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Citations for R&D Systems :
601 - 650 of 2412 citations for Recombinant Human LDLR His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Tissue constructs were cultured in the presence of 10ng/ml TGF-β3 (human recombinant active TGF-β3, R&D Systems) for the initial 14 days ...
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bioRxiv - Immunology 2024Quote: ... Cells were treated with fresh A549 media with or without human IFN-β recombinant protein (R&D Systems™ 8499IF010) at the indicated concentrations ...
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bioRxiv - Microbiology 2024Quote: Recombinant human IFN-β (8499-IF) and IFN-λ2 (1587-IL) were purchased from R&D Systems (Minneapolis, MN, USA). Both were reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2024Quote: ... and were kept overnight in complete RPMI media with 50 ng/ml of recombinant human IL-2 (R&D Systems). NK cells from HD or MM patients were stimulated in 96 wells plates with plate bound anti-CD16 (clone 3G8 Biolegend ...
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bioRxiv - Cell Biology 2024Quote: For Wnt5a experiments tetracycline induced GFP-RasV12 cells were treated with 100ng/mL recombinant human/mouse Wnt5a (R&D Systems) or PBS (control-Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... was coated with 12.5 μg/ml recombinant human N-cad Fc chimera protein (Asp160-Ala724, R&D systems, 1388-NC) or 20 μg /ml poly-D-lysine in coating buffer (HBSS with 1 mM CaCl2 ...
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bioRxiv - Biochemistry 2021Quote: Human CD38 (P28907, a soluble fragment Val43-Ile300 with 6x-His tag at C terminus) was purchased from R&D Systems (USA). It was diluted to final 0.01 mg/ml in 100 mM HEPES/NaOH buffer ...
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bioRxiv - Neuroscience 2019Quote: ... MSD GOLD 96-Well Streptavidin plates were blocked with 3% BSA and coated with a solution containing 0.25 µg/ml biotinylated polyclonal goat anti-human TREM2 capture antibody (BAF1828, R&D Systems, Minneapolis, MN). CSF and plasma samples diluted 1:4 in 1% BSA were added to the prepared plates and incubated overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Detection of bound sIL7R was carried out by one-hour incubation at room temperature with biotinylated goat anti-human IL7R polyclonal antibody (R&D Systems, # BAF306), followed by 30-minute incubation at room temperature with streptavidin-horseradish peroxidase (Millipore Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant human IL-21 was from Preprotech (200-21) and IL-21 neutralizing antibody was from R&D Systems (991-R2).
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bioRxiv - Biochemistry 2020Quote: ... and the lysate (100 μg protein) or 4 μg of recombinant human BACE1 (Cat. # 931-AS; R&D Systems, Minneapolis, MN) in extraction buffer was incubated at 37°C for 1 h with a secretase-specific peptide conjugated to two reporter molecules ...
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bioRxiv - Neuroscience 2021Quote: ... Sigma-Aldrich)-coated dishes in DMEM/F12/ B27/N2-medium supplemented with 200 ng/ml human recombinant sonic hedgehog (SHH, R&D Systems) and 100 ng/ml murine recombinant fibroblast growth factor 8b (FGF-8b ...
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bioRxiv - Cell Biology 2020Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA).
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bioRxiv - Immunology 2022Quote: Cell surface staining for the anti-HER2 BCR was performed with Recombinant Human ErbB2/Her2 Fc Chimera Protein (R&D Systems) and APC anti-His Tag antibody (Biolegend ...
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PVT1, a YAP1 dependent stress responsive lncRNA drives ovarian cancer metastasis and chemoresistancebioRxiv - Cancer Biology 2022Quote: ... epithelial to mesenchymal transition (EMT) was induced with 200 pM of Human recombinant TGF-beta 1 (R&D Systems, 240-B); verteporfin (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... media was supplemented with 5 ng/mL recombinant human TGFβ-1 (R&D Systems; resuspended in 4 mM HCl, 0.1% BSA). MCF7 ...
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bioRxiv - Immunology 2020Quote: ... ELISA was performed in 96-well plates coated with 1 μg/mL of human recombinant IL13Rα2hFc (R&D Systems, Minneapolis, MN). Following blocking with PBS/2%FBS and washes with TBS-Tween 20 buffer (Boston Bioproducts ...
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bioRxiv - Molecular Biology 2021Quote: Recombinant human transforming growth factor-β (TGF-β) and fibroblast growth factor-2 FGF-2 were purchased from R&D Systems. BTZ ...
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bioRxiv - Neuroscience 2023Quote: ... Purified recombinant NRX1β ectodomain fused to human immunogloblin Fc (NRX1β-Fc, R&D sytems, Cat#5268-NX-05, R&D system) was immobilized on carboxymethylated dextran CM5 sensor chips (Cytiva ...
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bioRxiv - Neuroscience 2022Quote: ... the media was incubated (72 h) with media containing different concentration of recombinant human PTN (rhPTN, R&D systems 252 - PL) or the drugs alectinib (Toronto Research Chemicals ...
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bioRxiv - Developmental Biology 2023Quote: ... NSPC culture in adherent and non-adherent proliferating conditions using media supplemented with human recombinant EGF and FGF2 (R&D Systems) was performed according to published protocols (Soldati et al ...
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bioRxiv - Bioengineering 2023Quote: ... Fibroblasts were treated with or without recombinant human transforming growth factor beta 1 (TGF-β1) protein (240-B, R&D Systems) at 1.25 ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... into 40 ml of R10 media supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050). Cells were passaged twice per week and feeder cells were added in a 1:1 ratio after 7 days to maintain stimulation ...
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bioRxiv - Cell Biology 2022Quote: ... Experiments were done in 293T cells pretreated with 5 ng/ml recombinant human R-spondin 3 (R&D Systems, Minneapolis, USA). Experiments were performed with biological triplicates ...
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bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL heregulin-1β (recombinant human NRG-beta 1/HRG-beta 1 EGF domain; R&D Systems, Minneapolis, MN, USA), 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1 ...
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bioRxiv - Microbiology 2024Quote: ... Monocytes were differentiated into hMDMs for at least 7 days in complete medium containing 10 ng.mL-1 of recombinant human macrophage colony-stimulating factor (rhM-CSF; R&D systems).
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bioRxiv - Cancer Biology 2020Quote: ... His (R&D Systems MAB050-100), COX2 (Abcam ab15191) ...
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bioRxiv - Cancer Biology 2020Quote: ... His (R&D Systems MAB050-100). Coverslips were incubated with the primary antibody at 4°C overnight and then washed five times for 5 minutes each in PBS following which ...
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bioRxiv - Bioengineering 2024Quote: Biotinylated anti-ITGA8 (R&D Systems, Inc.), allophycocyanin-labeled streptavidin (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant experiments were carried out by using F9 recombinant protein (R&D systems). MCF7 cell line was treated with 10 μg/ml in complete medium for two rounds of 72 hours (total of 6 days treatment) ...
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bioRxiv - Immunology 2022Quote: ... recombinant IL-21 and/or recombinant both at 25ng/ml (R&D system). On day 7 ...
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bioRxiv - Immunology 2021Quote: Five million of splenocytes per well in 12-well plates were cultured in 2.5 ml of complete DMEM medium (for details refer below) with 1000 U/ml of recombinant human (rh) IL-2 (202-IL-010, R&D Systems) at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... To induce WNT and BMP4 activation we additionally supplemented the above protocol with CHIR99021 and/or Recombinant Human BMP4 Protein (R&D Systems) (figure 3A) ...
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bioRxiv - Immunology 2022Quote: ... monocytes were cultivated for 6 days with a change of medium at day 3 in RPMI 1640/10% human serum and 10 ng/ml recombinant M-CSF (R&D Systems). At day 6 the medium was replaced with RPMI 1640/10% human serum and used for experiments on day 7.
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bioRxiv - Immunology 2019Quote: ... and resuspended for culture at 37°C + 5% CO2 for 1 h prior to stimulation with recombinant human TNFα (R&D Systems). Following stimulation ...
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bioRxiv - Bioengineering 2019Quote: ... 2013] consisting of the osteogenic media supplemented with 100 ng/mL BMP-2 (recombinant human BMP-2, 355-BM-050, R&D Systems). Media was replaced every 3 days ...
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bioRxiv - Physiology 2020Quote: ... postnatal C57BL/6J mice at age of day 20 were intraperitoneally (I.P.) injected with recombinant human NTF3 (50μg/kg body weight, R&D Systems, Minneapolis, MN) daily for 10 days ...
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bioRxiv - Microbiology 2021Quote: Monocyte-derived macrophages (MDMs) were obtained by stimulation of PBMC cultures with 15 ng/mL recombinant human M-CSF (R&D systems) and 10% human AB serum (Sigma Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies were immobilized on CM5-Sepharose chips coated by standard amine coupling chemistry to a density of about 600 response units (RU) Measurements were carried out at 25°C by injecting 250 μl of recombinant human TNFa (R&D Systems) at concentrations from 0.2 to 50 nM with a flow rate of 35μl/min ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated at 37 °C for 30 minutes with 15 μm Forskolin and 1:3 serial dilutions of potential ligands with the following starting concentrations: 10 μM recombinant human CXCL17 (R&D Systems), 10 μM lysophosphatidic acid (LPA ...
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bioRxiv - Immunology 2022Quote: ... coated with a recombinant form of the human P-selectin ectodomain (spanning the 42-771 sequence of the preprotein) commercially available from R&D Systems by adaptation of a published protocol (Bally et al. ...
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bioRxiv - Systems Biology 2019Quote: ... five µg of glycopeptide in 50 mM Tris-HCl pH 7.4 was mixed with one µg recombinant human C1GalT1/C1GalT1C1 protein (R&D Systems, NM) in the presence of either 0.5 mM UDP-Gal (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... EBs were subsequently transferred into 60 mm non-adhesive dishes and allowed to further differentiate in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems). After 3 days of ActA treatment EBs were plated on fibronectin (20 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... with C-terminal 6xHis tag produced in HEK293 cells and human recombinant PD-L1-Fc chimera protein produced in mouse NS0 cells were obtained from R&D Systems, Minneapolis ...
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bioRxiv - Cell Biology 2021Quote: Isolated human platelets were incubated at room temperature for 15-20 minutes with either recombinant HMGB1 (0-40 μg/ml; R&D Systems), ADP (0-5 μM ...
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bioRxiv - Systems Biology 2019Quote: ... cells were washed with 1× Phosphate buffered saline (PBS) and stimulated with 1000 U/mL of human recombinant IFNβ (R&D Systems) in DMEM for the designated length of time ...
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bioRxiv - Biochemistry 2019Quote: The corneal micropocket assay was performed as described16 using pellets containing 80 ng of basic fibroblast growth factor (bFGF) or 180 ng of human carrier-free recombinant vascular endothelial growth factor (VEGF; R&D Systems) in C57BL/6J mice ...
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bioRxiv - Cancer Biology 2021Quote: Effects of rSCUBE3 were investigated by treatment of osteoclast cultures with 0.25-1.25 µg/ml recombinant human rSCUBE3 protein (Cat # 7730-SC, R&D Systems, Abingdon, UK) in the presence of RANKL and M-CSF ...
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bioRxiv - Developmental Biology 2020Quote: ... BMP4 + Activin A treatment was accomplished by treating cells with mTeSR supplemented with 3 ng/mL recombinant human BMP4 protein (R&D Systems) and 100 ng/mL recombinant human Activin A protein (R&D Systems) ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...