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Citations for R&D Systems :
751 - 800 of 3404 citations for 4 Chloro 6 fluorobenzene 1 3 diamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... and incubated with rabbit active Caspase-3 (5 μg/ml, AF835-SP, R&D systems, Inc., Minneapolis, MN), rabbit cleaved Caspase-9 (1:50 ...
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bioRxiv - Immunology 2023Quote: ... 2 ng/mL of IL-2 and 3 ng/mL of TGFβ (R&D systems, 7666-MB-055)) conditions ...
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bioRxiv - Microbiology 2020Quote: Mouse CXCL1/KC and interleukin-6 concentrations in lung homogenates from infected mice were determined using DuoSet® ELISA kits (R&D Systems, UK) according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... offspring at postnatal day 3 to 6 received 7 intraperitoneal injections with either PBS (controls) or 75 ng of carrier free recombinant mouse interleukin-6 (rmIL6, R&D Systems, Minneapolis MN).
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bioRxiv - Cell Biology 2020Quote: ... IL-6 and TNFα cytokine levels in the media were then measured with DuoSet ELISA kits (R&D systems, catalog number DY406 and DY410) according to manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... IL-6 was detected in culture supernatants from MSC/T cell co-culture assays by magnetic Luminex assays (R&D Systems, Biotechne, Abingdon, UK) as previously described [31] ...
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bioRxiv - Physiology 2021Quote: ... primary osteoclast precursors were seeded after initial culture on non-treated plates into 6 well plates in the presence of 30ng/mL recombinant mouse M-CSF (R&D systems, Minneapolis, MN) for 3 days ...
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bioRxiv - Immunology 2022Quote: ... IL-6 cat # D6050, IL-1Ra cat # DRA00B, IFN-γ cat #DY285B, IL-10 cat #DY21YB, IL-17 cat# DY317, R&D Systems, USA), following the instructions of the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... and 50 μg/ml L-Ascorbic Acid)) supplemented with Growth Factor Cocktail (5 ng/ml BMP4, 5 ng/ml bFGF, 10 ng/ml IL-6 (R&D systems, 206-IL), 10 ng/ml Flt3L (PeproTech ...
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bioRxiv - Molecular Biology 2024Quote: ... for 30 min at room temperature and then probed with the following primary and secondary antibodies: anti-Mbd4 (Santa Cruz Biotech D-6 or R&D Systems AF5935), anti-Msh6 (AbCam EPR3945) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated over night at 4 °C with the anti-CD140a (R&D Systems AF1062) diluted 1:80 in blocking solution ...
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bioRxiv - Immunology 2021Quote: ... were coated with recombinant human CTLA-4-Fc (7268-CT, R&D Systems, Minneapolis, MN, USA) at 1 μg/mL overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 100 ng/ml Act A + 50 ng/ml bone morphogenetic protein-4 (BMP4, R&D Systems), (iv ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated with 4 µg of purified GST-tagged ISG15 (R&D systems, UL-600-500) or GSTP1 (#G5663 ...
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bioRxiv - Cancer Biology 2022Quote: ... and then incubated overnight at 4°C with goat polyclonal antibody to ST6GAL1 (R&D Systems) (see Table S3 for antibody information) ...
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bioRxiv - Molecular Biology 2022Quote: ... M2 macrophages were generated by stimulating Mϕ with 20 ng/ml IL-4 (R&D Systems) and 20 ng/ml IL-13 (R&D system ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were harvested after 4 h and assayed for IFN-γ via ELISA (R&D Systems).
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bioRxiv - Physiology 2023Quote: ... Sections were then stained overnight at 4°C using primary antibody for SOX9 (R&D Systems) or phosphor-EGFR (phosphoTyr-1173 ...
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bioRxiv - Physiology 2024Quote: ... then stimulated with recombinant human IL-4 at 50 ng/mL (R&D Systems, Minneapolis, MN) in BEBM medium for 6 h or BEBM medium as control condition ...
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bioRxiv - Immunology 2023Quote: ... and stimulated with 0.3 μg/ml HEL plus 20 ng/ml IL-4 (R&D Systems), 0.2 μg/ml CpG (InvivoGen ...
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bioRxiv - Genomics 2023Quote: ... membranes were probed overnight at 4°C with antibodies that recognize ERAP2 (AF3830, R&D Systems) or α-tubulin (T9026 ...
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bioRxiv - Immunology 2024Quote: ... in conjunction with neutralizing antibodies against mouse IFNγ and IL-4 (also from R&D Systems). Culturing was performed in IMDM medium (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... Mycoplasma testing was performed every 3 months with the MycoProbe mycoplasma detection kit from R&D systems (Minneapolis, MN). None of the cell lines used in this study are found on the misidentified cell lines list from the International Cell Line Authentication Committee.
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bioRxiv - Cancer Biology 2021Quote: ... Negative control cells were produced by treatment with 10 μM Z-DEVD-FMK caspase-3 inhibitor (R&D systems). 200μl of cell suspension from each cell line including the negative control group was transferred into a flow cytometry tube before being treated with 2.5μl of 0.2mM NucViewTM 488 caspase-3 substrate (fluorogenic DNA dye and DEVD moiety substrate for caspase-3 ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.03 to 3 μg/well of the soluble mouse or human HA-tagged recombinant fibulin 7 (R&D Systems) in 2% dry milk/TBS and 2 mM CaCl2 were added to the wells and incubated for 3 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were tested for Mycoplasma routinely every 3 months using MycoProbe Mycoplasma Detection Kit (R&D Systems).
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bioRxiv - Cancer Biology 2022Quote: ... Digested cells were washed three times with PBS and resuspended in 2/3 Cultrex Type 2 (R&D Systems) and 1/3 Full Ad-DF+++ medium and plated in drops which were allowed to solidify for 30 minutes at 37°C after which they were overlayed with Full Ad-DF+++ medium ...
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bioRxiv - Microbiology 2023Quote: ... coli were made from an overnight agar culture and incubated with 3 μM HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
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bioRxiv - Immunology 2024Quote: ... and Cl13-infected mice was measured by Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit (R&D Systems), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The mice remained on Western diet for two more weeks before euthanasia and evaluating serum concentrations of TNF-α and IL-6 via enzyme-linked immunosorbent assay (ELISA, R&D Systems, Minneapolis, MN).
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bioRxiv - Biochemistry 2022Quote: ... mice aged 6.5 weeks were starved for 6 hours and intraperitoneally injected with 220ng/g body weight of recombinant mouse FGF23 (2629-FG-025 R&D Systems, Minneapolis, MN, USA) in PBS-BSA 0.1% or PBS-BSA 0.1% vehicle with injection volume of 4μL/g body weight ...
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bioRxiv - Neuroscience 2022Quote: ... a complete media change was performed with CDMIII containing respective compounds at a final concentration of 8.8 ng/ml (422.6 nM) IL-6 (R&D Systems, Cat. no. 7270-IL-025) and 25 ng/ml (422.6 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded in 6 well plates and then mock-treated or treated with 5000 U/mL of human recombinant IFNβ (R&D systems, 8499-IF/CF) for 24 hours.
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bioRxiv - Cell Biology 2022Quote: ... The concentrations of IL-6 and TNF alpha in cell culture supernatants was measured by using the Mouse IL-6 and TNF alpha DuoSet ELISA (R&D systems, Minneapolis, Minnesota, USA). Statistical differences in LDH release and cytokine secretion between RAW 264.7 macrophages challenged with A ...
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bioRxiv - Immunology 2023Quote: ... the cells were plated at 0.2M cells/ml in a 6-well plate and stimulated with IFNβ (500 IU/ml, R&D systems cat. 8499-IF-010). At 24h ...
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bioRxiv - Cell Biology 2024Quote: ... The concentration of IL-6 (DY206) and VEGFA (DY293B-05) in the conditioned media was measured by using ELISA DuoSet Kit (R&D Systems, Minneapolis, MN, USA). The concentration was calculated by following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... PNMI biomarker assays were conducted using high-sensitivity (IL-6, IL-8) and regular sensitivity (sTNF-RII, IL-1RA) ELISAs (R&D Systems, Inc., Minneapolis, MN), and raw concentrations were reported in picograms per milliliter (pg/mL).
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bioRxiv - Cell Biology 2024Quote: ... Marrow was flushed from femurs and cultured for 6 days in bone marrow medium (BMM): RPMI medium supplemented with recombinant murine CSF1 (50 ng/ml; R&D Systems, #416-ML-500), 50 mM b-mercaptoethanol ...
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bioRxiv - Cancer Biology 2024Quote: ... Some CD8+ T cells from C57BL/6 mice were directly exposed to 50ng/mL recombinant murine VEGF from R&D Systems (493-MV-005/CF) in the absence of tumor cell co-culture as a control condition ...
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bioRxiv - Physiology 2024Quote: ... rmICAM-1 (ICAM-1 Fc chimera; 15µg mL−1; R&D Systems), and rmCXCL1 (15µg mL−1 ...
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bioRxiv - Cell Biology 2020Quote: ... After 4 days cells were re-stimulated and cytokine production was measured by ELISA (R&D Systems).
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bioRxiv - Neuroscience 2020Quote: ... 4) incubation with a donkey anti-rabbit IgG conjugated with NorthernLights™ NL557 (#NL004, R&D System) diluted 1:1000 for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and stained overnight at 4°C with goat anti-human SPP1 antibody (R&D Systems, AF1433-SP), followed by secondary staining with donkey anti-goat AF594 (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2022Quote: ... n = 4 each) were stained with mouse anti-SARS-CoV-2 N monoclonal antibody (R&D systems, Clone 1035111 ...
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bioRxiv - Pathology 2024Quote: ... Cells were then immunolabeled for 4hr at 4°C with sheep anti-CD31 (R&D Systems, AF806) + rabbit anti-cTNT (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant human IL-4 500 IU/mL and GM- CSF 800 IU/mL (both R&D systems) at a cell density of 1-2 x106 cells/mL for 5 days ...
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bioRxiv - Neuroscience 2024Quote: ... or 4 ng recombinant human ADAMTS4 (R&D Systems 4307-AD; 2 ng/side in 100 nl) were performed at ZT5 using bevelled stainless steel 33-gauge injectors ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μl of 10 mg ml-1 ubiquitin (1 mg ml-1 final) (R&D Systems, U-100H), 0.5 μl of 200 mM DTT ...
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bioRxiv - Bioengineering 2022Quote: ... angiopoietin-1 (ANG-1, R&D Systems) and hepatocyte growth factor (HGF ...
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bioRxiv - Cell Biology 2022Quote: ... NKX6-1 (1:1000; R&D Systems), NR4A2 (NURR1 ...