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Citations for R&D Systems :
451 - 500 of 512 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 1 x 107 cells were added to 100mm Petri dishes containing 15 ml of complete RPMI media plus 200 to 1000 U/ml IFN-β (R&D Systems 8234MB010). All cells were grown in a 37⁰C incubator with 7.5% CO2 for 5 h to 4 days (as indicated ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were maintained in serum-starved media with or without 10ng/ml of recombinant human TGF-β1 (R&D Systems, cat 240-B/CF) for 48 hours and harvested ...
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bioRxiv - Immunology 2022Quote: ... were cultured in OptiMEM reduced serum media buffered with sodium bicarbonate and supplemented with 4% (v/v) fetal bovine serum (FBS, R&D Systems, Minneapolis, MN). The cells were grown at 37 °C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Neuroscience 2023Quote: ... On days 27 to 40 organoids were fed on alternate days with media containing 20 ng/mL NT-3 (R&D Systems, 267-N3) and 10 ng/mL BDNF (R&D Systems 248-BD) ...
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bioRxiv - Bioengineering 2022Quote: ... was determined using the dot blot-based mouse XL cytokine array kit for the model group breast tissues and human XL cytokine array kit and human XL oncology array kit for the human cell conditioned media following manufacturer’s instructions (R&D Systems, Cat. No: ARY028). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: The concentration of PEDF was measured in the conditioned media collected from control and knockdown QGY-7703 cells by using ELISA kit (R&D Systems, Minnesota, USA) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... the cell media were exchanged with 400 µL ice-cold DMEM media containing 10 % FBS and 1 µg/mL α-TrkB antibody (goat anti human/mouse TrkB, R&D Systems #AF1494). The cells were incubated on ice for 2 hours ...
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bioRxiv - Immunology 2024Quote: ... and the adherent irradiated 40LB cells were co-cultured with 5 × 104 naïve splenic B cells in 6 mL of B cell media containing IL-4 (R&D Systems, 1 ng/mL) for 96 hours ...
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bioRxiv - Bioengineering 2024Quote: ... agarose beads (100-200 mesh Affi-Gel Blue Media, Bio Rad; Hercules, CA) containing exogeneous FGF-10 (345-FG, R&D Systems; Minneapolis, MN) were implanted within the pulmonary mesenchyme along regions of the primary bronchus that do not normally branch ...
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bioRxiv - Neuroscience 2020Quote: ... in DMEM/F-12 and plated into either 60 mm or 100 mm tissue culture dishes as described above with the addition of rmIL-1α or rmIL-1β (50 or 500 pg/mL, R&D Systems, Minneapolis, MN)) for treatment groups ...
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bioRxiv - Developmental Biology 2021Quote: Meiosis was induced after 3 days of mitotic expansion culture as previously reported (Miyauchi et al, 2017, 2018) by a combined treatment of 300 ng/ml BMP2 (R&D Systems, 355-BM) and 100 nM RA ...
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bioRxiv - Physiology 2021Quote: ... primary osteoclast precursors were seeded after initial culture on non-treated plates into 6 well plates in the presence of 30ng/mL recombinant mouse M-CSF (R&D systems, Minneapolis, MN) for 3 days ...
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bioRxiv - Immunology 2020Quote: ... and IL-10 were measured in the cell-free supernatants of control and treated MoDCs cultures (4×105 cells/ml) by ELISA assays (R&D Systems, Minneapolis, USA). The levels of cytokines produced by CD4+ T lymphocytes in co-cultures with MoDCs were evaluated in the cell-free supernatants of co-cultures ...
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bioRxiv - Bioengineering 2024Quote: CHO cells were routinely cultured in 10-cm tissue culture grade plates using high-glucose DMEM supplemented with 10 % fetal bovine serum (R&D systems, Bio-Techne), 110 µg/mL sodium pyruvate ...
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bioRxiv - Immunology 2024Quote: Supernatants harvested from mixed lymphocyte cultures established from three mice of each group were tested using commercial assay kits (e.Bioscience, R&D Systems, Bender MD Systems) for the presence of interferon-γ ...
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in appropriate cell culture medium as directed by supplier with 10% heat-inactivated fetal bovine serum (FBS) (R&D Systems; Minneapolis, MN) and 1% Penicillin-Streptomycin (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: Human cell line culture supernatants were analyzed using an R&D systems Human Premixed Multi-Analyte Kit (R&D Systems, Inc., Minneapolis, MN, USA) and a Luminex 200 (RRID ...
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bioRxiv - Microbiology 2024Quote: Secreted IL-1β or IP-10 in culture supernatants was measured using a commercially available Human IL-1 beta/IL-1F2 DuoSet ELISA kit (R&D Systems, catalog #DY201) or Human IP-10 ELISA Set (BD Biosciences) ...
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bioRxiv - Bioengineering 2024Quote: ... was used as a hydrogel matrix for BxPC3 cell culture and Cultrex Reduced Growth Factor Basement Membrane Extract (BME) (R&D Systems, BME001-05) were used as the hydrogel matrix for A549 ...
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bioRxiv - Cancer Biology 2024Quote: Protein profiling of the secreted factors from each cell culture was performed using the Proteome Profiler Human XL Cytokine array kit (R&D systems, Minneapolis, MN) according to the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2020Quote: ... the EBs were dissociated with trypsin-EDTA and reaggregated for 40 hr in serum-free differentiation media with the addition of 8ng/ml human activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R & D Systems Cat# 314-BP) ...
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bioRxiv - Cancer Biology 2020Quote: ... CHLA9 and CHLA10 cells were cultured in IMDM +L-glutamine media supplemented with 20% FBS and 1% insulin-selenium-transferrin (ITS, R&D Systems, cat no: AR013). Cell lines were maintained at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... For differentiation assays cells were cultured in differentiation media (DMEM + 1% FBS + 1% P/S) with or without recombinant TGFβ (R&D systems, 10 ng/mL) and analyzed 72 hours later by immunofluorescent staining described below ...
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bioRxiv - Cancer Biology 2021Quote: ... the culture media were replaced with 1 ml fresh media supplemented with 10% FBS and the designated concentration of the recombinant mouse IFNγ (R&D Systems, 485-MI-100) or recombinant human IFNγ (R&D Systems ...
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bioRxiv - Biophysics 2023Quote: ... The reversible osmotic stress eliminates these wrinkles and allows the conversion of effective 3D protein concentrations into 2D receptor concentrations.(37) The swelling media was supplemented with 50 nM dimeric ephrinA1-Fc (R&D Systems, #602-A1-200). The cells were allowed to equilibrate for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... BMSCs were plated in T-75 flasks overnight with standard OCY454 media supplemented with 10ng/ml M-CSF (R&D Systems Catalog #416-ML-010) at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... osteoclast differentiation was initiated using αMEM complete media supplemented with 30 ng/mL M-CSF and 100 ng/mL RANKL (R&D Systems, 462-TEC/CF)(57) ...
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bioRxiv - Molecular Biology 2023Quote: ... according to the manufacturer’s protocol and reaggregated for 40h in serum-free differentiation media with the addition of 8 ng/ml human Activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R&D Systems Cat# 314-BP) ...
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bioRxiv - Bioengineering 2023Quote: ... Human mesenchymal stem cells (Passage 5-7) (commercially obtained from Rooster Bio (Frederick, MD)) were cultured in hMSC growth media (R&D Systems, Minneapolis, MN, USA). Adipose-derived stem cells (Passage 3-5 ...
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bioRxiv - Immunology 2022Quote: ... To assess the cytokine profile of the different conditions plasma and PBMC conditioned media from 3 donors were pooled separately and subjected to the Proteome Profiler Assay Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA). The assay was performed according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... They were then perfused through the renal vein at 100 mL/min with a pressure less than 20 mmHg in custom antibiotic-free endothelial media containing endothelial basemedia (R&D Systems, Minneapolis, MN, #CUST01707), sodium bicarbonate (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... Conditioned media was collected 24 hours later and stored at -80 °C until analysis of TNF-⍰ levels via ELISA (DY210; R&D Systems, Minneapolis, MN, USA).
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bioRxiv - Cell Biology 2024Quote: ... The concentration of IL-6 (DY206) and VEGFA (DY293B-05) in the conditioned media was measured by using ELISA DuoSet Kit (R&D Systems, Minneapolis, MN, USA). The concentration was calculated by following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... Maturation of hepatic endoderm was initiated on differentiation days 16 ─ 21 using complete HCM media supplemented with 10ng/ml Oncostatin M (OSM: R&D systems, # 8475-OM-050), 20ng/ml HGF and 0.1μM Dexamethasone (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... 1 × 105 B cells were added to each well in 6 mL of B cell media containing either IL-4 (R&D Systems, 1 ng/mL) or IL-21 (R&D Systems ...
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bioRxiv - Developmental Biology 2024Quote: ... the embryoid bodies (EB) were harvested and seeded in serum-free differentiation media supplemented with 1 ng/ml human Activin A (R&D Systems Cat#. 338-AC) and 1 ng/ml human BMP4 (R&D Systems Cat# 314-BP) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cytokines released from HCEC-1CT cells into cell culture supernatants after 5 h of incubation were measured using a Magnetic Luminex® assay (R&D SYSTEMS, Minneapolis, USA). Cell culture supernatant samples were collected after the incubation for cytokine gene transcription (details for incubation see 2.4. ...
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bioRxiv - Immunology 2022Quote: The cytokine/chemokine profile of mice plasma or culture supernatants was determined by using proteome profiler mouse cytokine array kit (R&D Systems, CAT. No. ARY006). The expression levels of cytokine/chemokine were measured by pixel density of each dot using the ImageJ software ...
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bioRxiv - Neuroscience 2023Quote: ... CD8 T cells were activated with immunoCult Human CD3/CD28/CD2 T Cell Activator beads (cat. #10970) in co-culture with NK cells in the presence of IL2 (2ng/mL) (R&D Systems cat. #BT-002) and IL15 (0.1ng/mL ...
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bioRxiv - Immunology 2023Quote: Cytokines in the patient and control dermal fibroblast culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) for quantitative detection of IL-1β (R&D Systems Cat. No. DY201), tumour necrosis factor TNF-α (BioLegend® Cat ...
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bioRxiv - Genomics 2024Quote: ... The human RPTEC with early passages were seeded at 8 × 105 cells on 10 cm culture dish at the day before the assay and treated with or without TNFα (R&D systems, 100 ng/ml) at 3 h before the fixation ...
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bioRxiv - Microbiology 2023Quote: ... The levels of CD83 (pg/ml) secreted in the culture medium were determined with the Human CD83 DuoSet ELISA kit (R&D Systems, Minneapolis, Minnesota, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The concentrations of IL-6 and TNF alpha in cell culture supernatants was measured by using the Mouse IL-6 and TNF alpha DuoSet ELISA (R&D systems, Minneapolis, Minnesota, USA). Statistical differences in LDH release and cytokine secretion between RAW 264.7 macrophages challenged with A ...
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bioRxiv - Cancer Biology 2024Quote: Transparent PET membrane culture inserts of 24-well plate (Falcon, 353097) were coated with Cultrex Reduced Growth Factor Basement Membrane Extract (BME) (R&D Systems, 3533-010-02) at 50μg per membrane (200μl of 0.25mg/ml BME stock per membrane ...
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bioRxiv - Immunology 2024Quote: ... The analysis of MICA concentration in cell culture supernatant was conducted according to the instructions of Human MICA ELISA kit (R&D systems, catalog no. DY1300).
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bioRxiv - Immunology 2024Quote: Cytokines were studied using the supernatant from tissue culture and a sandwich ELISA kit from Bio-Techne for TNF-α (R&D Systems Cat. DY410), IL-1β (R&D Systems Cat ...
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bioRxiv - Neuroscience 2021Quote: ... Media was then exchanged to DMEM containing 15 ng/mL recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF; R&D Systems # 215-GM-010/CF). PBMC Mϕ were allowed to differentiate for an additional 5 days and were returned to DMEM 10% FBS until stimulation.
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bioRxiv - Immunology 2024Quote: ... 300 μL of each 1:10 dilution mix (containing cells and ADC) was added to 3mL aliquots of complete mouse or human methylcellulose media (R&D Systems; HSC007 and HSC005, respectively) and vortexed thoroughly ...
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bioRxiv - Bioengineering 2024Quote: ... Organoid basal growth media was supplemented with epidermal growth factor (EGF) (100 ng/mL, 10 ng/mL, 1 ng/mL R&D Systems Cat#236-EG-01M) or Epiregulin (EREG ...
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bioRxiv - Immunology 2022Quote: IL-10 and TNF-α levels were measured in the cell-free supernatants of cell cultures using a cytokine Duo-Set Kit (R&D Systems, Minneapolis, MI, USA), according the manufacturer’s instructions.