a mouse model

  Ingunn Hanson, et al., bioRxiv - Biophysics 2023

  Quote: ... injections of 0.1 ml recombinant TGF-β3 (R&D Systems, MN, USA) at a concentration of 2.5 μg/mL ...
• The sleep quality- and myopia-linked PDE11A-Y727C variant impacts neural physiology by reducing catalytic activity and altering subcellular compartmentalization of the enzyme

  Irina Sbornova, et al., bioRxiv - Neuroscience 2023

  Quote: ... supplemented with 10% filtered Fetal Bovine Serum (R&D Systems, Bio-techne) and 1% Penicillin/Streptomycin (GE Healthcare Life Sciences ...
• Time- and lineage-resolved transcriptional profiling uncovers gene expression programs and clonal relationships that underlie human T lineage specification

  Yale S. Michaels, et al., bioRxiv - Immunology 2023

  Quote: ... stem cell factor (SCF) (23.9 ng/ml; R&D Systems, 255-SC), Flt3L (8.7 ng/ml ...
• 14-3-3 binding motif phosphorylation disrupts Hdac4 organized condensates to stimulate cardiac reprogramming

  Liu Liu, et al., bioRxiv - Cell Biology 2023

  Quote: ... and recombinant human FGF basic146 aa (10 ng/ml, R&D Systems) for another 2 weeks as previously described(60).
• Plexin-B1 and Plexin-B2 play non-redundant roles in GABAergic synapse formation

  Susannah S. Adel, et al., bioRxiv - Neuroscience 2023

  Quote: ... conjugated to Fc (R&D Systems, #7470-S4, 4694-S4, 6125-S4) or with Fc (R&D Systems ...
• Geranylgeranyl Isoprenoids and Hepatic Rap1a Regulate Basal and Statin-Induced Expression of PCSK9

  Yating Wang, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Plasma PCSK9 levels were analyzed using an ELISA kit (R&D Systems).