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Citations for R&D Systems :
501 - 550 of 1526 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... 100 ng/mL human Fms-related tyrosine kinase 3 ligand (Flt3L, #308-FK, all R&D Systems), 2 mM L-GlutaMAX (Gibco #35050-038) ...
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bioRxiv - Microbiology 2021Quote: ... organoids were washed twice with PBS and resuspended in Cultrex 3-D Culture Matrix (R&D Systems) for 30 min before organoid medium (as described above ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was quantified using Human Dkk-3 DuoSet Solid Phase Sandwich ELISA (DY1118, R&D Systems, MN), per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and the respective growth factors and small molecules (8 µM GSK-3 inhibitor CHIR99021 (R&D Systems), 200 ng/mL FGF9 (fibroblast growth factor 9 ...
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bioRxiv - Cancer Biology 2021Quote: We cultured 3*105 million freshly enriched DCs in 2ml Dendritic Cell Base Media (R&D systems) plus 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... coli were made from an overnight agar culture and incubated with 3 mM HMGB1(R&D systems) diluted with protein block buffer (Dako ...
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bioRxiv - Immunology 2023Quote: ... or with 10 µl biotinylated irrelevant antigen (3 nM final concentration; R&D Systems cat# AVI10538-050) for 20 minutes at room temperature in 96 half area white microplates (Corning cat# CLS3693) ...
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bioRxiv - Bioengineering 2024Quote: ... which was supplemented with 3 ng/mL human activin A (R&D Systems, Inc., Minneapolis, MN, USA), 1 ng/mL human bone morphogenetic protein 4 (Bmp4 ...
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bioRxiv - Developmental Biology 2022Quote: Primary antibodies used were rabbit anti-Human Phospho-Smad2/3 (R&D Systems MAB8935, 1:500 dilution), goat anti-histone (Abcam ab12079 ...
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bioRxiv - Cell Biology 2023Quote: Immunohistochemistry was performed with the following antibodies: Cleaved Caspase 3 (rabbit, R&D Systems, MAB835, 1:100), Hopx (mouse ...
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bioRxiv - Bioengineering 2024Quote: ... The FL5.12 cell culture media was also supplemented with 100 ng/ml IL-3 (R&D Systems). L1210 cells were obtained from ATCC ...
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bioRxiv - Cancer Biology 2024Quote: ... The following primary antibodies were used: rabbit anti active caspase 3 (1:200; R&D Systems AF835), and rabbit anti-Ki67 (1:5000 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were pre-incubated in 50µM of a specific caspase-3 (Z-DEVD-FMK, R&D Systems) or caspase-8 inhibitor (Z-IETD-FMK ...
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RORγt-dependent antigen-presenting cells direct regulatory T cell-mediated tolerance to food antigenbioRxiv - Immunology 2024Quote: ... cells were stimulated ex vivo for 3 h with IL-23 (10 ng/mL; R&D systems) and GolgiStop (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... cells were pre-treated with IL-4 (200 U/ml) (R&D Systems, Minneapolis, MN) for 12h at 37°C and then stimulated and infected as above ...
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bioRxiv - Cell Biology 2021Quote: ... and 4 mg/ml Collagen-I (Cultrex 3D rat tail collagen-I, R&D systems) on ice ...
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bioRxiv - Molecular Biology 2022Quote: Human recombinant BMP-2 and BMP-4 were purchase from R&D Systems (Minneapolis, MN). Anti-Myc and anti-HA antibodies were purchased from Cell Signaling Technology (Danvers ...
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bioRxiv - Neuroscience 2022Quote: ... followed by 4 hour incubation with mouse anti-O4 IgM (1:1000; R&D Systems) and rat anti-MBP (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... and 50 ng/mL bone morphogenetic protein 4 (BMP4; 314-BP, R&D Systems, USA) on day 1 ...
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bioRxiv - Neuroscience 2023Quote: ... a 10 μL intramuscular injection of Spp1 neutralizing antibody (4 μg, AF808, R&D Systems) was administered into the tibialis anterior (TA ...
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bioRxiv - Immunology 2023Quote: ... and 25ng/ml recombinant mouse IL-4 (clone 404-ML-025/CF; R&D System) in final volume of 200 μl of RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... 2.5% HEPES and stimulated with 50ng/ml IL-4 (R&D Systems Cat: 404-ML) for 15 minutes at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and MMP-7 (R&D Systems) were used at 1:500.
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bioRxiv - Microbiology 2021Quote: ... and the CD4+-enriched T cell population was cultured in AIM-V medium complemented with 40 U/mL IL-2 (Chiron) and 5 ng/mL IL-7 (R&D systems). Blood samples from HIV-1-infected patients were obtained in the context of the Biomedical Research Program TRANSBioHIV promoted by the French National Agency for AIDS and Hepatitis (ANRS ...
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bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Immunology 2022Quote: ... Monocyte differentiation into macrophages was performed for 5-7 days in the presence of human M-CSF (100 ng/ml) (R&D system). On the day of the assay ...
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bioRxiv - Immunology 2020Quote: ... ELISA for IL-7 was performed using Mouse IL-7 Quantikine ELISA Kit M700 (R&D Systems) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... cells were maintained in SFD media supplemented with the following: 3 μM CHIR 99021 (CHIR, R&D Systems), 10 ng/ml human FGF10 ...
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bioRxiv - Cell Biology 2023Quote: ... after which the cells were exposed for 3 days to 200 ng/ml FGF-9 (R&D systems) and 1μg/mL heparin (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... explants were incubated overnight in PBS 3% BSA with primary antibody: anti-Robo1 (R&D Systems, 1:400) or anti-Robo2 (gift from Dr Alain Chédotal 53 ...
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bioRxiv - Immunology 2023Quote: ... 2 ng/mL of IL-2 and 3 ng/mL of TGFβ (R&D systems, 7666-MB-055)) conditions ...
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bioRxiv - Biophysics 2020Quote: ... Purified mouse CD45RABC extracellular domain with a C-terminal 6-His tag (accession #: NP_001104786, catalog number: 114-CD-050) was purchased from R&D systems. Lectins Sambucus Nigra Lectin (SNA ...
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bioRxiv - Bioengineering 2022Quote: ... were coated with 4 μg ml-1 recombinant mouse IL-10 receptor alpha (R&D Systems) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... were coated with recombinant human CTLA-4-Fc (7268-CT, R&D Systems, Minneapolis, MN, USA) at 1 μg/mL overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 100 ng/ml Act A + 50 ng/ml bone morphogenetic protein-4 (BMP4, R&D Systems), (iv ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated with 4 µg of purified GST-tagged ISG15 (R&D systems, UL-600-500) or GSTP1 (#G5663 ...
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bioRxiv - Molecular Biology 2022Quote: ... M2 macrophages were generated by stimulating Mϕ with 20 ng/ml IL-4 (R&D Systems) and 20 ng/ml IL-13 (R&D system ...
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bioRxiv - Immunology 2020Quote: ... Supernatants were harvested after 4 h and assayed for IFN-γ via ELISA (R&D Systems).
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bioRxiv - Cell Biology 2023Quote: ... Human VEGFR3/Flt-4 antibody (1:500, AF743) was obtained from R&D systems (Minneapolis, MN). Horse Radish Peroxidase (HRP ...
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bioRxiv - Immunology 2023Quote: ... and stimulated with 0.3 μg/ml HEL plus 20 ng/ml IL-4 (R&D Systems), 0.2 μg/ml CpG (InvivoGen ...
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bioRxiv - Physiology 2024Quote: ... then stimulated with recombinant human IL-4 at 50 ng/mL (R&D Systems, Minneapolis, MN) in BEBM medium for 6 h or BEBM medium as control condition ...
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bioRxiv - Immunology 2024Quote: ... in conjunction with neutralizing antibodies against mouse IFNγ and IL-4 (also from R&D Systems). Culturing was performed in IMDM medium (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... IL-7 (5ng/ml; R&D Systems) and IL-15 (5ng/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... and rPro-MMP-7 (R&D systems) were used at 0.5 μg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... Mycoplasma testing was performed every 3 months with the MycoProbe mycoplasma detection kit from R&D systems (Minneapolis, MN). None of the cell lines used in this study are found on the misidentified cell lines list from the International Cell Line Authentication Committee.
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bioRxiv - Cancer Biology 2021Quote: ... Negative control cells were produced by treatment with 10 μM Z-DEVD-FMK caspase-3 inhibitor (R&D systems). 200μl of cell suspension from each cell line including the negative control group was transferred into a flow cytometry tube before being treated with 2.5μl of 0.2mM NucViewTM 488 caspase-3 substrate (fluorogenic DNA dye and DEVD moiety substrate for caspase-3 ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were tested for Mycoplasma routinely every 3 months using MycoProbe Mycoplasma Detection Kit (R&D Systems).
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bioRxiv - Microbiology 2023Quote: ... coli were made from an overnight agar culture and incubated with 3 μM HMGB1 recombinant protein (R&D systems) reconstituted in 1mM DTT ...
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bioRxiv - Cancer Biology 2022Quote: ... Digested cells were washed three times with PBS and resuspended in 2/3 Cultrex Type 2 (R&D Systems) and 1/3 Full Ad-DF+++ medium and plated in drops which were allowed to solidify for 30 minutes at 37°C after which they were overlayed with Full Ad-DF+++ medium ...