Labshake search
Citations for R&D Systems :
601 - 650 of 1992 citations for Guanine Nucleotide Binding Protein G Protein Alpha Activating Activity Polypeptide O GNAO1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... 5 x 107 cells per sample were washed with FACS buffer (1x PBS supplemented with 2% FBS and 1 mM EDTA) and incubated with 50 µg/mL SARS-CoV-2 spike S1 subunit-Fc fusion protein (R&D systems, #10623-CV-100) or human IgG1 isotype control (BioXCell ...
-
bioRxiv - Immunology 2020Quote: Soluble proteins were quantified in EDTA anticoagulated plasma using the Luminex® multiplex platform (Luminex, Austin TX) with custom-developed reagents (R&D Systems, Minneapolis, MN), as described in detail ((16 ...
-
bioRxiv - Immunology 2022Quote: ... Recombinant Gamma (10795-CV-100) and Delta (10878-CV-100) spike proteins were purchased from R&D Systems (R&D Systems, Bio-Techne, MN, USA).
-
bioRxiv - Biophysics 2023Quote: ... The reversible osmotic stress eliminates these wrinkles and allows the conversion of effective 3D protein concentrations into 2D receptor concentrations.(37) The swelling media was supplemented with 50 nM dimeric ephrinA1-Fc (R&D Systems, #602-A1-200). The cells were allowed to equilibrate for 10 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... Aliquots of individual OCY454 WT and Cx43 KO samples differentiated for ten days were assessed for key secretory proteins implicated in osteoblast and osteoclast differentiation – sclerostin (R&D Systems; Catalog #DY1589-05) and dickkopf-related protein 1 (R&D Systems ...
-
bioRxiv - Synthetic Biology 2023Quote: ... PD-1-DNR interaction with PD-L1 was assessed by staining with 1 μg Recombinant Mouse PD-L1/B7-H1 Fc Chimera Protein (R&D Systems, 1019-B7-100), followed by anti-hFc antibody.
-
bioRxiv - Neuroscience 2020Quote: ... Full-length tau (2N4R; SP-495) and tau microtubule binding domain (MBD; SP-496) were purchased from R&D Systems. Recombinant human LRP1 cluster II ...
-
bioRxiv - Immunology 2020Quote: ... the binding was detected using 3,3’,5,5’-Tetramethylbenzidine (TMB) substrate (100 μl/well) (DuoSet ELISA Ancillary Reagent Kit, R&D Systems) as per the manufacturer’s instruction ...
-
bioRxiv - Cancer Biology 2021Quote: ... PRSS2 activity were determined with 21.5 μM Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate (R&D Systems) (final concentration ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteasome activity was also measured using 5 nM of recombinant human 26S proteasome (Cat: E-365; R&D Systems).
-
bioRxiv - Bioengineering 2021Quote: ... 20 ng/mL G-CSF (R&D Systems 214-CS), 20 ng/mL GM-CSF (R&D Systems 215-GM) ...
-
bioRxiv - Neuroscience 2023Quote: ... anti-mouse G-CSF ELISA kit (MCS00, R&D Systems), IgE ELISA kit (company?) ...
-
bioRxiv - Bioengineering 2023Quote: ... Recombinant human G-CSFR (381-GR/CF, R&D Systems) was diluted to 50 μg/mL in 10 mM acetate buffer at pH 5.0 and immobilized on the surface of a CM5 sensor chip (GE Healthcare 29149604 ...
-
bioRxiv - Neuroscience 2020Quote: ... while MABs differentiation into myotubes was initiated in the opposite compartments using MAB differentiation medium containing 2% horse serum (Cat N° 16050122) and 1% sodium pyruvate in DMEM/F12 supplemented with 0.01 μg/ml recombinant human agrin protein (R&D Systems, Cat N° 6624-AG-050). At day 21 ...
-
Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... there were six mice per treatment and control group for a total of 78 mice in thirteen groups: (A) Seven vaccine groups included 1 μg of unformulated SARS-CoV-2 spike protein (R&D Systems, Cat# 10549-CV-100) delivered with adjuvanted NPs ...
-
bioRxiv - Molecular Biology 2023Quote: ... Media supernatant was collected from the cells at the end of the experiment and IL-1β protein was analysed using an IL-1β Human Luminex® Discovery Assay kit (R&D Systems, LXSAHM; Abingdon, UK).
-
bioRxiv - Microbiology 2022Quote: ... the serum estradiol level of mice was determined using a competitive binding Estradiol Parameter Assay Kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions with 17β-estradiol as the standard ...
-
bioRxiv - Microbiology 2022Quote: The serum testosterone level of mice was determined using a competitive binding Testosterone Parameter Assay Kit (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions with testosterone as the standard ...
-
bioRxiv - Bioengineering 2023Quote: RFP-MDA-MD-231 and GFP-HUVEC at 3 million/mL and 8 million/mL in precursor solution were photopatterned and perfused using the peristaltic pump previously mentioned at 0.2 rpm/mL for five days before tumor necrosis factor-alpha (TNF-α) (R&D Systems, Minneapolis, MN, USA) and Vascular endothelial growth factor (VEGF ...
-
bioRxiv - Biophysics 2019Quote: ... Enzyme activity was confirmed in bulk solution using a fluorogenic substrate (Mca-PLAQAV-Dpa-RSSSR-NH2; R&D systems, ES003) 21 ...
-
bioRxiv - Microbiology 2021Quote: ... Dihydroxyacetone kinase was assayed using a universal kinase activity kit according to the manufacturer’s instructions (R&D systems, EA004, USA).
-
bioRxiv - Molecular Biology 2023Quote: Polystyrene microtiter plates were coated with 1 µg/ml recombinant SARS-CoV-2 spike protein produced in HEK293T cells (10549-CV-100, R&D Systems and Biotech, NE, MN, USA) while some plates were coated with 50 ng/ml SARS-CoV-2 S protein RBD or Biotinylated hACE2 (0.2 µg/ml ...
-
bioRxiv - Neuroscience 2024Quote: ... is sf21 (baculoviris)-derived mouse CX3CL1/Fractalkine protein of 34 kDa and 312 amino acids and recombinant soluble Fractalkine (R&D systems, cat. #571-MF, Accession# AAB71763) is E ...
-
bioRxiv - Immunology 2019Quote: We measured the concentrations of IL-1β and tumor necrosis factor alpha (TNF-α) in culture supernatants by using a commercial ELISA kit (R&D Systems, Minneapolis, MN, USA).
-
bioRxiv - Biochemistry 2024Quote: ... Gi-bound complex sample) or mixed with 0.5 mg/mL CXCL12 (Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha, R&D Systems Catalog #: 350-NS-050/CF), or 1 mM AMD3100 (AMD 3100 octahydrochloride ...
-
bioRxiv - Neuroscience 2020Quote: ... the superfusate was immediately collected and substance P concentration measured using a commercially-available Substance P competitive binding assay (R&D systems). All samples were run in duplicate (50 µl per sample ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cullin E3 ligase activity was blocked by supplementing egg extracts with 100 µM neddylation E1 enzyme inhibitor (MLN4924, R&D systems). Proteasome activity was inhibited via addition of 200 μM MG262 (Boston Biochem) ...
-
bioRxiv - Cancer Biology 2022Quote: In vitro PARP1 activity was measured using the HT Universal Colorimetric PARP assay kit (R&D Systems cat# 4677-096-K) and PARP2 activity was measured using the PARP2 colorimetric assay kit (BPS Bioscience cat# 80581 ...
-
bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated in the same buffer for 1 h under light agitation with the recombinant murine chimera proteins containing human Fc domains and the extracellular domains of TREM2 (R&D systems, 1729-T2-050, 0.1 mg/mL) or CD14 (R&D systems ...
-
bioRxiv - Immunology 2023Quote: ... CD4 and CD8A hispid cotton rat (Sigmodon hispidus) sequences were taken from commercially available constructs (Cotton Rat CD4 VersaClone cDNA, cat# RDC1063, R&D Systems; Cotton Rat CD8 alpha (AAL55392) VersaClone cDNA ...
-
bioRxiv - Cancer Biology 2020Quote: ... neutrophils were treated with 0.03μg/ml α-G-CSF (R&D Systems) or 50μM Chloroquine (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2020Quote: Caspase 3/7 activity was measured using a chromogenic DEVD cleavage assay according to manufacturer’s instructions (R&D Systems, Minneapolis, MN, #K106-100).
-
bioRxiv - Genomics 2019Quote: ... as from previous dose-response experiments3–5: recombinant human IL-1β (specific activity 1.8×107 U/mg; 201-LB-005, R&D Systems, Abingdon, UK) at 50 U/ml ...
-
bioRxiv - Developmental Biology 2020Quote: ... cells were treated with 1 µM SB431542 (S4317, MilliporeSigma, Burlington, MA, USA) to inhibit TGFβR1 activity or 3µM SIS3 (5291, Tocris/R&D Systems, Minneapolis, MN, USA) to inhibit SMAD3 solubilized in 50 mM DMSO ...
-
bioRxiv - Cancer Biology 2023Quote: The receptor tyrosine kinase (RTK) activities in cells after drug treatment were analyzed by a Proteome Profiler Human Phospho-RTK Array Kit (R&D Systems, ARY001B) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: PARP activity was analyzed from pulverized liver and gastrocnemius (GSN) muscle utilizing HT Colorimetric PARP/Apoptosis Assay Kit (R&D Systems, Minneapolis, MN, USA) according to manufacturer’s instructions (n=6-8 per group) ...
-
bioRxiv - Microbiology 2023Quote: ... injections with 10 µg of α-mouse G-CSF (clone: 67604, R&D Systems). Control mice received 150 µg of Rat IgG2a (clone ...
-
bioRxiv - Cell Biology 2022Quote: Proteasome activity was assessed similarly by incubating cells with genetic (7 days) or chemical (1 hour) perturbations with Me4BoVS (R&D system; I-190-050). After incubation at 37ºC for 2 hours ...
-
bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used were: Lrig1 antibody (R&D Systems, Cat. AF3688), ITGA6 antibody (clone GoH3 ...
-
bioRxiv - Biochemistry 2019Quote: ... The following antibodies were used: anti Nephrin antibody (R&D Systems), anti-HAI-1 antibody (R&D Systems) ...
-
bioRxiv - Cancer Biology 2022Quote: ... once on alternate days and/or 10 μg anti-mouse G-CSF (67604, R&D systems) every day for 8 days starting the day before chemotherapy ...
-
bioRxiv - Cell Biology 2019Quote: ... As primary antibody we used anti-MyHC antibody (R&D Systems, MAB4470) and anti-MyoG antibody (F5D supernatant from DSHB) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The antibody of α-Klotho antibody (R&D systems, MAB1819, Lot# KGN0315101) was validated for skeletal muscle histological section in our previous study(50) ...
-
bioRxiv - Microbiology 2023Quote: ... Human anti-ACE2 antibody (isotype-control antibody Goat IgG; R&D Systems) and anti-GD3 ...
-
bioRxiv - Immunology 2023Quote: ... Antibodies were anti-IL-1b antibody (BAF401, R&D Systems, 1:1,000). DRAQ5 was from Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2019Quote: ... which was either 20% autologous donor sera or 1 g/ml CXCL10 and CXCL12 (R&D Systems). Cell migration was assessed after incubation at 37°C for 4h ...
-
bioRxiv - Cancer Biology 2021Quote: ... the anti-TIM3 antibody (1:1,000, AF2365 goat monoclonal antibody, R&D Systems) was detected using the Opal 540 fluorophore (1:50 ...
-
bioRxiv - Immunology 2021Quote: ... Macrophages and neutrophils were primed where indicated with recombinant murine IFN-g (50 ng/ml, R&D Systems) for 1 hour (for neutrophil cultures ...
-
bioRxiv - Bioengineering 2023Quote: ... This was done by incubating 100 µg of recombinant human G-CSFR (381-GR/CF, R&D Systems) in 950 µL PBS for 2 h at room temperature (RT ...
-
bioRxiv - Synthetic Biology 2024Quote: ... A multiplex primary antibody mix was prepared in milk-free antibody diluent using Rabbit anti-DYKDDDK antibody (R&D Systems MAB8529; 1:1200) and mouse anti-Beta actin (R&D Systems MAB8929 ...