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Citations for R&D Systems :
701 - 750 of 978 citations for N6 2 aminoethyl 9H purine 2 6 diamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... PBS was removed and replaced with 200 µL of blocking solution (dPBS with 2% (v/v) BSA) containing α-TIM1(1:100, AF1750, R&D Systems) or α - AXL antibody (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... culture medium was supplemented with 0.1 to 0.5 % BME (Cultrex Reduced Growth Factor Basement Membrane Extract, type 2, Pathclear, R&D Systems, 3533-005-02). We observed batch-to-batch variations of this product ...
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bioRxiv - Immunology 2022Quote: ... were incubated for 30 min at 20°C in 50 μl RPMI-HEPES in the absence of BCS with 2 μg/ml anti-ACE2 antibody (R&D Systems, #AF933) (Hoffmann et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were blocked in 2% goat serum for 45 mins at room temperature and incubated with anti-Snail (R&D Systems, Minneapolis, MN), anti-Zeb1 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... and then brought to 1-2 million PBMCs/mL in R10 buffer plus 500pg/mL rhIL-7 (R&D Systems, Minneapolis, MN, USA) and rested again for four days ...
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bioRxiv - Physiology 2020Quote: ... 0.1 mL/10 g body weight) on day 1 and leptin (2 mg/kg; 0.1 mL/10 g body weight; CAT# 498-OB, R&D Systems, Minneapolis, MN) on day 2 at 6am ...
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bioRxiv - Immunology 2021Quote: ... Cyclooxygenase-2-derived prostaglandin E2 (PGE2) and LTB4 release were measured with enzyme-linked immunosorbent assay kits (ELISA; R&D systems, Minneapolis, MN) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 2) anti-CD31 antibodies (#550274, BD Biosciences, San Jose, CA, USA; #ab28364, Abcam, Cambridge, MA, USA; #BBA7, R&D systems, Minneapolis, MN, USA); 3 ...
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bioRxiv - Cell Biology 2021Quote: ... Media was replaced after 48 hours (Day 2) with RPMI-1640 supplemented with B27 without insulin and 5 µM IWP2 (R&D Systems #3533). Media was again replaced after an additional 48 hours (Day 4 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies (DCX, Goat anti-doublecortin, 1:500, Santa Cruz; SOX2, Goat anti-sex determining region Y-box 2, 1:200, R&D Systems; CLR ...
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bioRxiv - Neuroscience 2023Quote: ... 3mg of nuclear lysates were used for IP with 2 μg IgG or 5 μg primary antibodies (normal mouse IgG, Santa Cruz Biotechnology, sc-2025; normal rabbit IgG, R&D Systems, AB-105-C ...
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bioRxiv - Microbiology 2023Quote: ... Murine IP-10 production was measured from 100 μL of the supernatant from the BMDMs using Mouse CXCL10/IP-10/CRG-2 Duo Set ELISA (R&D systems, #Dy466) according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2023Quote: ... and rTv20S-inhibitor complex were diluted with 50 mM HEPES pH 7.5 then mixed with 2 μM MV151 (R&D Systems #I-190). After MV151 addition ...
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bioRxiv - Cell Biology 2022Quote: ... viability was measured with the BioTek Synergy HT plate reader using Gen5 software after a 2 hour incubation in Alamar blue (R&D Systems, AR002). Wells without cells and incubated in Alamar blue were used as a background reading.
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bioRxiv - Bioengineering 2023Quote: ... Plates were incubated with PBSTBA diluted 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) for 2 h at RT ...
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bioRxiv - Cell Biology 2024Quote: ... slides for ACE-2 single stain and slides for ACE-2 stain were incubated with the Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Secondary Antibody (1:100, NL004, R&D system) and the mixture of Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Secondary Antibody (1:100 ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed twice with cold FACS buffer and consequently incubated with anti-mouse F(ab)2 IgG (H+L) PE-conjugated antibody (R&D systems, F0102B) for 1h in the dark at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... small molecules (CHIR99021[2 mM, Axon, Groningen, Netherlands]; SB-431542[10 mM, Axon, Groningen, Netherlands]; noggin[0.5 mg/ml, R&D Systems R&D Systems, Minneapolis ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were cultured in media containing 2% FBS and treated with recombinant human Wnt-3a protein (R&D systems, Cat# 5036-WN-010) at a final concentration of 200ng/mL for 2h.
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bioRxiv - Cancer Biology 2024Quote: ... and 10 IU/ml of penicillin/streptomycin) containing 1,000 U/ml of murine recombinant IL-2 (R&D Systems, Cat# 402-ML-020).
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bioRxiv - Microbiology 2024Quote: ... Supernatants were collected at 6 hr and TNF-𝛼 and IL-6 production were measured by enzyme-linked immunosorbent assay (R&D Systems, DuoSet ELISA).
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bioRxiv - Cancer Biology 2024Quote: ... Secreted Prostaglandin E2 (PGE2, #514010) and Interleukin-6 (IL-6, #583371, #501030) levels were measured using commercial ELISA kits (Cayman Chemical, MI USA, R&D Systems, MN, USA) and normalized to the cell number ...
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bioRxiv - Physiology 2020Quote: Serum samples were assayed for IL-6 concentration using commercially available human IL-6 enzyme linked immunosorbent assay kits (Quantikine®, R&D systems, Minneapolis, MN, USA) according to the manufacturer's instructions ...
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bioRxiv - Immunology 2021Quote: ... 2×105 isolated TILs have been cultured in vitro in 96well plate in presence of 20ng/ml mouse IL-2 (R&D System 402-ML), 20ng/ml mouse IL-7 (R&D System 407-ML ...
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bioRxiv - Cell Biology 2021Quote: ... Thy1+ Rep-MEFs were cultured in ‘iMPC medium’ containing 2 μg/mL of dox and administrated three small molecules: 5 μM of Forskolin (F) (R&D Systems, 1099/50), 5 μM of RepSox (R ...
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bioRxiv - Neuroscience 2020Quote: ... mouse PFC fractions were dissociated with a tissue homogenizer in 100 μl of 0.5 × cell lysis buffer 2 diluted in PBS (R&D Systems., Minnesota, United State). Tissues were lysed at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: Virus-inactivated serum samples or lung supernatants from SARS-CoV-2 VoC infected mice were added to a custom 8-plex Mouse Magnetic Luminex Assay (R&D Systems LXSAMSM-08) including IL-6 ...
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bioRxiv - Immunology 2022Quote: Cells diluted to 1 × 106 per microtiter plate well were incubated for 45 min at room temperature with a 1 in 100 dilution of a mouse IgG2a antibody to human/hamster ACE-2 (clone 171606 R&D Systems, Minneapolis, MN). After washing ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: ... fresh DMEM medium with reduced FBS (2%) was changed and the supernatant was harvested 24 hr later for IGF-1 and SDF-1α ELISA assays (R&D Systems, Minneapolis, MN).
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bioRxiv - Bioengineering 2022Quote: ... and from Days 2 to 5 CHIR99021 was withdrawn and replaced with 100 ng/mL Activin A (R&D Systems, 338-AC-050/CF). Media change was performed every day by discarding spent media ...
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bioRxiv - Immunology 2022Quote: ... Five million PBMC were suspended in 40 ml R10 supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050) together with 10 million K562-mbIL21-41BBL feeder cells irradiated with 100 Gy ...
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bioRxiv - Developmental Biology 2022Quote: ... re-plated and incubated in 10-cm non-adherent Petri dishes in cSFDM containing 2 ng/ml rhActivin A (R&D Systems, #338-AC), 3 ng/ml rhBMP4 (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems, 202-IL-050/CF). As a positive control ...
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bioRxiv - Cancer Biology 2023Quote: ... supernatants from the A20 and DO-11.10 cells were collected for IL-2 detection by enzyme-linked immunosorbent assays (ELISA) using commercially available sandwich-based kit (R&D systems, Cat# M2000), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 50 mL of sample diluted 1:2-1:10 depending on the experiment or human TREM2-Fc standard (R&D Systems #1828-T2-050) were added to the plate and incubated at room temperature for one hour with shaking ...
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bioRxiv - Microbiology 2024Quote: ... mouse anti-SARS-CoV-2 spike S2 subunit mAb (IgG2a, Cat#: MAB10557) and mouse anti-SARS-CoV-2 nucleocapsid protein mAb (IgG2b, Cat#: MAB10474) were from R&D Systems (Minneapolis, MN). Mouse anti-SARS-CoV-2 membrane protein mAb E5A8A (IgG1 ...
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bioRxiv - Microbiology 2021Quote: ... 10 ng/mL IL-6 (206-IL-050, R&D Systems), 10 ng/mL VEGF and 30 ng/mL DKK-1(5439-DK-500 ...
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bioRxiv - Microbiology 2021Quote: ... IL-6 (10 ng/mL, all from R&D Systems, MN) and PGE2 (1 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... or CXCL9 (6 μg/ml, #AF-492-NA, R&D Systems) were added to the co-culture system 30 minutes before MPLA with IFNγ.
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bioRxiv - Cancer Biology 2021Quote: ... and 6 µg/mL anti-ULBP3 (clone 166510, R&D Systems) for 1h at 37°C prior to plating with γδ T cells ...
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bioRxiv - Immunology 2021Quote: ... and IL-6 secretion was assessed by ELISA (R&D Systems). Each semi-prep sample (15 µg/ml ...
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bioRxiv - Immunology 2021Quote: ... IL-6 (30 ng/ml; carrier-free; Tocris R&D Systems), IL-1β (10 ng/ml ...
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bioRxiv - Physiology 2022Quote: ... Cells were thereafter lysed with Lysis Buffer #6 (R&D Systems) and assayed for the expression levels of phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187 ...
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bioRxiv - Bioengineering 2024Quote: ... Human IL-6 Quantikine ELISA Kit (R&D systems, MN, USA), Mouse PBMC Isolation Kit (Solarbio ...
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bioRxiv - Molecular Biology 2023Quote: ... and Mouse IL-6 Quantikine ELISA Kit (M6000B, R&D Systems), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... IL-6 at 200 pg/ml (R&D system, 406-ML) was added to the basolateral compartment from day 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 ng/ml rhIL-6 (#206-IL, R&D Systems).30
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bioRxiv - Molecular Biology 2020Quote: ... the quantity of IL-2 released by Jurkat-CD16 cells was measured in the cell supernatant by colorimetry using a human IL-2 DuoSet ELISA DY202-05 Kit (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Bioengineering 2021Quote: ... After 48 hours the cells were fed with stage 2 differentiation medium consisting of STEMDiff APEL supplemented with 50 ng/mL of VEGF 165 (R&D Systems, 293-VE-010), 25 ng/mL of BMP4 (R&D Systems ...