Labshake search
Citations for R&D Systems :
601 - 650 of 2356 citations for Recombinant Human MAP1D His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2019Quote: ... 2013] consisting of the osteogenic media supplemented with 100 ng/mL BMP-2 (recombinant human BMP-2, 355-BM-050, R&D Systems). Media was replaced every 3 days ...
-
bioRxiv - Physiology 2020Quote: ... postnatal C57BL/6J mice at age of day 20 were intraperitoneally (I.P.) injected with recombinant human NTF3 (50μg/kg body weight, R&D Systems, Minneapolis, MN) daily for 10 days ...
-
bioRxiv - Microbiology 2021Quote: Monocyte-derived macrophages (MDMs) were obtained by stimulation of PBMC cultures with 15 ng/mL recombinant human M-CSF (R&D systems) and 10% human AB serum (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2019Quote: ... Antibodies were immobilized on CM5-Sepharose chips coated by standard amine coupling chemistry to a density of about 600 response units (RU) Measurements were carried out at 25°C by injecting 250 μl of recombinant human TNFa (R&D Systems) at concentrations from 0.2 to 50 nM with a flow rate of 35μl/min ...
-
bioRxiv - Immunology 2020Quote: ... Cells were treated at 37 °C for 30 minutes with 15 μm Forskolin and 1:3 serial dilutions of potential ligands with the following starting concentrations: 10 μM recombinant human CXCL17 (R&D Systems), 10 μM lysophosphatidic acid (LPA ...
-
bioRxiv - Immunology 2022Quote: ... coated with a recombinant form of the human P-selectin ectodomain (spanning the 42-771 sequence of the preprotein) commercially available from R&D Systems by adaptation of a published protocol (Bally et al. ...
-
bioRxiv - Systems Biology 2019Quote: ... five µg of glycopeptide in 50 mM Tris-HCl pH 7.4 was mixed with one µg recombinant human C1GalT1/C1GalT1C1 protein (R&D Systems, NM) in the presence of either 0.5 mM UDP-Gal (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2019Quote: ... EBs were subsequently transferred into 60 mm non-adhesive dishes and allowed to further differentiate in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems). After 3 days of ActA treatment EBs were plated on fibronectin (20 μg/ml ...
-
bioRxiv - Cancer Biology 2020Quote: ... with C-terminal 6xHis tag produced in HEK293 cells and human recombinant PD-L1-Fc chimera protein produced in mouse NS0 cells were obtained from R&D Systems, Minneapolis ...
-
bioRxiv - Cell Biology 2021Quote: Isolated human platelets were incubated at room temperature for 15-20 minutes with either recombinant HMGB1 (0-40 μg/ml; R&D Systems), ADP (0-5 μM ...
-
bioRxiv - Systems Biology 2019Quote: ... cells were washed with 1× Phosphate buffered saline (PBS) and stimulated with 1000 U/mL of human recombinant IFNβ (R&D Systems) in DMEM for the designated length of time ...
-
bioRxiv - Biochemistry 2019Quote: The corneal micropocket assay was performed as described16 using pellets containing 80 ng of basic fibroblast growth factor (bFGF) or 180 ng of human carrier-free recombinant vascular endothelial growth factor (VEGF; R&D Systems) in C57BL/6J mice ...
-
bioRxiv - Cancer Biology 2021Quote: Effects of rSCUBE3 were investigated by treatment of osteoclast cultures with 0.25-1.25 µg/ml recombinant human rSCUBE3 protein (Cat # 7730-SC, R&D Systems, Abingdon, UK) in the presence of RANKL and M-CSF ...
-
bioRxiv - Developmental Biology 2020Quote: ... BMP4 + Activin A treatment was accomplished by treating cells with mTeSR supplemented with 3 ng/mL recombinant human BMP4 protein (R&D Systems) and 100 ng/mL recombinant human Activin A protein (R&D Systems) ...
-
bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
-
bioRxiv - Immunology 2020Quote: ... non-tissue culture plates were coated overnight at 4゜C with recombinant human B7-H3 protein (R&D Systems, Minneapolis, MN) at 10 μg/well in 500 μL of PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... Both mouse and mixed organoids were exposed to recombinant human IL-11 (rhIL-11, R&D systems, Minneapolis, MN, USA, #218-IL) during the entire organoid culture period of 14 days.
-
bioRxiv - Cell Biology 2022Quote: Confluent T24 cells transfected with control and pacsin 2 RNAi in 6-well plates were treated with 10 ng/ml of recombinant human EGF (236-EG, R&D systems) in RPMI-1640 medium and incubated at 37℃ ...
-
bioRxiv - Immunology 2022Quote: ... 1×106 PMBCs were incubated with 20 ng of MIP-1β (recombinant human CCL4L1/MIP-1β isoform LAG-1; R&D Systems) for 30 min at 37°C ...
-
bioRxiv - Immunology 2022Quote: ... at 37°C in RPMI medium supplemented with 8% human serum and cultured for 7-9 days in the presence of 50 UI/ml of recombinant IL-2 (R&D System).
-
bioRxiv - Immunology 2022Quote: ... Recombinant human FGFs (FGF6 #238F6-025; FGF10-#345-FG-025; FGF12-2246-FG-025) were purchased from R&D systems (Minneapolis, MN).
-
bioRxiv - Cell Biology 2023Quote: ... RFP labeled eEPCs were plated with monolayer and 10ng/ml of recombinant human VEGF protein (R&D System, Cat# 293-VE-010) was treated to enhance tube formation ...
-
bioRxiv - Immunology 2022Quote: Llamas were immunized intramuscularly once per week over six weeks with carrier free recombinant human IL-9 or mouse IL-9 (R&D Systems). Each llama (4 in total ...
-
bioRxiv - Developmental Biology 2023Quote: ... EBs were transferred into 60 mm non-adhesive dishes and differentiated in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems) for 3 days ...
-
bioRxiv - Genetics 2023Quote: ... The calibrator supplied with the kit by Ansh Labs is traceable to recombinant human GDF-15 from R&D Systems (Biotechne, USA). Ansh Lab ELISA Total GDF15 between batch imprecision kit controls 7.7% at 173.2 pg/ml ...
-
bioRxiv - Cell Biology 2023Quote: M.tb-Lux was exposed to control- or HIV-ALF with or without (as control) supplemented human recombinant SP-D (5 μg/mL, R&D Systems). After exposure ...
-
bioRxiv - Immunology 2023Quote: ... The culture was then diluted 1:50 in 1X PBS + 1% LB broth and incubated statically at 37°C with recombinant human SLPI protein (rSLPI) (CAS: 1274-PI-100, R&D Systems) at these final concentrations ...
-
bioRxiv - Microbiology 2023Quote: ... This polyUb fraction was then incubated with either 1) 100 nM human recombinant PolyHistidine-otubain 1 (R&D systems, Cat# E-522B), 2 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Resting or ADP (10 μM)-stimulated platelets (4 × 103/μL) were pretreated with the inhibitor hDcR3 (10 μg/mL, recombinant human decoy receptor 3 protein, R&D Systems) for 10 min and then allowed to adhere to the coated coverslips for 60 min at RT under normoxic or hypoxic conditions ...
-
bioRxiv - Cancer Biology 2024Quote: ... GPC2 CAR expression was determined using APC or PE-conjugated human recombinant GPC2 protein (R&D Systems; 2304-GP-050; 1:500) and CD19 CAR using PE-tagged protein L (58036S ...
-
bioRxiv - Immunology 2021Quote: ... hBTLA-6×His tag (R&D systems) and hLIGHT-6×His tag proteins (made by the methods described above ...
-
bioRxiv - Biophysics 2024Quote: ... Anti-HIS antibody (R&D systems, BAM050) coated polystyrene beads were prepared as previously described.39 The beads were decorated with native purified Dsn1-6HIS-3Flag kinetochores ...
-
bioRxiv - Immunology 2021Quote: ... recombinant MBL (R&D systems) (100 µg mL-1 ...
-
bioRxiv - Bioengineering 2022Quote: ... Recombinant LOXL2 (R&D Systems) was used as a positive control.
-
bioRxiv - Neuroscience 2021Quote: ... The contribution of BDNF/TrkB signaling was tested by bath application of a scavenger of endogenous BDNF (recombinant human TrkB-Fc chimera, R&D Systems, Germany). For scavenging of BDNF ...
-
bioRxiv - Genomics 2019Quote: ... as from previous dose-response experiments3–5: recombinant human IL-1β (specific activity 1.8×107 U/mg; 201-LB-005, R&D Systems, Abingdon, UK) at 50 U/ml ...
-
bioRxiv - Cancer Biology 2021Quote: ... Fc Tag 15μg/ml (CDO-H5257, Acro Biosystem Newark, USA) or Recombinant Human IgG1 Fc 3μg/ml (110-HG, R&D Systems, Minneapolis, MN, USA) as control ...
-
bioRxiv - Immunology 2020Quote: ... Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems; R15-100). R15-100 was added every 2 to 3 days thereafter ...
-
bioRxiv - Immunology 2021Quote: The expression of NKG2D ligands in tumor cell lines was assessed using Recombinant Human NKG2D Fc Chimera Protein (R&D systems, Abingdon, UK). 105 tumor cells were incubated either with 0.5 μg of NKG2D Fc recombinant protein or IgG1-Fc during 30 min ...
-
bioRxiv - Cell Biology 2022Quote: MC3T3 cells were cultured in growth media and treated with increasing concentrations of recombinant human CXCL12/SDF-1α (R&D Systems, #350-NS) reconstituted in PBS and 0.1% BSA in a 24-well trans-well plate and allowed to migrate for 3 hours prior to cell quantification.
-
bioRxiv - Neuroscience 2023Quote: ... the cells were cultivated in neural progenitor medium based on 2D-NIM medium without the dual SMAD inhibitors SB-431542 and LDN-193189 and supplemented with 20 ng/mL recombinant human basic FGF (#233-FB, R&D Systems, Germany) and 10 µM Y-27632 (only for the first 24 h after passaging) ...
-
bioRxiv - Immunology 2023Quote: ... in PBS and HUVECs were plated at confluence and were stimulated with 40 ng/ml recombinant human TNF-α (210-TA; R&D Systems) and/or 50 ng/ml IFN-γ (285-IF ...
-
bioRxiv - Bioengineering 2023Quote: ... Plates were incubated with PBSTBA diluted 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) for 2 h at RT ...
-
bioRxiv - Physiology 2023Quote: ... ciCMVEC were placed in serum-free media 1h before treatment with 10 ng/ml of Recombinant Human TNF-α Protein (210-TA-005; R&D systems) or vehicle (PBS ...
-
bioRxiv - Genomics 2024Quote: ... 5 mM beta-glycerophosphate and 1% insulin-transferrin-selenous acid (osteogenic media) and stimulated them the next day with recombinant human BMP2 (300 ng/ml) (R&D Systems, MN) in serum-free osteogenic media ...
-
bioRxiv - Bioengineering 2024Quote: ... IDUA activity was assessed using 4-methylumberlliferyl-alpha-L-iduronide (Glycosynth, Cheshire, England) and recombinant human alpha-L-iduronidase protein (R&D systems, Minneapolis) following manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2023Quote: ... washed twice and resuspended in T-cell migration media (RPMI + 1% FBS) with or without recombinant human CXCL12 (R&D Systems, 350-NS). PBMC (50,000 ...
-
bioRxiv - Systems Biology 2023Quote: Where indicated the cells were stimulated with the final concentration of 10 ng/ml of recombinant human TNF-α protein (R&D Systems) or 100 ng/ml of recombinant human interleukin 1 beta (IL-1β ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Myc-tagged and V5-tagged CARs were stained using an Alexa Fluor 488-conjugated mouse anti-myc antibody (R&D Systems, IC3696G) and an Alexa Fluor 647-conjugated mouse anti-V5 antibody (Invitrogen ...
-
bioRxiv - Immunology 2019Quote: ... Recombinant DLL4 (#1389-D4) and recombinant Jagged-1 (#Q63722) were purchased from R&D systems and coated on plates at the same time as anti-CD3/anti-CD28 ...