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Citations for R&D Systems :
751 - 800 of 851 citations for Dengue Virus Serotype 3 DIII envelope protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... GPC2 CAR expression was determined using APC or PE-conjugated human recombinant GPC2 protein (R&D Systems; 2304-GP-050; 1:500) and CD19 CAR using PE-tagged protein L (58036S ...
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bioRxiv - Neuroscience 2024Quote: ... Relative expression levels of 105 soluble human proteins and cytokines were determined using the Human XL Cytokine Array Kit from R&D Systems. The cytokine array was performed following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... after which the organoids were cultured for 13 days in E6 supplemented with 50 ng/mL bone morphogenetic protein-7 (BMP7, R&D systems) and 10 ng/mL human epidermal growth factor (hEGF ...
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bioRxiv - Immunology 2024Quote: ... 1 mg of pre-cleared lysates was incubated with 50 µL Dynabeads Protein G (30 mg/ml) and 5 µg Regnase-1 (R&D systems) or Regnase-3 (Helmholtz Munich Monoclonal Antibody Core Facility ...
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bioRxiv - Immunology 2023Quote: ... The culture was then diluted 1:50 in 1X PBS + 1% LB broth and incubated statically at 37°C with recombinant human SLPI protein (rSLPI) (CAS: 1274-PI-100, R&D Systems) at these final concentrations ...
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bioRxiv - Neuroscience 2023Quote: ... co-cultures were maintained for 24 hours in the control condition or in the presence of the chimera proteins Fc-LGR4 (8077-GP, R&D Systems), Fc-LGR5 (8078-GP ...
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bioRxiv - Microbiology 2022Quote: ... IL-6 protein was detected by Hamster IL-6 Sandwich ELISA Kit (AssayGenie) or Mouse IL-6 Duoset Sandwich ELISA kit (R&D Systems) according to manufacturers’ instructions ...
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bioRxiv - Neuroscience 2022Quote: ... VEGFR-1 and VEGFR-2 protein levels were quantified with the DuoSet® ELISA Development System Kit (R&D Systems, Abingdon, UK), according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and/or 1 μmol/L ribociclib for 48 hours and then 500 μg protein lysates were assessed using human apoptosis antibody array (#ARY009, R&D systems).
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bioRxiv - Immunology 2023Quote: ... KIR2DL3*001 (#2014-KR-050) and KIR2DS4*001 (#1847-KR-050) KIR-Ig (KIR-Fc) fusion proteins were purchased from R&D systems. KIR2DS1*001-Fc was custom produced by Sino Biological ...
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bioRxiv - Developmental Biology 2023Quote: ... embryonic NSC media was prepared as above without epidermal growth factor and with the addition of 50 ng/mL mouse bone morphogenetic protein 4 (R&D Systems, 5020-BP-010 ...
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bioRxiv - Immunology 2023Quote: ... Levels of BAFF protein were determined in culture supernatant or bone marrow plasma using the human BAFF/BLyS/TNFSF13B DuoSet ELISA kit (R&D Systems) in combination with the Pierce Streptavidin-Poly-HRP (1:10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... and B27 were omitted and lt-NES cells were cultured at low density in a differentiation-defined medium (DDM) in the presence of bone morphogenetic protein 4 (BMP4) (10 ng/mL, R&D Systems), wingless-type MMTV integration site family ...
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bioRxiv - Cancer Biology 2023Quote: ... The amount of CXCL1 or CXCL2 protein in the supernatant was determined using a mouse CXCL1 or CXCL2 specific ELISA kit (R&D system). All experiments were performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... For activation endothelial cells were incubated with hMDM conditioned media for 6 h in absence or presence of TNFRII/TNFRSF1B-Fc chimera protein (726-R2-050, R&D Systems) at 1 µg/ml.
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bioRxiv - Immunology 2024Quote: ... In vitro TNFα-blocking studies were performed using recombinant human TNFRII/TNFRSF1B-Fc chimera protein (1 µg / ml) (726-R2-050, R&D Systems) for 18 h.
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bioRxiv - Microbiology 2024Quote: ... or N protein of SARS-CoV-2 (dilution 1:2000) or SARS-CoV-2 membrane ab (R&D Systems, Cat no. MAB10690). Followed 3 time washing with PBST (PBS with 0.1 tween 20 ...
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bioRxiv - Immunology 2024Quote: Calu-3 or A549 cells were treated with 10 or 100 ng/mL of recombinant human IL-29/IFN-lambda 1 Protein (R&D Systems) for 6 or 24 hours at 37°C ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... and acute phase proteins (total 36) present in the supernatants were detected with using Proteome Profiler Human Cytokines Array Kit (R&D Systems) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... IL-1β protein levels were assessed using the Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN) following the manufacturer’s directions ...
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bioRxiv - Systems Biology 2024Quote: ... Intestinal and/or serum levels of several other proteins were measured using a custom Mouse Pre-Mixed Multi-Analyte Kit (R&D Systems; CHI3L1 ...
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bioRxiv - Systems Biology 2024Quote: ... before being incubated with serum-free DMEM with Recombinant Mouse GAS6 protein (0.05– 1000ng/mL, R&D Systems, 986-GS-025/CF), Recombinant Mouse Protein S/PROS1 (100ng/mL ...
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bioRxiv - Cell Biology 2024Quote: ... were O/N coated at 4°C with 5 µg/ml Invasin protein in PBS or 2% BME® (R&D system) diluted in PBS ...
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bioRxiv - Developmental Biology 2024Quote: The expression of 111 proteins of interest was semi-quantitatively measured in pooled decidual lysates using the Mouse XL Cytokine Proteome Profiler Array (R&D Systems). Tissue lysates were thawed and clarified by centrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... cultures were cotreated with 20 nM (2S,3S)-2-Amino-3-methyl-N-[2-(4-morpholinyl)ethyl]pentanamide dihydrochloride (LM11a-31)(R&D Systems, Minneapolis, MN, USA, Cat no: 5046) or vehicle solution consisting of DMSO during application of 6-OHDA ...
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bioRxiv - Cell Biology 2020Quote: ... using recombinant carrier free ACE2 (# 933-ZN-010) and Skp1/Skp2 (#E3-521-025) proteins bought from R&D Systems (Minneapolis, MN, USA) or Flag-ACE2 and Flag-Skp1/Skp2 purified from 293T cells ...
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bioRxiv - Neuroscience 2021Quote: ... Nrp1-Fc fusion protein containing the extracellular domain of rat Nrp1 (amino acid 22-854 minus 811-828; R&D Systems, 566-NNS) or the control human Fc dimer (R&D Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... 50μl of mitochondria (1μg/μl protein) were treated with Caspase-8-cleaved tBid from 5-500nM (882-B8-050, R&D Systems, Minneapolis, MN), Bim-BH3 from 10-10,000nM (Ac-DMRPEIWIAQELRRIGDEFNAYYARR-amide ...
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bioRxiv - Developmental Biology 2021Quote: ... 2014) has a 42% sequence identity and 58% sequence similarity with the recombinant zebrafish BMP4 protein (R&D Systems, cat. 1128-BM-010) used ...
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bioRxiv - Cancer Biology 2020Quote: ... Presence of 105 proteins in supernatants was evaluated using human cytokine and chemokine XL proteome array kit from R&D Systems (Minneapolis, MN).
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bioRxiv - Neuroscience 2022Quote: ... progranulin concentrations were determined in duplicate using 10–15 μl of lysates per well (typically 8–20 μg of total protein per well) using a sandwich ELISA assay (R&D Systems, DPGRN0). For experiments analyzing secreted progranulin ...
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bioRxiv - Cancer Biology 2022Quote: ... with good affinity and specificity were screened and selected from the homemade human single-chain antibody library through commercial VEGF and EGFR proteins (R&D Systems, Inc.). Then ...
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bioRxiv - Immunology 2021Quote: The expression of NKG2D ligands in tumor cell lines was assessed using Recombinant Human NKG2D Fc Chimera Protein (R&D systems, Abingdon, UK). 105 tumor cells were incubated either with 0.5 μg of NKG2D Fc recombinant protein or IgG1-Fc during 30 min ...
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bioRxiv - Microbiology 2021Quote: Sterile tissue culture plates were coated overnight at 4°C with 5 µg/mL recombinant CD62P protein (R&D Systems, #137-PS-050). The following day the wells were washed 3X with PBS before being blocked with 1% BSA in PBS at room temperature (RT ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies targeting the following proteins were used at the indicated dilutions and obtained from the denoted companies: Robo3 1:300 (R&D systems, #AF3155), GluN1 NMDAR 1:1000 (SySy #114011) ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: ICAM-1 and VCAM-1 protein levels were evaluated by SDS PAGE using goat anti-Icam-1 (R&D systems Cat# AF-796) and rabbit anti-Vcam-1 (abcam ...
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bioRxiv - Systems Biology 2023Quote: Where indicated the cells were stimulated with the final concentration of 10 ng/ml of recombinant human TNF-α protein (R&D Systems) or 100 ng/ml of recombinant human interleukin 1 beta (IL-1β ...
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bioRxiv - Developmental Biology 2023Quote: ... Subsequently cell clusters were differentiated into β-like cells by exposure to the appropriate media as previously published.29 All recombinant proteins were purchased from R&D System unless otherwise stated (see Supplementary Table 3).
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bioRxiv - Physiology 2023Quote: ... ciCMVEC were placed in serum-free media 1h before treatment with 10 ng/ml of Recombinant Human TNF-α Protein (210-TA-005; R&D systems) or vehicle (PBS ...
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bioRxiv - Cell Biology 2024Quote: Secreted proteins were measured in cell culture supernatants from treated iBMDMs using ELISAs for IL-1β and CCL5 (Quantikine®, R&D systems).
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bioRxiv - Cancer Biology 2023Quote: ... the quantification of epidermal growth factor (EGF) protein levels in the serum was performed with the Mouse EGF Quantikine ELISA Kit (MEG00, R&D Systems Inc.) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... the cells were cultured in N2B27 with 10 ng/ml mouse basic fibroblast growth factor (bFGF) recombinant proteins (R&D systems #313FB025/CF). On Day 2 ...
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bioRxiv - Bioengineering 2024Quote: ... IDUA activity was assessed using 4-methylumberlliferyl-alpha-L-iduronide (Glycosynth, Cheshire, England) and recombinant human alpha-L-iduronidase protein (R&D systems, Minneapolis) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... A subset of slices were infected with only AAV9.hsyn.GFP and at DIV6 were treated with human Sema4D-Fc ectodomain/human Fc fusion protein (Sema4D-Fc; R&D Systems, #7470-S4) or Fc control protein (R&D Systems ...
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bioRxiv - Genomics 2022Quote: Circulating AAV-expressed human ANGPTL3 protein levels were measured from plasma using commercially-available ELISA kits (plasma diluted 1:4 and used with R&D Systems #DANL30). The assay is specific for detection of human ANGPTL3 protein ...
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bioRxiv - Microbiology 2022Quote: ... The HA remaining after supernatant-driven degradation was detected with HA binding protein following dilutions and instructions in the Hyaluronan DuoSet ELISA kit by R&D Systems (DY3614). As was the case for fibronectin and laminin ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were left for 48h to allow expression of LepRb-HA protein before treatment with recombinant murine leptin (R&D System #498-OB) at 0-10 ng/L for 30 minutes before cell lysis and western blotting for phosphoSTAT3 (Y705) ...
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bioRxiv - Cancer Biology 2023Quote: ... POSTN protein expression in the medium was confirmed using an ELISA assay with an anti-POSTN polyclonal antibody (R&D Systems, Cat # AF3548). 3T3-CTL cells were used to generate “control” conditioned medium (CMCTL) ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was collected at indicated times and protein concentrations were determined by ELISA following the manufacturer’s instructions for IL-6 (R&D systems, DY406-05), TNFα (R&D systems ...
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bioRxiv - Neuroscience 2024Quote: Phosphorylation levels were measured in remaining hDRG protein samples using the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems, CAT# ARY003C). The commercial protocol was followed ...