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• Efficient generation of lower induced Motor Neurons by coupling Ngn2 expression with developmental cues

  Francesco Limone, et al., bioRxiv - Neuroscience 2022

  Quote: ... CNTF and GDNF (R&D Systems). For seeding and culturing the first 2 days ...
• NF-κB memory coordinates transcriptional responses to dynamic inflammatory stimuli

  Andrew G. Wang, et al., bioRxiv - Systems Biology 2022

  Quote: ... mouse tumor necrosis factor alpha (TNF-α; R&D Systems, aa 80-235), mouse interleukin 1 beta (IL-1β ...
• Efficient generation of lower induced Motor Neurons by coupling Ngn2 expression with developmental cues

  Francesco Limone, et al., bioRxiv - Neuroscience 2022

  Quote: ... CNTF and GDNF (R&D Systems) and while medium in motor neuron compared contained no neurotrophic factors to start recruitment of motor neuron axons to the muscle compartment by generation of a volumetric gradient (50 μl difference in volume between the compartments ...
• NF-κB memory coordinates transcriptional responses to dynamic inflammatory stimuli

  Andrew G. Wang, et al., bioRxiv - Systems Biology 2022

  Quote: ... mouse interleukin 1 beta (IL-1β; R&D Systems, 401ML010CF), ultrapure lipopolysaccharide (LPS ...
• Efficient generation of lower induced Motor Neurons by coupling Ngn2 expression with developmental cues

  Francesco Limone, et al., bioRxiv - Neuroscience 2022

  Quote: ... CNTF and GDNF (R&D Systems). For most experiments ...
• Efficient generation of lower induced Motor Neurons by coupling Ngn2 expression with developmental cues

  Francesco Limone, et al., bioRxiv - Neuroscience 2022

  Quote: ... CNTF and GDNF (R&D Systems). For day 7 to 10 ...
• Efficient generation of lower induced Motor Neurons by coupling Ngn2 expression with developmental cues

  Francesco Limone, et al., bioRxiv - Neuroscience 2022

  Quote: ... CNTF and GDNF (R&D Systems). On day 7 ...
• Efficient generation of lower induced Motor Neurons by coupling Ngn2 expression with developmental cues

  Francesco Limone, et al., bioRxiv - Neuroscience 2022

  Quote: ... CNTF and GDNF (R&D Systems). For most experiments ...
• Hyperactivation of YAP/TAZ drives alterations in mesangial cells through stabilization of N-MYC in diabetic nephropathy

  Seunghyeok Choi, et al., bioRxiv - Pathology 2022

  Quote: ... they were incubated with anti-rabbit-HRP conjugated antibody (R&D systems, HAF 008 ...
• Hyperactivation of YAP/TAZ drives alterations in mesangial cells through stabilization of N-MYC in diabetic nephropathy

  Seunghyeok Choi, et al., bioRxiv - Pathology 2022

  Quote: ... anti-VE-cadherin (goat, polyclonal, AF1002, R&D systems), anti-ICAM2 (rat ...
• Hyperactivation of YAP/TAZ drives alterations in mesangial cells through stabilization of N-MYC in diabetic nephropathy

  Seunghyeok Choi, et al., bioRxiv - Pathology 2022

  Quote: ... anti-PDGFRβ (goat, polyclonal, AF1042, R&D systems), anti-Ki67 (rabbit ...
• Recapitulation of Human Pathophysiology and Identification of Forensic Biomarkers in a Translational Swine Model of Chlorine Inhalation Injury

  Satyanarayana Achanta, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... and lung tissue homogenates using ELISA kits (R&D Systems, Minneapolis, MN, USA).
• Anti-thrombotic treatment enhances antibiotic efficiency in a humanized model of meningococcemia

  Jean-Philippe Corre, et al., bioRxiv - Pathology 2022

  Quote: ... and PE-conjugated anti-Tissue Factor/CD142 (R&D Systems, polyclonal, #FAB3178P, 1/50).
• Dexamethasone Inhibits Cytokine-Induced, DUOX2-Related VEGF-A Expression and DNA damage in Human Pancreatic Cancer Cells and Growth of Pancreatic Cancer Xenografts

  Yongzhong Wu, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... and IFN-β (8499-IF-010) were purchased from R&D Systems (Minneapolis, MN, USA). All cytokines were dissolved in 0.1% BSA in sterilized PBS ...
• Gain-of-function, focal segmental glomerulosclerosis Trpc6 mutation minimally affects susceptibility to renal injury in several mouse models

  Brittney J. Brown, et al., bioRxiv - Physiology 2022

  Quote: ... Podocalyxin (MAB1556, R&D Systems, 1:500), TRPC6 (Alomone ACC-017 ...
• The GDF15-GFRAL pathway is dispensable for the effects of metformin on energy balance

  Anders B. Klein, et al., bioRxiv - Physiology 2022

  Quote: GDF15 was measured in human plasma or serum using the Quantikine ELISA Human GDF-15 Immunoassay (ELISA, R&D systems, catalog no. DGD150). In mice ...
• The GDF15-GFRAL pathway is dispensable for the effects of metformin on energy balance

  Anders B. Klein, et al., bioRxiv - Physiology 2022

  Quote: ... plasma samples were analyzed using Quantikine ELISA Mouse GDF15 Immunoassay (ELISA, R&D systems, catalog no. MGD150). The ELISA assays were used according to the protocol provided by the manufacturer.
• G_i/o protein–coupled receptor inhibition of beta-cell electrical excitability and insulin secretion depends on Na⁺/K⁺ ATPase activation

  Matthew T. Dickerson, et al., bioRxiv - Physiology 2022

  Quote: ... and ouabain (Oua) were purchased from R&D Systems (Minneapolis, MN). Tertiapin-Q (TPQ ...
• iPSC-based modeling of THD recapitulates disease phenotypes and reveals neuronal malformation

  Alba Tristán-Noguero, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-FOXA2 (1:50; R&D Systems), goat anti-Nanog (1:25 ...
• LuminoCell: a versatile and affordable luminometer platform for monitoring in-cell luciferase-based reporters

  Kamila Weissová, et al., bioRxiv - Molecular Biology 2022

  Quote: ... TNF-α (R&D Systems, 210-TA-005), CHIR99021 (MedChem Express ...
• iPSC-based modeling of THD recapitulates disease phenotypes and reveals neuronal malformation

  Alba Tristán-Noguero, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Nanog (1:25; R&D Systems), mouse anti-OCT3/4 (1:25 ...
• Neuronal growth regulator 1 (NEGR1) promotes synaptic targeting of glutamic acid decarboxylase 65 (GAD65)

  Rui Ping Amanda Tan, et al., bioRxiv - Neuroscience 2022

  Quote: Goat polyclonal antibodies against NEGR1 (AF5394) used for immunocytochemistry (IC: 1:100) and immunohistochemistry (IHC: 1:1000) were from R&D systems. Rabbit polyclonal antibodies against NEGR1 (ab101809 ...
• LuminoCell: a versatile and affordable luminometer platform for monitoring in-cell luciferase-based reporters

  Kamila Weissová, et al., bioRxiv - Molecular Biology 2022

  Quote: ... For cell treatment the following inhibitors and growth factors were used: FGF (R&D Systems, 233-FB-025), EGF (Sigma Aldrich ...
• SARS-CoV-2 impairs interferon production via NSP2-induced repression of mRNA translation

  Jung-Hyun Choi, et al., bioRxiv - Microbiology 2022

  Quote: ... IFN-β amounts in the culture supernatant were measured by human IFN-β ELISA kit (R&D Systems, DIFNB0) according to the manufacturer’s protocol.
• SARS-CoV-2 impairs interferon production via NSP2-induced repression of mRNA translation

  Jung-Hyun Choi, et al., bioRxiv - Microbiology 2022

  Quote: ... cells were treated with different concentrations of recombinant human IFN-β (R&D Systems, 8499-IF-010) for 12 h ...
• Glycine targets NINJ1-mediated plasma membrane rupture to provide cytoprotection

  Jazlyn P. Borges, et al., bioRxiv - Cell Biology 2022

  Quote: ... NINJ1 (R&D Systems, MAB5105) was used to determine NINJ1 expression ...
• Primary macrophages exhibit a modest inflammatory response early in SARS-CoV-2 infection

  Ziyun Zhang, et al., bioRxiv - Microbiology 2022

  Quote: ... we used a Proteome Profiler™ Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA) which contained 102 different capture antibodies that were spotted on a nitrocellulose membrane ...
• Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype

  T.M. O’Shea, et al., bioRxiv - Neuroscience 2022

  Quote: ... rat anti-Vimentin (1:500; R&D Systems, MAB2105); rat anti-Cd44 (IM7 ...
• Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype

  T.M. O’Shea, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Sox9 (1:1000, R&D systems, AF3075); goat anti-Dppa4 (1:2000 ...
• Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype

  T.M. O’Shea, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Nanog (1:1000, AF2729-SP R&D Systems); rabbit anti-Oct4 (1:1000 ...
• Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype

  T.M. O’Shea, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Carboxypeptidase E/CPE (Cpe) (1:200, R&D systems, AF3587); goat anti-Lipocalin-2 (Lcn2 ...
• Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype

  T.M. O’Shea, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Dppa4 (1:200; R&D Systems, AF3730), Rabbit anti-Id3 (1:200 ...
• Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype

  T.M. O’Shea, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Cd13 (1:200, R&D systems, AF2335); rat anti-Galectin-3 (1:200 ...
• Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype

  T.M. O’Shea, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Nestin (1:2000, R&D Systems, AF2736); rabbit alpha-smooth muscle actin (α-Sma ...
• Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype

  T.M. O’Shea, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Sox9 (1:500, R&D systems, AF3075); rabbit Aldh1l1 (1:1000 ...
• Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype

  T.M. O’Shea, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Pdgfr-α (1:500, R&D systems, AF1062); goat anti-Nestin (1:500 ...
• Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype

  T.M. O’Shea, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Clusterin (Clu) (1:200, R&D systems, AF2747); Rabbit anti-Tuj-1 (1:500 ...
• Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype

  T.M. O’Shea, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Oct4 (1:500, R&D systems, AF1759); goat anti-Sox9 (1:500 ...
• Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype

  T.M. O’Shea, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Lipocalin-2 (Lcn2) (1:200, R&D systems, AF1857), goat anti-Clusterin (Clu ...
• The interferon-inducible GTPase MxB promotes capsid disassembly and genome release of herpesviruses

  Manutea C. Serrero, et al., bioRxiv - Microbiology 2022

  Quote: ... The cells were cultured with 1000 U/mL human type I IFN-α2a (MφIFN; R&D Systems, Minneapolis, Minnesota, USA) or left untreated for 16 h.
• Discovery and functional interrogation of SARS-CoV-2 protein-RNA interactions

  Joy S. Xiang, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Y-27632 (R&D Systems) on day 1 then only activin A on days 2 and 3 ...
• Discovery and functional interrogation of SARS-CoV-2 protein-RNA interactions

  Joy S. Xiang, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Recombinant His-tagged NSP12 (R&D Systems, catalog # 10686-CV) was incubated with in vitro transcribed and biotinylated RNA in 20 mM Tris ...
• Phosphatidic acid inhibits inositol synthesis by inducing nuclear translocation of IP6K1 and repression of myo-inositol-3-P synthase

  Pablo Lazcano, et al., bioRxiv - Molecular Biology 2022

  Quote: ... supplemented with 10% FBS (R&D Systems) and 1% penicillin-streptomycin (Gibco) ...
• Neuronal membrane proteasomes homeostatically regulate neural circuit activity in vivo and are required for learning-induced behavioral plasticity

  Hai-yan He, et al., bioRxiv - Neuroscience 2022

  Quote: ... and Bicuculline Methiodide (BMI, R&D systems, Cat# 2503, 25∼50μM) were injected into the midbrain ventricle of st48 tadpoles ...
• Reactive astrocytes transduce blood-brain barrier dysfunction through a TNFα-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin

  Hyosung Kim, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-VE-cadherin (1:300 dilution, AF938, R&D Systems). The following day ...
• Reactive astrocytes transduce blood-brain barrier dysfunction through a TNFα-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin

  Hyosung Kim, et al., bioRxiv - Neuroscience 2022

  Quote: ... E-Selectin (AF724, R&D systems, 1:1000 dilution), and GAPDH (D16H11 ...
• Contribution of the Wolffian duct mesenchyme to the formation of the female reproductive tract

  Fei Zhao, et al., bioRxiv - Developmental Biology 2022

  Quote: ... tissues were cultured for another 48h with or without IGF1 (291-G1-200, R&D system). After culture ...
• Directed differentiation of EA/TEF patient-derived induced pluripotent stem cells into esophageal epithelial organoids reveal SOX2 dysregulation at the anterior foregut stage

  Suleen Raad, et al., bioRxiv - Developmental Biology 2022

  Quote: ... 10ng/mL human FGF7 (R&D systems, US. Catalog# 251-KG-010/CF), 10ng/mL BMP4 (Peprotech ...
• Directed differentiation of EA/TEF patient-derived induced pluripotent stem cells into esophageal epithelial organoids reveal SOX2 dysregulation at the anterior foregut stage

  Suleen Raad, et al., bioRxiv - Developmental Biology 2022

  Quote: ... 10ng/mL human FGF10 (R&D systems, US. Catalog# 345-FG-025/CF), 10ng/mL human FGF7 (R&D systems ...
• Stable iPSC-derived NKX2-1⁺ Lung Bud Tip Progenitor Organoids Give Rise to Airway and Alveolar Cell Types

  Renee F.C. Hein, et al., bioRxiv - Developmental Biology 2022

  Quote: ... SFB-VA is supplemented with 10ng/mL FGF7 (R&D Systems, Cat#251-KG/CF), 3µM CHIR99021 (APExBIO ...