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Citations for R&D Systems :
601 - 650 of 947 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2022Quote: ... The transfected cells were cultured in iPSC medium with 200 ng/mL of recombinant viral B18R protein (R&D Systems). B18R was removal once the transfected cells were ready to re-seed onto irradiated MEFs.
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bioRxiv - Developmental Biology 2022Quote: ... cells were incubated in cSFDM (with RA-containing B27 supplement) with 100 ng/ml rhFGF2 (R&D Systems, #233-FB), 100 ng/ml rhFGF10 (R&D Systems ...
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bioRxiv - Genomics 2022Quote: ... National Cancer Institute Biologics Repository) for T cells and using 10 ng/mL rhTNF-α (210-TA, R&D Systems) for monocytes ...
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bioRxiv - Immunology 2023Quote: ... Cells (2E6 in 25 microliters) were stained for 90 min with anti-mouse S1PR1 (74.4 μg/ml; R&D Systems), washed twice in buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured with complete osteogenic medium mixed by human/mouse StemXVivo osteogenic/adipogenic base medium (CCM007, R&D Systems) and mouse StemXVivo ostegenic supplement (CCM009 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were plated into semi-solid methylcellulose (MethoCult™ H4435 Enriched, STEMCELL Technologies or Enriched Human Methylcellulose, R&D Systems), with further addition of 100 pg/ml rh-IL21 ...
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bioRxiv - Immunology 2023Quote: Phosphoprotein expression of CD8+ T cells was detected using a human phospho-kinase array kit (ARY003C, R&D Systems, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The strained cell pellet was resuspended in >80% ice cold Cultrex growth factor reduced BME type 2 (R&D Systems) in PDAC organoid medium ...
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bioRxiv - Immunology 2023Quote: ... IFN-beta in the cell culture supernatants was determined using the mouse IFN-beta DuoSet ELISA kit (R&D System) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: The ROSAKIT WT human cell line (RRID:CVCL_5G49) was cultured in the presence of recombinant human SCF (80 ng/mL, R&D Systems), as described previously (23) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in cold PBS containing 5.6mg/mL Cultrex Reduced Growth Factor BME (R&D Systems, 3533-010-02). Primary tumor and metastatic tumor burdens were measured weekly for 4 and 6 weeks for tail vein injection models and orthotopic models ...
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bioRxiv - Immunology 2023Quote: Cytokine levels in cell supernatants were assessed using the mouse IL-1β/IL-1F2 DuoSet ELISA kit (R&D Systems) and mouse TNF-α DuoSet ELISA kit (R&D Systems ...
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bioRxiv - Biochemistry 2023Quote: Cell lines were acquired from ATCC (HEK293T, H1299, HT1080) and tested to be free from mycoplasma (MycoProbe, R&D Systems). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2024Quote: For Wnt5a experiments tetracycline induced GFP-RasV12 cells were treated with 100ng/mL recombinant human/mouse Wnt5a (R&D Systems) or PBS (control-Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Cells were treated with fresh A549 media with or without human IFN-β recombinant protein (R&D Systems™ 8499IF010) at the indicated concentrations ...
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bioRxiv - Microbiology 2024Quote: Quantification of cytokines present in cell culture supernatants was performed by ELISA using the DuoSet ELISA kit (R&D Systems) for TNF-α ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 cells were first differentiated into macrophages with 50 nM Phorbol-12-Myristate-13-Acetate (PMA) (R&D systems) for 72 hrs ...
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bioRxiv - Biochemistry 2024Quote: The human cell line H1299 was acquired from ATCC and tested to be free from mycoplasma (MycoProbe, R&D Systems). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Molecular Biology 2020Quote: ... Tissue was permeabilized with 0.3% Triton X and quenching and blocking was performed using a cell and tissue staining kit (R&D Systems) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were starved in medium containing 0.1% FBS (mesenchymal stem cell-qualified) for 24 hours and treated with 10 ng/ml PDGF-BB (R&D systems) for 10-15 min ...
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bioRxiv - Cell Biology 2022Quote: ... Then cells were trypsinized and incubated with APC-conjugated anti-CD140b antibody (2.5 μg/ml, PR7212 clone, Mouse IgG1, R&D Systems) and FITC-conjugated anti-CD146 antibodies (5 μg/ml ...
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bioRxiv - Systems Biology 2019Quote: ... CCL2 and CCL5 secreted protein levels in cell supernatants from 1×106 murine BMDM’s was performed by ELISA (R&D Systems) according to manufactureŕs instructions.
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bioRxiv - Genomics 2019Quote: 8-week iEPs were stained with mouse E-cadherin-APC antibody (10 μL per one million cells, R&D Systems, FAB7481A) and sorted on a modified Beckman Coulter MoFlo into EcadHigh and EcadLow populations ...
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bioRxiv - Cancer Biology 2020Quote: ... PDX1 (Cell Signaling; #5679S, western blot dilution 1:1000, IHC dilution 1:100; R&D Systems; AF2517, IF dilution 1:100), NR5A2 (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... DY210) were quantified in the supernatants from uninfected and SARS-CoV-2-infected Calu-3 cells by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: CCL-149 rat lung epithelial cells (ATCC) were cultured in DMEM/F12 with 10% fetal bovine serum (FBS, R&D Systems) and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Immunology 2020Quote: ... 2 × 105 cells/ well in a 24 well plate were seeded on 10ug/ml fibronectin-coated cover slips (R&D systems) in 500ul 20ng/ml macrophage colony stimulating factor (MCSF ...
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bioRxiv - Bioengineering 2020Quote: Expression levels of cell secreted Osteoprotegerin and Vascular Endothelial Growth Factor (VEGF) was examined via ELISA (R&D Systems, Minnesota, USA). Briefly ...
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bioRxiv - Bioengineering 2019Quote: ... HUVECs were encapsulated (5·106 cells/mL) in situ within hydrogels loaded with 200 pmol/mL VEGF-165 (R&D Systems) or within non-loaded hydrogels (without GF) ...
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bioRxiv - Microbiology 2020Quote: ... cell supernatants were collected and stocked at −80°C until further analysis of IL-8 concentrations by ELISA (R&D Systems) according to manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2021Quote: ... limiting dilutions of LSK cells and their respective replicate wells were subjected to methylcellulose colony assay with HSC007 (R&D Systems) at 37°C ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: Cells were fixed with 4% paraformaldehyde followed by permeabilization and staining with primary antibodies for LOXL2 (1:500, R&D Systems) and Tetramethylrhodamine (TRITC)-conjugated Phalloidin (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were seeded on Matrigel coated culture plates in mTeSR supplemented with 10µM Y-27632 dihydrochloride (R&D Systems, 1254/10) at a density of 56,000/cm2 for routine maintenance of iPSC lines ...
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bioRxiv - Immunology 2022Quote: Cell surface staining for the anti-HER2 BCR was performed with Recombinant Human ErbB2/Her2 Fc Chimera Protein (R&D Systems) and APC anti-His Tag antibody (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... cells were divided and incubated in the presence or absence of 125 ng/ml recombinant TNF (R&D Systems, Minneapolis, MN) for 24 additional hours ...
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bioRxiv - Bioengineering 2022Quote: ... Flow cells were washed and incubated with mouse anti-human VE-cadherin (2 μg/ml clone 123413, R&D Systems, US) followed by goat anti-mouse lgG AF® 594 (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... cells were treated on either brain or vascular channel with TNF-α (Tumor Necrosis Factor-α, R&D Systems; 210-TA). The treatment was initiated after the formation of a confluent monolayer at ∼5 days in culture ...
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bioRxiv - Immunology 2020Quote: Primary murine T cells were grown in R10 media - RPMI plus 10% fetal bovine serum (FBS; R&D Systems, Minneapolis, MN) supplemented with GlutaMAX (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... the cell populations with a higher ACE2 expression in clone 43.20 were FACS sorted using ACE2-specific antibody (AF933, R&D Systems). After puromycin selection ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were centrifuged and resuspended in mTeSR1 supplemented with 10 μM SB431542 (SB; Stemgent) and 1 μM Dorsomorphin (R&D Systems). 4×106 cells were transferred to one well of a 6-well plate and kept in suspension under rotation (95 rpm ...
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bioRxiv - Neuroscience 2021Quote: Single-cell RNA-seq analysis of cultured NSCs isolated from the cortex of E14.5 Sprague-Dawley rats (NSC001, R&D systems; cultured according to the manufactures’ protocol ...
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bioRxiv - Microbiology 2020Quote: HTBE and A549 cells were seeded overnight and then treated with 100 IU/ml universal interferon beta (IFN, R&D Systems), or left untreated ...
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bioRxiv - Immunology 2021Quote: ... In-Cell ELISA was then developed using DY999 substrate solution according to the manufacturer’s recommendations (R&D Systems, Minneapolis, MN USA) and measured at 450 nm (and at 630 nm to assess the background ...
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cells were incubated with 5 µL of 25 µg/mL anti-hCD3ε (PE Conjugated Mouse IgG1, R&D System) antibody or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Cancer Biology 2021Quote: Cells were released from plates by incubating with 0.05% trypsin and then stained with primary antibody (1 μg/106 live cells) against NPY1R (MAB6400; R&D Systems) followed by secondary antibody against human IgG for 30 minutes on ice and/or with PE-conjugated ABCC5 (sc-376965 PE ...
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bioRxiv - Immunology 2022Quote: ... BAL cells were treated for 2h before infection with an ACE2 blocking antibody at 10 μg/ml (AF933, R&D systems). SARS-CoV-2 was added to the cells at MOI 0.1 or 1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Macrophage differentiation from bone marrow cells was obtained by culture in standard growth medium supplemented with m-CSF (R&D Systems) at 30 ng/ml for 7 days.
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bioRxiv - Bioengineering 2020Quote: We used the method of Cell-based ELISA with using a Human Cleaved Caspase-8 (Asp391) Immunoassay kit (R&D systems).
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bioRxiv - Immunology 2019Quote: ... and grown in B cell growth media to 80% confluence before being treated for 4h with 5μg/ml mitomycin C (Tocris, R&D Systems). Monolayers of growth arrested cells were washed 3 times with PBS ...
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bioRxiv - Developmental Biology 2019Quote: ... and were differentiated into the three different germ layers using the Human Pluripotent Stem Cell Functional Identification kit (R&D Systems) and StemMACS Trilineage Differentiation Kit (Miltenyi Biotec) ...