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Citations for Santa Cruz :
151 - 200 of 236 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... These total or lysosomal lysates were diluted in 5μl activity assay buffer (see above) or 5μl of 20mM Conduritol B Epoxide (CBE) (Santa Cruz, sc-201356A) (specific GCase inhibitor ...
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bioRxiv - Immunology 2021Quote: ... total protein was extracted by lysing tissue in ice-cold RIPA buffer supplemented with protease inhibitor cocktail (Santa Cruz #sc-24948), sodium orthovanadate ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: Monolayers of CHO cells grown on multi-well plates were washed 3 times with ice-cold PBS and incubated in RIPA buffer (24948A, Santa Cruz) with protease inhibitors (2498 ...
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bioRxiv - Neuroscience 2020Quote: ... boiled in 50 µl 2X sample buffer and analysed by Western blotting using a mouse anti-GFP primary antibody (Santa Cruz) and HRP-conjugated goat anti-mouse IgG secondary antibody (Thermo-Fisher).
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bioRxiv - Neuroscience 2019Quote: ... for 45 minutes and incubated with primary anti RAGE antibody diluted in blocking buffer (0.2% BSA in PBS containing 0.02% Tween20, Santa Cruz Biotechnology, USA) for overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Total protein from cultured cells was homogenized in RIPA lysis buffer with a mixture of protease and phosphatase inhibitors (Santa Cruz). Protein concentration was determined using the Bradford assay (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked with clear milk blocking buffer (Fisher, cat #PI37587) for 1 hour at room temperature and incubated with primary antibodies against LPGDS (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2021Quote: ... One mg of cell lysate in 500 μL lysis buffer was pre-cleared by adding 10 μL of protein A/G PLUS-agarose beads (Santa Cruz) and incubating for 1 hour at 4°C on a rotator ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies diluted in 1% NGS blocking buffer were added to the cultured cells overnight at 4°C (Oct4, 1:100, Santa Cruz; β-III-tubulin 1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... After a further centrifuge red blood cells were lysed in 1 ml of RBC lysis buffer (Santa Cruz Biotechnology sc-296258) in ice for 2.5 minutes ...
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bioRxiv - Neuroscience 2021Quote: Protein samples were prepared from microdissection of 10 hippocampi of juvenile mice incubated in RIPA lysis buffer (Santa Cruz sc-24948). Active Rac1 Detection was performed using Active Rac1 Detection Kit of Cell Signalling Technology ...
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bioRxiv - Systems Biology 2022Quote: ... overnight at 4°C diluted 1:500 in blocking buffer and incubated with secondary antibodies (anti-mouse HRP, Sc516102-CM, Santa Cruz) diluted 1:5000 in blocking buffer for one hour at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Spinal cord tissue was homogenized by mechanical trituration followed by sonication in radio-immunoprecipitation assay (RIPA) lysis buffer (1 ml; Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2023Quote: Cell monolayers were rinsed twice with PBS and lysed on ice for 10 min with cold RIPA lysis buffer (1× TBS, 1% Nonidet P-40, 0.5% sodium deoxycholate, and 0.1% SDS) containing protease inhibitors (Santa Cruz, Dallas, TX) and a phosphatase inhibitor cocktail (Pierce/ThermoFisher ...
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Broad variation in response of individual introns to splicing inhibitors in a humanized yeast strainbioRxiv - Molecular Biology 2023Quote: ... the membrane was probed with anti-GFP antibodies in blocking buffer (primary anti-GFP mouse monoclonal, Santa Cruz Biotechnology 1:200), visualized using goat anti-mouse IRDye 680 secondary antibody in blocking buffer at 1:15,000 (Li-Cor) ...
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bioRxiv - Immunology 2023Quote: ... washed with PBS and stained in blocking buffer overnight at 4°C with anti-CDC42 (B-8 mouse monoclonal, Santa Cruz), biotin-conjugated anti-CD44 (IM7 rat monoclonal ...
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bioRxiv - Biophysics 2024Quote: ... The cell pellets were washed with PBS (Quality Biological) and then resuspended in 100ul of RIPA lysis buffer (Santa Cruz Biotechnology) containing protease inhibitors ...
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bioRxiv - Cell Biology 2024Quote: TMT labelled pooled peptides were resuspended in phosphatase buffer (50 mM HEPES, 100 mM NaCl, 1 mM MnCl2 (pH 7.5) and Lambda phosphatase (Santa Cruz Biotechnologies) was added according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were lysed in ice-cold RIPA lysis buffer containing protease inhibitors (sc-24948, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and centrifuged at 12,000 rpm for 20 min at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were lysed in ice-cold RIPA lysis buffer containing protease inhibitors (sc-24948, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and centrifuged at 12,000 rpm for 20 min at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitations were washed with low and high salt wash buffers and RNA was eluted from the beads with 5 mM m6A salt (Santa Cruz Biotechnology).
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bioRxiv - Molecular Biology 2019Quote: ... total RNA in 500 μl of water was incubated at 65°C for 10 minutes and incubated with 3 μl of oligo dT-Biotin (dT-B; IDT Technologies) (50 pmol/μl) and 13 μl of SSC Buffer 20X (Santa Cruz Biotechnology) and allowed to cool at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cell monolayer was washed with cold HBSS three times and lysed by adding 50 μL/well of 1X radioimmuno-precipitation assay (RIPA) cell lysis buffer (Santa Cruz Biotechnology). The plates were maintained at constant agitation at 4°C for 20 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Bound material was eluted and processed in 1X Laemmli buffer for Western blotting and detected with anti-GFP antibody (1:6000, sc-9996, Santa Cruz Biotechnology).
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bioRxiv - Cell Biology 2021Quote: ... and isolated Golgi after lysis in the RIPA Lysis Buffer System with protease and phosphatase inhibitors (sc-24948, Santa Cruz Biotechnology, USA). Protein levels were quantified using the Pierce™ BCA Protein Assay Kit (23225 ...
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bioRxiv - Bioengineering 2021Quote: ... for 1 hour at room temperature and incubated with primary antibody in blocking buffer (1:100 dilution of goat anti-CEP164, sc-240226 from Santa Cruz biotechnology) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: Prefixed (4% PFA) MOs sections (70um) were treated with 400 Units of lambda phosphatase reaction buffer (Santa Cruz, cat no. sc-200312A) for 30 minutes at 37C ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared from culture cells (washed by cold PBS) or tissues by homogenization with a radioimmune precipitation assay lysis buffer (sc-24948, Santa Cruz Biotechnology) containing HaltTM protease and phosphatase inhibitor mixture (catalog no ...
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bioRxiv - Plant Biology 2021Quote: ... the protein-retained beads were boiled in Laemmli buffer and immunoblotted using anti-GST and anti-MBP antibodies (Santa Cruz Biotechnology, USA).
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bioRxiv - Cell Biology 2019Quote: ... the membrane was blocked with 5% fat-free milk in PBS buffer for 1h and then incubated for 3h with a rabbit primary antibody anti-CC10 1:500 (Santa Cruz Biotechnology) in blocking buffer at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... 0.25×106 cells were resuspended in 200μl of staining buffer containing anti-CD98 antibody (CD98-Alexa680, Santa Cruz Biotechnology, Inc., sc-59145) or respective isotype control (mouse IgG-Alexa680 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein precipitates were then re-suspended in 50 µL of Laemmli loading buffer and subjected to 10% SDS-PAGE separation and detection of CDK9 (C-20, sc-484, Santa Cruz Biotechnology).
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bioRxiv - Cell Biology 2021Quote: The cell monolayers were rinsed with Hank’s balanced salt solution (HBSS) and lysed with ice-cold RIPA buffer (Santa Cruz, Dallas, TX) containing protease (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were blocked with 3% non-fat milk-0.05% Tween-20 in Tris buffer saline (TTBS) and incubated with primary antibody anti-GFP (rabbit polyclonal, 1:1000 Santa Cruz Biotechnologies). After wash-out ...
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bioRxiv - Immunology 2020Quote: ... m6 A-modified RNA was eluted twice in 100 mL of MeRIP buffer containing 5 mM m6 A salt (Santa Cruz Biotechnology) for 30 min at 4C with rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibody mouse IgGκ-HRP was diluted at 1:5000 in block buffer and incubated at 1 hour at room temperature with rocking (Santa Cruz Biotechnology). Proteins were visualized with Supersignal West Pico PLUS chemiluminescent substrate (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with 20 ng/ml IL-6 and 4 hours post-treatment lysed using CHAPS buffer and incubated overnight with 2 μg of an anti-ETV7 antibody (TEL2, Santa Cruz Biotechnologies) or normal mouse IgG (Santa Cruz Biotechnologies ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were all used at 1:1,000 in blocking buffer (SM2, Abclonal #A4064; SMA Abclonal #A17910; EGFP, Santa Cruz #SC-9996)
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated overnight at 4°C in the following primary antibodies diluted in a solution of blocking buffer with 0.2% Tween-20: mouse anti-GapDH (1:1000, Santa Cruz #sc-365062), rabbit anti-ERβ (1:250 ...
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bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
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bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were incubated overnight with RIP immunoprecipitation buffer containing magnetic Protein G beads conjugated with human SFPQ/PSF antibody (sc-271796, Santa Cruz, Cliniscience) or negative control mouse IgG (5415S ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were blocked in 1xPBS + 0.1%Tween (1xPBST) buffer with 6% Blotto non-fat milk powder (Santa Cruz Biotechnologies, Dallas, TX). Following 4 washes in 1xPBST shaking for 5 minutes each ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were stopped with SDS-PAGE sample buffer and analyzed by Western blotting with anti-Gγ2 (7-RE20, Santa Cruz sc-134344) and anti- Gβ1 (Sigma-Aldrich sab2701168).
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bioRxiv - Immunology 2023Quote: ... 1 mg of hTCEpi cell lysate in cell lysis buffer was precleared with 25 μl of protein A/G plus agarose (SC-2003; Santa Cruz Biotechnology) for 2 hours at 4°C with rotation before the agarose was removed by centrifugation at 2,000 rcf for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... by washing 3 times with phosphate-buffer saline (PBS) followed by incubation with either 10 µg of mouse monoclonal anti-Ndc80/Hec1 antibody (Santa Cruz, sc-515550) or 10 µg of mouse IgG (Sigma-Aldrich ...
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bioRxiv - Physiology 2019Quote: ... slides were incubated overnight at 4°C with the following antibodies (diluted in blocking buffer): Anti PECAM-1 (sc-376764, Santa Cruz, 1:50), anti Ki67 (AB9260 ...
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bioRxiv - Cancer Biology 2021Quote: ... The protocol for the Bond Rx platform included ER2 (High pH buffer) −30 minutes for Heat retrieval followed by 30 minutes incubation time for Primary Abs (Santa Cruz Biotech, US). Polymer Detection was through DAB Kit (catalog DS9800) ...
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bioRxiv - Bioengineering 2021Quote: ... the membrane was washed three times with Tris buffer and incubated for one hour with anti-mouse antibodies conjugated with horseradish peroxidase (Santa Cruz Biotechnology, USA). After washing the membrane ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with one of the following anti-human primary antibodies diluted in blocking buffer: goat PAX3 (1:100; 4°C overnight; Santa Cruz, sc-34916), mouse TYRP1 (MEL-5 ...