Labshake search
Citations for R&D Systems :
801 - 850 of 1091 citations for 4 Chloro 2 methoxy N 2 4 methoxy phenyl ethyl benzamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... T cells then were washed and further incubated with recombinant human IL-2 (10 ng/ml; R&D systems) for up to 2 weeks at 37°C incubator with medium change every 3 days ...
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bioRxiv - Cell Biology 2020Quote: ... Autophagy was induced by treating the cells for 2 hours in 250nM Torin-1 (R&D Systems, 4247/10) in 1X Hanks’ Balanced salt solution ...
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bioRxiv - Immunology 2020Quote: ... 2-incubation with 0.5 μg/mL primary anti mouse-TLR4 (recombinant monoclonal rabbit IgG, clone #1203B, R&D Systems) in microscopy buffer for 1 h at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... An ELISA for mouse lipocalin-2 was performed on feces (protocol according to the manufacturer; R&D Systems kit) to determine the inflammatory state of the gut.
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bioRxiv - Bioengineering 2023Quote: ... and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA) were purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Cell Biology 2023Quote: ... and the medium was supplemented with 10 ng/ml rhFGF-2 and 20 ng/ml VEGF (R&D Systems) for EC expansion ...
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bioRxiv - Microbiology 2023Quote: ... 5 fmol or 10 fmol SpyCEP were incubated with 2 pmol human CXCL8 and CXCL1 respectively (R&D Systems), in a final volume of 100 μl and incubated at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... was measured from the resulting supernatant using the Duoset murine Lcn-2 ELISA kit (R&D Systems, Minneapolis, MN). Measuring lipocalin-2 (Lcn-2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then treated with compounds for 2 hours prior to TGF-β1 stimulation (1ng/ml, R&D systems. After 48 hours ...
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bioRxiv - Immunology 2023Quote: ... mouse IFNβ (catalog # 42400-2) cytokine levels were measured using ELISA kits from R&D Systems (Minneapolis, MN, USA). Human IFNβ levels were measured using an ELISA kit (PBL Assay Science ...
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bioRxiv - Neuroscience 2023Quote: ... and the growth factors FGF-2 (20 ng/ml, Alomone Labs) and EGF (20 ng/mL, R&D systems). NSC differentiation was induced withdrawal of these growth factors.
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bioRxiv - Immunology 2022Quote: ... S proteins used in the study were SARS-CoV-2 S GCN4-IZ-2P (10561-CV, R&D Systems), SARS-CoV-2 B.1.1.529 S GCN4-IZ-2P (11061-CV ...
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bioRxiv - Immunology 2023Quote: ... 10 ng/mL of IL-2 and 10 ng/mL of IL-12 (R&D systems, 419-ML-010)) ...
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bioRxiv - Immunology 2023Quote: ... MRT organoids were cultured in droplets of growth factor-reduced basement membrane extract (BME) Type 2 (R&D Systems) in kidney organoid medium (KOM ...
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bioRxiv - Cell Biology 2023Quote: ... starved for 2 hours and treated with 10 ng/ml rat recombinant EGF (R&D Systems, Minneapolis, MN, USA) for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... in 2% BSA/PBS solution and the appropriate fluorescently-conjugated secondary antibody (R&D Systems catalog #NL006, 1:200). Coverslips were mounted onto slide with DAPI-containing ProLong Gold media ...
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bioRxiv - Cell Biology 2023Quote: ... to small clumps and plated on a layer of BME type 2 (R&D Systems, cat. no. 3533-001), incubated overnight in culture medium supplemented with 10 μg/mL polybrene (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2024Quote: ... cells were cultured using recombinant Human IL-2 (R&D Systems, catalog no. 202-GMP or BT-002-GMP) supplemented at 300 IU/mL and stimulated with 12.5 uL/mL Immunocult Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Each microwell was then overlaid with 2 μL of organoid digestion mix including Organoid Harvesting Solution (R&D Systems) with 10 mg/mL B ...
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bioRxiv - Immunology 2024Quote: ... The binding kinetics for the interaction between hmAbs and SARS-CoV-2 Spike protein (R&D Systems, 10549-cv) was determined by injecting two to four concentrations of SARS-CoV-2 RBD (50 nM highest concentration) ...
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bioRxiv - Neuroscience 2021Quote: ... every third sagittal section of tissue containing the left fastigial nucleus was blocked with 5% bovine serum albumin for one hour at room temperature prior to incubation in primary antibody goat anti-osteopontin overnight at 4°C (1:300, R&D Systems AF808; RRID:AB_2194992). Tissue was then rinsed and incubated in donkey anti-goat Alexa Fluor 594 for 2 hours at room temperature (1:500 ...
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bioRxiv - Immunology 2021Quote: ... 115-006-068), 250ng/ml mouse recombinant MEGACD40L (Enzo, ALX-522-120) and 20ng/ml Recombinant mouse IL-4 (R&D system, 404-ML). Cells were collected at specific time points for analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Chromatin was incubated 12-16 hours at 4°C with 10μg of antibody (α-RNA Pol2 [sera from Gardini lab], α-Twist [Abcam ab50887], α-Snail [R&D Systems AF3639] ...
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bioRxiv - Immunology 2020Quote: Macrophages were centrifuged for 7 minutes at 1200 rpm and then stained for 40 minutes at 4°C with fluor ophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation with the appropriate primary antibodies in blocking solution at 4°C overnight (1:1000 rabbit anti-IBA1 (FujiFilm Wako Chemicals, Cat # 019-19741) & 1:500 sheep anti-TREM2 (R&D Systems, Cat # AF1729)) ...
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bioRxiv - Pathology 2020Quote: ... SR-AI and CD-36 proteins were pulled down from MΦ membrane extracts as described above for TLR-4 using anti-SR-A (R&D System, 1:1000) and anti-CD36 (R&D System ...
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bioRxiv - Bioengineering 2020Quote: ... The slides were then incubated overnight in the dark at 4°C with primary antibodies against: Nephrin (NPHS1,AF4269, R&D Systems, 1:300), Lotus Tetragonolobud Lectin (LTL ...
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bioRxiv - Immunology 2020Quote: ... cells were also incubated overnight at 4°C with a sheep anti-Lamp1 Alexa Fluor 488-conjugated (lysosomal marker) antibody (R&D systems, Cat# IC7985G) at 1:10 or a rabbit anti-CD71 (transferrin receptor ...
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 (1 μg/ml) R&D Systems Cat# MAB204) and anti-IFN-γ (1 μg/ml R&D Systems Cat#MAB-285). Differentiation of Th17 cells was confirmed by measuring IL-17 expression by quantitative real-time PCR ...
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bioRxiv - Immunology 2020Quote: ... and IL-10 were measured in the cell-free supernatants of control and treated MoDCs cultures (4×105 cells/ml) by ELISA assays (R&D Systems, Minneapolis, USA). The levels of cytokines produced by CD4+ T lymphocytes in co-cultures with MoDCs were evaluated in the cell-free supernatants of co-cultures ...
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bioRxiv - Cell Biology 2023Quote: ... Then lysates were utilized exactly according to the instructions in the Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA Kit (R&D systems, DYC2724-5).
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bioRxiv - Molecular Biology 2022Quote: ... samples were incubated at 4°C overnight with the respective primary antibodies (LDLR, R&D Systems, 1:200 and CAP1, Santa Cruz, 1:1,000). Cells were then washed with PBS and incubated with the appropriate fluorescent secondary antibody (all from Thermo Fisher ...
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bioRxiv - Systems Biology 2024Quote: ... incubated overnight at 4°C in PBST with the following primary antibodies: mouse SHH supernatant (DSHB, 1:70) and sheep ARX (R&D systems, 1:100), washed in PBST ...
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bioRxiv - Cell Biology 2022Quote: ... MC3T3-E1 Subclone 4 preosteoblasts (ATCC CRL-2593) grown in monolayer were treated with vehicle or CXCL12 (20 μg/ml, R&D Systems, 460-SD) for 4 hours ...
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bioRxiv - Immunology 2022Quote: ... were cultured in OptiMEM reduced serum media buffered with sodium bicarbonate and supplemented with 4% (v/v) fetal bovine serum (FBS, R&D Systems, Minneapolis, MN). The cells were grown at 37 °C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Immunology 2024Quote: ... for 24 hours without or with additional stimulation with a cocktail of 600 U/ml IFNα4 (recombinant mouse IFN alpha 4, R&D Systems, catalog: 12115-1) and 2.5 ng/ml IFNβ1 (recombinant mouse IFN-β1 (carrier-free) ...
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bioRxiv - Immunology 2024Quote: ... BAL was collected and pooled from groups of 5 mice and cultured for 6 hours at 370 with 5% CO2 in the presence of mouse recombinant IL-4 (R&D systems, 404-ML) and IL-10 (R&D Systems 417-ML ...
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bioRxiv - Immunology 2024Quote: ... and the adherent irradiated 40LB cells were co-cultured with 5 × 104 naïve splenic B cells in 6 mL of B cell media containing IL-4 (R&D Systems, 1 ng/mL) for 96 hours ...
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bioRxiv - Neuroscience 2020Quote: ... while MABs differentiation into myotubes was initiated in the opposite compartments using MAB differentiation medium containing 2% horse serum (Cat N° 16050122) and 1% sodium pyruvate in DMEM/F12 supplemented with 0.01 μg/ml recombinant human agrin protein (R&D Systems, Cat N° 6624-AG-050). At day 21 ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-EphA4 N-terminus (R&D Systems, UK; 1:500), goat anti-EphB2 ...
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bioRxiv - Immunology 2021Quote: ... or the N-terminal domain of human ANGPTL4 (R&D Systems) as the target antigens ...
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bioRxiv - Neuroscience 2022Quote: ... N-Terminus (rmShh) (464-SH-025, R&D system, CA, USA) was diluted in PBS 1x and 0,1% BSA to a stock concentration of 125 µg/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were treated for 2 days (days 6, 7) with 50 ng/ml sonic hedgehog (SHH; R&D Systems), 2 μM dorsomorphin (DSM ...
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bioRxiv - Immunology 2021Quote: ... 2×106 cells were kept in 1.5ml Eppendorf tube and stimulated by adding 50nM CCL19 (R&D System 440-M3) and 50nM CCL21 (R&D System 457-6C ...
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bioRxiv - Neuroscience 2020Quote: ... The growth medium was replaced with DMEM supplemented with 2 ng/mL murine M-CSF (R&D Systems; Minneapolis, MN) and cultured in Nx for 3 or 6 days prior to LPS treatment.
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bioRxiv - Immunology 2022Quote: ... cells were washed at day 7 and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 250 U/ml IFN-α (R&D Systems) ...
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bioRxiv - Developmental Biology 2022Quote: ... For the 2-day protocol in which cells were switched from Wnt3a to Activin A (R&D Systems, Minneapolis, MN), the samples were washed with PBS +/+ before adding fresh medium ...
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bioRxiv - Immunology 2022Quote: ... activated B cells were washed and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 5 ng/ml IL-4 (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... organoids were grown embedded in a mix of 90% ice-cold RGF BME Type 2 PathClear (Cultrex, R&D Systems) and 10% Dulbecco’s modified Eagle’s Medium/Ham’s F12 (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... culture supernatants were analyzed for antibody production as well as IL-2 and IFNγ secretion by ELISA (R&D Systems). In CD4 blocking expriments ...