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Citations for R&D Systems :
801 - 850 of 2403 citations for Tumor necrosis factor receptor superfamily member 3 TNFRSF3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and 3 mM CHIR99021 (R&D Systems; 4423). On day 7 ...
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bioRxiv - Bioengineering 2024Quote: ... and 3 mM CHIR99021 (R&D Systems; 4423).
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bioRxiv - Cancer Biology 2019Quote: ... lymph node tissue sections derived from mice bearing pancreatic tumors were incubated overnight at RT with the following primary antibodies: NKp46 (R&D systems AF2225, used at 1:25), Ki67 (Abcam ab15580 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 M/mL PyMT tumor cells were suspended in collagen +/-L-ribose supplemented with basement membrane extract [20 % v/v final (R&D Systems, Cultrex BME, 3532-005-02) and DMEM [1x final] and the pH was adjusted to ∼7.3 ...
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bioRxiv - Microbiology 2021Quote: ... The Hoc proteins each eluted as a single monomeric peak and were run on reducing SDS-PAGE and verified by anti-His western (R&D Systems) (Supplementary Fig.3) ...
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bioRxiv - Developmental Biology 2019Quote: ... A biotin conjugated anti-His-tag antibody (NB100-63172, Bio-Connect) was added followed by a Streptavidin-HRP (DY998, R&D Systems). For a colormetric detection ...
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bioRxiv - Neuroscience 2021Quote: ... The relative expression profile of 36 human cytokines and chemokines were evaluated using the Proteome Profiler Human Cytokine Array Kit (R&D Systems, ON, Canada). The experiment was performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The samples were diluted 1:5 using diluent buffer provided by kit and analyzed by sandwich ELISA for human CCL3 and human CCL4 according to manufacturer’s instructions (R&D Systems, DMB00 and DMA00).
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bioRxiv - Neuroscience 2020Quote: The levels of GDF15 and FGF21 were measured using a Quantikine Human GDF15 ELISA Kit and Quantikine Human FGF21 ELISA Kit (R&D Systems, Minneapolis, MN), respectively.
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Human MΦ were then polarized to a M1 activation state with 30 ng/mL human recombinant IFN-γ (R&D Systems, 285-IF-100) and LPS (100 ng/mL ...
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bioRxiv - Immunology 2023Quote: Serum and recombinant PD-1 was quantified by human PD-1 Quantikine ELISA and human PD-1 DuoSet ELISA (R&D Systems, Minneapolis, MN), following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... was determined using the dot blot-based mouse XL cytokine array kit for the model group breast tissues and human XL cytokine array kit and human XL oncology array kit for the human cell conditioned media following manufacturer’s instructions (R&D Systems, Cat. No: ARY028). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: Human cell line culture supernatants were analyzed using an R&D systems Human Premixed Multi-Analyte Kit (R&D Systems, Inc., Minneapolis, MN, USA) and a Luminex 200 (RRID ...
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bioRxiv - Cancer Biology 2023Quote: ... Cytokine secretion was carried out as a validation from the results of the human cytokine array using a range of human ELISAS from R&D Systems (see below).
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used: monoclonal mouse anti-human CXCR4 (clone 44717) and phycoerythrin-conjugated human CXCR4 (clone 12G5; both from R&D Systems, Minneapolis, MN); purified mouse anti-human CD3 (clone HIT3a ...
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bioRxiv - Molecular Biology 2024Quote: ... CCL2 and TL1A were measured using human CCL2 (MCP-1) and human TL1A ELISA kits by R&D Systems (R&D Systems, Minneapolis, MN). SEMA3C was measured using the human SEMA3C ELISA kit by LS Bio (LS Bio ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured for 96 hours with MOG35-55 peptide (50 μg/mL) and Th17-polarizing factors as follows: recombinant murine (rm)IL-23 (8 ng/ml; R&D Systems), rmIL-1α (10 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... and seeded in RPMI 1640 supplemented with 10% serum and 10 ng/ml recombinant macrophage colony-stimulating factor (M-CSF; R&D Systems) for 3 days ...
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bioRxiv - Immunology 2019Quote: ... from wild type and Irak1−/− mice were prepared by differentiation for 6 days in the same culture medium containing macrophage colony-stimulating factor (M-CSF; 60 ng/mL, R&D Systems). LPS was from Alexis Biochemicals (Salmonella minnesota R595 TLR grade ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were added to the culture plates at the density of 2.0 x 106 cells / dish in a final volume of 10 mL of complete RPMI-1640 supplemented with 20 ng/mL GM-CSF (granulocyte-macrophage colony stimulating factor; R&D Systems). After 3 days ...
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bioRxiv - Bioengineering 2021Quote: ... dickkopf-related protein-1 (Dkk-1) and transforming growth factor beta 1 (TGF-β1) were obtained from R&D systems (Minneapolis, MN) and performed on conditioned media from hMSCs grown on GelMA and OCM-GelMA microcarriers in RWV bioreactors.
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bioRxiv - Bioengineering 2021Quote: ... wells were washed and the growth factors remained bond to the heparin conjugates were detected by incubation with the corresponding antibody (R&D systems) followed by a peroxidase-labeled secondary antibody (Jackson ImmunoResearch) ...
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bioRxiv - Microbiology 2021Quote: ... gradient and treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) respectively for 7 days ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Intestinal organoids were grown in base culture media (Advanced DMEM/F12 media, HEPES, GlutaMax, penicillin, and streptomycin) supplemented with growth factors (EGF, Noggin, R-spondin, R&D Systems), B27 (Life Technologies) ...
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bioRxiv - Genetics 2020Quote: ... femurs and tibias were harvested and bone-marrow cells were obtained by flushing bones and differentiated for 7 days in DMEM media supplemented with 50 mg ml-1 recombinant macrophage-colony stimulating factor (M-CSF, R&D systems), 20% heat-inactivated FBS ...
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bioRxiv - Biochemistry 2021Quote: ... Intestinal organoids were grown in base culture media (Advanced DMEM/F12 media, HEPES, GlutaMax, penicillin, and streptomycin) supplemented with growth factors (EGF, Noggin, R-spondin, R&D Systems), B27 (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... bone morrow cells were cultured in Petri dishes in 10 ml DMEM cell culture medium containing 20 ng/ml of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems). On day 3 of culture ...
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bioRxiv - Microbiology 2021Quote: ... and then treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) for 7 days.
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bioRxiv - Neuroscience 2022Quote: ... either vehicle (PBS) or candidate factors were added at the following concentrations: BDNF (5ng/mL, R&D Systems, Cat# 248-BDB-005), GDNF (5ng/mL ...
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bioRxiv - Microbiology 2022Quote: ... Tissues were minced and digested with collagenase to single cells before embedded in reduced growth factor extracellular matrix (Cultrex BME2, R&D Systems). Selective isolation of ductal stem cells was achieved using Isolation Medium (IM ...
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bioRxiv - Immunology 2022Quote: ... The differentiation to macrophages was initiated at day 0 by the addition of 20 ng/mL macrophage colony-stimulating factor (M-CSF, R&D Systems). Cells were cultured afterwards at 37°C and 5% CO2 for 7 days including the replacement of supplemented DMEM and replenishment of M-CSF at days 1 and 4 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were treated with media containing recombinant mouse transforming growth factor-beta 1 (rmTGFβ1) at 5ng/mL (R&D systems, 7666-MB) or normal media ...
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bioRxiv - Developmental Biology 2023Quote: ... embryonic NSC media was prepared as above without epidermal growth factor and with the addition of 50 ng/mL mouse bone morphogenetic protein 4 (R&D Systems, 5020-BP-010 ...
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bioRxiv - Microbiology 2022Quote: ... were obtained from healthy human blood donors after preparing blood monocyte monolayers and culturing for 2 days in medium containing macrophage colony-stimulating factor (R&D Systems) as described previously (56 ...
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bioRxiv - Systems Biology 2023Quote: ... on plates coated with 10 µg/cm2 Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane (R&D Systems #3434-010-02). Cells were cluster-passaged every 3-4 days with 0.5 mM EDTA and seeded into medium supplemented with 10 µM Y-27632 dihydrochloride (Bio-Techne #1254/10 ...
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bioRxiv - Genetics 2023Quote: ... hiPSCs were plated onto Cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 25 mL flask (cat#353018 Falcon ...
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bioRxiv - Developmental Biology 2023Quote: ... washed 2x with PBS and seeded at a density 50 000 cells/50 μl reduced growth factor BME Type II (CultrexTM, R&D systems) in a 24-well cell culture plate (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... into a 96-well plate pre-coated with non-diluted Cultrex reduced growth factor base membrane extract (RGF-BME) (R&D Systems). The images of the network structure were captured by IncuCyte S3 real-time imager every 2 h at 4X magnification ...
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bioRxiv - Microbiology 2024Quote: ... and were differentiated into monocyte-derived macrophages (MDMs) by stimulation with 20 ng/mL macrophage colony-stimulating factor (M-CSF) (R&D Systems). MDMs were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Cell Biology 2024Quote: ... crypts per isolated from murine intestine and cultured in domes of Cultrex Pathclear Reduced Growth Factor Basement Membrane Extract (3533-005, R&D Systems), covered by ENR media comprised of Advanced DMEM/F12 (12634028 ...
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bioRxiv - Cell Biology 2024Quote: ... a suspension of 25,000 freshly dissociated cells or culture-expanded/FACS-sorted cells ranging from 625 to 5000 cells were prepared in growth factor reduced Matrigel (R&D Systems) and plated in 96-well plates as domes ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-human Gas1 antibodies (R&D systems, Cat# AF2636), mouse anti-Dhh antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems) was used to measure changes in phosphorylation of 43 kinases upon 40 μM Zn2+ treatment ...
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bioRxiv - Immunology 2021Quote: ... or Quantikine Human CXCL8 ELISA kit (R&D Systems, USA) for human MØ ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by recombinant mouse or human Npnt (R&D Systems) at 1.5 μg/cm2 for 2 hours at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... 2 ng/mL recombinant human TGF-β1 (R&D Systems), and 333 ng/mL anti-IgD dextran conjugates (Fina Biosolutions) ...
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bioRxiv - Molecular Biology 2021Quote: ... and human R-Spondin1 (R&D Systems #4645-RS-025) were purchased and used ...
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bioRxiv - Cell Biology 2022Quote: ... or recombinant human WNT5A (0.01 μg/ml, R&D Systems) was supplemented to eMSC cultured in GM ...
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bioRxiv - Cell Biology 2022Quote: Recombinant Human Wnt-3a (R&D Systems 5036-WN-010) was resuspended in in PBS containing 0.1% BSA according to supplied manufacturer recommendations and diluted to 5X concentration in culture medium immediately prior to use ...
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bioRxiv - Genomics 2020Quote: ... 10 ng/mL recombinant human BMP4 (rhBMP4, R&D Systems), and 100 nM retinoic acid (RA ...