Labshake search
Citations for R&D Systems :
801 - 850 of 1173 citations for 7 Chloro 1H pyrrolo 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... and further differentiated into day 3 posterior foregut through the addition of CDM3 base media supplemented with FGF2 (20 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Developmental Biology 2020Quote: ... BMP4 + Activin A treatment was accomplished by treating cells with mTeSR supplemented with 3 ng/mL recombinant human BMP4 protein (R&D Systems) and 100 ng/mL recombinant human Activin A protein (R&D Systems) ...
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bioRxiv - Immunology 2021Quote: ... We used 3 different kits to quantify sCD25 by Human CD25/IL-2R alpha Quantikine ELISA Kit (R&D System, Cat# DR2A00), IP-10 by Human IP- 10 ELISA Kit (CXCL10 ...
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bioRxiv - Developmental Biology 2021Quote: ... and cultured in low cluster plates to allow EB formation in serum-free differentiation SFD media supplemented with BMP4 (3 ng/ml, R&D Systems). At day 1 the medium was changed to SFD media supplemented with Activin A (100 ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... The murine MLL-AF9 AML cells (30) were cultured in the same condition with 10 ng/mL rmIL-3 (R&D systems). Culturing of normal murine HSPCs and human CD34+ UCBs cells has been described previously (31).
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bioRxiv - Cell Biology 2022Quote: ... Rabbit anti-ALIX and rabbit anti-CHMP4B antibodies were described previously (Christ et al, 2016). Human Galectin-3 Alexa Fluor 488-conjugated antibody (cat. no. IC1154G) was purchased from R&D Systems. Mouse anti-LAMP1 (cat ...
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bioRxiv - Molecular Biology 2022Quote: ... iPSC at 60-70% confluence were treated for 3 days with 100 ng/mL Activin A and 100 ng/mL bFGF (R&D Systems) in the presence of increasing levels of FBS (0% on day 1 ...
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bioRxiv - Immunology 2023Quote: ... purified hCD8+ T cells were subjected to 3 consequent cycles of αCD/αCD28 activation in the presence of 50 ng/mL TGFβ (R&D Systems) and 30U/mL rhIL-2 lasting 24h each.
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 million cells (in 100 µL of 1:1 mix of PBS and Cultrex Basement Membrane Extract (Type 3, R&D systems)) were injected into the 4th mammary fat pad of 7-8 weeks old female BALB/c mice ...
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bioRxiv - Cancer Biology 2022Quote: ... or the combination of both drugs in methylcellulose media enriched with human cytokines (recombinant human SCF, GM-CSF, G-CSF, IL-3, IL-6, Epo, #HSC005, R&D Systems). The methylcellulose mixture was plated in three replicates to 35 mm culture dishes using regular syringes with 16 gauge non-stick needles and incubated at 37°C ...
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bioRxiv - Cell Biology 2023Quote: Primary human lung fibroblasts (passages 3-9) were exposed to 5ng/ml recombinant human TGF-β1 (R&D Systems, 240-B-002), 180μM H2O2 (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA or Human CXCL10/IP-10 DuoSet ELISA reagents (biotechne R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Resting or ADP (10 μM)-stimulated platelets (4 × 103/μL) were pretreated with the inhibitor hDcR3 (10 μg/mL, recombinant human decoy receptor 3 protein, R&D Systems) for 10 min and then allowed to adhere to the coated coverslips for 60 min at RT under normoxic or hypoxic conditions ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were passaged by dissociation in 0.5 mM EDTA every 3-4 days and resuspended in Essential 8 Medium supplemented with Y-27632 dihydrochloride (10 μM; Cat.#1254; R&D Systems). For CRISPR-Cas9 editing ...
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bioRxiv - Bioengineering 2024Quote: ... Hydrogels (n=3) were incubated for 4 hours in a solution of FBS-free DMEM supplemented with 10% Resazurin dye (AR002, R&D Systems) at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated over night at 4 °C with the following antibodies diluted in PBS 2.5% BSA: goat anti-CD140a 1:80 (R&D Systems AF1062) and rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S) ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-Human ACE2 (cat.MAB933, R&D System, Minneapolis, MN, USA) diluted 1:33 (15 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... The immunoblots were probed overnight at 4 °C or 1 hour at room temperature with anti-human IL-1β antibody (AF-201-NA, R&D Systems); anti-human Caspase-1 antibody (06-503 ...
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bioRxiv - Immunology 2019Quote: ... and resuspended for culture at 37°C + 5% CO2 for 1 h prior to stimulation with recombinant human TNFα (R&D Systems). Following stimulation ...
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bioRxiv - Neuroscience 2020Quote: ... and 10mM citrate buffer pH 6.0 at 95°C x 5 mins for Prox1 (neutral buffer CTS016, R&D Systems, Minneapolis, MN). Sections were then blocked with 10% or 5% (for Tbr2 ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated with a primary antibody cocktail containing one or more of the following antibodies overnight at 4°C: goat anti-mouse NKp46 (1:100, R&D Systems), anti-mouse CD3e-AF594 or AF647 (500A2 ...
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bioRxiv - Neuroscience 2020Quote: ... the antibody was pretreated with or without 5x recombinant human plexin-B3 (His45-Gln1255 (Gln1156Asp) with a C-terminal 6-His tag) (R&D systems) overnight.
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were blocked with 5% normal donkey serum followed by overnight incubation at 4 °C using the following mixture of primary antibodies: goat anti-IL-1β/ILF2 (1:250; #AF-401-NA, R&D Systems), rat anti-CD45 (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked with blocking buffer for 30 min at room temperature and followed by incubation with primary antibody overnight at 4°C (all primary antibodies used for dot blot were purchased form, R&D systems; anti-Cathepsin A ...
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bioRxiv - Microbiology 2020Quote: Cells were stimulated for 16 h prior to infection in complete medium at 37°C with addition of 50 IU/mL human IFNγ (285-IF, R&D Systems). Induction of Doxycycline-inducible cells was performed with 200 ng/mL Doxycycline overnight (D9891 ...
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bioRxiv - Microbiology 2019Quote: ... BSA (24 - 48 h at 4°C in rotation) for HIS-tagged rPtxS1 and rGαi proteins with mouse monoclonal anti-HIS (1:1000) (R&D Systems, MAB050), for Gαi with mouse monoclonal anti-Gαi (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... the tubuloids were maintained at 37°C and 5% v/v CO2 in Basement Membrane Extract (BME) (R&D Systems, Abingdon, UK) and cultured in expansion medium (ADMEM/F12 supplemented with 1% penicillin/streptomycin ...
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bioRxiv - Microbiology 2022Quote: ... followed by three days incubation at 37 °C in the presence of 1 µg/ml of kifunensine (R&D Systems, MN, USA). Supernatant was collected and combined with half volumes of the supernatant of binding buffer (25 mM HEPES ...
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bioRxiv - Immunology 2020Quote: ... 1.25% of supernatant from Con-A-stimulated rat splenocytes (assay positive control), c) recombinant canine IL-4 (rcaIL-4, 360 ng/mL) (R&D Systems), d ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies were immobilized on CM5-Sepharose chips coated by standard amine coupling chemistry to a density of about 600 response units (RU) Measurements were carried out at 25°C by injecting 250 μl of recombinant human TNFa (R&D Systems) at concentrations from 0.2 to 50 nM with a flow rate of 35μl/min ...
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bioRxiv - Biochemistry 2021Quote: Human CD38 (P28907, a soluble fragment Val43-Ile300 with 6x-His tag at C terminus) was purchased from R&D Systems (USA). It was diluted to final 0.01 mg/ml in 100 mM HEPES/NaOH buffer ...
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bioRxiv - Cell Biology 2022Quote: ... freshly isolated RBCs in basal medium supplemented with 10 mM HEPES at 20% hematocrit were incubated for 30 minutes under 37 °C with gentle shaking with 5 μg/mL ADAMTS13 (R&D Systems) [27 ...
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bioRxiv - Biophysics 2022Quote: ... ECD fragments (modified C-terminally with 10xHis tag) Podocalyxin (PODXL) (Cat.# 1658-PD) and PODXL2 (Cat.# 1524-EG) were obtained from R&D Systems. The ECD fragments were dissolved in ultrapure water to a concentration of 0.5 mg/mL and aliquoted in 50 μL ...
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bioRxiv - Bioengineering 2019Quote: ... Primary antibodies (1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and goat anti-PDX1 (R&D Systems; AF2419) were left on overnight ...
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bioRxiv - Cell Biology 2019Quote: ... slides were blocked and stained using rabbit anti-LOXL2 (Gil25-70, Gilead Sciences) antibody overnight at 4° C and a rabbit cell & tissue staining kit (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested and stocked at −20°C until CXCL8 quantification by ELISA kits according the instructions of the manufacturer (R&D system).
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked in 5% nonfat dry milk in TBST and probed overnight at 4°C with antibodies recognizing ERAP1 (AF2334, R&D Systems), ERAP2 (AF3830 ...
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bioRxiv - Immunology 2020Quote: ... non-tissue culture plates were coated overnight at 4゜C with recombinant human B7-H3 protein (R&D Systems, Minneapolis, MN) at 10 μg/well in 500 μL of PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The pre-immobilized beads were then incubated with 50 nM recombinant mouse SorCS1 ectodomain tagged with a C-terminal 6-His tag (SorCS1-His, Cat# 4395-SR-050, R&D systems) in ECS for 2 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Media for the ELISA was immediately stored after collection at -80°C for subsequent testing in the enzyme-linked immunoassay (R&D systems). The Human IL-1β Quantikine ELISA Kit (R&D Systems cat ...
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bioRxiv - Microbiology 2023Quote: PLY-treated H292 monolayers or PMNs in suspension were treated with 1U PLY and incubated for 30 min at 37 °C with 10 μM fluorogenic peptide MMP substrate (Mca-PLAQAV-Dpa-RSSSR-NH2, R&D Systems) diluted in 25 mM Tris buffer ...
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bioRxiv - Biochemistry 2023Quote: ... plates incubated with biotinylated antibody were washed and incubated at 37°C for 1 hr with a 1/200 dilution of Streptavidin-HRP (Catalog #890803, R&D systems). Plates were then washed and developed using 100□μl/well SureBlue TMB (3,3′ ...
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bioRxiv - Cell Biology 2023Quote: ... were collected 24 h after infection and were kept at − 80 °C until assayed for human IL-6 (DY206-05, R&D Systems) and human IL-8 (DY208-05 ...
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bioRxiv - Immunology 2023Quote: ... The culture was then diluted 1:50 in 1X PBS + 1% LB broth and incubated statically at 37°C with recombinant human SLPI protein (rSLPI) (CAS: 1274-PI-100, R&D Systems) at these final concentrations ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C with the primary antibody (5mC, Active Motif, cat.no. 39649, lot 02617020, used 1:250; SOX2, R&D Systems, AF2018 ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated overnight at 4°C with primary antibodies: sheep anti-THP polyclonal antibody (1:40, catalog no. AF5175; R&D Systems), goat anti-MPO polyclonal antibody (1:200 ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissue was then incubated overnight at 4°C in blocking buffer with anti-E-cadherin (R&D Systems; Minneapolis, MN, USA; cat:MAB1838), anti-integrin-⍺-6 (Stem Cell Technologies ...
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bioRxiv - Biophysics 2024Quote: Recombinant sKLA (Glu34-Ser981) with a C-terminal 6His tag expressed in a NS0 murine myeloma cell line was purchased (R&D Systems) and loaded onto a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Immunology 2021Quote: ... TF-I cells medium contained 2 ng/ml of recombinant human GM-CSF (R&D Systems). Cells have been cultured for no longer than 4 weeks after thawing and were regularly tested for Mycoplasma contamination using PCR technique and were confirmed to be negative.
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bioRxiv - Molecular Biology 2020Quote: ... and TNF-α-treated CM with FGF-2 neutralizing antibody (NAb; AF-233, R&D Systems). On day 7 ...