WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin citations for Santa Cruz

An Autoimmune Transcriptional Circuit Driving Foxp3⁺ Regulatory T cell Dysfunction

Tomokazu S. Sumida, et al., bioRxiv - Immunology 2022

Quote: ... the blotted membranes were then incubated overnight with primary antibodies (anti-Blimp1 (C14A4, 1:1,000, Cell Signaling Technologies or C-7, 1:200, Santa Cruz Biotechnology), anti-β-actin (A5441 ...

Integrin β4 promotes DNA damage-related drug resistance in triple-negative breast cancer via TNFAIP2/IQGAP1/RAC1

Huan Fang, et al., bioRxiv - Cancer Biology 2023

Quote: ... cell lysates were first incubated with anti-TNFAIP2/ITGB4/IQGAP1 antibodies or mouse IgG/rabbit IgG (sc-2028;Santa Cruz Biotechnology, CA, USA) and then incubated with Protein A/G plusagarose beads (sc-2003 ...

Styxl2 regulates de novo sarcomere assembly by binding to non-muscle myosin IIs and promoting their degradation

Xianwei Chen, et al., bioRxiv - Developmental Biology 2023

Quote: ... with a cocktail of protease inhibitors and the soluble whole cell lysates were immunoprecipitated with an anti-Myc antibody (Santa Cruz, sc-40). The immunoprecipitated proteins were then analysed by Western blot.

HIV-1 binds dynein directly to hijack microtubule transport machinery

Somayesadat Badieyan, et al., bioRxiv - Microbiology 2023

Quote: ... To determine interaction between BicDR1 and the viral capsid protein p24 cells were permeabilized and blocked using 3% donkey serum followed by incubation with primary antibodies targeting p24 (Santa Cruz sc-69728) and BicDR1 (Atlas Antibodies HPA041309) ...

Model-driven experimental design identifies counter-acting feedback regulation in the osmotic stress response of yeast

Sara Kimiko Suzuki, et al., bioRxiv - Systems Biology 2020

Quote: ... Hog1 was detected using an anti-Hog1 primary-antibody (Santa Cruz, Hog1 antibody (D-3) sc-165978 ...

The chromatin-remodeling enzyme Smarca5 regulates erythrocyte aggregation via Keap1-Nrf2 signaling

Yanyan Ding, et al., bioRxiv - Developmental Biology 2021

Quote: ... The antibodies used were as followings: anti-Smarca5 antibody (Santa Cruz, H-300: sc-13054), anti-β-Actin antibody (Cell Signaling Technology ...

Astrocytes express DMT1 and transferrin receptors, which transport iron thus activating Ca²⁺ signalling: possible role in neuroprotection against iron overload?

Maosheng Xia, et al., bioRxiv - Molecular Biology 2020

Quote: ... Na+/K+-ATPase alpha1/2 antibody and the secondary antibodies were bought from Santa Cruz Biotechnology (Santa Cruz ...

IκBζ is constitutively expressed in human and murine airway epithelium

Kruthika Sundaram, et al., bioRxiv - Immunology 2019

Quote: ... Lamin B1 antibody and beta-actin antibody (monoclonal clone C4) were purchased from Santa Cruz Biotechnology and MP Biomedicals respectively.

Antisense oligonucleotide therapy for SCN2A gain-of-function epilepsy

Melody Li, et al., bioRxiv - Neuroscience 2020

Quote: ... Sections were then incubated with the following antibodies: polyclonal rabbit anti-AnkyrinG antibody (Santa Cruz) at 1:1000 ...

MAF1 is a Chronic Repressor of RNA Polymerase III Transcription in the Mouse

Nicolas Bonhoure, et al., bioRxiv - Molecular Biology 2020

Quote: ... the blot was redetected with an antibody against γ-tubulin (Santa Cruz, antibody B-12).

Actin fence therapy with exogenous V12Rac1 protects against Acute Lung Injury

Galina A. Gusarova, et al., bioRxiv - Physiology 2021

Quote: ... Antibody against GFP (sc-9996) and IκBα antibody (sc-371-G) were from Santa Cruz Biotechnology ...

Mediator of DNA damage checkpoint 1 (MDC1) is a novel estrogen receptor co-regulator in invasive lobular carcinoma of the breast

Evelyn K. Bordeaux, et al., bioRxiv - Cancer Biology 2020

Quote: ... ER IP used antibody sc-543 (Santa Cruz Biotechnology; Santa Cruz, CA; antibody is discontinued) and rabbit IgG ...

Nuclear lamina strain states revealed by intermolecular force biosensor

Brooke E. Danielsson, et al., bioRxiv - Biophysics 2022

Quote: ... The following primary antibodies were used: anti-lamin A antibody (sc-7292, Santa Cruz Biotechnology), anti-mouse LA/C-C (131C3 ...

Cell response to extracellular matrix energy dissipation outweighs rigidity sensing

Carla Huerta-López, et al., bioRxiv - Bioengineering 2022

Quote: ... The following antibodies/reagents were used: YAP antibody (Santa Cruz Biotechnology YAP1 63.7; sc-101199), paxillin antibody (abcam ...

Functional diversity of the TP53 mutome revealed by saturating CRISPR mutagenesis

Julianne Funk, et al., bioRxiv - Cancer Biology 2023

Quote: ... antigens were detected using the antibodies: p53 (Santa Cruz Biotechnology, sc-126; antibody DO-1), p21 (Santa Cruz Biotechnology ...

Evasion of APOBEC1-mediated Intrinsic Immunity by a Herpesvirus Uracil DNA Glycosylase Is a Determinant of Viral Encephalitis

Akihisa Kato, et al., bioRxiv - Microbiology 2023

Quote: Antibodies were purchased as follows: commercial mouse monoclonal antibodies to UL42 (13C9; Santa Cruz Biotechnology), glycoprotein B (gB ...

Establishment of a novel non-integrated human pluripotent stem cell-based gastruloid model

Gege Yuan, et al., bioRxiv - Developmental Biology 2023

Quote: The primary antibodies included: mouse anti-OCT4 antibody (Santa Cruz Biotechnology, Sc-5279; 1:100), rabbit anti-OCT4 antibody (Abcam ...

PAK3 downregulation induces cognitive impairment following cranial irradiation

Haksoo Lee, et al., bioRxiv - Neuroscience 2023

Quote: ... membranes were probed with specific primary antibodies and peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology). The samples were subsequently analyzed using an ECL detection system (Roche Applied Science ...

The proximity-based protein interaction landscape of the transcription factor p65 NF-κB / RELA and its gene-regulatory logics

Lisa Leib, et al., bioRxiv - Molecular Biology 2024

Quote: ... The primary antibody mixture contained PLA and IF antibodies (anti-NF-κB p65 (Santa Cruz Biotechnology ...

Novel fragile X syndrome 2D and 3D brain models based on human isogenic FMRP-KO iPSCs

Carlo Brighi, et al., bioRxiv - Neuroscience 2020

Quote: ... Cells were then incubated overnight at 4°C with primary antibodies at the following dilutions: mouse anti-PAX6 (sc81649 Santa Cruz Biotechnology, 1:50), mouse anti-GFAP (MAB360 Merck Millipore ...

PUMILIO, but not RBMX, binding is required for regulation of genomic stability by noncoding RNA NORAD

Mahmoud M. Elguindy, et al., bioRxiv - Molecular Biology 2019

Quote: ... 5 μg of antibody (anti-PUM1, Santa Cruz sc-135049; anti-PUM2, Santa Cruz sc-31535; anti-RBMX Cell Signaling #14794; Goat IgG control, Santa Cruz sc-2028 ...

Association of NQO1 C609T (Pro187Ser) with risk of Oral Submucous Fibrosis in Eastern Indian population

Sanjit Mukherjee, et al., bioRxiv - Genetics 2021

Quote: ... and incubated with mouse monoclonal NQO1 (1:1000) (Cell Signalling Technology, Beverly, MA) and β-Actin Antibody (C4) (1:2000) (Santa Cruz Biotechnology, USA) as primary antibodies ...

Unconventional stabilization of the human T-cell leukemia virus type 1 immature Gag lattice

Martin Obr, et al., bioRxiv - Molecular Biology 2023

Quote: ... The efficiency of immature particle production was analyzed by quantifying Gag proteins in released particles through harvesting cell culture supernatants and by using immunoblot analysis with a mouse monoclonal anti-HTLV p24 antibody (Catalogue #: sc-53891; Santa Cruz Biotechnology, Dallas, TX). To determine particle production (i.e. ...

Wnt5a-Ror2 signaling activates p62-Nrf2 axis in reactive astrocytes after brain injury

Mitsuharu Endo, et al., bioRxiv - Molecular Biology 2023

Quote: ... on ice, 60 seconds) by a cell disruptor (UD-201, Tomy) and subjected to immunoprecipitation with anti-E2F1 antibody (Santa Cruz Biotechnologies, sc-251) or control mouse IgG (Jackson ImmunoResearch Laboratories ...

Determinants in the HTLV-1 capsid major homology region that are critical for virus particle assembly

Huixin Yang, et al., bioRxiv - Molecular Biology 2024

Quote: The efficiency of immature particle production was analyzed by quantifying Gag proteins in released particles through harvesting cell culture supernatants and by using immunoblot analysis with a mouse monoclonal anti-HTLV p24 antibody (Catalogue #: sc-53891; Santa Cruz Biotechnology, Dallas, TX). Briefly ...

ZNF251 haploinsufficiency confers PARP inhibitors resistance in BRCA1-mutated cancer cells through modulation of RAD51 and DNA-PKcs-dependent pathways

Huan Li, et al., bioRxiv - Cancer Biology 2024

Quote: Nuclear and total cell lysates were obtained as described before(11) and analyzed by SDS-PAGE using primary antibodies against: ATM (Santa Cruz Biotechnology #sc-135663), CtIP (Abcam #ab-70163) ...

Modeling Mechanical Activation of Macrophages During Pulmonary Fibrogenesis for Targeted Anti-Fibrosis Therapy

Ying Xu, et al., bioRxiv - Bioengineering 2023

Quote: ... and Rho associated coiled-coil containing protein kinase 2 (Santa Cruz, sc-398519, 1:100). These antibodies were labeled with fluorophore-conjugated ...

Bitter taste receptor T2R14 and autophagy flux in gingival epithelial cells

Nisha Singh, et al., bioRxiv - Cell Biology 2024

Quote: ... they were grown in medium containing puromycin dihydrochloride (4 μg/ml) (Santa Cruz; sc-108071). The Western blot technique was then used to examine the ATG7 status of both the shRNA transfected cells and the scramble cells in isolated stable clones [23].

TDP-43 deficiency links Amyotrophic Lateral Sclerosis with R-loop homeostasis and R loop-mediated DNA damage

Marta Gianini, et al., bioRxiv - Molecular Biology 2020

Quote: ... were conjugated with 10 μg S9.6 antibody and respective murine IgG antibody (sc-2025, Santa Cruz) for 2h at 4°C in agitation ...

CDC6 regulates both G2/M transition and metaphase-to-anaphase transition during the first meiosis of mouse oocytes

Zi-Yun Yi, et al., bioRxiv - Developmental Biology 2019

Quote: ... rabbit polyclonal anti-bub3 antibody and mouse monoclonal anti-Mad1 antibody were obtained from Santa Cruz Biotechnology (Cat#Sc-28258 ...

Image quantification technique reveals novel lung cancer cytoskeletal phenotype with partial EMT signature

Arkaprabha Basu, et al., bioRxiv - Biophysics 2021

Quote: ... Primary antibodies: Anti-FAK (D1) mouse monoclonal IgG1 antibody (Santa Cruz Biotechnology, catalog no. sc-271126). Secondary antibody ...

Role of FBXW2 in explant culture of bovine periosteum-derived cells

Mari Akiyma, bioRxiv - Cell Biology 2020

Quote: ... Primary antibodies used were mouse monoclonal antibodies for osteocalcin (Santa Cruz Biotechnology, Inc., No. sc-376835), a mouse monoclonal antibody for bovine osteocalcin (code no ...

BTLA and PD-1 employ distinct phosphatases to differentially repress T cell signaling

Xiaozheng Xu, et al., bioRxiv - Cell Biology 2019

Quote: ... SAP monoclonal antibody (#sc-393948) and GRB2 monoclonal antibody (#sc-8034) were obtained from Santa Cruz Biotechnology ...

Exploring therapeutic strategies for Infantile Neuronal Axonal Dystrophy (INAD/PARK14)

Guang Lin, et al., bioRxiv - Neuroscience 2022

Quote: ... Primary antibodies used in this study were as follows: mouse anti-PLA2G6 antibody (Santa Cruz; sc376563), rabbit anti-PLA2G6 antibody (Sigma ...

A DNase T6SS effector requires its MIX domain for secretion

Chaya Mushka Fridman, et al., bioRxiv - Microbiology 2022

Quote: ... The following antibodies were used: α-Myc antibodies (Santa Cruz Biotechnology, 9E10, mouse mAb; sc-40) and custom-made α-VgrG1 (56) ...

The cGAS-STING pathway is dispensable in a mouse model of LMNA-cardiomyopathy despite nuclear envelope rupture

Atsuki En, et al., bioRxiv - Molecular Biology 2023

Quote: ... The primary antibodies were rabbit anti-Lamin A/C antibody (Santa Cruz, sc-20681, 1:1000), rabbit anti-cGAS antibody (Cell Signaling ...

A Yersinia effector activates JAK-STAT signaling in human macrophages

Laura Berneking, et al., bioRxiv - Cell Biology 2023

Quote: ... Primary antibodies used in this study were monoclonal anti-RSK1 (C-21) antibody (Santa Cruz Biotechnology), mouse anti STAT3 antibody Stat3 (Cell signaling) ...

An mRNA processing pathway suppresses metastasis by governing translational control from the nucleus

Albertas Navickas, et al., bioRxiv - Cancer Biology 2021

Quote: ... HNRNPC antibody (Santa Cruz sc-32308) or mouse IgG (Jackson 015-000-003 ...

The cell-cell adhesion protein JAM3 determines nuclear deformability by regulating microtubule organization

Mar Arias-Garcia, et al., bioRxiv - Cell Biology 2020

Quote: ... EB1 Antibody (Santa Cruz, 1:100), YAP (G6 ...

Cell competition acts as a purifying selection to eliminate cells with mitochondrial defects during early mouse development

Ana Lima, et al., bioRxiv - Developmental Biology 2021

Quote: ... HRP-conjugated secondary antibodies (Santa Cruz) were incubated for 1h at RT ...

CD44 inhibits α-SMA gene expression via a novel G-actin/MRTF mediated pathway that requires TGFβR/p38MAPK activity in murine skin fibroblasts

Yan Wang, Judith A. Mack, Edward V. Maytin, bioRxiv - Cell Biology 2019

Quote: ... Primary antibodies purchased from Santa Cruz Biotechnology were anti-CD44 (Cat# sc-7051-R ...