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Citations for Santa Cruz :
101 - 150 of 7758 citations for Pig Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Ab-protein-DNA complexes were captured by protein A-G agarose beads (Santa Cruz Biotechnology) for 4 h at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell lysates were pre-cleared with protein A or protein G beads (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2019Quote: EphrinB3 epitopes in myelin extract proteins were neutralized by incubation with anti-EphrinB3 antibodies (AF395, R&D system and sc-271328, Santa Cruz Biotechnology; ratio: 1:1) for 2 h at room temperature prior to the addition to the cells(26).
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bioRxiv - Developmental Biology 2019Quote: ... Embryos of the total protein lysates were used for western blot analysis using anti-Cdk1 antibody (1:300, Santa Cruz) and anti-beta-Actin antibody (1:1000 ...
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Induction of p73, Δ133p53, Δ160p53, pAKT leads to neuroprotection via DNA repair by 5-LOX inhibitionbioRxiv - Neuroscience 2019Quote: ... Membrane was blocked in 5%NFM and then incubated with primary antibodies of following proteins: p53 (1:500, Santa Cruz), Akt p-Akt ...
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bioRxiv - Cancer Biology 2021Quote: ... B-cell lymphoma-extra large (Bcl-xL) and cellular inhibitor of apoptosis protein-1 (cIAP1) were supplied by Santa Cruz Biotechnology (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining lysate was incubated for 2 h with 1.5 µg antibody and subsequently for 1 h with 30 µl protein A/G agarose beads (sc-2003, Santa Cruz), or for 3 h either with 25 µl of M2 anti-FLAG affinity gel (A2220 ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were separated on SDS-PAGE gels and analyzed with western blot (anti-G3BP1, 1:1,000 dilution, Santa Cruz, H-10 ...
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bioRxiv - Biochemistry 2022Quote: ... and the expression of both G3BP1-FRB and FKBP-hADAR(E488Q) proteins was confirmed with western blot (anti-G3BP1, 1:1,000 dilution, Santa Cruz, H-10 ...
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bioRxiv - Molecular Biology 2023Quote: ... as a primary and mouse IgG κ-binding protein horseradish peroxidase (1:50,000 dilution; Santa Cruz Biotechnology, Dallas, United States) as a secondary antibody ...
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bioRxiv - Biochemistry 2024Quote: ... and as secondary antibody 1:5000 diluted mouse IgG kappa binding protein conjugated to Horseradish Peroxidase (Santa Cruz, sc-516102). Nitrocellulose membranes (Cytiva ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysates were pre-cleared with 50μl protein A/G plus agarose beads (Santa Cruz Biotechnology) by rocking at 4°C for an hour ...
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bioRxiv - Physiology 2019Quote: ... 15 μl of 50% (w/v) protein A/G or protein A beads (Santa Cruz Biotechnology) were added to the supernatant and rotated for 30 min to preclear the samples ...
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bioRxiv - Pathology 2019Quote: ... Cell lysates (500μg total protein) were incubated with 20μl Protein A/G PLUS-Agarose (Santa Cruz) by occasional gentle mixing for 6h at 4°C ...
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bioRxiv - Pathology 2019Quote: ... Cell lysates (300μg total protein) were incubated with 20μl Protein A/G PLUS-Agarose (Santa Cruz) by occasional gentle mixing for 12h at 4°C to remove the non-specific binding proteins ...
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bioRxiv - Cell Biology 2023Quote: Protein extraction or immunoprecipitation (IP) using Protein A/G PLUS-Agarose (Santa Cruz biotechnology; sc-2003) (58) ...
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bioRxiv - Cancer Biology 2020Quote: ... CREB binding protein (CBP; Santa Cruz), cyclin A (Novocastra) ...
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bioRxiv - Neuroscience 2020Quote: Protein G PLUS-Agarose (Santa Cruz Biotechnology #sc-2002 ...
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bioRxiv - Physiology 2021Quote: ... Tamm-Horsfall protein (Santa Cruz Biotechnology); Parvalbumin (ThermoScientific ...
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bioRxiv - Biochemistry 2023Quote: ... Protein A/G beads (Santa Cruz) were added to the lysates for 1 hr at 4 °C to capture the precipitate ...
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bioRxiv - Cancer Biology 2021Quote: ... Separated proteins were transferred to nitrocellulose membranes and immunoblotted with primary antibodies specific for PAK3 (Santa Cruz sc-1871, 1:200), c-Jun (CST #9165 ...
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bioRxiv - Cell Biology 2020Quote: ... Between 300 and 500 µg of proteins were incubated with 1 µg of antibody (when using Santa Cruz or Abcam antibodies) or with the concentration recommended by the manufacturer (when using Cell Signaling antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibodies specific for the following proteins were obtained from the indicated suppliers: CD81 (1:1,000, Santa Cruz Biotechnology, Dallas, TX, USA), CD9 (1:1,000 ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed in the Pierce™ IP Lysis Buffer purchased from Thermo Fisher Scientific and 1 mg of protein was allowed to bind overnight in 4°C to agarose-conjugated antibodies (Santa Cruz Biotechnology): ITGB4 (Cat # ...
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bioRxiv - Physiology 2020Quote: ... After the blots had been incubated with a HRP-labeled mouse IgGκ light chain binding protein (1:5000, sc-516102, Santa Cruz), they were reacted with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... One mg of total protein of each sample were immunoprecipitated with 50 ul of p53 antibody (D0-1, sc-126, Santa Cruz) and incubated overnight on rotating wheel at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of protein of cleared lysate were incubated with anti-GFP polysera (1:1,000) overnight prior to addition of protein-G agarose beads (Santa Cruz Biotechnology) for an additional 2 hr ...
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bioRxiv - Immunology 2021Quote: ... Different primary antibodies were used for the detection of interest proteins: anti-IL-1β rabbit polyclonal (1:1000, H-153, SC-7884, Santa Cruz), anti-caspase-1 (p20 ...
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bioRxiv - Microbiology 2022Quote: ... For co-localization studies employing confocal microscopy the following antibody combinations were used: mouse anti-non-structural protein 1 (Santa Cruz)/goat anti-mouse Alexa568 (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... The following primary antibodies were used for detection of epitope-tagged proteins: mouse monoclonal anti-GFP clone B-2 (1:1,000, catalog no. sc-9996, Santa Cruz Biotechnology) and anti-RFP antibody (1/2000 ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µl DynabeadsTM Protein G beads were first coated with 1 µg mouse anti-MYC antibody (sc-40, Santa Cruz Biotechnology) or normal mouse IgG1 (sc-3877 ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting immune complex was then incubated for 1 h with 40 µl of protein A/G plus agarose beads (sc-2003, Santa Cruz). After this ...
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bioRxiv - Neuroscience 2023Quote: ... anti-S6 ribosomal protein (S6) (mouse monoclonal clone C-8, #sc-74459, RRID: AB_1129205, Santa Cruz Biotechnology; 1:1000 for WB), anti-4E-BP1 (rabbit monoclonal clone 53H11 ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody-associated protein complexes were isolated by 2 h incubation with protein A-agarose (Santa Cruz Biotechnology) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant human IgG1-tagged proteins were then purified by the Protein A/G agarose (Santa Cruz Biotechnology). Proteins were eluted with 100 mM glycine pH 3.0 and collected in tubes containing 1 M Tris buffer ...
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bioRxiv - Plant Biology 2021Quote: ... Specific protein levels were assayed by Western blot protein using primary antibodies against GFP (Santa Cruz SC-9996) along with an HRP-conjugated goat anti-mouse IgG secondary antibody (Sigma Aldrich A4416).Total protein was visualized after transfer using Ponceau S red staining ...
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bioRxiv - Immunology 2021Quote: ... The membrane was washed three-times with TBST (TBS containing 0.1% Tween-20) and incubated with HRP-conjugated mouse IgG kappa binding protein (1:5000; Santa Cruz Biotechnology, USA) for 2 hrs at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The membranes were then incubated with HRP-conjugated secondary antibodies (1:5000, anti-Gt, #61-1620, Invitrogen; anti-Rb, #170-5046, Bio-Rad; or mouse IgG kappa binding protein, #516102, Santa Cruz), and the HRP activity was detected by incubation with ECL and exposure to a CCD camera ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated overnight with combinations of the following primary antibodies: rabbit anti-protein kinase C (anti-PKC) clone C20 (1:1000; Santa Cruz Biotechnology), rabbit anti-phospho-histone H3 (1:2500 ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibody membranes were incubated with mouse IgG kappa binding protein conjugated to horseradish peroxidase (1:2500) (sc-516102, Santa Cruz Biotechnology) or goat anti-rabbit IgG conjugated to horseradish peroxidase (1:2500 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were incubated in TBST containing 2% (w/v) BSA with mouse IgG kappa binding protein conjugated to horseradish peroxidase (1:5000) (sc-516102, Santa Cruz Biotechnology) or goat anti-rabbit IgG conjugated to horseradish peroxidase (1:5000 ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used for Western blot of β-actin and AK2 proteins were: anti-actin (Santa Cruz Biotechnology sc-47778, 1:1000), anti-AK2 (ProteinTech 11014-1-AP ...
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bioRxiv - Bioengineering 2020Quote: Tissue sections were incubated overnight with polyclonal rabbit antibodies raised against c-fos protein (1:1000; Santa Cruz Biotechnology Inc.; sc-253), GFAP (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatants were pre-cleared with control IgG for 1 hr at 4 °C and Protein A/G Plus-Agarose beads (Santa Cruz Biotechnology) for 30 min at 4 °C before incubation with primary antibody overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Immunodetection of ubiquitinated proteins was accomplished by incubating the membrane overnight with 1:1000 diluted monoclonal ubiquitin antibody P4D1 (sc-8017; Santa Cruz Biotechnology) and exposing the membranes to peroxidase-conjugated anti-rabbit secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed and incubated with mouse IgG kappa-binding protein conjugated to horseradish peroxidase (m-IgGk-HRP, 1:4000) (Santa Cruz technologies) in 5% milk for 1 h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mg of total protein cell lysate was incubated with 4 µg of rabbit anti-human-ERG Ab (Santa Cruz: #sc-28680) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... approximately 750 µg of solubilized proteins were incubated with 1 µg of anti-C/EBPβ mouse IgG (Santa Cruz Biotech, sc-7962) or normal mouse IgG (Santa Cruz Biotech ...
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with mouse IgG kappa binding protein (m-IgGκ BP) conjugated to HRP (1:2000) (sc-516102, Santa Cruz Biotechnology) for 3h at 4°C on a rotary and washed thrice with TBST for 10 min each time ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μl Dynabeads protein G (Santa Cruz) were added to the lysate for 1 h at 4°C ...