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Citations for R&D Systems :
651 - 700 of 3499 citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and from Days 2 to 5 CHIR99021 was withdrawn and replaced with 100 ng/mL Activin A (R&D Systems, 338-AC-050/CF). Media change was performed every day by discarding spent media ...
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bioRxiv - Immunology 2022Quote: ... Five million PBMC were suspended in 40 ml R10 supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050) together with 10 million K562-mbIL21-41BBL feeder cells irradiated with 100 Gy ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
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bioRxiv - Developmental Biology 2022Quote: ... re-plated and incubated in 10-cm non-adherent Petri dishes in cSFDM containing 2 ng/ml rhActivin A (R&D Systems, #338-AC), 3 ng/ml rhBMP4 (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems, 202-IL-050/CF). As a positive control ...
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bioRxiv - Cancer Biology 2023Quote: ... supernatants from the A20 and DO-11.10 cells were collected for IL-2 detection by enzyme-linked immunosorbent assays (ELISA) using commercially available sandwich-based kit (R&D systems, Cat# M2000), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... mouse anti-SARS-CoV-2 spike S2 subunit mAb (IgG2a, Cat#: MAB10557) and mouse anti-SARS-CoV-2 nucleocapsid protein mAb (IgG2b, Cat#: MAB10474) were from R&D Systems (Minneapolis, MN). Mouse anti-SARS-CoV-2 membrane protein mAb E5A8A (IgG1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... in a 1:1 ratio with 100 U mL-1 of IL-7 and 10U mL-1 IL-15 (R&D Systems) in tissue culture flasks ...
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bioRxiv - Immunology 2024Quote: ... and 10ng/mL of recombinant human IL-7 (rh IL-7; R&D Systems). PBMCs treated with 0.1ug/mL of SEB were supplemented with 10ng/mL of rh IL-7 alone ...
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bioRxiv - Molecular Biology 2022Quote: ... dTAG-7 (R&D Systems), at the final concentration of 500 nM ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 5 ng mL-1 of IL-7 and IL-15 (R&D Systems). Regulatory T cells were activated at 1 x 106 cells mL-1 for 2 days in complete XVivo15 supplemented with magnetic Treg Xpander CTS Dynabeads (ThermoFisher ...
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bioRxiv - Bioengineering 2024Quote: ... and rhIL-7 (5 ng ml−1, Cat: 207-IL-010, R&D Systems). 1 x 104 post-EMO cells were combined with 2.5 x 105 MS5-hDLL4 stromal cells per ATO in a 1.7 ml Eppendorf tube (up to 144 ATOs per tube ...
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bioRxiv - Molecular Biology 2020Quote: ... the quantity of IL-2 released by Jurkat-CD16 cells was measured in the cell supernatant by colorimetry using a human IL-2 DuoSet ELISA DY202-05 Kit (R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Bioengineering 2021Quote: ... After 48 hours the cells were fed with stage 2 differentiation medium consisting of STEMDiff APEL supplemented with 50 ng/mL of VEGF 165 (R&D Systems, 293-VE-010), 25 ng/mL of BMP4 (R&D Systems ...
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) and incubated at 37°C/5% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) in AIM V (Thermo ...
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bioRxiv - Bioengineering 2022Quote: ... PBMCs were stimulated for 2 days with CD3/CD28 T cell activation beads (Thermo # 11131D) in the presence of 40IU/mL IL2 (R&D Systems # 202-IL-010) in AIM V (Thermo ...
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bioRxiv - Cancer Biology 2020Quote: ... Endothelial cells were seeded at a density of 2×105 per well on the insert coated with Cultrex rat collagen I (R&D Systems, Minneapolis, Minnesota, USA), and the insert was placed in the above-mentioned six-well plates ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and incubated with recombinant SARS-CoV-2 Spike protein receptor binding domain (RBD) fused to the human IgG1 Fc domain (R&D Systems 10499-CV-100) or control recombinant human IgG1 Fc protein (R&D Systems 10499-CV-100 ...
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bioRxiv - Immunology 2020Quote: ... For iTreg cell differentiation we additionally added the following cytokines and blocking antibodies: rmIL-2 and rmTGF-β (both: R&D Systems, 5 ng/ml), anti-IL-4 (clone ...
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bioRxiv - Immunology 2022Quote: ... Fecal LCN2 levels in the supernatant fraction were estimated using a Duoset murine LCN-2 ELISA kit (R&D Systems DY1857-05, Minneapolis, MN, USA) based on provided directions.
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bioRxiv - Physiology 2022Quote: ... and phospho-JNK (T183/Y185 for JNK1/2 and T221/Y223 for JNK3) with commercial kits (DuoSet IC ELISA, R&D Systems, # DYC1018B and # DYC1387B) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... hMEC 3D acini were resuspended in 10 mg/ml Cultrex Reduced Growth Factor Basement Membrane Extract (BME) Type 2 (R&D Systems, #3532-005-02) and 25 μl BME domes were plated in each well of µ-Slides 8 (Ibidi) ...
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bioRxiv - Microbiology 2023Quote: ... organoids were mechanically disrupted into single cells and plated in droplets of Cultrex growth factor reduced BME type 2 (Biotechne | R&D systems 3533-010-02). For the 2D culture of airway organoids ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed 2x with cold PBS + 2% FBS and stained with anti-CD127-Alexa Fluor® 488 (anti-IL-7Rα) (antibody clone 40131; R&D Systems Cat# FAB306G) for 30 min on ice ...
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bioRxiv - Bioengineering 2024Quote: ... were transferred into Neural Induction Media (NIM) composed of DMEM/F-12 supplemented with 1x N-2 MAX Media Supplement (R&D Systems, Cat no. AR009), 1x GlutaMAX ...
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bioRxiv - Developmental Biology 2024Quote: ... washed gently three times with warm DMEM-F12 and refed with mTeSR without ROCKi for 2 hours followed with mTeSR containing 50ng/ml BMP4 (R&D systems, #314-BP-010) for 42-48 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... or 5 days (SCLC cell lines) to allow for at least 2 cell doublings before processing in an MTT proliferation assay (R&D systems, #4890-050-K) performed according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... cell staining was done using the following conjugated antibodies and their IgG controls: Allophycocyanin (APC)-conjugated mouse IgG2a anti-human Neuropilin-2 (R&D systems, catalog no. FAB22151A) with allophycocyanin (APC)-conjugated mouse IgG2a control (R&D systems ...
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bioRxiv - Developmental Biology 2024Quote: ... The hPSCs were plated at ∼4x104 cells/cm2 and allowed to grow for 2 days before changing with “Day 0 medium” consisting of the “base medium” plus 20 ng/mL BMP4 (R&D Systems 314-BP-050), 30 ng/mL Activin A (R&D Systems 338-AC-050) ...
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bioRxiv - Bioengineering 2024Quote: HLOs in BME-2 were either collected manually by pipetting in cold PBS-/- or isolated from BME-2 using Cultrex organoid harvesting solution (R&D Systems; 3700-100-01) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 µM ZM306416 (R&D Systems, 2499/1) and 10 µM of SU5402 (R&D Systems ...
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bioRxiv - Physiology 2022Quote: ... rhuIL-3 (1 ng/ml, R&D Systems). Erythropoiesis was induced by addition of 3 U/ml recombinant human erythropoietin (rEPO ...
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bioRxiv - Cancer Biology 2022Quote: ... Caspase 3 (R&D Systems AF835, 1:800). The sections were then incubated with appropriate secondary antibodies for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: Caspase 3/7 activity was measured using a chromogenic DEVD cleavage assay according to manufacturer’s instructions (R&D Systems, Minneapolis, MN, #K106-100).
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bioRxiv - Molecular Biology 2020Quote: Randomly selected sections coming from ovarian tissue of least 3 different individuals were used for immunohistochemistry for Anti-active Caspase-3 (AC-3, 1:1000, AF-835, R&D Systems), γH2AX (1:250 IHC-00059 ...
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bioRxiv - Cell Biology 2020Quote: ... SCs were sorted based on positive alpha 7-integrin (1:100, R&D Systems FAB3518P) and absence of Sca1 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... NK cells were transferred to a round-bottom 96-well plate and resuspended in complete RPMI supplemented with 25 ng/mL rhIL-2 (R&D Systems, Cat. 202-IL-010). NK cells were then placed into a 37°C incubator ...
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bioRxiv - Pathology 2020Quote: ... cells of 2 MOI were treated with serum-free media for 24 hours and then media containing TGF-β (20 ng/ml, R&D Systems, Minneapolis, MN, USA) for 72 hours ...
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bioRxiv - Immunology 2024Quote: ... 2fTGH and HEK293T cells and ELISA was performed according to the manufacturer’s protocols for human IFN-β (R&D systems DY814-05PBL Assay Science 41410-2), human IFN-γ (Biolegend 43104 ...
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bioRxiv - Physiology 2024Quote: Wild type C57BL/6 10-week-old female mice were treated daily with recombinant tumor necrosis factor-like weak inducer of apoptosis (TWEAK 1237-TW; R&D Systems; 2 ug per mouse) for 4 days followed by a single i.p ...
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bioRxiv - Cancer Biology 2024Quote: ... and MMP-7 (R&D Systems) were used at 1:500.
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bioRxiv - Cancer Biology 2021Quote: ... active caspase-3 (R&D Systems AF835; 1:50), and Vimentin (CST 5741 ...
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bioRxiv - Cancer Biology 2021Quote: ... and caspase-3 (1:2000, AF835, R&D Systems) on a Ventana Benchmark Ultra automated staining system (Roche) ...
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bioRxiv - Developmental Biology 2023Quote: ... VEGFR-3 (AF349, R&D Systems, 1:100, RRID:AB_355314), Coup-TF2 (EPR18443 ...
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bioRxiv - Cell Biology 2023Quote: ... GLI-3 (AF3690, R&D Systems, dilution 1:500), RAB11FIP5 (14594-1-AP ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Galectin-3 (1:50; R&D systems, AF1197), anti-Hsp70 (1:300 ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 ng/ml IL-3 (R&D Systems) to induce cell proliferation ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were starved with 2% FBS media overnight and treated with Activin-A (R&D system, 338-AC-010, 25 ng/mL or 50 ng/mL) for indicated times.
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bioRxiv - Immunology 2023Quote: ... The remaining NK cells were transferred to a round-bottom 96-well plate and resuspended in complete RPMI supplemented with 25 ng/mL (250 IU/mL) rhIL-2 (R&D Systems, cat. no. 202-IL-010), then placed in a 37C CO2 incubator for 12-16 hours ...