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Citations for R&D Systems :
651 - 700 of 4230 citations for Heat Shock Factor Protein 1 HSF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with Alexa Fluor 488-conjugated anti-Lyve-1 antibody (1:500; FAB2125G, R&D Systems, Minneapolis, Minnesota, USA), rabbit anti-Prox-1 antibody (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... and stained with the different cell surface antibodies (1:10 anti-CD112-PE [clone R2.525, BD Biosciences], 1:10 anti-CD155-PE [clone 300907, R&D Systems] ...
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in appropriate cell culture medium as directed by supplier with 10% heat-inactivated fetal bovine serum (FBS) (R&D Systems; Minneapolis, MN) and 1% Penicillin-Streptomycin (Thermo Fisher ...
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bioRxiv - Bioengineering 2023Quote: ... either 5μg or 2.5μg of the S protein or NC protein (R&D Systems, cat# 10474-CV-050 and cat# 10549-CV-100) ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured for 96 hours with MOG35-55 peptide (50 μg/mL) and Th17-polarizing factors as follows: recombinant murine (rm)IL-23 (8 ng/ml; R&D Systems), rmIL-1α (10 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... and seeded in RPMI 1640 supplemented with 10% serum and 10 ng/ml recombinant macrophage colony-stimulating factor (M-CSF; R&D Systems) for 3 days ...
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bioRxiv - Immunology 2019Quote: ... from wild type and Irak1−/− mice were prepared by differentiation for 6 days in the same culture medium containing macrophage colony-stimulating factor (M-CSF; 60 ng/mL, R&D Systems). LPS was from Alexis Biochemicals (Salmonella minnesota R595 TLR grade ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were added to the culture plates at the density of 2.0 x 106 cells / dish in a final volume of 10 mL of complete RPMI-1640 supplemented with 20 ng/mL GM-CSF (granulocyte-macrophage colony stimulating factor; R&D Systems). After 3 days ...
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bioRxiv - Microbiology 2021Quote: ... gradient and treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) respectively for 7 days ...
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bioRxiv - Cell Biology 2020Quote: ... serum starved at 37°C in MV2 medium (containing no growth factors) 3 hrs before stimulation with VEGFA164 (50 ng/ml; R&D Systems), followed by fixation in 3% PFA for 3 min ...
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bioRxiv - Microbiology 2022Quote: ... cell media was replaced with media containing the compound and 20 ng/mL tumor necrosis factor alpha TNF-α (R&D Systems).
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bioRxiv - Neuroscience 2019Quote: ... for 24h or with 10 ng/mL tumor necrosis factor alpha and 10ng/ml IL1-ß(R&D Systems; 401-ML-005) for 24h ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Intestinal organoids were grown in base culture media (Advanced DMEM/F12 media, HEPES, GlutaMax, penicillin, and streptomycin) supplemented with growth factors (EGF, Noggin, R-spondin, R&D Systems), B27 (Life Technologies) ...
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bioRxiv - Immunology 2019Quote: ... normal T cell expressed and secreted (RANTES) and tumor necrosis factor alpha (TNF-α) by ELISA (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Intestinal organoids were grown in base culture media (Advanced DMEM/F12 media, HEPES, GlutaMax, penicillin, and streptomycin) supplemented with growth factors (EGF, Noggin, R-spondin, R&D Systems), B27 (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... bone morrow cells were cultured in Petri dishes in 10 ml DMEM cell culture medium containing 20 ng/ml of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems). On day 3 of culture ...
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bioRxiv - Microbiology 2021Quote: ... and then treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) for 7 days.
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bioRxiv - Immunology 2021Quote: ... IL-6 and tumour necrosis factor (TNF) in the supernatant were analysed by enzyme-linked immunosorbent assay (ELISA; DuoSet, R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... either vehicle (PBS) or candidate factors were added at the following concentrations: BDNF (5ng/mL, R&D Systems, Cat# 248-BDB-005), GDNF (5ng/mL ...
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bioRxiv - Microbiology 2022Quote: ... Tissues were minced and digested with collagenase to single cells before embedded in reduced growth factor extracellular matrix (Cultrex BME2, R&D Systems). Selective isolation of ductal stem cells was achieved using Isolation Medium (IM ...
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bioRxiv - Immunology 2022Quote: ... The differentiation to macrophages was initiated at day 0 by the addition of 20 ng/mL macrophage colony-stimulating factor (M-CSF, R&D Systems). Cells were cultured afterwards at 37°C and 5% CO2 for 7 days including the replacement of supplemented DMEM and replenishment of M-CSF at days 1 and 4 ...
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bioRxiv - Cell Biology 2024Quote: ... into a 96-well plate pre-coated with non-diluted Cultrex reduced growth factor base membrane extract (RGF-BME) (R&D Systems). The images of the network structure were captured by IncuCyte S3 real-time imager every 2 h at 4X magnification ...
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bioRxiv - Microbiology 2024Quote: ... and were differentiated into monocyte-derived macrophages (MDMs) by stimulation with 20 ng/mL macrophage colony-stimulating factor (M-CSF) (R&D Systems). MDMs were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Cell Biology 2024Quote: ... crypts per isolated from murine intestine and cultured in domes of Cultrex Pathclear Reduced Growth Factor Basement Membrane Extract (3533-005, R&D Systems), covered by ENR media comprised of Advanced DMEM/F12 (12634028 ...
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bioRxiv - Cell Biology 2024Quote: ... a suspension of 25,000 freshly dissociated cells or culture-expanded/FACS-sorted cells ranging from 625 to 5000 cells were prepared in growth factor reduced Matrigel (R&D Systems) and plated in 96-well plates as domes ...
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bioRxiv - Genetics 2023Quote: ... hiPSCs were plated onto Cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 25 mL flask (cat#353018 Falcon ...
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bioRxiv - Developmental Biology 2023Quote: ... washed 2x with PBS and seeded at a density 50 000 cells/50 μl reduced growth factor BME Type II (CultrexTM, R&D systems) in a 24-well cell culture plate (Corning ...
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bioRxiv - Microbiology 2022Quote: ... were obtained from healthy human blood donors after preparing blood monocyte monolayers and culturing for 2 days in medium containing macrophage colony-stimulating factor (R&D Systems) as described previously (56 ...
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bioRxiv - Systems Biology 2023Quote: ... on plates coated with 10 µg/cm2 Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane (R&D Systems #3434-010-02). Cells were cluster-passaged every 3-4 days with 0.5 mM EDTA and seeded into medium supplemented with 10 µM Y-27632 dihydrochloride (Bio-Techne #1254/10 ...
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bioRxiv - Bioengineering 2024Quote: MDA-MB-231 spheroids were formed starting from a culture of 10,000 cells per spheroid mixed with their medium and a 2% v/v Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BioTechne, R&D systems) solution in 96-well round-bottom plates (Corning Costar Ultra-Low Attachment Multiple Well Plate) ...
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bioRxiv - Immunology 2024Quote: ... the medium was enriched with recombinant murine interleukin-3 (IL-3; 5 ng/mL) and stem cell factor (SCF; 5 ng/mL) from R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2020Quote: ... anti-CD28 (1 µg/ml, clone CD28.2, eBioscience) and immobilized recombinant ICAM1-Fc fusion protein (0.5µg/ml, R&D Systems) in complete RPMI/HS medium ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibody against CA9 (AF2188; 1 μg/mL) was purchased from R&D Systems (Minneapolis, MN). Antibody against tubulin (T5168 ...
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bioRxiv - Biochemistry 2020Quote: ... Western blotting was performed with antibodies against human ANG (goat, R&D systems; 1:300), β-actin (rabbit ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plates were pre-coated with anti TRKB antibody (1:1000, #AF1494, R&D Systems) in carbonate buffer (pH 9.8) ...
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bioRxiv - Physiology 2021Quote: ... incubation buffer and TB4 antibody prediluted at 1:4000 (AF6796; R&D Systems, Minneapolis, MN,) were added to sterile borosilicate tubes and incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies anti-ACE2 (1:100, Mouse monoclonal, MAB933, R&D Systems, Minneapolis, MN USA) and anti-TMPRSS2 (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... We used the primary antibodies: Goat anti–mouse CD31 (AF3628; R&D Systems, 1:1000), Chicken anti–GFP (GFP10-20 ...
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bioRxiv - Bioengineering 2021Quote: ... Mouse anti-SARS-CoV-2 N monoclonal antibody (1:5000, R&D Systems, Cat#MAB10474) was used for the detection of SARS-CoV-2 ...
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bioRxiv - Bioengineering 2022Quote: ... goat polyclonal antibody to E-cadherin (R&D systems, catalogue no. AF748-SP, 1:250), mouse monoclonal Ab to CYP3A4 (3H8 ...
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bioRxiv - Pathology 2020Quote: ... probed with mouse monoclonal anti-cadherin-6 antibody (1:500, R&D Systems, catalog # MAB2715), then IRDYE 800CW donkey anti-mouse IgG (LI-COR Biosciences ...
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bioRxiv - Neuroscience 2019Quote: ... followed by primary antibody (anti-rabbit EphA5 1:250, R&D Systems Inc., Minneapolis, MN) diluted in blocking buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... Primary antibodies used were: goat anti-carboxypeptidase A1 (Cpa1) (1:1000 dilution, R&D systems); goat anti-ghrelin (Ghrl ...
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bioRxiv - Immunology 2019Quote: ... The sheep anti-CD38 polyclonal antibody (R&D Systems, Cat# AF4947, diluted at 1:100) and rabbit anti-F4/80 monoclonal antibody (Cell Signaling ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used: goat anti-Pax2 (1:500) (AF3364, R&D Systems), guinea pig anti-Lmx1b (1:10,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-human Pref-1/DLK1/FA1 APC-conjugated antibody (R&D Systems, FAB1144A; RRID:AB_2890004), and mouse anti-human CD10 (Biocare Medical ...
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bioRxiv - Neuroscience 2022Quote: ... ONs were incubated in PTwH + primary antibodies (anti-podocalyxin (1:500, R&D Systems – MAB1556) and anti-eGFP (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies were used: goat anti-Phox2b (R&D Systems, AF4940, 1:200), rabbit anti-RFP (Rockland ...
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bioRxiv - Neuroscience 2022Quote: Stimulation of organotypic slices with TNFα neutralizing antibody (1 μg/ml, R&D systems, MAB4101) was performed at DIV18-21 in pre-warmed aCSF (aCSF ...
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bioRxiv - Developmental Biology 2024Quote: The following antibodies were used: goat anti-PDGFR alpha 1: 200 (R&D Systems, AF1062), mouseanti-EZRIN 1:1000 (Sigma-Aldrich ...