Labshake search
Citations for R&D Systems :
451 - 500 of 555 citations for Phospho Tyrosine Rabbit Polyclonal HRP labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2024Quote: ... CHO-derived rhBMP2 standard and streptavidin-HRP working solution from the hBMP2 DuoSet ELISA system (DY355, R&D systems) were used for all experiments ...
-
bioRxiv - Pathology 2022Quote: ... No. P0260, and goat anti-rabbit, Cat. No. P0448, from DAKO and rabbit anti-goat, Cat. No. HAF017, from R&D Systems) were used with a dilution 1:2500 ...
-
bioRxiv - Cell Biology 2019Quote: ... slides were blocked and stained using rabbit anti-LOXL2 (Gil25-70, Gilead Sciences) antibody overnight at 4° C and a rabbit cell & tissue staining kit (R&D Systems) as recommended by the manufacturer ...
-
bioRxiv - Immunology 2021Quote: ... the same protein was labeled using its His-tag by incubating the antigen with an Alexa Fluor 647-conjugated anti-His-antibody (R&D Systems, IC0501R) for 30 minutes at room temperature at a 2:1 ratio (RBD molecules:IgG molecules) ...
-
bioRxiv - Developmental Biology 2020Quote: ... Rabbit α-Active Caspase-3 (R&D Systems, #AF835), Mouse α-PAX7 (DSHB) ...
-
bioRxiv - Cell Biology 2022Quote: ... rabbit anti-BRG1 (1:250, R&D systems, MAB5738), mouse anti-FOXM1 (1:100 ...
-
bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Caspase3 (1:1000, R&D Systems, AF835), goat anti-Foxp2 (1:1000 ...
-
bioRxiv - Developmental Biology 2022Quote: ... BRACHYURY (rabbit monoclonal, R&D Systems MAB20851, 1:400), EOMES (mouse monoclonal ...
-
bioRxiv - Cancer Biology 2021Quote: ... rabbit monoclonal anti-P-DDR2 (MAB25382, R&D Systems), mouse monoclonal anti-P-Erk (9106S ...
-
bioRxiv - Neuroscience 2019Quote: ... MSD GOLD 96-Well Streptavidin plates were blocked with 3% BSA and coated with a solution containing 0.25 µg/ml biotinylated polyclonal goat anti-human TREM2 capture antibody (BAF1828, R&D Systems, Minneapolis, MN). CSF and plasma samples diluted 1:4 in 1% BSA were added to the prepared plates and incubated overnight at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... Detection of bound sIL7R was carried out by one-hour incubation at room temperature with biotinylated goat anti-human IL7R polyclonal antibody (R&D Systems, # BAF306), followed by 30-minute incubation at room temperature with streptavidin-horseradish peroxidase (Millipore Sigma ...
-
bioRxiv - Immunology 2020Quote: ... paraformaldehyde-fixed frozen sections were treated with citrate buffer (pH 7.0, 121°C, 5 min) before immunostaining with sheep anti-mouse Spi-B polyclonal Ab (R&D Systems, Abingdon, UK). CCL20 ...
-
bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed 3x in TBST and incubated for 1 h with anti-CatL polyclonal IgG (R&D Systems, AF952-SP) diluted 1:1,000 in TBST followed by 30 min with rabbit anti-goat HRP IgG (SouthernBiotech ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cells were removed from adherent cultures using AssayComplete cell dissociation reagent (Eurofins #92-0009) and incubated with a polyclonal anti-ALK2 antibody (R&D Systems #AF637) or a polyclonal anti-ALK3 antibody (Sino Biological #50078-RP02) ...
-
bioRxiv - Neuroscience 2023Quote: ... Trem2 measurements were made using the Mesoscale Discovery (MSD) platform as previously described (29, 105) using a biotinylated anti-mTrem2 polyclonal antibody (R&D Systems BAF1729) as capture antibody and a sulfo-tagged detection antibody (sheep anti-mouse Trem2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... POSTN protein expression in the medium was confirmed using an ELISA assay with an anti-POSTN polyclonal antibody (R&D Systems, Cat # AF3548). 3T3-CTL cells were used to generate “control” conditioned medium (CMCTL) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Sections from formalin-fixed paraffin-wax embedded tumours were stained with primary rat anti-mouse monoclonal anti-CD8 antibody (eBioscience, 4Sm15as) and goat anti-mouse polyclonal anti-PD-L1 antibody (R&D systems, AF1019). Slides were scanned and imaged using Hamamatsu Nanozoomer (Hamamatsu Photonics).
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Then sections were incubated overnight at 4 ◦C with goat polyclonal anti-S100a10 antibody (1:200; AF2377, R&D Systems, Minneapolis, MN, USA), rabbit polyclonal anti-5HT1B Receptor antibody (1:100 ...
-
bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were visualized by incubation with HRP-conjugated IgG Secondary antibody (anti-goat, 1:5000; # HAF017, R&D Systems, goat anti-rabbit ...
-
bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). For Figure 8C WT mouse plasma FABP4 was measured using a commercially available assay for mouse FABP4 (Mouse Adipocyte FABP (FABP4 ...
-
bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). Plasma insulin was assessed using a Crystal Chem Ultra Sensitive Mouse Insulin ELISA ...
-
bioRxiv - Microbiology 2023Quote: ... The following secondary antibody dilutions were made 1:2000 in PBST: goat α-mouse IgG-HRP (R&D Systems; HAF007) and goat α-rabbit IgG-HRP (R&D Systems ...
-
bioRxiv - Biophysics 2021Quote: ... supplemented with 0.3 μg Cholera Toxin Subunit B labeled with Alexa Fluor 555 (V-34404, Vybrant) and 50 nM dimeric EphrinA1-Fc (R&D Systems, #6417-A1). Control experiments were performed without ephrinA1-Fc ...
-
bioRxiv - Immunology 2021Quote: ... Immunostaining nanoparticle tracking analysis (NTA) was performed using fluorescently labeled antibody conjugate directed against human CD63 (AlexaFluor488-conjugated clone 460305; R&D Systems (Minneapolis, USA)) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Incorporation of HNV-RBP and HNV-F were detected with rabbit anti-HA antibody (Novus Cat. No. NB600-363) and rabbit anti-AU1 antibody (R&D systems nnb600-453), respectively ...
-
bioRxiv - Neuroscience 2019Quote: ... anti-α5-GABAA receptor (rabbit, 1:1,000, R&D Systems), anti-NR1 (rabbit monoclonal [1.17.2.6] ...
-
bioRxiv - Cell Biology 2021Quote: ... cleaved Caspase 3 (rabbit, R&D systems MAB835, 1:500), CPE (rabbit ...
-
bioRxiv - Bioengineering 2021Quote: ... horseradish-peroxidase-conjugated rabbit anti-goat (R&D Systems; HAF017), goat anti-rabbit (CST ...
-
bioRxiv - Neuroscience 2022Quote: ... donkey anti-rabbit-A557 (1:2000, R&D systems NL004), goat anti-rabbit-A488 (1:2000 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and rabbit IgG control (1:100 dilution; R&D systems, Minneapolis ...
-
bioRxiv - Neuroscience 2022Quote: ... rabbit anti-activated CASP3 (1:500; R&D Systems, AF835), and rabbit anti-TAK1 (1:500 ...
-
bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) with 0.1% Tween 20) for 30 minutes and probed with primary antibodies: polyclonal goat anti-C3d (R&D systems, AF2655, 1:1000), polyclonal sheep anti-Sez6L2 (R&D Systems ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
-
bioRxiv - Immunology 2023Quote: ... Protein concentration was measured with BCA assay and 350 µg of lysates were incubated with 5 μg of anti-Siglec-E antibody (polyclonal goat IgG - R&D Systems – cat. AF5806) or goat IgG for 2h at 4°C ...
-
bioRxiv - Immunology 2020Quote: ... the membrane was incubated for 1 hr at room temperature (RT) with anti-goat HRP conjugated antibody (R&D Systems #HAF109) at 1:2000 in TBST 1% milk ...
-
bioRxiv - Neuroscience 2022Quote: ... The plates were washed 3 times with washing buffer and a horseradish peroxidase-streptavidin (HRP-Strep) complex (R&D Systems DY998) diluted 1:25 in reagent diluent was added to the plates and incubated for 40 minutes with agitation ...
-
bioRxiv - Neuroscience 2023Quote: The following antibodies were used for in vitro biotinylation (dilutions for each antibody are indicated in parentheses): chicken polyclonal antibody anti-neurofascin (1:1000; R&D Systems Cat# AF3235, RRID:AB_10890736), rabbit polyclonal anti-NrCAM (1:1000 ...
-
bioRxiv - Neuroscience 2023Quote: The following antibodies were used for immunofluorescence studies (dilutions for each antibody are indicated in parentheses): chicken polyclonal anti-neurofascin (1:500; R&D Systems Cat# AF3235, RRID:AB_10890736), chicken polyclonal anti-MAP2 (1:1000 ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were then incubated overnight at 4°C with two primary antibodies: mouse monoclonal anti-HMGB1 (1:1000; HMGBiotech) and goat polyclonal anti-CXCL12 (1:50; R&D Systems, #AF-130-NA). Following three washes with 0.2% BSA/PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... Mouse VEGFR3 domains 1-4 and 4-7 were detected by probing with the polyclonal goat anti-mouse VEGFR3 antibody (R&D Systems, AF743, 1:1000) against the extracellular domain of VEGFR3 ...
-
bioRxiv - Cell Biology 2020Quote: ... Bound NAMPT molecules were detected using a biotinylated antibody for NAMPT and HRP-streptavidin (Human PBEF/Visfatin DuoSet ELISA, R&D Systems). Signals obtained on BSA-coated wells were used to remove non-specific binding for each NAMPT concentrations to obtain specific binding values ...
-
bioRxiv - Developmental Biology 2019Quote: ... A biotin conjugated anti-His-tag antibody (NB100-63172, Bio-Connect) was added followed by a Streptavidin-HRP (DY998, R&D Systems). For a colormetric detection ...
-
bioRxiv - Molecular Biology 2023Quote: ... and bound biotinylated ACE2 and RBD in the presence or absence of SP-A was detected by incubating with Streptavidin-HRP (VANC00B, R&D Systems) for 1 h at room temperature and developed as described above ...
-
bioRxiv - Biochemistry 2023Quote: ... plates incubated with biotinylated antibody were washed and incubated at 37°C for 1 hr with a 1/200 dilution of Streptavidin-HRP (Catalog #890803, R&D systems). Plates were then washed and developed using 100□μl/well SureBlue TMB (3,3′ ...
-
bioRxiv - Molecular Biology 2023Quote: ... the membrane was thoroughly washed with PBS containing 0.05 % Tween-20 (0.05 % PBS-T) and then incubated for 1 h at room temperature with Anti-Mouse IgG HRP-conjugated antibodies (R&D Systems), diluted 1:1000 in the blocking buffer ...
-
bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-Active Caspase-3 (R&D Systems; AF835; 1:250); mouse anti-Mucin 5AC (Abcam ...
-
bioRxiv - Neuroscience 2020Quote: ... phosphorylated PGC-1αS570 (rabbit 1:1000; R&D Systems; RRID: AB_10890391) and β-actin (rabbit 1:1000 ...
-
bioRxiv - Neuroscience 2021Quote: ... Rabbit anti-pMet/HGFR (1:200, Cell Signaling/R&D systems), Rabbit anti-pBTK (1:200 ...
-
bioRxiv - Cell Biology 2022Quote: ... and rabbit anti-CD206/MMR (1:200; AF2535; R&D systems); parasympathetic nerve/ Schwann cell marker ...
-
bioRxiv - Microbiology 2023Quote: ... This was followed by incubation of the cut membranes with primary antibody polyclonal goat anti-human EpCAM (R&D Systems, AF960, 1:100 in SEA BLOCK) overnight at 4°C in a humidified chamber ...