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Citations for R&D Systems :
651 - 700 of 3239 citations for 14 Nonylphenoxy 3 6 9 12 tetraoxatetradecan 1 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Negative control cells were produced by treatment with 10 μM Z-DEVD-FMK caspase-3 inhibitor (R&D systems). 200μl of cell suspension from each cell line including the negative control group was transferred into a flow cytometry tube before being treated with 2.5μl of 0.2mM NucViewTM 488 caspase-3 substrate (fluorogenic DNA dye and DEVD moiety substrate for caspase-3 ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.03 to 3 μg/well of the soluble mouse or human HA-tagged recombinant fibulin 7 (R&D Systems) in 2% dry milk/TBS and 2 mM CaCl2 were added to the wells and incubated for 3 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were tested for Mycoplasma routinely every 3 months using MycoProbe Mycoplasma Detection Kit (R&D Systems).
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bioRxiv - Microbiology 2023Quote: ... coli were made from an overnight agar culture and incubated with 3 μM HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
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bioRxiv - Cancer Biology 2022Quote: ... Digested cells were washed three times with PBS and resuspended in 2/3 Cultrex Type 2 (R&D Systems) and 1/3 Full Ad-DF+++ medium and plated in drops which were allowed to solidify for 30 minutes at 37°C after which they were overlayed with Full Ad-DF+++ medium ...
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bioRxiv - Immunology 2024Quote: ... and Cl13-infected mice was measured by Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit (R&D Systems), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The mice remained on Western diet for two more weeks before euthanasia and evaluating serum concentrations of TNF-α and IL-6 via enzyme-linked immunosorbent assay (ELISA, R&D Systems, Minneapolis, MN).
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bioRxiv - Biochemistry 2022Quote: ... mice aged 6.5 weeks were starved for 6 hours and intraperitoneally injected with 220ng/g body weight of recombinant mouse FGF23 (2629-FG-025 R&D Systems, Minneapolis, MN, USA) in PBS-BSA 0.1% or PBS-BSA 0.1% vehicle with injection volume of 4μL/g body weight ...
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bioRxiv - Bioengineering 2022Quote: Transduced/Transfected and sorted hGICs harbouring either Lentivirus-MGT#1 or PiggyBac-MGT#1/4 were seeded as single cells into 6-well plates in RHB-A medium supplemented with 10ng/ml TNFa (R&D Systems, 210-TA-020) or without TNFa as control ...
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bioRxiv - Neuroscience 2022Quote: ... a complete media change was performed with CDMIII containing respective compounds at a final concentration of 8.8 ng/ml (422.6 nM) IL-6 (R&D Systems, Cat. no. 7270-IL-025) and 25 ng/ml (422.6 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded in 6 well plates and then mock-treated or treated with 5000 U/mL of human recombinant IFNβ (R&D systems, 8499-IF/CF) for 24 hours.
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bioRxiv - Cell Biology 2022Quote: ... The concentrations of IL-6 and TNF alpha in cell culture supernatants was measured by using the Mouse IL-6 and TNF alpha DuoSet ELISA (R&D systems, Minneapolis, Minnesota, USA). Statistical differences in LDH release and cytokine secretion between RAW 264.7 macrophages challenged with A ...
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bioRxiv - Immunology 2023Quote: ... the cells were plated at 0.2M cells/ml in a 6-well plate and stimulated with IFNβ (500 IU/ml, R&D systems cat. 8499-IF-010). At 24h ...
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bioRxiv - Cell Biology 2024Quote: ... The concentration of IL-6 (DY206) and VEGFA (DY293B-05) in the conditioned media was measured by using ELISA DuoSet Kit (R&D Systems, Minneapolis, MN, USA). The concentration was calculated by following the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... PNMI biomarker assays were conducted using high-sensitivity (IL-6, IL-8) and regular sensitivity (sTNF-RII, IL-1RA) ELISAs (R&D Systems, Inc., Minneapolis, MN), and raw concentrations were reported in picograms per milliliter (pg/mL).
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bioRxiv - Cell Biology 2024Quote: ... Marrow was flushed from femurs and cultured for 6 days in bone marrow medium (BMM): RPMI medium supplemented with recombinant murine CSF1 (50 ng/ml; R&D Systems, #416-ML-500), 50 mM b-mercaptoethanol ...
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bioRxiv - Cancer Biology 2024Quote: ... Some CD8+ T cells from C57BL/6 mice were directly exposed to 50ng/mL recombinant murine VEGF from R&D Systems (493-MV-005/CF) in the absence of tumor cell co-culture as a control condition ...
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bioRxiv - Physiology 2024Quote: ... rmICAM-1 (ICAM-1 Fc chimera; 15µg mL−1; R&D Systems), and rmCXCL1 (15µg mL−1 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μl of 10 mg ml-1 ubiquitin (1 mg ml-1 final) (R&D Systems, U-100H), 0.5 μl of 200 mM DTT ...
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bioRxiv - Bioengineering 2022Quote: ... angiopoietin-1 (ANG-1, R&D Systems) and hepatocyte growth factor (HGF ...
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bioRxiv - Cell Biology 2022Quote: ... NKX6-1 (1:1000; R&D Systems), NR4A2 (NURR1 ...
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bioRxiv - Genomics 2020Quote: ... YKL-40 levels were measured using a human chitinase-3 quantikine ELISA kit (R&D systems, Inc, Minneapolis, MN 20). To reduce the skewness of phenotype distributions ...
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bioRxiv - Pathology 2021Quote: ... Gdf8 and Smad2/3 proteins were quantified by ELISA methods (GDF8/Myostatin Quantikine ELISA kit, Cat # DGDF80, R&D Systems, Minneapolis ...
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bioRxiv - Microbiology 2023Quote: ... coli were made from twice passaged nutrient broth cultures and incubated with 3 uM HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
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bioRxiv - Bioengineering 2023Quote: ... and levels of tumor necrosis factor-α (TNF-α) were assessed by ELISA within 3 days (DY410; R&D Systems). The cytokines TNF-α ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell lysate from supernatant was used for IP with 3 μg of CXCL13/IgG antibody (AF801, R&D system) and 20μl precleared protein A beads (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... The following growth factors were present for the first 3 days of differentiation: 25 ng/ml BMP4 (R&D Systems), 25 ng/mL Activin A (Miltenyi Biotech ...
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bioRxiv - Cell Biology 2020Quote: Supernatant of confluent pulmonary endothelial cells were analyzed for TNF-α and IL-6 by using a mouse-specific ELISA from R&D Systems (Minneapolis, MN, USA) according to manufacturer’s protocol with the help of an Infinite M200 PRO plate reader (TECAN Instruments ...
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bioRxiv - Cell Biology 2024Quote: ... TNF-α and IL-6 release was measured using DuoSet ELISA kits according to the manufacturer’s instructions (R&D Systems: DY417-05; DY410-05; DY406-05).
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bioRxiv - Bioengineering 2023Quote: The conditioned media from treated RAW264.7s was used to quantify levels of multiple secreted cytokines/chemokines using their respective ELISA kits including IL-6 (DY406; R&D Systems Incorporated, Minneapolis, MN, USA), TNF-⍰ (DY410 ...
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bioRxiv - Developmental Biology 2022Quote: ... Jagged 1 (AF599, R&D systems, 1:500), Dlk1 (AF1144 ...
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bioRxiv - Physiology 2023Quote: ... VCAM-1 (AF643, 1:1000, R&D Systems), BNP (ab19645 ...
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bioRxiv - Developmental Biology 2024Quote: ... ESM-1 (1:100, R&D systems, AF1999), CXCR4 (1:100 ...
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bioRxiv - Developmental Biology 2020Quote: ... and the combination treatment of Tgfb1/2/3 ligands (10ng/ml, each) (R&D systems, 240-B/302-B2/243-B3). At Day2 ...
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bioRxiv - Microbiology 2021Quote: ... DY210) were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Protein levels of IFNλ from intestinal biopsies were measured by Mouse IL28B/ IFN-lambda 3 DuoSet ELISA (DY1789B, R&D Systems). Protein levels of IL-18 from intestinal biopsies were measured by ELISA (BMS618-3 ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times with wash buffer and 100 ul of samples and standards were added (R&D Systems, UK). Plates were left to incubate at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: For sTREM2 measurements, multi array 96-well plates (MSD, L15XA-3) were coated with mouse anti-TREM2 (R&D systems, AF1729) capture antibody O/N at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Definitive endoderm differentiation commenced 3-4 days post-passage onto fresh MEFs using 25 ng/ ml WNT3a (R&D Systems, UK) and 100 ng/ ml Activin-A (Peprotech ...
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bioRxiv - Microbiology 2020Quote: ... PSA was measured in lateral vaginal wall swabs using Human Kallikrein 3/PSA Quantikine ELISA kits (R&D Systems, MN, USA).
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bioRxiv - Neuroscience 2022Quote: ... The plates were washed 3 times with washing buffer and a horseradish peroxidase-streptavidin (HRP-Strep) complex (R&D Systems DY998) diluted 1:25 in reagent diluent was added to the plates and incubated for 40 minutes with agitation ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequently plates were washed 3 times with PBS-T and primary antibody (anti-RBD-mouse, R&D systems, Minneapolis, MN, USA) was added in a dilution of 1 ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were then incubated with a 5 µg/mL of human HNF-3 beta/FoxA2 antibody (R&D Systems; AF2400) for 1.5 hours at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and TNF levels were determined in the supernatants of Calu-3 and macrophage cultures using ELISA kits following the manufacturer’s instructions (R&D Systems). HMGB-1 was determined in the cell-free TA supernatants and in the supernatants of Calu-3 cultures using immunoassay following the manufacturer’s instructions (Novus Biologicals) ...
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bioRxiv - Bioengineering 2023Quote: OCI-AML5 cells were cultured in 24-well plates (3×105 cells/well) in cRPMI media (FBS from R&D Systems; Minneapolis ...
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bioRxiv - Cell Biology 2022Quote: ... Experiments were done in 293T cells pretreated with 5 ng/ml recombinant human R-spondin 3 (R&D Systems, Minneapolis, USA). Experiments were performed with biological triplicates ...
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bioRxiv - Immunology 2024Quote: ... we administered 3 doses (100 uL) of either vehicle control or 50 ug of CCL24 neutralizing antibody (MAB528, R&D systems) via intraperitoneal injection before TAC and during the first week of recovery ...
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Langerhans cells regulate immunity in adulthood by regulating postnatal dermal lymphatic developmentbioRxiv - Immunology 2024Quote: ... LCs were added at 3 x 104 per well along with anti-PIGF (10 μg/ml, R&D systems, #MAB465-500), anti-VEGFR3 (5 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... TNF-α (MTA00B) and IL-6 (M6000B) levels were determined using enzyme-linked immunosorbent assay (ELISA) kits (ELISA Development System; R&D Systems, McKinley Place, MN, USA) following the manufacturer’s protocol.75
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bioRxiv - Developmental Biology 2024Quote: ... containing 40 mM DTT (ITW Reagents, Catalog No. A2948) or cultured in 2D on plates coated with 6 μg/ml laminin (R&D Systems, Catalog No. 3446-005-01). Cultures were done in TEC medium ...