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Citations for R&D Systems :
5651 - 5700 of 10000+ citations for D Iditol 1 13C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... ex vivo lamina propria lymphocytes were cultured for 20 h at 37°C in complete RPMI with IL-23 (25 ng ml−1 R&D Systems) and IL-1β (20 ng ml−1 Peprotech ...
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bioRxiv - Immunology 2024Quote: ... the GR-β blot was washed 3 times with TBS-T and incubated with anti-rabbit HRP-conjugated secondary IgG (1:2000 dilution; cat. no. HAF008, R&D Systems) in 5% w/v non-fat milk (cat ...
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bioRxiv - Immunology 2024Quote: ... and CCL5 levels in the serum of mock- and MAYV-infected animals using the mouse Luminex XL cytokine assay (bio-techne) and the CCL4/MIP-1 beta DuoSet ELISA kit (Catalog# DY451-05, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed with PBS and incubated with an anti-human CCR6 mouse monoclonal antibody (1:50, R&D Systems, U.S.A.) and an anti-CD4 rabbit monoclonal antibody (1:30 ...
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bioRxiv - Immunology 2024Quote: CCL3 and MPO concentrations were detected in immune cell supernatants after 24 h stimulation using Mouse CCL3/MIP-1 alpha DuoSet ELISA and Mouse Myeloperoxidase DuoSet ELISA kits (both R&D systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 96 well plates were coated with 1 μg/mL of recombinant IL13RA2hFc (R&D Systems, Minneapolis, MN, Cat No. 7147-IR-100) and IL13RA1hFc (R&D Systems ...
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bioRxiv - Immunology 2024Quote: Calu-3 or A549 cells were treated with 10 or 100 ng/mL of recombinant human IL-29/IFN-lambda 1 Protein (R&D Systems) for 6 or 24 hours at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... or various doses (100 ng/mL, 200 ng/mL, 400 ng/mL) of recombinant mouse IGF-1 (catalog #: 791-MG-050; R&D Systems) for 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... the negative lentiviral status was confirmed for each cell line using the HIV-1 p24 DuoSet ELISA rom kit (DY7360-05, R&D Systems).
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibodies at 4 °C overnight (14-20 h) at the following dilutions: anti-PDGFRα (AF1062-SP, goat IgG, 1:250; R&D Systems), anti-APC (Ab-7 ...
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bioRxiv - Biochemistry 2024Quote: ... Secondary incubation was 1 h at ambient temperature in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, MN ...
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bioRxiv - Microbiology 2024Quote: ... polyclonal), IL-12p70 (MAB611, clone 24945), IL-12p70 (MAB219, clone 24910), or MCP-1 (MAB679, clone 23007) obtained from R&D Systems. For patient sample analysis using the immunostorm chip 10 µL of diluted human plasma (1:10 dilution with 1x PBS ...
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bioRxiv - Microbiology 2024Quote: ... polyclonal), IL-6 (MAB9540, clone 973132), IL-12p70 (MAB611R, clone 24945R), and MCP-1 (AF-279, polyclonal) obtained from R&D Systems. Surface enhanced Raman scattering (SERS ...
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bioRxiv - Immunology 2024Quote: ... After overnight incubation (16-18h), cells were treated with vehicle (PBS + 1% BSA) or rmAreg (200, 500, or 1000 ng/ml) (R&D Systems). After 4h of incubation ...
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bioRxiv - Neuroscience 2024Quote: ... IL-1β protein levels were assessed using the Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN) following the manufacturer’s directions ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were suspended in PBS containing 0.5% bovine serum albumin and a 1:10 dilution of either anti-CD31 PE conjugated flow antibody (R&D Systems #FAB3567P) or a PE-conjugated antibody isotype control (BD Pharmingen #555749) ...
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bioRxiv - Bioengineering 2024Quote: ... were mixed with SAv-AF647 (0.2 mg/mL, 100 μL) in PBS containing 1% bovine serum albumin (BSA, R&D Systems, MN) for 15 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Ba/F3 cell medium was additionally supplemented with 5% WEHI cell-conditioned medium as a source of IL3 while the media for IL3-independent Ba/F3 cells were supplemented or not with neuregulin-1β (NRG-1) (396-HB-050; R&D Systems). MCF10a cell medium was additionally supplemented with 20 ng/ml EGF (AF-100-15 ...
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bioRxiv - Biophysics 2024Quote: ... EGFR was activated with the addition of 1 mL of pre-warmed starvation media containing 500 ng/mL EGF (R&D Systems) for 1 minute ...
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bioRxiv - Cell Biology 2024Quote: Organoid lines were obtained as mentioned below (organoid lines) and maintained in corresponding media (see table 1) and Basement Membrane Extract (BME, R&D systems) BME®-domes ...
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bioRxiv - Cancer Biology 2024Quote: ... The GNPs were grown as described previously for our GNP proliferation conditions (1) with 3ng/ml of Shh 461-54 (R&D systems). The cells were fixed at 24 h and immunofluorescence imaging occurred ...
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bioRxiv - Cell Biology 2024Quote: ... media was changed to differentiation medium (DMEM [1 g/L glucose]/Ham’s F-10 supplemented with 2% FBS and 1X penicillin/streptomycin [R&D Systems; B21210]), changed daily for 5 days ...
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bioRxiv - Pathology 2023Quote: All mouse serum samples were analyzed by a custom R&D systems Murine Premixed Multi- Analyte Kit (R&D Systems, Inc., Minneapolis, MN). The panel was run on a Luminex 200 Instrument (Luminex Corporation ...
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bioRxiv - Immunology 2023Quote: The two cytokines IFNγ and TNFα were detected from cell culture supernatants of the degranulation assays utilizing the respective R&D DuoSet (R&D Systems, USA) ELISAs per manufacturers’ recommendation.
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bioRxiv - Cell Biology 2023Quote: ... All sections were incubated with a biotinylated secondary antibody and stained using the R&D HRP-DAB staining kit (R&D Systems, USA) and then counterstained with hematoxylin ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were plated at a density of 60K/well on poly-D-lysine (PDL; MilliporeSigma) and laminin (R&D Systems, Minneapolis, MN, USA)-coated coverslips in a 24-well plate ...
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bioRxiv - Neuroscience 2024Quote: ... small molecules (CHIR99021[2 mM, Axon, Groningen, Netherlands]; SB-431542[10 mM, Axon, Groningen, Netherlands]; noggin[0.5 mg/ml, R&D Systems R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2021Quote: ... and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse: R&D System 1019-B7; human: R&D System 156-B7) (indicated as anti-CD3/28-PDL1-beads ...
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bioRxiv - Immunology 2021Quote: ... and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse: R&D System 1019-B7; human: R&D System 156-B7) (indicated as anti-CD3/28-PDL1-beads ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 ng/ml Activin [cat. num. 338-AC; R&D Systems, Minneapolis, MN], 20 ng/ml FGF2 [cat. num. 233-FB-025/CF; R&D Systems, Minneapolis ...
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bioRxiv - Physiology 2022Quote: ... and were further stratified to either treatment of 5 d/wk injections of recombinant sclerostin (0.1 mg/kg body mass) (cat# 1589-ST; R&D Systems, Minneapolis, Minnesota, USA) or saline control ...
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bioRxiv - Neuroscience 2023Quote: ... Fractalkine protein levels were measured in hippocampal lysates via the R&D System’s Mouse Fractalkine (CX3CL1) Quantikine ELISA Kit (R&D Systems cat. Nr. MCX310) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Human cell line culture supernatants were analyzed using an R&D systems Human Premixed Multi-Analyte Kit (R&D Systems, Inc., Minneapolis, MN, USA) and a Luminex 200 (RRID ...
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bioRxiv - Molecular Biology 2024Quote: Soluble TNFSF14 was measured in murine and human BALF samples using commercially available ELISA kits (murine TNFSF14: R&D Systems, cat. DY1794-05, detection limit 250pg/ml, human TNFSF14: R&D Systems, cat ...
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bioRxiv - Molecular Biology 2024Quote: ... for 30 min at room temperature and then probed with the following primary and secondary antibodies: anti-Mbd4 (Santa Cruz Biotech D-6 or R&D Systems AF5935), anti-Msh6 (AbCam EPR3945) ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then incubated with the first primary antibody (an anti-IL-1R1 antibody, 1: 100, MAB269, R&D Systems, Minneapolis, USA). Negative controls were incubated in the same solution without the primary antibody ...
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bioRxiv - Bioengineering 2020Quote: ... Levels of hIFN-λ1 were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA), according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... 36 million cells were used for sorting with the Sertoli cell marker Thy-1 Cell Surface Antigen (THY1/CD90) using a PE-conjugated antibody for THY1/CD90 (R&D Systems, FAB2067P) and the EasySep™ Human PE Positive Selection Kit II (STEMCELL Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... immunosorbent 96-well plate was coated overnight at +4°C with 1 μg/ml of goat anti-mouse CDNF (R&D Systems, AF5187) in coating buffer (15mM sodium carbonate ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with FACS buffer (1x PBS supplemented with 2% FBS and 1 mM EDTA) and stained with goat anti-ACE2 (R&D Systems, #AF933) at a 1:50 dilution or rabbit anti-LRRC15 (abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... 500 000 cells were stained for 20 min at 4°C in the dark with the APC-conjugated anti-P-cad antibody (1:10; R&D system, FAB861A) and the Live/DeadTM Fixable Violet Dead Cell Stain Kit (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... PBS was removed and replaced with 200 µL of blocking solution (dPBS with 2% (v/v) BSA) containing α-TIM1(1:100, AF1750, R&D Systems) or α - AXL antibody (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 5 times with wash buffer and then incubated for 1 hour at room temperature with 200 ul of IL-1β conjugate (R&D Systems, UK). Plates were washed and incubated at room temperature for 1 hour with horseradish peroxidase-conjugated streptavidin (R&D Systems ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times with wash buffer and then incubated for 1 hour at room temperature with 200 ul of detection antibody (R&D Systems, UK). Plates were washed and incubated at room temperature for 30 minutes with horseradish peroxidase-conjugated streptavidin (strep-HRP ...
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bioRxiv - Immunology 2020Quote: ... were seeded at a concentration of 1 × l06 cells/ml in RPMI 1640 medium supplemented with 20 ng/ml GM-CSF (415-ML-050, R&D Systems, USA). After 7 d of culture ...
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bioRxiv - Microbiology 2022Quote: ... Then cells were washed three times with 1 X PBS and incubated with human ACE2 Alexa Fluor 488-conjugated antibody (R&D Systems, FAB9332G) or human TMPRSS2 Alexa-Fluor 594-conjugated antibody (R&D Systems ...
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bioRxiv - Cancer Biology 2022Quote: ... bioreactor media were either left untreated or supplemented with anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA). On day 5 ...
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bioRxiv - Immunology 2020Quote: ... and then brought to 1-2 million PBMCs/mL in R10 buffer plus 500pg/mL rhIL-7 (R&D Systems, Minneapolis, MN, USA) and rested again for four days ...
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bioRxiv - Physiology 2020Quote: ... 0.1 mL/10 g body weight) on day 1 and leptin (2 mg/kg; 0.1 mL/10 g body weight; CAT# 498-OB, R&D Systems, Minneapolis, MN) on day 2 at 6am ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were treated with 1 µM SB431542 (S4317, MilliporeSigma, Burlington, MA, USA) to inhibit TGFβR1 activity or 3µM SIS3 (5291, Tocris/R&D Systems, Minneapolis, MN, USA) to inhibit SMAD3 solubilized in 50 mM DMSO ...