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Citations for R&D Systems :
7601 - 7650 of 10000+ citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: IGF-1 levels in the serum and tissues (liver, gastrocnemius, and brain cortex) were determined using Quantikine mouse IGF-1 immunoassay from R&D systems (MN, USA). Serum samples were diluted in calibrator diluent provided in the kit at 500-fold dilution and assayed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Non-specific blocking for 30 minutes in 8% horse serum in KPBS was completed before incubation in primary antibody (anti-HPLN1 1:150, R&D Systems 2608-HP) for 48 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... or the newly designed FRET Aβ34-site peptide (10 µM) were digested at 37° C by human BACE2 (R&D systems, 1 ng/µL) in presence or absence of the stated inhibitors for 2h ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) with 0.1% Tween 20) for 30 minutes and probed with primary antibodies: polyclonal goat anti-C3d (R&D systems, AF2655, 1:1000), polyclonal sheep anti-Sez6L2 (R&D Systems ...
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bioRxiv - Microbiology 2020Quote: ... fourth weeks of infection were collected to evaluate the concentration of MCP-1 and TNF-α by using ELISA kits according to the manufacturer’s protocols (R&D Systems, Rennes, France), the mean minimum detectable dose of human MCP-1 was 1.7 pg/mL and 4.00 pg/mL for human TNF-α ...
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bioRxiv - Genomics 2020Quote: Plasma was used to assess circulating monocyte chemoattractant protein (MCP)-1 and C-reactive protein (CRP) via ELISA following manufacturer’s instructions (R&D Systems, Minneapolis, MN, USA) (1).
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bioRxiv - Cancer Biology 2021Quote: ... the culture media were replaced with fresh media (DMEM + 10% FBS) supplemented with 0.03 ng ml−1 of recombinant mouse IFNγ (R&D Systems, #485-MI-100). After 24 h treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... Then membranes were blocked in 5% non-fat milk in TBS-T for 30 minutes at room temperature and incubated with primary antibodies against Robo1 (R&D Systems, 1:500), Robo2 (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... The cells were fixed and stained as described in (Rijal et al. 2019) using a 1:500 dilution of anti-CD66 (#MAB2244, R&D Systems, Minneapolis, MN), anti-CD62-L (#555542 ...
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bioRxiv - Cell Biology 2022Quote: ... and stained for 30 minutes on ice with a 1:50 dilution of AlexaFluor-647 conjugated anti-LTB4R antibody (R&D Systems; catalog #: FAB099R) in FACS buffer (0.5% bovine serum albumin and 0.05% sodium azide in PBS) ...
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bioRxiv - Systems Biology 2021Quote: ... The concentration of CCN4 in the cell culture media from those wells was assayed using the Human WISP-1/CCN4 DuoSet ELISA Kit (R&D Systems, Minneapolis, MN) to confirm CCN4 knockout ...
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bioRxiv - Bioengineering 2020Quote: ... medium was removed and the scaffolds designated for study in normal growth media were placed in wells with fresh normal growth medium and the mineralized collagen-AM scaffolds designated for study in inflammatory conditions were placed in wells with inflammatory medium (1 ng/ml of IL-1β recombinant porcine protein (R&D Systems, Minneapolis, MN) added to normal growth media) ...
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bioRxiv - Cell Biology 2021Quote: ... directly after UV irradiation or pre-treated 1 hour before irradiation with proteasome inhibitor MG132 (Enzo, 50 µM) or NEDD8 E1 Activating Enzyme Inhibitor (NAEi) MLN4924 (R&D systems, 10 µM) where indicated ...
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bioRxiv - Cell Biology 2020Quote: ... Retinal crossections (12 µm) were processed for immunostaining using the following antibodies: rat monoclonal to CD45 antibody (clone 30-F11, R&D Systems, 1:50). Sections were pre-incubated with 5% goat serum (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with anti-CD31 primary antibody (1:200, #550274, BD Biosciences, San Jose, CA, USA; #BBA7, R&D systems, Minneapolis, MN, USA) and Alexa Fluor 594-conjugated secondary antibody.
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bioRxiv - Microbiology 2021Quote: ... Domes were dislodged with a cell scraper in 1 mL of Cultrex Organoid Harvesting solution (Bio-techne, R&D Systems brand, 3700-100-01) and incubated for 1 hr at 4°C on a shaker at 250 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in PBS and incubated for 30 minutes at room temperature (RT) with the human α-PlexinD1 mouse monoclonal antibody (1:150 dilution) (R&D systems, MAB4160). Cells were washed twice with PBS and incubated for 30 minutes at RT with an α-Mouse IgG secondary goat antibody conjugated to the APC fluorochrome (1:200 dilution ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... the samples were then longitudinally oriented and cut to 15µm thickness before being stained with primary antibodies to mouse CD31 conjugated to Alexa Fluor 488 (R&D Systems, #FAB3628G, 1:100), CD31 (BD Pharmingen ...
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bioRxiv - Neuroscience 2023Quote: ... The cell lysates (20 μg) were electrophoresis using SDS gels and transferred to nitrocellulose membranes and the incubated with TrkB antibody (1:1000, R&D systems, goat, AF1494), CRHR1 antibody (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: Cortical neurons were treated with either 1.5 µM Aβ or rat recombinant TIMP-1 peptide (R&D systems, Cat# 580-RT-010, Minneapolis, USA) at 100 ng/ml32 or a combination of both for 16 h as shown in figures.
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bioRxiv - Bioengineering 2024Quote: A total of 1 μg Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) was dissolved in GelMA/fibrin hydrogel containing thrombin and transferred into a cylinder-shaped mold with a liquid capacity of 8.6 μl and 1 mm diameter with 1 mm thickness ...
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bioRxiv - Neuroscience 2022Quote: ... The cannula was connected to an osmotic minipump filled either with vehicle solution (PBS containing 0.1% BSA as a carrier) or 35 μg/ml of soluble TNF receptor-1 (sTNFR1, R&D Systems Inc. Minneapolis, MN).
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bioRxiv - Microbiology 2023Quote: ... Huiskonen (University of Oxford) and incubated for three days at 37 °C in the presence of 1 μg/ml of kifunensine (R&D Systems, MN, USA). Supernatant was collected and combined with half a volume of binding buffer (25 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... TF1 cells were cultured in RPMI-1640 with 10% FBS and 1 ng/mL recombinant human granulocyte–macrophage colony-stimulating factor (rhGM-CSF, No. 215-GM, R&D Systems, Minneapolis, MN). 293T cells were cultured in DMEM media containing 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: Human CCL2/MCP-1 and M-CSF/CSF1 were measured by ELISA using DuoSet kits (DY279, DY216, respectively) from R&D Systems (Minneapolis, USA) according to the manufacturer’s recommendations.
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bioRxiv - Developmental Biology 2024Quote: ... Agents were added to the medium at final concentrations of 1 μg/mL for BMP4 treatment (314-BP; R&D Systems, Minneapolis, MN, USA), 1% v/v of 4 mM HCl containing 0.1% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... the cell media were exchanged with 400 µL ice-cold DMEM media containing 10 % FBS and 1 µg/mL α-TrkB antibody (goat anti human/mouse TrkB, R&D Systems #AF1494). The cells were incubated on ice for 2 hours ...
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bioRxiv - Microbiology 2024Quote: Secreted IL-1β or IP-10 in culture supernatants was measured using a commercially available Human IL-1 beta/IL-1F2 DuoSet ELISA kit (R&D Systems, catalog #DY201) or Human IP-10 ELISA Set (BD Biosciences) ...
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bioRxiv - Genomics 2024Quote: FACS-sorted Vcam1-FAPs were grown to 60-80% confluence followed by adipogenic differentiation and were treated with 500 ng/mL recombinant mouse sFRP-1 protein (R&D systems, 9019-SF) with or without sFRP-1 inhibitor ...
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bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation with the primary antibody for platelet-derived growth factor receptor alpha (PDGFRa) (ref. AF1062-SP, R&D systems, 1/200), OLIG2 (ref ...
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bioRxiv - Physiology 2024Quote: ... protein levels in cell culture supernatants of hCMEC/D3 after stimulation with tumor necrosis factor-α (TNF-α) was conducted utilizing a commercially available human VCAM-1/CD106 DuoSet ELISA kit (R&D Systems). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: H3K27M-altered human DMG cells (Supplementary Table 1) were grown under adherent stem cell conditions using flasks coated with laminin (10ug/ml) (R&D systems, Bio-techne) in tumour stem media complete (TSM-C) ...
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bioRxiv - Developmental Biology 2022Quote: ... Following primary antibodies (αTFAP2A, Abcam ab108311; αTFAP2B, Abcam ab221094; αLEF1, Cell Signaling, 2230; αSOX2, R&D Systems, AF2018; αPITX1, Abcam ab70273; and αPITX2, R&D Systems, AF7388) were diluted in TBS (0.5% Tween in phosphate-buffered saline ...
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bioRxiv - Developmental Biology 2021Quote: ... and 20 ng/ml FGF2 (R&D systems, 233-FB-025) referred to from now on as NMP differentiation medium ...
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bioRxiv - Cell Biology 2021Quote: ... or HB-EGF (100 ng/ml; R&D Systems, Minneapolis, MN) as indicated in the Figure legends ...
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bioRxiv - Developmental Biology 2021Quote: ... BMP4 recombinant protein (R&D Systems, Catalog No. 314-BP-050) was diluted in 15% pluronic gel (prepared in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... and 20 ng/ml FGF2 (R&D Systems, 233-FB- 01M) in CDM2 basal media ...
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bioRxiv - Cell Biology 2020Quote: ... Treatment conditions included IL-13 (20 ng/ml, R&D Systems), HSP90 inhibitor geldanamycin (25 μM ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-goat IgG HRP (HAF109, R&D Systems MI, USA) were used as secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... LPS (10ng/ml) or IL-1b (5ng/ml) (R&D systems) or with ‘anti-inflammatory’ compound IL-4 (10ng/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... 100 ng/ml recombinant FLT1-FC (R&D Systems, 7756-FL), 1 µg/ml anti-FLT1 monoclonal antibody (Angio-Proteomie ...
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bioRxiv - Immunology 2021Quote: ... and a mouse anti-CD9 monoclonal antibody (#MAB1880, R&D Systems). These antibodies were biotinylated using an EZ-Link micro Sulfo-NHS-biotinylation kit (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... and human IL-1β/IL-1F2 DuoSet ELISA (R&D systems). Twenty-two cytokines were detected using Bio-plex human chemokine ...
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bioRxiv - Immunology 2021Quote: ... IFNλ1 (100 ng/mL; 1598-IL-025, R&D Systems, USA); and Ruxolitinib (10 µM ...
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bioRxiv - Developmental Biology 2021Quote: ... or anti-FST antibody (20 μ/ml, AF669, R&D Systems) for 42 hr ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with lysis buffer 17 (R&D Systems, 895943) and protein quantified using bicinchoninic acid protein assay (BCA ...
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bioRxiv - Developmental Biology 2021Quote: ... 50 ng/ml Bmp8a (R&D Systems, cat. 1073-BP-010), 2 ng/ml SCF (R&D Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 ng/ml EGF (R&D Systems, cat. 2028-EG-010) and 1000 U/ml ESGRO (Millipore ...
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bioRxiv - Immunology 2020Quote: ... IL-15Rα alone (IL-15Rα Fc chimera protein, R&D Systems) or IL-15Rα pre-complexed with IL-15 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... Biotin goat anti-mouse polyclonal Ephrin-B1 (BAF473) (R&D Systems); FITC anti-BrDU (11-5071-42 ...