Labshake search
Citations for Santa Cruz :
1 - 50 of 51 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... using high-binding 96-well plates (Santa Cruz Biotechnology). After incubation at 4°C overnight ...
-
bioRxiv - Biochemistry 2019Quote: ... 50 ul of the conditioned medium from each transfection was incubated in one well of a 96-well ELISA plate (UltraCruz® ELISA Plate, high binding, 96 well, Flat bottom, Santa Cruz Biotechnology) at 37°C for 1 hr to allow protein in the media to adsorb to the plates ...
-
bioRxiv - Immunology 2020Quote: ... polystyrene 96-well high-bind plates (Santa Cruz Biotechnology, USA) were coated with 100 µl of 1 µg/mL of S1 ...
-
bioRxiv - Bioengineering 2019Quote: ... MART1and PFK transcripts were analyzed by SYBR Green–based real-time quantitative RT-PCR (qRT-PCR) using specific primers purchased from Santa Cruz. Data were normalized to the expression of RPL19 and are expressed as fold changes.
-
bioRxiv - Immunology 2022Quote: ... Plates were developed using TMB substrate buffer (sc-286967, Santa Cruz), and read with the Tecan reader.
-
bioRxiv - Immunology 2021Quote: ... For EMCV plates were fixed with 4% paraformaldehyde (Santa Cruz Biotechnology) at 24h post-infection followed by staining with a 5% crystal violet solution prepared by dissolving crystal violet (Sigma Aldrich ...
-
bioRxiv - Immunology 2021Quote: ... For IAV plates were fixed with 4% paraformaldehyde (Santa Cruz Biotechnology) at 24h post-infection followed by staining with a 5% crystal violet solution ...
-
bioRxiv - Immunology 2021Quote: ... For CVB plates were fixed with 4% paraformaldehyde (Santa Cruz Biotechnology) at 24h post-infection followed by staining with a 5% crystal violet solution ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... the plates were pre-coated with antibody against AP2M (1:500, #516102, Santa Cruz) in carbonate buffer ...
-
bioRxiv - Microbiology 2023Quote: ... Twenty-four well polystyrene plates (UltraCruz, Santa Cruz Biotechnology, USA, Cat No. sc-204445) were seeded with 1.75×105 Vero cells/well in 500 µL of CGM ...
-
bioRxiv - Microbiology 2023Quote: ... onto 24-well polystyrene plates (UltraCruz, Santa Cruz Biotechnology, USA, Cat No. sc-204445) and incubated at 37°C in 5% CO2 for 24 hours ...
-
bioRxiv - Cancer Biology 2021Quote: ... 96-well plates were coated with fibronectin (10 µg/ml; sc-29011; Santa Cruz Biotechnology) at 4°C overnight ...
-
bioRxiv - Cancer Biology 2021Quote: ... 96-well plates were coated with fibronectin (10 μg/ml; sc-29011; Santa Cruz Biotechnology) at 4°C overnight ...
-
bioRxiv - Pathology 2019Quote: siRNA transfection of QSG-7701 cells in 6-well plate with Sp1siRNA (Santa Cruz, sc-29487) was carried out using Lipofectamine® RNAiMAX Reagent ...
-
bioRxiv - Molecular Biology 2019Quote: ... 5 μl of renatured proteins were mixed in a white 96-well plate (Santa Cruz Biotechnology) with ATP (100 uM final concentration ...
-
bioRxiv - Plant Biology 2020Quote: ... the seedlings were transferred into MS plates containing the following SL inhibitors: 0.1 µM myriocin (Santa Cruz), 5 µM FB1 (Santa Cruz) ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plates were then incubated with HRP-conjugated mouse IgG kappa binding protein (1:5000, #516102, Santa Cruz) for 2 h at RT ...
-
bioRxiv - Bioengineering 2023Quote: ... Plates were washed twice with sterile PBS before adding 0.05mg/mL Collagen type-I (Santa Cruz: SC-136157). Plates were covered and left in the 4°C overnight ...
-
bioRxiv - Synthetic Biology 2019Quote: Maxisorp plates (ThermoScientific) were incubated with 100 μL/well of mouse monoclonal Stx2 antibody (Santa Cruz Biotechnology, sc-52727) diluted 1:2500 into PBS for 1.5 h at r.t. ...
-
bioRxiv - Microbiology 2021Quote: ... 1 × 105 HepG2 cells in 24-well plates were pretreated with 8 μg/mL polybrene (Santa Cruz, CA, USA) for 2 h ...
-
bioRxiv - Physiology 2022Quote: ... podocytes growing in a 6-well plate were transfected with 0.75µg (or 60 pmols) msDynll1 siRNA Oligo Duplex (Santa Cruz #sc-36229 ...
-
bioRxiv - Plant Biology 2023Quote: ... Auxin synthesis inhibitor treatments were done by growing gemmae on plates made with 10µM 5-(4-chlorophenyl)-4H-1,2,4-triazole-3-thiol (yucasin) (ChemCruz, Santa Cruz Biotechnology) or 100µM L-kynurenine (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... and stored in 96-well flat-bottom plates filled with 0.02% sodium azide PBS at 4 °C (Santa Cruz Biotechnology).
-
bioRxiv - Developmental Biology 2021Quote: Cultured cells from the indicated time points were washed with PBS and fixed in 24 well plates using 4% Paraformaldehyde Solution (Santa Cruz) for 20 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were plated in 48-well plates (2×105 cells/well in 600μL) and mixed with polybrene (Santa Cruz Biotechnology, 134220) to a final concentration of 4μg/mL and 100-200μL undiluted lentiviral supernatant ...
-
Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: Monolayers of CHO cells grown on multi-well plates were washed 3 times with ice-cold PBS and incubated in RIPA buffer (24948A, Santa Cruz) with protease inhibitors (2498 ...
-
bioRxiv - Immunology 2020Quote: ... were plated in 48-well culture plates for 24 h prior to transfection with human TLR2 siRNA (sc-4025, Santa Cruz) or non-targeting control siRNA (sc-37007 ...
-
bioRxiv - Immunology 2021Quote: ... Invitrogen Nunc MaxiSorp 96-well plates were coated with 50 μl of capture antibody diluted 1:5000 (Santa Cruz, sc-57201) in 0.1 M carbonate buffer and were incubated at 4°C overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded in a 12-well plate at 2x106 cells per well in 1 mL standard media supplemented with 10 μg/mL polybrene (Santa Cruz). Lentivirus was placed in the wells ...
-
bioRxiv - Microbiology 2020Quote: ... the cells were grown in 6-well tissue culture plates and transfected with 80 pmol EGFR siRNA (sc-29301, Santa Cruz Biotechnology), and EphA2 siRNA (sc-29304 ...
-
bioRxiv - Cell Biology 2019Quote: ... BMSCs (0.5 × 106) were seeded on a 6-well culture plate and transfected with Cav1.2 siRNA or Cav1.3 siRNA (Santa Cruz, Dallas, Texas, USA) using X-tremeGENE siRNA Transfection Reagent (Roche ...
-
bioRxiv - Synthetic Biology 2019Quote: ... Dox-treated Gal4-EED/luc cells were transfected in 12-well plates and then grown for 24 hours before the addition of 10 µg/mL puromycin (Santa Cruz Biotechnology) to Gibco DMEM high glucose 1× (Life Technologies ...
-
bioRxiv - Immunology 2020Quote: ... Cells were then plated in 96-well U-bottom plates at 1×106 per 200 μl in the presence of 30 μM Raltegravir (Santa Cruz Biotechnology) and the indicated activator ...
-
bioRxiv - Immunology 2022Quote: 96-well Enzyme ImmunoAssay/Radio ImmunoAssay (EIA/RIA) plates (#3590, Costar) were coated overnight at 4°C with an anti-elastase antibody (1:250, #sc-9521, Santa Cruz Biotechnology) in 15 mM of Na2CO3 ...
-
bioRxiv - Microbiology 2022Quote: Approximately 1 × 105 CHO-HVEM cells were plated in a 24-well plate and the subsequent day transfected with siCtrl (Santa Cruz Biotechnology) or siNEDD8 (Santa Cruz Biotechnology ...
-
bioRxiv - Microbiology 2023Quote: ... OKF6/TERT2 cells were grown in 6 well plates to 80% confluency and transfected with 40 pmole of c-Met siRNA (#SC-29397, Santa Cruz Biotechnology) or E-cadherin siRNA (#SC-35242 ...
-
bioRxiv - Neuroscience 2023Quote: ... The remaining cell suspension was then incubated in a positive-selection plate coated with anti-PDGFRα antibodies (catalog no. sc-338; Santa Cruz Biotechnology). The attached cells were collected using trypsin and cultured on poly(L-lysine)-coated coverslips in proliferation medium containing Dulbecco’s modified Eagle’s medium (DMEM ...
-
bioRxiv - Biochemistry 2023Quote: ... 80% confluent RHMVECs in 6-well plates were transduced with human T111I EL (pKS18) lentiviruses with 4 μg/ml Polybrene (#134220; Santa Cruz Biotechnology) in a total volume of 1 ml DMEM ...
-
bioRxiv - Genomics 2022Quote: Parental U2OS and RPE-1 cells were seeded in 6-well plates and the next day transfected with RFX7 CRISPR/Cas9 KO Plasmid (#sc-408041, Santa Cruz Biotechnology) using Lipofectamine 3000 (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2020Quote: Ishikawa cells were grown in a 6-well plate in full media and co-transfected with the PEA3 (alias for ETV4) CRISPR/Cas9 KO plasmid (Santa Cruz, sc-422185) and the PEA3 HDR plasmid (Santa Cruz ...
-
bioRxiv - Cell Biology 2020Quote: ... all media was removed from the plate and replaced with 8 ml of 2 µg/ml puromycin (Santa Cruz Biotech cat # sc-108071) L-15 selection media ...
-
Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Neutralizing antibodies were quantified like the ELISA method described above in a 384-well UltraCruz® ELISA high-binding plate (Santa Cruz Biotechnology). Diluted spike in 0.05 M carbonate-bicarbonate buffer (1 μg spike/mL ...
-
bioRxiv - Immunology 2020Quote: ... Plates were then washed 4 times and incubated with anti-IgG1 mouse-horseradish peroxidase (HRP) conjugate (1:2000 dilution; Santa Cruz Biotechnology Inc.) at RT for 1 h ...
-
bioRxiv - Microbiology 2021Quote: ... Putative single-allele gyrA (gx) and parC (py) mutants were selected on TSYA plates supplemented with 0.5 mg/L sparfloxacin (Santa Cruz Biotechnology, Texas, USA) or 4 mg/L ciprofloxacin (Acros Organics ...
-
bioRxiv - Cell Biology 2022Quote: ... plates were flooded with detection solution containing 0.1 mg mL−1 2-naphthyl sulfate (potassium salt; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) as a chromogenic substrate coupled with 1 mg mL−1 tetrazotized-o-dianisidine chloride (Fast Blue B salt ...
-
bioRxiv - Molecular Biology 2024Quote: ... U2OS cells were seeded in 6-well plates and virus-containing supernatant was added to cells together with Polybrene (10 mg/mL) (Santa Cruz Biotechnology Inc). Cells were then spin-infected for 100 minutes at 2000 rpm at 37 °C ...
-
bioRxiv - Microbiology 2022Quote: ... were seeded in 24-well plates and treated for 24 hours with 30 pmol siRNA from either Nox1 siRNA (sc-43939; Santa Cruz Biotechnology, Inc, U.S.A) or Ambion® Silencer Negative Control #1 siRNA (Invitrogen ...
-
bioRxiv - Cancer Biology 2019Quote: A total of 3-4 x 105 microglia were plated in a 6-well plate and treated with 10 μg/ml immunoglobulin IgG1 control antibody (Santa Cruz Biotechnology, sc-2025) or JAM-A-blocking antibody J10.4 (Santa Cruz Biotechnology ...
-
bioRxiv - Immunology 2022Quote: ... Half-volume ELISA plates (Fisher, #07-200-37) were coated overnight with 100 µL rat-α-mouse IFN-β antibody (Santa Cruz, # sc-57201) at 0.1 µg/mL in plate coating buffer (0.1 M carbonate [Thermo ...
-
bioRxiv - Cell Biology 2022Quote: ... BJAB ERAAP-dsRed cells were seeded at 1X10^6 cells/well in a 12-well plate and spin-infected with Cas9 expressing lentivirus in presence of 4ug/mL polybrene (Santa Cruz Biotechnology, sc-134220) by centrifugation at 1,000 g for 2 h at 33 °C ...