Hydrotris pyrazol 1 ylborato trichlorotitanium IV citations for R&D Systems

Quote: ... rabbit anti-Tuj1 polyclonal antibody (Poly18020, Covance) (1:200),and mouse anti-Tuj1 monoclonal antibody (clone TuJ-1, R&D systems) (1:500 ...

Shear flow-driven actin re-organization induces ICAM-1 nanoclustering on endothelial cells that impact T-cell migration

Izabela K. Piechocka, et al., bioRxiv - Cell Biology 2020

Quote: ... For ICAM-1 cross-linking experiments on ECs we used mouse anti ICAM-1 (Clone # BBIG-I1) from R&D systems and secondary antibody from donkey (DK) ...

Quantitative proteomics identifies secreted diagnostic biomarkers as well as tumor-dependent prognostic targets for clear cell Renal Cell Carcinoma

Aydanur Senturk, et al., bioRxiv - Cancer Biology 2021

Quote: ... Primary antibody incubation was done overnight at 4 °C with following dilutions: anti-PLOD2 mouse at 1:500-1:1,000 (MAB4445, R&D Systems), anti-FERMT3 mouse at 1:500-1:1,500 (ab173416 ...

Photostimulation of lymphatic clearance of red blood cells from the mouse brain after intraventricular hemorrhage

Dong-Yu Li, et al., bioRxiv - Neuroscience 2020

Quote: ... followed by incubation with Alexa Fluor 488-conjugated anti-Lyve-1 antibody (1:500; FAB2125G, R&D Systems, Minneapolis, Minnesota, USA), rabbit anti-Prox-1 antibody (1:500 ...

Microbiota control of maternal behavior regulates early postnatal growth of offspring

Yujung Michelle Lee, et al., bioRxiv - Physiology 2020

Quote: ... IGF-1 was measured using anti-IGF monoclonal antibody Clone #126002 and anti-mouse IGF-1 Biotinylated Antibody (R&D systems).

Adiponectin Preserves Metabolic Fitness During Aging

Na Li, et al., bioRxiv - Cell Biology 2021

Quote: ... Serum IGF-1 levels were measured by Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA). Serum parameters were measured and calculated with a VITROS analyzer (Ortho Clinical Diagnostics ...

γδ T cells are effectors of immune checkpoint blockade in mismatch repair-deficient colon cancers with antigen presentation defects

Natasja L. de Vries, et al., bioRxiv - Cancer Biology 2021

Quote: ... and stained with the different cell surface antibodies (1:10 anti-CD112-PE [clone R2.525, BD Biosciences], 1:10 anti-CD155-PE [clone 300907, R&D Systems] ...

Diet-induced obesity results in endothelial cell desensitization to VEGF-A and permanent islet vascular dysfunction

Yan Xiong, et al., bioRxiv - Physiology 2023

Quote: ... for the measurement of plasma VEGF-A and sFlt-1(Mouse VEGFR1/Flt-1 Quantikine ELISA Kit, R&D Systems, USA) levels ...

Hypothesis: Colony-forming activity of pluripotent stem cell-derived hepatocyte-like cells for stem cell assay

Kenta Ite, et al., bioRxiv - Cell Biology 2023

Quote: ... 10 ng/mL heregulin-1β (recombinant human NRG-beta 1/HRG-beta 1 EGF domain; R&D Systems, Minneapolis, MN, USA), 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1 ...

Semaglutide interferes with postnatal growth in a diet-dependent manner

Amélie Joly, et al., bioRxiv - Physiology 2024

Quote: ... supernatants were collected and used to measure IGF-1 levels by Mouse/Rat IGF-1 Quantikine ELISA (R&D systems, MG100). BCA assay was performed to normalize IGF-1 concentration to protein concentration in the liver ...

Eomes directs the formation of spatially and functionally diverse extra-embryonic hematovascular tissues

Bart Theeuwes, et al., bioRxiv - Developmental Biology 2024

Quote: ... EBs were split 1:3 in SF-D media containing recombinant human (rh) VEGF (5 ng ml-1; R&D Systems), rhBMP4 (10 ng ml-1 ...

A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes

Marion Darnaud, et al., bioRxiv - Microbiology 2021

Quote: ... Serum concentrations of IGF-1 and IGFBP-3 were determined using the Mouse/Rat IGF-1 Quantikine ELISA kit (R&D Systems) and IGFBP-3 DuoSet ELISA kit (R&D Systems) ...

Link between aging and atheroprotection in Mif-deficient atherosclerotic mice

Christine Krammer, et al., bioRxiv - Immunology 2021

Quote: ... rabbit anti-mouse Lyve1 (1:500, #DP35135P, Acris Antibody GmbH, Herford, Germany) and sheep anti-mouse Trem2 (1:50, #AF1729, R&D Systems), and rabbit anti-mouse Ki67 (1:200 ...

Spatially regulated protease activity in lymph nodes renders B cell follicles a sanctuary for retention of intact antigens

Aereas Aung, et al., bioRxiv - Immunology 2021

Quote: ... This reaction was carried out by incubating 1 μM of the quenched AZP probe with 1 μg/mL of recombinant MMP14 (918-MP-010, R&D Systems), ADAM17 (2978-AD-10 ...

Glucolipotoxicity-induced Mig6 desensitizes EGFR signaling and promotes pancreatic beta cell death

Yi-Chun Chen, et al., bioRxiv - Cell Biology 2020

Quote: ... 832/13 cells were starved for 2 h, and treated with insulin-like growth factor 1 (IGF-1, NIH repository) or hepatocyte growth factor (HGF, R&D Systems) for various times.

Involvement of denervated midbrain-derived factors in the formation of ectopic cortico-mesencephalic projection after hemispherectomy

Leechung Chang, et al., bioRxiv - Neuroscience 2021

Quote: ... The cells were immunostained with rat anti-GFP (1:2000, GF090R; Nacalai Tesque) and goat anti-TrkB (1:250, AF1494; R&D Systems). Cells were extensively washed and incubated with appropriate secondary antibodies at 4°C overnight ...

Neuronal growth regulator 1 (NEGR1) promotes synaptic targeting of glutamic acid decarboxylase 65 (GAD65)

Rui Ping Amanda Tan, et al., bioRxiv - Neuroscience 2022

Quote: Goat polyclonal antibodies against NEGR1 (AF5394) used for immunocytochemistry (IC: 1:100) and immunohistochemistry (IHC: 1:1000) were from R&D systems. Rabbit polyclonal antibodies against NEGR1 (ab101809 ...

The Modular μSiM Reconfigured: Integration of Microfluidic Capabilities to Study in vitro Barrier Tissue Models under Flow

Mehran Mansouri, et al., bioRxiv - Bioengineering 2022

Quote: ... Positive control neutrophils were incorporated into MCDB-131 Complete Media at a concentration of 900k cells.mL-1 and supplemented with 10 ng.mL-1 IL-8 (rh CXCL8/IL-8, R&D Systems, Minneapolis, MN) for 15 minutes before rinsing and stained ...

Fibrillar Aβ causes profound microglial metabolic perturbations in a novel APP knock-in mouse model

Dan Xia, et al., bioRxiv - Neuroscience 2021

Quote: ... The sandwich layout of this TREM2 MSD assay from the bottom to the top is (1) Capture-antibody (1 μg/mL; BAF1729, R&D Systems), (2 ...

A CXCL12 morphogen gradient uncovers lung endothelial heterogeneity and promotes distal vascular growth

Prashant Chandrasekaran, et al., bioRxiv - Developmental Biology 2022

Quote: ... Lung lobes were incubated with primary antibody (anti-VWF, rabbit, 1:100, F3520, Sigma and anti-EMCN, goat, 1:200, AF4666, R&D Systems), in 5% donkey serum and 0.5% Triton X-100 in phosphate buffered saline (PBST ...

Immune-mediated Tubule Atrophy Promotes Acute Kidney Injury to Chronic Kidney Disease Transition

Leyuan Xu, et al., bioRxiv - Pathology 2022

Quote: Serum KIM1 and NGAL concentrations were measured using mouse TIM-1/KIM-1/HAVCR and Lipocalin-2/NGAL quantikine ELISA kits (R&D Systems) according to the manufacturer’s instructions.

Establishing cell-intrinsic limitations in cell cycle progression controls graft growth and promotes differentiation of pancreatic endocrine cells

Lina Sui, et al., bioRxiv - Cell Biology 2020

Quote: ... cells were refreshed in DMEM + 1% (v/v) PS + 1% (v/v) B-27 + 50 ng/ml EGF (Cat. No. 236-EG, R&D System) + 25 ng/ml FGF7 for 4 days ...

Human Surfactant Protein A Alleviates SARS-CoV-2 Infectivity in Human Lung Epithelial Cells

Ikechukwu B Jacob, et al., bioRxiv - Molecular Biology 2023

Quote: ... or N-acetylglucosamine for 1 h in plates previously coated with either S protein (1 µg/ml) or RBD (50 ng/ml, VANC00B, R&D Systems). As a control ...

Characterization of CAR T Cells Manufactured using Genetically Engineered Artificial Antigen Presenting Cells

Ali Sayadmanesh, et al., bioRxiv - Immunology 2023

Quote: ... aspirated 1 ml of media and added 1 ml of CTL media containing 200 IU/ml human IL-2 (R&D Systems) to a final concentration of 100 IU/ml ...

Spike desensitisation as a mechanism for high-contrast selectivity in retinal ganglion cells

Le Chang, et al., bioRxiv - Neuroscience 2022

Quote: ... The neurofilament marker SMI32 (mouse monoclonal, 1:1000; Sternberger Monoclonals) and a goat anti-osteopontin antibody (OPN, 1:1000; R&D Systems) were used to label alpha RGCs ...

Chikungunya Virus Release is Reduced by TIM-1 Receptors Through Binding of Envelope Phosphatidylserine

Judith M. Reyes Ballista, et al., bioRxiv - Microbiology 2024

Quote: ... and treated with a blocking solution (dPBS +Ca2 +Mg2 with 2% (v/v) FBS) containing an anti-hTIM1(1:50-1:100, AF1750, R&D Systems) antibody ...

A Trisomy 21-linked Hematopoietic Gene Variant in Microglia Confers Resilience in Human iPSC Models of Alzheimer’s Disease

Mengmeng Jin, et al., bioRxiv - Neuroscience 2024

Quote: ... onto Matrigel (BD Biosciences)-coated culture plates at a ratio of 1:4∼1:8 supplemented with ROCK inhibitor Y-27632 (10 mM, R&D systems). To monitor and ensure the genetic stability of the cells ...

Matrix mechanics, not hypoxia, modulate quiescin sulfhydryl oxidase 1 (QSOX1) in pancreatic tumor cells

Catherine S. Millar-Haskell, et al., bioRxiv - Bioengineering 2022

Quote: ... A low-serum modified cell line was previously established where PANC-1 cells were cultured in DMEM supplemented with 2% FBS and 1% Insulin-Transferrin-Selenium Media Supplement (ITS, R&D Systems) (“DMEM-ITS”)45 ...

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