Labshake search
Citations for R&D Systems :
801 - 850 of 1070 citations for 3 4 Fluorophenyl 5 fluorobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Human IL-29/IL-28B (IFN-lambda 1/3) concentration in SARS-CoV-2 infected epithelium basal media was quantified using ELISA technics following manufacturer’s recommendations (R&D systems, Minneapolis, USA). 100 μl of media was used for each point.
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bioRxiv - Neuroscience 2020Quote: Caspase 3/7 activity was measured using a chromogenic DEVD cleavage assay according to manufacturer’s instructions (R&D Systems, Minneapolis, MN, #K106-100).
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bioRxiv - Cell Biology 2021Quote: ... HTM cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments for 3 d: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA), the ERK inhibitor U0126 (10 μM ...
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bioRxiv - Neuroscience 2022Quote: Day 14 MGL media samples collected after 3 and 24h of IL-6 exposure and pooled from one donor over 3 harvests were incubated with Proteome Profiler Human Cytokine antibody array membranes (R&D Systems; ARY005B), as per the manufacturers instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (R&D Systems, #ARY009), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... The following primary antibodies were used: goat anti-Human/Mouse Caspase-3 (R&D Systems, Cat# AF-605-SP, 1:100-1:200), guinea pig anti-CNP1 (Synaptic Systems ...
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bioRxiv - Cancer Biology 2020Quote: ... CTLA-4 and TNF-α levels were quantified by using the commercially available immunoassay kits from R&D systems (Minneapolis, USA) and following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2020Quote: ... and the lysate (100 μg protein) or 4 μg of recombinant human BACE1 (Cat. # 931-AS; R&D Systems, Minneapolis, MN) in extraction buffer was incubated at 37°C for 1 h with a secretase-specific peptide conjugated to two reporter molecules ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibody incubation was done overnight at 4 °C with following dilutions: anti-PLOD2 mouse at 1:500-1:1,000 (MAB4445, R&D Systems), anti-FERMT3 mouse at 1:500-1:1,500 (ab173416 ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: Cells were fixed with 4% paraformaldehyde followed by permeabilization and staining with primary antibodies for LOXL2 (1:500, R&D Systems) and Tetramethylrhodamine (TRITC)-conjugated Phalloidin (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... embryoid bodies were plated on Matrigel (0.17mg/mL in DMEM/F12) in NIM with 10uM BMP-4 (R&D Systems #314BP010). At day 16 ...
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bioRxiv - Pathology 2020Quote: ... 4°C) and plasma was analyzed for TNF-α and IL-10 (porcine specific ELISA kit, R&D Systems, Abingdon, UK). For qPCR ...
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bioRxiv - Immunology 2020Quote: ... 511 or 111 coated filters and stimulated for 4 h with 5nM tumor necrosis factor-α (TNF-α; R&D Systems), then human monocytes or monocyte Hoxb8 cells were added ...
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bioRxiv - Molecular Biology 2021Quote: ... and plated at a concentration of 0.5×106 cells/mL in medium containing 50 ng/mL mouse IL-4 (R&D Systems) and 25 μg/mL lipopolysaccharide (LPS ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the tissue sections were incubated overnight at 4°C with antibodies against myeloperoxidase (1:150 dilution; R&D Systems, AF3667), citrullinated histone H3 antibody (1:100 dilution ...
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bioRxiv - Immunology 2020Quote: ... B cells were labelled with 1μM DDAO (Thermo) and cultured in full RPMI supplemented with 4 μg/ml CD40L (R&D systems), 100ng/ml BAFF (Peprotech) ...
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bioRxiv - Systems Biology 2022Quote: ... membranes were then probed with anti-HSD17β1 polyclonal antibody (Abnova, H00003292-M03A, 1:1000 dilution, overnight 4°C; R&D systems, MAB7178 ...
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bioRxiv - Pathology 2023Quote: ... Tissue sections were labeled overnight at 4°C in a humid chamber with goat anti-mouse CLEC4F (AF2784, R&D systems) and rat anti-mouse/human CD324 (DECMA-1 ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured for 4 days in the presence of 20ng/ml murine recombinant IL-12 (R&D systems; 419-ML). The proportion of IFNγ-secreting CD4+ T-cells (Th1 cells ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated overnight at 4°C with one of the following antibodies: FGE (R&D Systems, MAB2779, 1:1000), LAMP1 (DSHB ...
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bioRxiv - Immunology 2024Quote: ... U-bottom 96 well plates in Essential 8 medium supplemented with 50 ng/mL BMP-4 (R&D Systems, 314-BP010) and 10 μM Rock inhibitor ...
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bioRxiv - Cancer Biology 2024Quote: ... blocking in 10% donkey serum was followed by overnight incubation at 4°C with anti-CD31 (1:100; R&D Systems) and subsequent incubation with a donkey anti-goat AlexFluor594 secondary antibody (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... medium was changed to experimental medium (Ham’s F-12 supplemented with 4% HS and 1X penicillin/streptomycin [R&D Systems; B21210]) for 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... the cells undergoing differentiation were cultured in basal medium supplemented with 5% v/v Osteogenic Supplement (R&D Systems) with the control cells cultured in basal medium ...
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bioRxiv - Immunology 2020Quote: ... and 1 μg/ml CD40-ligand-HA with 5 ng/ml anti-HA antibodies (R&D Systems, Minneapolis, USA), and separately in combination with IL-4 and IL-21 (100 IU each) ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 were maintained in Dulbecco’s Modified Eagle’s medium (DMEM) containing 5% fetal bovine serum (FBS; R&D Systems), gentamicin (50 μg/mL) ...
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bioRxiv - Immunology 2020Quote: Samples stored at −20C were analyzed using IL-5 and IL-13 ELISA kits (R&D Systems, Minneapolis, MN) as per the company’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cytokine protein levels were measured by ELISA (IL-17E, IL-5, and IL-13 Duo-Set, R&D Systems). Cytokine levels were normalized to total protein concentration in the tissue lysates using the DC Protein Assay (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... 5 fmol or 10 fmol SpyCEP were incubated with 2 pmol human CXCL8 and CXCL1 respectively (R&D Systems), in a final volume of 100 μl and incubated at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... from Sino Biological (10247-HNAB) or PTN (5 ng/µl equivalent to 327 nM) from R&D Systems (R&D252-PL-050). The final volume within each well was 100 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteasome activity was also measured using 5 nM of recombinant human 26S proteasome (Cat: E-365; R&D Systems).
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bioRxiv - Biophysics 2020Quote: ... cells were changed to long term DMEM/F12 media50 in the presence or absence of 3 ng/ml TGF-β1 (R&D Systems, Abingdon, UK). Media was replenished three times per week ...
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bioRxiv - Microbiology 2021Quote: ... S1-Fc (Catalog # 10623-CV, lot DONK0120091) and S1-biotin (Catalog # BT10569, lot DOJW120092) were purchased from R&D Systems (Vendor #3; Minneapolis, MN). Streptavidin (Catalog# 85878-10MG ...
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bioRxiv - Pathology 2022Quote: ... BMMs were plated into 96-well or 24-well plates (3 × 104 cells per milliliter) in complete α-MEM culture media supplemented with 50 ng/mL RANKL (R&D Systems, USA) and 25 ng/mL M-CSF (PeproTech ...
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bioRxiv - Neuroscience 2023Quote: ... On days 27 to 40 organoids were fed on alternate days with media containing 20 ng/mL NT-3 (R&D Systems, 267-N3) and 10 ng/mL BDNF (R&D Systems 248-BD) ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.3% Triton X-100) for 1 hour at room temperature and incubated with primary antibody against VE-cadherin (AF938, R&D Systems, 1:100) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 3 uM CHIR, 300 nM LDN193189 (LDN, Axon, Scottsdale, AZ) and 100 ng/mL epidermal growth factor (EGF, R&D systems, Minneapolis, MN) in Advanced DMEM/F12 (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... Stem cells underwent directed differentiation into definitive endoderm over a 3-day treatment using Activin A (100ng/mL, R&D Systems Cat#338-AC) added to RPMI base media ...
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bioRxiv - Cancer Biology 2021Quote: ... MO) or using 50 μg/ml of LPS plus 50 ng/ml of mouse IL-4 from R&D Systems (Minneapolis, MN). After 4 days in complete RPMI media at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated over night at 4 °C with the following antibodies diluted in PBS 2.5% BSA: goat anti-CD140a 1:80 (R&D Systems AF1062) and rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S) ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-Human ACE2 (cat.MAB933, R&D System, Minneapolis, MN, USA) diluted 1:33 (15 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... The immunoblots were probed overnight at 4 °C or 1 hour at room temperature with anti-human IL-1β antibody (AF-201-NA, R&D Systems); anti-human Caspase-1 antibody (06-503 ...
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bioRxiv - Developmental Biology 2021Quote: ... and genomic DNA regions of interest were isolated using 4 μg of antibody against Brachyury (R&D Systems, cat# AF2085, lot# KQP0719121). Complexes were washed ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated with a primary antibody cocktail containing one or more of the following antibodies overnight at 4°C: goat anti-mouse NKp46 (1:100, R&D Systems), anti-mouse CD3e-AF594 or AF647 (500A2 ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were blocked with 5% normal donkey serum followed by overnight incubation at 4 °C using the following mixture of primary antibodies: goat anti-IL-1β/ILF2 (1:250; #AF-401-NA, R&D Systems), rat anti-CD45 (1:250 ...
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bioRxiv - Bioengineering 2021Quote: ... primary antibodies (E-cadherin, AF748, R&D Systems, 4 μg/mL; vimentin, ab92547, Abcam, 0.5 μg/mL; CD31, AF3628, R&D Systems, 10 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked with blocking buffer for 30 min at room temperature and followed by incubation with primary antibody overnight at 4°C (all primary antibodies used for dot blot were purchased form, R&D systems; anti-Cathepsin A ...
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bioRxiv - Microbiology 2021Quote: ... gradient and treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) respectively for 7 days ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were kept in serum-free medium for 4 hours prior to stimulation and then incubated with recombinant CSF-1 or IL-34 (R&D Systems) for the indicated time points ...
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bioRxiv - Immunology 2022Quote: The supernatants collected from LPS-stimulated moDCs with or without fhFABP1 (25 μg/ml) were pooled from 4 donors in equal volume and subjected to the Proteome Profiler Human XL Cytokine Kit (R&D Systems) according to the manufacturer’s instructions ...