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Citations for R&D Systems :
6851 - 6900 of 10000+ citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The amount of TGF-β1 present in the media was quantified using the Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA kit(R&D systems). The results were normalized to the total number of cells in the culture at the moment of media collection.
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with affinity-purified polyclonal goat anti-huACE2 antibody recognizing the extracellular domain (1:40 dilution, #AF933, R&D Systems) for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... 25 μl of the supernatant was diluted with 25 μl 1% BSA PBS-T and added to a Nunc Maxisorp plate coated with 1 μg/ml anti-C5a capture antibody (C5a DuoSet ELISA kit, R&D systems). Next ...
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bioRxiv - Immunology 2023Quote: ... Levels of IL1β protein were determined in culture supernatant using the IL-1 beta/IL-1F2 DuoSet ELISA kit (R&D Systems) in combination with the Pierce Streptavidin-Poly-HRP (1:10,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were treated with media containing recombinant mouse transforming growth factor-beta 1 (rmTGFβ1) at 5ng/mL (R&D systems, 7666-MB) or normal media ...
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bioRxiv - Molecular Biology 2022Quote: ... Lot #: 413-ML-005) and gremlin 1 (Cat #: 956-GR-050, Lot #: ESD4616121) recombinant proteins were all purchased from R&D Systems. Gusb ...
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bioRxiv - Microbiology 2023Quote: ... and the medium was removed and washed with 1× PBS before viral infection and TGF-β1 (catalog #240-B-002, R&D Systems) treatment ...
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bioRxiv - Systems Biology 2023Quote: ... primary RTK antibodies were used at a 1:1000 concentration followed by anti-rabbit or -mouse-HRP (R&D Systems #HAF008, #HAF007). Primary antibodies p-ErbB2 (MilliporeSigma #04-293 ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then incubated in the following primary antibodies overnight at 4L: IL-17B (1:100, Cat #1248, RRID:AB_354696, R&D Systems, Minneapolis, MN), TNF-alpha (1:100 Cat #AF-410 ...
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bioRxiv - Molecular Biology 2023Quote: The cells were seeded in duplicates at 5x103 in 1 ml of mouse methylcellulose complete media without Epo (HSC008; R&D Systems) containing mIL-3 ...
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bioRxiv - Immunology 2023Quote: ... Total TGF-β levels were determined by acid activation of the latent TGF-β1 in the sample using the sample activation kit 1 (DY010) (R&D Systems).
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bioRxiv - Pathology 2023Quote: ... They were then first incubated with blocking solution for 1 hr and then with a mouse monoclonal anti-human CCR2 antibody (MAB150-SP; R&D Systems) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining volume was divided into two tubes and incubated on ice with either 1 μg of anti-galectin-9 (AF2545, R&D systems) or goat isotype control (Jackson Immunoresearch ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were then washed with FACS buffer and labeled with 1 µl/well of phycoerythrin-conjugated anti-mouse secondary (F0102B, R&D Systems) 1 hour at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556 ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation with an anti-goat IgG horseradish peroxidase-conjugated secondary antibody at a 1:1000 dilution (HAF107; R&D Systems). As a control ...
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bioRxiv - Neuroscience 2023Quote: ... Neuropili-1 inhibition studies were repeated as described above for EG00229 using a goat polyclonal anti-NRP-1 blocking antibody (AF566; R&D Systems) at 1 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... The matured THP-1 cells were not treated or treated with 20 μg/mL poly (I:C) (4287, R&D Systems, Minneapolis, MN) for four additional hours before the cells were collected for analysis.
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bioRxiv - Immunology 2023Quote: ... The culture was then diluted 1:50 in 1X PBS + 1% LB broth and incubated statically at 37°C with recombinant human SLPI protein (rSLPI) (CAS: 1274-PI-100, R&D Systems) at these final concentrations ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used in this study: Goat IgG against VE-cadherin (1:100, AF1002, R&D Systems, Minneapolis, MN), pY658 and pY685 VEC (1:40 ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was thoroughly washed with PBS containing 0.05 % Tween-20 (0.05 % PBS-T) and then incubated for 1 h at room temperature with Anti-Mouse IgG HRP-conjugated antibodies (R&D Systems), diluted 1:1000 in the blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 30 minutes in 5% Bovine Serum Albumin and incubated with primary antibodies against MRC2 (1:1,200: anti-mouse: AF4789, 1μg/mL: anti-human: AF5770; R&D Systems, Bio-Techne), SEL1L (1:2,000 ...
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bioRxiv - Microbiology 2023Quote: The levels of mouse TNF-α and IL-1β were quantified from the supernatant of homogenised skin samples using Mouse TNF-alpha DuoSet ELISA (DY410) or Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401) (R&D Systems) following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... Cytokine levels in BMEF were then measured using mouse CXCL12/SDF-1 alpha (MCX120) and SCF (MCK00) Quantikine ELISA kits (R&D Systems) according to the manufacturer’s protocols.
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bioRxiv - Cell Biology 2024Quote: ... the negative lentiviral status was confirmed for each cell line using the HIV-1 p24 DuoSet ELISA rom kit (DY7360-05, R&D Systems).
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bioRxiv - Microbiology 2024Quote: ... polyclonal), IL-12p70 (MAB611, clone 24945), IL-12p70 (MAB219, clone 24910), or MCP-1 (MAB679, clone 23007) obtained from R&D Systems. For patient sample analysis using the immunostorm chip 10 µL of diluted human plasma (1:10 dilution with 1x PBS ...
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bioRxiv - Microbiology 2024Quote: ... polyclonal), IL-6 (MAB9540, clone 973132), IL-12p70 (MAB611R, clone 24945R), and MCP-1 (AF-279, polyclonal) obtained from R&D Systems. Surface enhanced Raman scattering (SERS ...
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bioRxiv - Immunology 2024Quote: ... After overnight incubation (16-18h), cells were treated with vehicle (PBS + 1% BSA) or rmAreg (200, 500, or 1000 ng/ml) (R&D Systems). After 4h of incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... or various doses (100 ng/mL, 200 ng/mL, 400 ng/mL) of recombinant mouse IGF-1 (catalog #: 791-MG-050; R&D Systems) for 24 hours ...
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bioRxiv - Genetics 2024Quote: ... membranes were incubated with horseradish peroxidase⍰conjugated secondary antibody (hCD55 and hCD39, rabbit IgG, 1:5000 (Cat. no. HaF008, R&D Systems); hTBM and β-actin ...
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bioRxiv - Immunology 2024Quote: ... In vitro TNFα-blocking studies were performed using recombinant human TNFRII/TNFRSF1B-Fc chimera protein (1 µg / ml) (726-R2-050, R&D Systems) for 18 h.
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bioRxiv - Cell Biology 2024Quote: ... 400 µg of protein from mock and HCV-infected Huh7 cells lysed at 72 h post infection or Huh7 cells treated for 1 h with DMSO or thapsigargin were analyzed using the Proteome Profiler Human Phospho-MAPK Array (R&D Systems) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... The following antibodies were used for immunofluorescent staining during this study: mouse anti-β III-tubulin (1:500; R&D Systems, MAB1195), custom rabbit anti-JCV N (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... IL-1β protein levels were assessed using the Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN) following the manufacturer’s directions ...
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bioRxiv - Immunology 2024Quote: ... ex vivo lamina propria lymphocytes were cultured for 20 h at 37°C in complete RPMI with IL-23 (25 ng ml−1 R&D Systems) and IL-1β (20 ng ml−1 Peprotech ...
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bioRxiv - Immunology 2024Quote: ... the GR-β blot was washed 3 times with TBS-T and incubated with anti-rabbit HRP-conjugated secondary IgG (1:2000 dilution; cat. no. HAF008, R&D Systems) in 5% w/v non-fat milk (cat ...
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bioRxiv - Immunology 2024Quote: ... and CCL5 levels in the serum of mock- and MAYV-infected animals using the mouse Luminex XL cytokine assay (bio-techne) and the CCL4/MIP-1 beta DuoSet ELISA kit (Catalog# DY451-05, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with PBS and incubated with an anti-human CCR6 mouse monoclonal antibody (1:50, R&D Systems, U.S.A.) and an anti-CD4 rabbit monoclonal antibody (1:30 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were suspended in PBS containing 0.5% bovine serum albumin and a 1:10 dilution of either anti-CD31 PE conjugated flow antibody (R&D Systems #FAB3567P) or a PE-conjugated antibody isotype control (BD Pharmingen #555749) ...
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bioRxiv - Biophysics 2024Quote: ... EGFR was activated with the addition of 1 mL of pre-warmed starvation media containing 500 ng/mL EGF (R&D Systems) for 1 minute ...
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bioRxiv - Immunology 2024Quote: CCL3 and MPO concentrations were detected in immune cell supernatants after 24 h stimulation using Mouse CCL3/MIP-1 alpha DuoSet ELISA and Mouse Myeloperoxidase DuoSet ELISA kits (both R&D systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 96 well plates were coated with 1 μg/mL of recombinant IL13RA2hFc (R&D Systems, Minneapolis, MN, Cat No. 7147-IR-100) and IL13RA1hFc (R&D Systems ...
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bioRxiv - Immunology 2024Quote: Calu-3 or A549 cells were treated with 10 or 100 ng/mL of recombinant human IL-29/IFN-lambda 1 Protein (R&D Systems) for 6 or 24 hours at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... were mixed with SAv-AF647 (0.2 mg/mL, 100 μL) in PBS containing 1% bovine serum albumin (BSA, R&D Systems, MN) for 15 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Ba/F3 cell medium was additionally supplemented with 5% WEHI cell-conditioned medium as a source of IL3 while the media for IL3-independent Ba/F3 cells were supplemented or not with neuregulin-1β (NRG-1) (396-HB-050; R&D Systems). MCF10a cell medium was additionally supplemented with 20 ng/ml EGF (AF-100-15 ...
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bioRxiv - Cell Biology 2024Quote: Organoid lines were obtained as mentioned below (organoid lines) and maintained in corresponding media (see table 1) and Basement Membrane Extract (BME, R&D systems) BME®-domes ...
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bioRxiv - Cancer Biology 2024Quote: ... The GNPs were grown as described previously for our GNP proliferation conditions (1) with 3ng/ml of Shh 461-54 (R&D systems). The cells were fixed at 24 h and immunofluorescence imaging occurred ...
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bioRxiv - Pathology 2023Quote: All mouse serum samples were analyzed by a custom R&D systems Murine Premixed Multi- Analyte Kit (R&D Systems, Inc., Minneapolis, MN). The panel was run on a Luminex 200 Instrument (Luminex Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... All sections were incubated with a biotinylated secondary antibody and stained using the R&D HRP-DAB staining kit (R&D Systems, USA) and then counterstained with hematoxylin ...
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bioRxiv - Immunology 2023Quote: The two cytokines IFNγ and TNFα were detected from cell culture supernatants of the degranulation assays utilizing the respective R&D DuoSet (R&D Systems, USA) ELISAs per manufacturers’ recommendation.