Labshake search
Citations for Santa Cruz :
51 - 100 of 111 citations for Pyruvate Kinase Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... purified exosomes were lysed with a radio-immunoprecipitation assay buffer (Santa Cruz Biotechnology, Dallas, TX, USA), and the cleared lysate was collected by centrifugation for protein separation on 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels ...
-
bioRxiv - Cancer Biology 2021Quote: ... Antibodies used for supershift competition assays were NF-YA (H-209) (Santa Cruz Biotechnology, SC-10779X) and HMGA1a/HMGA1b (Abcam ...
-
bioRxiv - Cancer Biology 2019Quote: ... The per cent reduction was determined by resazurin assay (#sc-206037, Santa Cruz Biotechnology, CA, USA).
-
bioRxiv - Developmental Biology 2024Quote: Cell lysates were prepared by sonication in radioimmunoprecipitation assay lysis buffer (sc- 24948A, Santa Cruz Biotechnology) supplemented with Halt protease and phosphatase inhibitor mixture (78443 ...
-
bioRxiv - Neuroscience 2021Quote: ... The beads were washed four times with 500 μl of radioimmunoprecipitation assay (RIPA) buffer (Santa Cruz Biotechnology) containing the same proteases inhibitors mentioned previously ...
-
bioRxiv - Cell Biology 2022Quote: Assay mixture included 1:2,000 dilution of mouse monoclonal MyBP-C antibody (C0, Santa Cruz, sc-137180), 1:2,000 dilution of rabbit monoclonal MyBP-C antibody (C5-C7 ...
-
bioRxiv - Immunology 2022Quote: Samples were homogenized in Radio Immunoprecipitation Assay buffer containing a cocktail of protease inhibitors (Santa Cruz, CA). Protein extracts were loaded onto 8/15% polyacrylamide gels and then transferred onto a PVDF membrane (Millipore ...
-
bioRxiv - Immunology 2023Quote: Total protein from cells was extracted by lysis in radioimmunoprecipitation assay buffer (Santa Cruz Biotechnology, #sc-24948) with protease inhibitor cocktail (Cell Signaling Technology ...
-
bioRxiv - Developmental Biology 2024Quote: ... Co-IP assay was carried out using protein A/G PLUS-Agarose beads (Santa Cruz, Cat# 2003). Briefly ...
-
bioRxiv - Developmental Biology 2022Quote: Tissue lysates were prepared with radioimmunoprecipitation assay lysis buffer system (sc-24948A, Santa Cruz Biotechnology, Santa Cruz, CA). Protein concentrations were determined using the DC™ Protein Assay Kit (5000112 ...
-
bioRxiv - Developmental Biology 2022Quote: Tissue lysates were prepared with radioimmunoprecipitation assay lysis buffer system (sc-24948A, Santa Cruz Biotechnology, Santa Cruz, CA). Protein concentrations were determined using the DC™ Protein Assay Kit (5000112 ...
-
bioRxiv - Genomics 2023Quote: ChIP assays were performed as described (Strutt and Paro, 1999) using anti-PR (H190 SC-7208, Santa Cruz,); anti-RNApol II (#2629 ...
-
bioRxiv - Biochemistry 2022Quote: Transfected cells were washed with HBSS and lysed in-well with radioimmunoprecipitation assay (RIPA) buffer (Santa Cruz, Dallas, TX) supplemented with Halt Protease Inhibitor (Pierce ...
-
bioRxiv - Cell Biology 2020Quote: Cells were lysed on ice using a radioimmunoprecipitation assay (RIPA) lysis buffer system (Santa Cruz Biotechnology, Dallas, TX, USA) containing PMSF ...
-
bioRxiv - Developmental Biology 2024Quote: ... mouse anti-His (1:500 for immunostaining and 1:1000 for Western blotting assay, Santa Cruz Biotechnology, #sc-53073), rabbit anti-HA (1:1,000 ...
-
bioRxiv - Cancer Biology 2022Quote: ... kit (Santa Cruz Biotechnology, sc-13508), Erk (Cell Signaling Technology ...
-
bioRxiv - Cell Biology 2021Quote: ... mouse anti-Myc (1:1,000 for western blotting and 2 μg for co-IP assays; sc-40; Santa Cruz Biotechnology), and mouse anti-Flag (1:1,000 for western blotting and 2 μg for co-IP assays ...
-
bioRxiv - Molecular Biology 2023Quote: RNase and ATPase assays were performed as described in the presence or absence of guanabenz acetate (sc-203590, Santa Cruz).
-
bioRxiv - Molecular Biology 2023Quote: ... Reactions were quenched by placing the assay tubes on ice and adding 10 μM inhibitor L-685,458 (Santa Cruz Biotechnology). De novo Aβ40 production was quantified by MSD ELISA as described previously48 ...
-
bioRxiv - Neuroscience 2021Quote: ... These total or lysosomal lysates were diluted in 5μl activity assay buffer (see above) or 5μl of 20mM Conduritol B Epoxide (CBE) (Santa Cruz, sc-201356A) (specific GCase inhibitor ...
-
bioRxiv - Plant Biology 2020Quote: ... Detection was carried out by chemiluminescence assays after incubation with an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (Sigma or Santa Cruz).
-
bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used in PLA assays: mouse anti p27 (SX53G8.5, sc-53871), LAMP1 (E-5, Sc-17768), rabbit anti p27 (C19, sc-528) (Santa Cruz Biotechnology), rabbit anti LAMTOR1 (#8975) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was collected and then the protein concentration of each sample was determined by bicinchoninic acid assays (Santa Cruz Biotechnology). Diluted sample and reaction buffer were added into 96-wells plate containing menadione or isoplumbagin with cofactor NADH and water-soluble tetrazolium salt [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] reagent ...
-
bioRxiv - Neuroscience 2022Quote: ... Spinal cord tissue was homogenized by mechanical trituration followed by sonication in radio-immunoprecipitation assay (RIPA) lysis buffer (1 ml; Santa Cruz Biotechnology ...
-
bioRxiv - Cancer Biology 2022Quote: ... we used a kit from Santa Cruz technology ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cell monolayer was washed with cold HBSS three times and lysed by adding 50 μL/well of 1X radioimmuno-precipitation assay (RIPA) cell lysis buffer (Santa Cruz Biotechnology). The plates were maintained at constant agitation at 4°C for 20 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were prepared from culture cells (washed by cold PBS) or tissues by homogenization with a radioimmune precipitation assay lysis buffer (sc-24948, Santa Cruz Biotechnology) containing HaltTM protease and phosphatase inhibitor mixture (catalog no ...
-
Elimination of glutamatergic transmission from Hb9 interneurons does not impact treadmill locomotionbioRxiv - Neuroscience 2021Quote: Tissues were transferred to primary Ab (Anti-DsRed-Rabbit, Rockland antibodies and assays PA, USA 1:2000; Anti-β-gal Goat or Mouse, Santa Cruz Biotechnology ...
-
bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
-
bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
-
bioRxiv - Microbiology 2023Quote: ... Primary antibodies for assay of infected cells were chicken polyclonal anti-UL34 (1:250) (71) and mouse monoclonal anti-LMNA/C (Santa Cruz Biotechnology), both diluted 1:500 ...
-
bioRxiv - Genomics 2023Quote: ... The reaction was incubated on ice for 10 min and super-shift assay was performed by adding 2 µL of anti-HuR antibody (3A2, mouse monoclonal IgG antibody, Santa Cruz Biotechnology). After antibody addition ...
-
bioRxiv - Biochemistry 2022Quote: ... KIT was immunoprecipitated from the supernatant with 6 μg monoclonal anti-KIT antibody (Santa Cruz Biotechnology, no. sc-13508) together with 30 μL protein G PLUS-agarose beads (Santa Cruz Biotechnology ...
-
The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... using a homogeneous protocol of [35S]GTPγS scintillation proximity assay coupled to the use of the following antibodies: mouse monoclonal anti-Gαi1 (1:20, Santa Cruz Biotechnology #sc-13534), rabbit polyclonal anti-Gαi2 (1:20 ...
-
bioRxiv - Neuroscience 2022Quote: ... Spinal cord tissue was homogenized by mechanical trituration followed by sonication in radio-immunoprecipitation assay (RIPA) lysis buffer (1 ml; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and the cytokine concentration were measured using mouse ELISA kits (Invitrogen Life Technologies ...
-
bioRxiv - Cancer Biology 2021Quote: ... The Annexin V apoptosis detection kit (Santa Cruz Biotechnologies) was used to determine the rate of apoptosis ...
-
bioRxiv - Cancer Biology 2023Quote: ... We used the following commercial kits from Santa Cruz technology ...
-
bioRxiv - Neuroscience 2022Quote: ... Brain homogenates were also analyzed through western blotting assays using anti α-synuclein (C-20) primary antibody (sc-7011, from Santa Cruz Biotechnology, Heidelberg, Germany). The bands were quantified by using a densitometric algorithm ...
-
bioRxiv - Cell Biology 2023Quote: ... The anti-c-kit antibody was purchased from Santa Cruz Biotechnology (Santa Cruz ...
-
bioRxiv - Molecular Biology 2019Quote: ... Immunoblots were visualized by enhanced chemiluminescence (ECL kit, Santa Cruz Biotechnology) after washes ...
-
bioRxiv - Molecular Biology 2021Quote: ... and visualized with a chemoluminescence kit (Santa Cruz Biotechnology, sc-2048) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: m6A RNA immunoprecipitation was performed using the Magna RIP Kit (Santa Cruz) according to the manufacturer’s instructions ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... The Chronobiology Kit software program (Stanford Software Systems, Santa Cruz, CA, USA) was used to collect ...
-
bioRxiv - Cancer Biology 2020Quote: ... and CD117 (c-Kit, 2B, 1:200, Santa Cruz Biotechnologies (Santa Cruz, CA)) ...
-
bioRxiv - Cancer Biology 2020Quote: ... and CD117 (c-Kit, 2B, 1:200, Santa Cruz Biotechnologies (Santa Cruz, CA)) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Proteins were detected by immunoblotting with a chemiluminescence Luminol kit (SC-2048, Santa Cruz Biotechnology) using a LumiAnalyst Imager (Roche Applied Sciences).
-
bioRxiv - Biochemistry 2021Quote: ... total RNA was collected from cells using an RNA isolation kit (Zymo, Santa Cruz, CA). Total RNA underwent reverse transcription (RT ...
-
bioRxiv - Immunology 2022Quote: Apoptosis in FLS cultures was measured using a commercial flow cytometry kit (Santa Cruz biotechnology) for Annexin V-FITC binding to phosphatidylserine [28] ...
-
bioRxiv - Biophysics 2023Quote: ... a commercial CRISPR/Cas9-based gene editing kit was employed (Santa Cruz Biotechnology, cat. sc-416469-NIC) including constructs expressing Cas9-D10A nickase ...
-
Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genesbioRxiv - Plant Biology 2022Quote: ... sRNA libraries were constructed using the RealSeq-AC miRNA Library Kit for Illumina sequencing (Somagenics, Santa Cruz, USA) using an input of 150 ng total RNA and 16 PCR amplification cycles ...