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Citations for R&D Systems :
501 - 550 of 2180 citations for ETS Related Transcription Factor Elf 3 ELF3 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Intestinal organoids were grown in base culture media (Advanced DMEM/F12 media, HEPES, GlutaMax, penicillin, and streptomycin) supplemented with growth factors (EGF, Noggin, R-spondin, R&D Systems), B27 (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... bone morrow cells were cultured in Petri dishes in 10 ml DMEM cell culture medium containing 20 ng/ml of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems). On day 3 of culture ...
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bioRxiv - Microbiology 2021Quote: ... and then treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) for 7 days.
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bioRxiv - Immunology 2021Quote: ... IL-6 and tumour necrosis factor (TNF) in the supernatant were analysed by enzyme-linked immunosorbent assay (ELISA; DuoSet, R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were treated with media containing recombinant mouse transforming growth factor-beta 1 (rmTGFβ1) at 5ng/mL (R&D systems, 7666-MB) or normal media ...
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bioRxiv - Immunology 2022Quote: ... The differentiation to macrophages was initiated at day 0 by the addition of 20 ng/mL macrophage colony-stimulating factor (M-CSF, R&D Systems). Cells were cultured afterwards at 37°C and 5% CO2 for 7 days including the replacement of supplemented DMEM and replenishment of M-CSF at days 1 and 4 ...
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bioRxiv - Cell Biology 2024Quote: ... into a 96-well plate pre-coated with non-diluted Cultrex reduced growth factor base membrane extract (RGF-BME) (R&D Systems). The images of the network structure were captured by IncuCyte S3 real-time imager every 2 h at 4X magnification ...
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bioRxiv - Microbiology 2024Quote: ... and were differentiated into monocyte-derived macrophages (MDMs) by stimulation with 20 ng/mL macrophage colony-stimulating factor (M-CSF) (R&D Systems). MDMs were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Cell Biology 2024Quote: ... crypts per isolated from murine intestine and cultured in domes of Cultrex Pathclear Reduced Growth Factor Basement Membrane Extract (3533-005, R&D Systems), covered by ENR media comprised of Advanced DMEM/F12 (12634028 ...
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bioRxiv - Cell Biology 2024Quote: ... a suspension of 25,000 freshly dissociated cells or culture-expanded/FACS-sorted cells ranging from 625 to 5000 cells were prepared in growth factor reduced Matrigel (R&D Systems) and plated in 96-well plates as domes ...
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bioRxiv - Genetics 2023Quote: ... hiPSCs were plated onto Cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 25 mL flask (cat#353018 Falcon ...
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bioRxiv - Developmental Biology 2023Quote: ... washed 2x with PBS and seeded at a density 50 000 cells/50 μl reduced growth factor BME Type II (CultrexTM, R&D systems) in a 24-well cell culture plate (Corning ...
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bioRxiv - Systems Biology 2023Quote: ... on plates coated with 10 µg/cm2 Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane (R&D Systems #3434-010-02). Cells were cluster-passaged every 3-4 days with 0.5 mM EDTA and seeded into medium supplemented with 10 µM Y-27632 dihydrochloride (Bio-Techne #1254/10 ...
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bioRxiv - Neuroscience 2022Quote: ... either vehicle (PBS) or candidate factors were added at the following concentrations: BDNF (5ng/mL, R&D Systems, Cat# 248-BDB-005), GDNF (5ng/mL ...
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bioRxiv - Microbiology 2022Quote: ... Tissues were minced and digested with collagenase to single cells before embedded in reduced growth factor extracellular matrix (Cultrex BME2, R&D Systems). Selective isolation of ductal stem cells was achieved using Isolation Medium (IM ...
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bioRxiv - Developmental Biology 2023Quote: ... embryonic NSC media was prepared as above without epidermal growth factor and with the addition of 50 ng/mL mouse bone morphogenetic protein 4 (R&D Systems, 5020-BP-010 ...
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bioRxiv - Microbiology 2022Quote: ... were obtained from healthy human blood donors after preparing blood monocyte monolayers and culturing for 2 days in medium containing macrophage colony-stimulating factor (R&D Systems) as described previously (56 ...
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bioRxiv - Bioengineering 2024Quote: MDA-MB-231 spheroids were formed starting from a culture of 10,000 cells per spheroid mixed with their medium and a 2% v/v Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BioTechne, R&D systems) solution in 96-well round-bottom plates (Corning Costar Ultra-Low Attachment Multiple Well Plate) ...
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bioRxiv - Bioengineering 2024Quote: ... then differentiated into M0 macrophage with 25 ng/ml recombinant macrophage colony-stimulating factor (M-CSF) (R&D Systems, Minneapolis, MN, USA) for 4 days ...
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bioRxiv - Cancer Biology 2024Quote: Quantification of VEGF (Vascular Endothelial Growth Factor) in conditioned media and effluent was detected using a VEGF ELISA kit (Quantikine, R&D Systems) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded at 1.5×105/ml in primary differentiation medium supplemented with 1ng/ml interleukin-3 (IL-3, R&D Systems), 10ng/ml SCF and 1μg/ml doxycycline ...
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bioRxiv - Immunology 2020Quote: Cytokine and Chemokine secretion was assessed as previously described (van Wilgenburg et al., 2013) using the Proteome Profiler Human Cytokine Array Kit (R&D systems, #ARY005B). TNFα was quantified by ELISA (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Spain) and supplemented with each one of the following combinations of growth factors: (i) 100 ng/ml Act A (R&D Systems, UK), (ii ...
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bioRxiv - Microbiology 2021Quote: ... the BMMs became adherent for the osteoclastic differentiation using a complete DMEM supplemented with 50 ng/ml of receptor activator of nuclear factor kappa-B ligand (RANKL, R&D System, USA) and 20 ng/ml of M-CSF (R&D System ...
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bioRxiv - Cancer Biology 2022Quote: ... culture medium was supplemented with 0.1 to 0.5 % BME (Cultrex Reduced Growth Factor Basement Membrane Extract, type 2, Pathclear, R&D Systems, 3533-005-02). We observed batch-to-batch variations of this product ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... containing 10% fetal bovine serum and 0.5% gentamicin in the presence of 1000 IU/ml of human granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D Systems, Minneapolis, MN) and 1000 IU/ml of interleukin 4 (IL-4 ...
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bioRxiv - Microbiology 2023Quote: ... Monocyte-derived-dendritic cells (moDC) were obtained by incubation of monocytes with 1 ng/mL of granulocytes-macrophages colony-stimulating factor (GM-CSF, R&D Systems, France) and Interleukin-4 (R&D Systems ...
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bioRxiv - Microbiology 2023Quote: ... Harvested cells were cultured in RPIM 1640 medium containing 20% porcine serum and 10 ng/mL recombinant porcine granulocyte-macrophage colony-stimulating factor (GM-CSF, R&D Systems, UK) to differentiate mature bone marrow-derived macrophage (BMDMs ...
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bioRxiv - Genetics 2023Quote: Generated hiPSC lines were layered onto a cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 6-well plate with cover slips (cat#12-542-BP FisherBrand ...
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bioRxiv - Genetics 2023Quote: Fully differentiated Day 30 Cardiomyocytes were resuspended onto a cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 6-well plate with cover slips (cat#12-542-BP FisherBrand ...
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bioRxiv - Cancer Biology 2023Quote: ... the quantification of epidermal growth factor (EGF) protein levels in the serum was performed with the Mouse EGF Quantikine ELISA Kit (MEG00, R&D Systems Inc.) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2023Quote: ... and colonies were picked into a Cultrex stem cell qualified reduced growth factor basement membrane extract (cat# 3434-005-02 R&D systems Inc) coated 24-well plate for expansion ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were cultured in N2B27 with 10 ng/ml mouse basic fibroblast growth factor (bFGF) recombinant proteins (R&D systems #313FB025/CF). On Day 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... from C57BL/6 mice were differentiated from proliferating mouse bone marrow progenitors through induction with 20 ng/ml granulocyte macrophage colony stimulating factor (GM-CSF) (R&D Systems, USA) over 6–9 days as described [35] ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
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bioRxiv - Cell Biology 2024Quote: The hiPSCs were cultured in mTesRTM1 (STEMCELL 85850) on 8.68 ug/cm2 (or 0.5 mg per 6 well-plate) Cultrex reduced growth factor (RGF) (R&D Systems 3433-005-01) coated 6-well plates ...
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bioRxiv - Cell Biology 2024Quote: ... 400 µL of a 5 µg mL-1 biotinylated polyclonal Goat anti-Vascular Endothelial Growth Factor IgG solution in PBS (R&D Systems, USA) was added to CellStudio substrates containing streptavidin coated microbeads patterns (500 nm diameter beads ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with assay medium supplemented with 10 ng/ml transforming growth factor-beta 1 (hereafter referred to as TGF-β, 240-B-002, R&D Systems) for 24 h or 72 h (Peinado et al. ...
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bioRxiv - Pathology 2024Quote: ... the BMMs are incubated for three days in a 24-well plate with medium supplemented with 30 ng/ml M-CSF and 50 ng/ml receptor activator of nuclear factor kappa-B ligand (RANKL) (R&D Systems, MN). After this period ...
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bioRxiv - Microbiology 2021Quote: ... and IGFBP-3 DuoSet ELISA kit (R&D Systems), respectively ...
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bioRxiv - Developmental Biology 2020Quote: ... Rabbit α-Active Caspase-3 (R&D Systems, #AF835), Mouse α-PAX7 (DSHB) ...
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bioRxiv - Developmental Biology 2020Quote: ... supplemented with BMP4 (3 ng/ml, R&D Systems) and Thiazovivin (2 μM ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 3 μM GSK3 inhibitor (R&D Systems, CHIR99021). mESC medium was made from Knockout DMEM (Gibco 10829018) ...
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bioRxiv - Immunology 2020Quote: ... LAG-3 (Polyclonal, R&D Systems, Minneapolis, MN, USA), CD123 (clone 7G3) ...
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bioRxiv - Cancer Biology 2021Quote: ... active caspase-3 (R&D Systems AF835; 1:50), and Vimentin (CST 5741 ...
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bioRxiv - Bioengineering 2021Quote: ... 20ng/mL IL-3 (R&D Systems 203-IL), 20 ng/mL G-CSF (R&D Systems 214-CS) ...
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bioRxiv - Bioengineering 2021Quote: ... 0.01µg/mL IL-3 (R&D Systems, 203-IL) and 0.005µg/mL TNFα (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... with 20 ng/ml rmIL-3 (R&D Systems), 50 ng/ml Flt3-Ligand (R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-cleaved caspase 3 were from R&D Systems.
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bioRxiv - Cancer Biology 2021Quote: ... and caspase-3 (1:2000, AF835, R&D Systems) on a Ventana Benchmark Ultra automated staining system (Roche) ...