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Citations for R&D Systems :
8201 - 8250 of 10000+ citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The ligands NRP1 (Cat. #3870-N1-025/CF, R&D Systems) and VEGFR2 (Cat ...
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bioRxiv - Bioengineering 2024Quote: ... using human ALB and AFP DuoSet ELISA kits (R&D System).
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bioRxiv - Cell Biology 2024Quote: ... and 10 ng/mL GDNF (R&D Systems, 11166-BD-010). The following day ...
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bioRxiv - Cell Biology 2024Quote: ... 10 ng/ml PDGF-AA Protein (R&D Systems, 221-AA), 10 ng/ml IGF-I Protein (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... and HRP-conjugated anti-rat IgG antibody (HAF005; R&D Systems), anti-mouse-IgG antibody (A90-516P ...
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bioRxiv - Cell Biology 2024Quote: ... mouse EGF (10 ng/ml, R&D Systems, Minneapolis, MN, 2028EG200), and Y-27632 compound (10 μM ...
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bioRxiv - Cell Biology 2024Quote: ... mouse EGF (10 ng/ml, R&D Systems, Minneapolis, MN, 2028EG200), and Y-27632 compound (10 μM ...
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bioRxiv - Cancer Biology 2024Quote: ... biotinylated m-SCF R/c-KIT antibody (R&D Systems BAF1356). Cells were then washed with cold FACS buffer ...
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bioRxiv - Cancer Biology 2024Quote: Commercial purified mouse ephrinB2-Fc was purchased from R&D Systems (#496-EB-200 ...
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bioRxiv - Cell Biology 2024Quote: ... were treated with 45 mg/kg streptozotocin (1621500, R&D Systems) in 0.9% sterile saline (51-405022.052 ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was analyzed using Mouse Cytokine Microarray (R&D Systems). The resulting blot was imaged on ChemiDoc MP Imaging System and analyzed with Image Lab software (both Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... The Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems) was used to analyze the phosphorylation of 37 kinases in Hut78 cells ...
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bioRxiv - Neuroscience 2024Quote: ... and 20 ng/μl EGF (R&D Systems, Cat. #236-EG) and vDM III contained 0.1 μM SAG and 50 ng/μl FGF8 (PeproTech ...
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bioRxiv - Microbiology 2024Quote: ... in flasks coated with 150 µg/mL Cultrex (R&D Systems). All cells were routinely tested for mycoplasma contamination with a PCR-based detection kit either in-house (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... and Alexa Fluor 647 goat anti-GATA6 (R&D System, AF1700). These prelabelled antibodies were prepared in house with Molecular Probes Antibody Labeling Kits (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+for 1h at RT or with negative isotype control mouse IgG2a (cat ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were stained with 1:75 Anti-Hu Osteopontin (SPP1) eFluor 660 (eBioscience; Cat. No. 50-9096-42) or 1:75 Human CCL13 488 (R&D Systems; Cat. No. IC327G) or their isotype controls Mouse IgG1 Control Alexa Fluor 488 conjugated (R&D Systems ...
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bioRxiv - Genomics 2020Quote: ... LDLR staining was performed for 30 min on ice with a 1:50 dilution of AlexaFluor488-conjugated LDLR antibody (R&D Systems, Minneapolois MN, FAB2148G) into PBS supplemented with 1% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and buffers were prepared using the DuoSet ELISA Ancillary Reagent Kit 1 for the TGF-β and mTGF-β ELISAs (R&D Systems, Minneapolis, MN). Standards ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were incubated with the primary antibodies (1:100 dilution; CD68, Bio-Rad, Cat# MCA1957; iNOS, Abcam, Cat# ab15323; CD206, R&D Systems, Cat# AF2535) in TBST containing 2% BSA for 1 h at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... transferred membrane was reacted with first antibodies as follows: anti-mouse and human CHL1 antibody (AF2147 and MAB2126; R&D systems, MN; 1 μg/ml), HRP-conjugated anti-mouse and human CHL1 antibody (described above) ...
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bioRxiv - Cancer Biology 2020Quote: ... CHLA9 and CHLA10 cells were cultured in IMDM +L-glutamine media supplemented with 20% FBS and 1% insulin-selenium-transferrin (ITS, R&D Systems, cat no: AR013). Cell lines were maintained at 37°C and 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IL-1β in conditioned medium was analysed by western blotting on nitrocellulose membrane with a goat anti-mouse IL-1β antibody (AF-401-NA, R&D systems, 1:1000 dilution), donkey anti-goat horseradish peroxidase-conjugated secondary antibody (A15999 ...
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bioRxiv - Cancer Biology 2021Quote: ... the culture media were replaced with 1 ml fresh media supplemented with 10% FBS and the designated concentration of the recombinant mouse IFNγ (R&D Systems, 485-MI-100) or recombinant human IFNγ (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: The following antibodies were used for in vitro biotinylation (dilutions for each antibody are indicated in parentheses): chicken polyclonal antibody anti-neurofascin (1:1000; R&D Systems Cat# AF3235, RRID:AB_10890736), rabbit polyclonal anti-NrCAM (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: The following antibodies were used for immunofluorescence studies (dilutions for each antibody are indicated in parentheses): chicken polyclonal anti-neurofascin (1:500; R&D Systems Cat# AF3235, RRID:AB_10890736), chicken polyclonal anti-MAP2 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Media was replaced with DMEM containing gentamicin and ciprofloxacin HCl alone or containing antibiotics and 10% NCS and/or 200 ng/mL Recombinant Human Insulin growth factor 1 (R&D Systems Cat#: 291-G1) for indicated minpost-treatment ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were washed and blocked in PBS with 2% donkey serum and 2% bovine serum albumin for an hour and incubated with rabbit anti-IBA1 (WAKO, cat#019-19741, RRID:AB_839504, 1:500) and goat anti-GPNMB (R&D Systems, cat#AF2550, RRID:AB_416615, 3µg/ml) primary antibodies in PBS with 2% donkey serum overnight at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... A “type-1” like cytokine cocktail consisted of 10 ng/ml of recombinant mouse tumor necrosis factor-α (TNF-α, R&D Systems, Minneapolis, MN), 5 ng/ml of recombinant mouse interleukin-1β (IL-1β ...
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bioRxiv - Developmental Biology 2023Quote: The following primary antibodies were used at indicated concentrations for the immunostaining of compresstome sections or iDisco whole lobe samples: goat anti-EPCR (R&D Systems, AF2749, 1:100), rat anti-Endomucin (Santa Cruz Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... Medium was then changed to 500 µl OPC base medium (untreated control (=UC)) or OPC base medium supplemented with 1 ug/ml Osteopontin (cat#: 6359-OP, R&D Systems, Minneapolis, MN, USA) (OPN) ...
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bioRxiv - Neuroscience 2023Quote: ... A Nanoject II (Warner Instruments) was used to infuse 1 μl of vehicle (DMSO + saline) or AQ-RA 741 (R&D SYSTEMS, in DMSO + saline) before stimulus presentation on experimental days 1 and 2 under isoflurane anesthesia ...
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bioRxiv - Synthetic Biology 2023Quote: ... PD-1-DNR interaction with PD-L1 was assessed by staining with 1 μg Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein (R&D Systems, 1019-B7-100), followed by anti-hFc antibody.
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bioRxiv - Bioengineering 2022Quote: ... and Alexa Fluor 647-conjugated Human NCAM-1/CD56 (MC-480) live stain (catalog #FAB24081R-100UG) were purchased from R&D Systems (Minneapolis, MN, USA) as well as Human Three Germ Layer 3-Color Immunocytochemistry Kit (catalog #SC022 ...
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bioRxiv - Cancer Biology 2023Quote: ... and the signalling ligands identified from the ligand-receptor analysis (Figure 4C): murine WNT5A (250 ng mL−1, R&D Systems 645-WN-010/CF), murine SEMA3A (250 ng mL−1 ...
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bioRxiv - Developmental Biology 2023Quote: Flow cytometry performed within 1-6 days investigated NOTCH1 expression using anti-NECD1 antibody AF5267 and Notch ligand binding for DLL1-Fc (R&D Systems, Inc., Minneapolis, MN) and DLL4-Fc (Aro Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FBS and 1% penicillin/streptomycin with macrophage-colony stimulating factor (M-CSF, 30 ng/mL; 416-ML, R&D systems, Minneapolis, MN, USA) for 4 days to obtain BMMs ...
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bioRxiv - Synthetic Biology 2023Quote: ... EV samples at a concentration of 1 × 1010 particles/mL were incubated with AF647-labelled anti-TSPAN2 antibody (R&D systems, FAB7876R-100UG, 0.5 µL) or a mixture of APC-labelled anti-CD9 (Miltenyi Biotech ...
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bioRxiv - Immunology 2024Quote: Caspase-1 levels in macaque serum samples were quantitatively determined using the Quantikine™ ELISA Human Caspase-1/ICE Immunoassay (Catalog# DCA100, R&D Systems, Minneapolis, MN). The assay was conducted following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Cell culture plates for cell invasion assays were prepared by adding 150 μL of 1 mg/mL Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems, 3433-005-R1) to a 24-well cell culture insert with an 8 μm pore size (Falcon ...
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bioRxiv - Neuroscience 2024Quote: Animals were randomly assigned to experimental groups and injected intrathecally with saline or CCL2 (1 µg/rat, dissolved in 0.1% BSA in sterile saline; R&D Systems #3144-JE-025/CF) on postoperative days (PODs ...
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bioRxiv - Bioengineering 2024Quote: ... the primary antibody was washed off with PBS and secondary antibody (NorthernLights™ Anti-goat IgG-NL637 Cat: NL002; R&D Systems, 1:50 dilution) was added to tissue sections and incubated for 2 hours at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1×107 cells and 0.1 μL of each antibody (V5 tag antibody, Invitrogen 451098; His tag antibody, Cell Signaling Technology 14930; or Myc tag antibody, R&D Systems IC3696P) were incubated (for 45 min ...
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bioRxiv - Developmental Biology 2024Quote: ... the embryoid bodies (EB) were harvested and seeded in serum-free differentiation media supplemented with 1 ng/ml human Activin A (R&D Systems Cat#. 338-AC) and 1 ng/ml human BMP4 (R&D Systems Cat# 314-BP) ...
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bioRxiv - Neuroscience 2020Quote: ... Cytokine release was assessed using magnetic multiplex assay (Rat Magnetic Luminex® Assay, Rat Premixed Multi-Analyte Kit, R&D Systems, Bio-Techne, R&D Systems Inc., USA) on a Bio-Plex 200 station (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... Cytokine release was assessed using magnetic multiplex assay (Rat Magnetic Luminex® Assay, Rat Premixed Multi-Analyte Kit, R&D Systems, Bio-Techne, R&D Systems Inc., USA) on a Bio-Plex 200 station (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were pre-treated for 1 day with vehicle control (0.1% BSA in 1X PBS) or 400 ng/ml human recombinant IL-1RA (R&D Systems, Minneapolis, MN; 280-RA/CF) 0 in the DMEM/10% FBE growth medium ...
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bioRxiv - Neuroscience 2020Quote: ... while MABs differentiation into myotubes was initiated in the opposite compartments using MAB differentiation medium containing 2% horse serum (Cat N° 16050122) and 1% sodium pyruvate in DMEM/F12 supplemented with 0.01 μg/ml recombinant human agrin protein (R&D Systems, Cat N° 6624-AG-050). At day 21 ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Gi-bound complex sample) or mixed with 0.5 mg/mL CXCL12 (Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha, R&D Systems Catalog #: 350-NS-050/CF), or 1 mM AMD3100 (AMD 3100 octahydrochloride ...