Labshake search
Citations for R&D Systems :
801 - 850 of 975 citations for 10 Methylundecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL heregulin-1β (recombinant human NRG-beta 1/HRG-beta 1 EGF domain; R&D Systems, Minneapolis, MN, USA), 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... were prepared to obtain solutions of TGF-β1 (10 ng/mL and 25 ng/mL, R&D Systems 240-B-002), palmitic acid (PA ...
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bioRxiv - Genomics 2023Quote: ... MDA-MB-231 cells were cultured in DMEM high-glucose medium with 10% FBS (Thermo Fisher Scientific or R&D Systems). Doxycycline-inducible GATA3 expression system in MDA-MB-231 cells was developed by lentiviral transduction ...
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bioRxiv - Immunology 2023Quote: ... cells were pelleted by centrifugation and media was replaced with plasmablast generation media: 10 ng/mL IL-2 (R&D Systems), 50 ng/mL IL-6 (Peprotech) ...
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bioRxiv - Immunology 2023Quote: ... ELISA for human IL-6 and IL-10 were performed using ELISA kits (DuoSet [R&D Systems] or BD OptEIA [BD]) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... IL-10 and CCL7 were measured using a multiplex Luminex bead assay by R&D Systems (R&D Systems, Minneapolis, MN). CCL2 and TL1A were measured using human CCL2 (MCP-1 ...
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bioRxiv - Immunology 2024Quote: ... and 10 μl biotinylated rhIL-2 or biotinylated control protein (from Fluorokine® Biotinylated Human IL-2 Kit, R&D Systems) added to each well ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were fixed for 10 min in ice-cold methanol and incubated with primary antibodies (goat E-cadherin, R&D systems) and nuclear stain (Hoechst ...
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bioRxiv - Microbiology 2024Quote: ... Monocytes were differentiated into hMDMs for at least 7 days in complete medium containing 10 ng.mL-1 of recombinant human macrophage colony-stimulating factor (rhM-CSF; R&D systems).
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in osteoclast medium supplemented with 10 ng/ml of M-CSF and 5 ng/ml of murine RANKL (R&D Systems) for 7-10 days ...
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bioRxiv - Cell Biology 2020Quote: ... Bone marrow cells were plated in four replicates in 24-well plates at a density of 5 × 104 cells/well with 10 ng/ml of M-CSF (R&D Systems). Three days later ...
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bioRxiv - Microbiology 2020Quote: ... Fully-differentiated MDM were generated by culturing MC under adherent conditions for seven days using RPMI-AS medium supplemented with 10 ng/mL M-CSF (R&D Systems). T cells were purified from PBMC using T cell pan isolation kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... We used commercially available ELISA kits to quantify plasma IFNγ (mouse IFNγ platinum ELISA extra sensitive, ebioscience), CXCL10 (mouse IP-10 platinum ELISA, ebioscience) and Angiopoietin-2 (mouse/rat Angiopoietin-2 quantine ELISA kit, R&D Systems). Absorbance was measured using a Multiskan Ascent (MTX Lab systems ...
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bioRxiv - Bioengineering 2022Quote: MONHA-encapsulated hydrogels were cultured in MONHA growth medium for 21 days followed by treatment with increasing doses of TGFβ2 (vehicle control, 2.5 ng/ml, 5 ng/ml, and 10 ng/ml; R&D Systems, Minneapolis, MN) for 7 days ...
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bioRxiv - Immunology 2022Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2019Quote: ... a suspension of 106 mononuclear cells in RPMI 1640 supplemented with 10% FCS was added to each insert in a well containing a solution of chemokine (R&D Systems). In some experiments ...
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bioRxiv - Pathology 2019Quote: Supernatants were tested for 6 analytes (TNFa, IL-6, IL-8, IL-10, IL-1b, IL-1a) on a custom Human magnetic Luminex plate (R&D systems LXSAHM ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were added to the culture plates at the density of 2.0 x 106 cells / dish in a final volume of 10 mL of complete RPMI-1640 supplemented with 20 ng/mL GM-CSF (granulocyte-macrophage colony stimulating factor; R&D Systems). After 3 days ...
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bioRxiv - Bioengineering 2020Quote: ... Media for M(IFN-γ) stimulation consisted of RPMI 1640 supplemented with 10 ng/ml interferon-γ (IFN-γ; R&D systems), and media for M(IL4/IL13 ...
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bioRxiv - Cell Biology 2021Quote: ... PDGFRα homodimer cells and PDGFRβ homodimer cells at ∼60-70% confluence were serum starved for 24 h in HITES media and stimulated with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... A feeding layer of 2 mL RPMI growth media supplemented with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) was added on top of the agar and replaced every day ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were allowed to recover for 5 min and were treated with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) for the indicated amount of time ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were re-suspended in 100 μl of FACS buffer and incubated with 10 μl of PE-conjugated anti-sheep IgG secondary antibody (F0126, R&D systems) for 30 min on ice ...
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bioRxiv - Immunology 2022Quote: ... Culture supernatant from treated cells were collected and applied to the IP-10 antibody-coated plate for ELISA analysis (R&D Systems) following the manufacture’s instruction.
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bioRxiv - Bioengineering 2022Quote: ... Positive control neutrophils were incorporated into MCDB-131 Complete Media at a concentration of 900k cells.mL-1 and supplemented with 10 ng.mL-1 IL-8 (rh CXCL8/IL-8, R&D Systems, Minneapolis, MN) for 15 minutes before rinsing and stained ...
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bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: ... sections were blocked with 10% rabbit serum for 15min at room temperature and then incubated with goat anti mouse IGF1(1:40, AF791, R&D System) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated at 37 °C for 30 minutes with 15 μm Forskolin and 1:3 serial dilutions of potential ligands with the following starting concentrations: 10 μM recombinant human CXCL17 (R&D Systems), 10 μM lysophosphatidic acid (LPA ...
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bioRxiv - Immunology 2022Quote: ... with IFN-γ 100ng/mL and IL-10 100ng/mL in the presence or absence of human anti-Nectin-2 monoclonal antibody 20 μg/mL (R&D Systems) for 6h ...
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bioRxiv - Immunology 2022Quote: ... at a final concentration of 60 pmol/peptide/mL in CTLm supplemented with 400 U/mL IL-4 and 10 ng/mL IL-7 (R&D Systems) for three days ...
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bioRxiv - Neuroscience 2019Quote: ... for 24h or with 10 ng/mL tumor necrosis factor alpha and 10ng/ml IL1-ß(R&D Systems; 401-ML-005) for 24h ...
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bioRxiv - Immunology 2020Quote: ... were generated by incubation of bone marrow cells for 5 days in the presence of 10 ng/ml of recombinant murine GM-CSF and IL-4 (R&D Systems), as described previously [40] ...
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bioRxiv - Immunology 2019Quote: Protein levels in cell-free sputum samples of CXCL10/IP-10 and CCL5/RANTES were measured using duoset ELISA kits (R&D Systems), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteome Profiler Human Apoptosis Array kit (CAT# ARY009) and Pepstatin A aspartic protease inhibitor (#1190/10) were purchased from R&D Systems. The cOmplete protease inhibitor cocktail (CAT# 11697498001 ...
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bioRxiv - Cancer Biology 2020Quote: ... After three PBST washes the dot blots were incubated with high sensitivity streptavidin conjugated to horseradish peroxidase (1:10 dilution, 865006, R&D Systems) for 90 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Dot blots were washed with PBST three times and incubated with 30 μl of biotinylated anti-rabbit secondary antibody for 90 minutes (1:10 dilution, 865002, R&D Systems). After three PBST washes the dot blots were incubated with high sensitivity streptavidin conjugated to horseradish peroxidase (1:10 dilution ...
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bioRxiv - Biochemistry 2020Quote: ... a total of 1 μg native proMMP2 and 0.5 μg proMMP14 were separately incubated in 10 μl with a range of furin (catalog no. 1503-SE-010, R&D systems) concentrations (0 – 250 nM ...
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bioRxiv - Immunology 2021Quote: ... The supernatants were collected 72 hours post-stimulation and the levels of IFNγ and IL-10 determined by and ELISA (R&D Systems). IFNγ production was also assessed by ELISPOT ...
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bioRxiv - Developmental Biology 2021Quote: ... and then further differentiated on days 4–6 with CDM3 base media supplemented with Activin A (10 ng/mL; R&D Systems), BMP4 (30 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IL-10 clone 948505 (MAB9184) and the isotype controls mouse IgG2a (MAB003) and mouse IgG1 (MAB002) (all from R&D systems).
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bioRxiv - Immunology 2020Quote: ... bone morrow cells were cultured in Petri dishes in 10 ml DMEM cell culture medium containing 20 ng/ml of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems). On day 3 of culture ...
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bioRxiv - Immunology 2021Quote: ... one hundred microliter of pre-warmed complete medium containing 10 ng/mL recombinant mouse IL-2 (rmIL-2; 402-ML-020/CF, R&D Systems) or 20 ng/mL rmIL-7 was added to each Nucleocuvette™ strip well for activated or naïve CD8+ T cells ...
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... were blocked using 5μg of mouse serum IgG for 10 min at RT then incubated with mouse anti-human ACE2 (R&D systems, FAB9333G), mouse antihuman CD81 (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2020Quote: ... T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Microbiology 2021Quote: ... and 10μM SB431542 for 24 hours followed by switching to SFD media supplemented with 10 μM SB431542 and 1 μM IWP2 (R&D Systems) for another 24 hours ...
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bioRxiv - Immunology 2021Quote: ... fifty microliters of stimulant pepmixes (JPT peptides) resuspended in RPMI+10%FBS (R10) with recombinant mouse IL-2 (rmIL-2) (R&D Systems) were added to each well ...
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bioRxiv - Cell Biology 2021Quote: Healthy dermal fibroblasts were serum starved for 24 h in DMEM containing 0.5% FBS and stimulated with 10 ng/ml TGF-β (R&D systems), 100 ng/ml Wnt-3a (R&D systems ...