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Citations for Santa Cruz :
601 - 634 of 634 citations for IL 10 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... protein lysates (500 μL of NP-40 buffer containing 200 μg of protein) were incubated with 10 μg of primary antibody Neu (F-11) agarose conjugate (sc-7301; Santa Cruz Biotechnology) overnight at 4°C with gentle rotation ...
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bioRxiv - Biochemistry 2022Quote: ... The elution was separated by 10% Nu-PAGE and analyzed by CBB staining or immunoblotting using an antiubiquitin antibody (Santa Cruz Bio.).
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed three times for 10 min with TBS/T and incubated for 1 h with goat anti-mouse IgG-HRP (sc-2005 Santa Cruz Biotechnology) or mouse anti-rabbit IgG-HRP (sc-2357 Santa Cruz Biotechnology ...
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bioRxiv - Biophysics 2023Quote: PRE measurements were made using 15N-labeled Cys1253Ala and Cys1253Ala/Thr1273Glu mutants of the S tail peptide that were reacted with 10 molar equivalents of MTSL (Santa Cruz Biotechnology) for 1-2 hours at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... cells were given fresh medium containing 5 mM [13C5]-4-HV (synthesized by WuXi) or 10 mM unlabeled 4-HV sodium salt (Santa Cruz Biotechnology). Cells were cultured with 4-HV for 6 hours prior to harvest ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies were added overnight at 4C in 5% milk powder or 10% roti block (Roth) – 1:1000 Ub antibody P4D4 (Santa Cruz Biotechnology), 1:3000 Hip1 (Proteintech 22231-1AP) ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was separated by centrifugation at 12.000 g at 4 °C for 10 min and incubated with STAT2 antibody (2 µg) (Santa Cruz Biotechnology, 514193) for 3 h at 4°C with gentle shaking ...
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bioRxiv - Cancer Biology 2024Quote: ... the membranes were incubated overnight at 4°C with the following primary antibodies: mouse anti-PPM1D (F-10, Santa Cruz, 1:1000), mouse anti-GAPDH (MAB374 ...
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bioRxiv - Cell Biology 2020Quote: ... by washing 3 times with phosphate-buffer saline (PBS) followed by incubation with either 10 µg of mouse monoclonal anti-Ndc80/Hec1 antibody (Santa Cruz, sc-515550) or 10 µg of mouse IgG (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... were washed with 200 μL HBS (150 mM NaCl, 10 mM HEPES, pH7.5 by KOH) and incubated with 2 μg anti-HA (Santa Cruz, Cat.# sc-7392) or 2 μg rabbit IgG isotype (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... permeabilized in PBS with 10% triton-x for 30 min and incubated for three days at 4 °C with goat anti-DCX antibody (1:250 in 10% triton-x and 3% horse serum (sc-8066; Santa Cruz Biotechnology, USA). Sections were washed and incubated in biotinylated donkey anti-goat secondary antibody for 60 minutes (1:250 ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were incubated at 37 °C in 5% O2 and 5% CO2 in EN media with 10 μM ROCK inhibitor (Y-27632, Santa Cruz, sc-281642A). Organoids were grown as 40 μl Matrigel domes submerged under identical medium at initial seeding density of KRT5+ 20,000 cells/dome ...
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bioRxiv - Cancer Biology 2020Quote: ... n=10) and were treated with 5 mg/kg isotype specific IgG (IgG1; West Lebanon, New Hampshire) or mAb (Santa Cruz, Dallas, TX) 3 times per week ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-CECR2 (C3, sc-514878), mouse anti-CSF1 (D4, sc-365779), mouse anti-NF-κB p50 (E-10, sc-8414) (Santa Cruz, Dallas, TX); rabbit anti-NF-κB p65 (D14E12 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then blocked in 10% normal goat serum (NGS) in PBS for 1h at room temperature followed by staining with: mouse anti-ERα (F-10) monoclonal antibody (Santa Cruz, #sc-8002) 1:50 ...
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bioRxiv - Genomics 2023Quote: ... blocked with 10% NGS and incubated with primary antibody against AKT (cat#9272, 1:200 dilution) and COL1A2 (cat#sc-393573. Santa Cruz. 1:200) followed by appropriate fluorescence conjugated secondary antibodies (Alexa 568-tagged for AKT ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were added to the lysate at a final concentration of 10 ug/mL: mouse anti-Anks1b (Santa Cruz #sc-376610), mouse anti-Scn2a (Antibodiesinc #75-024) ...
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bioRxiv - Developmental Biology 2024Quote: ... Supernatant was transferred to a new micro-tube and precleared using 10 μl of protein A/G bead (Santa Cruz Biotechnology, sc-2003). It was then incubated with 0.5 μg of anti-Myc antibody for 1 hr at RT and incubated 20 μl of protein A bead for O/N at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... U2OS cells were seeded in 6-well plates and virus-containing supernatant was added to cells together with Polybrene (10 mg/mL) (Santa Cruz Biotechnology Inc). Cells were then spin-infected for 100 minutes at 2000 rpm at 37 °C ...
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bioRxiv - Bioengineering 2019Quote: ... Viral titer was determined by serial dilution and infection of HUVECs in the presence of 10 µg ml−1 polybrene (Santa Cruz Biotechnology, Dallas, TX). Titers yielding maximal expression without cell death or detectable impact on cell proliferation or morphology were selected for studies.
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bioRxiv - Cancer Biology 2020Quote: ... The filtered viral supernatant was used immediately to transduce target cells (PDACc/PANC-1 or fibroblasts) with 10 µg/mL polybrene (Santa Cruz Biotechnology, Dallas, Texas) or stored at −80°C for use in the future ...
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bioRxiv - Neuroscience 2020Quote: ... washed 3 × 10 min with TBST and incubated with the secondary antibody HRP-conjugated goat anti-mouse (1:10000, Santa Cruz Biotechnology #sc-2005) in 5% BLOTTO for 1 hr at RT and washed again 3 × 10 min with TBST ...
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bioRxiv - Systems Biology 2021Quote: ... lentivirus was recovered from supernatant using an Amicon Ultra Centrifugal 50kDa filter (MilliporeSigma) and transduced into HeLa cells along with 10 μg/ml polybrene (Santa Cruz Biotechnology, Dallas, TX). Cells were transduced sequentially with PKAR-FKBP-FP followed by Lyn-FRB ...
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bioRxiv - Cell Biology 2020Quote: ... and β5-integrin cells were incubated for 20 min before chase and during pulse with 10 μM gefitinib (Santa Cruz Biotechnology, 184475-35-2), 10 μM PP2 (Thermo-Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The excess ECL reagent was removed using a kimwipe and the membrane was placed in between plastic sheet in a cassette holder and developed on an 8” X 10” UltraCruz autoradiography film (Santa Cruz Biotechnology; #SC-201697) in a developer.
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bioRxiv - Molecular Biology 2022Quote: Proteins were separated by SDS-PAGE (7.5% or 10% gels) followed by immunoblot analysis on Immobilon-P PVDF membranes (MilliporeSigma; IPVH00005) using mouse monoclonal α-Myc antibody (9E10; Santa Cruz; sc-40), α-HA (16B12 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 × 106 Ba/F3 were re-suspended in the viral media and supplemented with 10 μg/mL polybrene (Santa Cruz Biotechnology, Dallas, TX, USA). Transduced cells were selected using 2 μg/mL puromycin (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: A total of 3-4 x 105 microglia were plated in a 6-well plate and treated with 10 μg/ml immunoglobulin IgG1 control antibody (Santa Cruz Biotechnology, sc-2025) or JAM-A-blocking antibody J10.4 (Santa Cruz Biotechnology ...
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bioRxiv - Genetics 2021Quote: ... 300 million #H5 clone mESCs were infected with a 1:10 dilution of virus-containing supernatant for 24 h in the presence of 2 μg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220) (Before infection ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... Samples were run on 10-12% SDS-PAGE and analyzed by western blotting with anti-His (SigmaAldrich, H1029) and anti-GST (Santa Cruz Biotechnology, sc-138) antibodies ...
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bioRxiv - Pathology 2021Quote: ... After blocking with donkey serum (10%, for 30 min at room temperature) slides were incubated overnight at 4°C with anti-AQP2 antibody (Santa Cruz, sc-9882, 1/400) and anti-P-p44/42 MAPK (T202/Y204 antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated with 1~10 μg/ml (according to the manufacturer manual) primary antibodies towards proteins of interest (PCNA (Santa Cruz, mouse monoclonal, sc-56), H3K27ac (Millipore ...
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bioRxiv - Genomics 2022Quote: ... The rest of the supernatants was subjected to overnight immunoprecipitation with 10 μg of IgG (Milipore, 12-371B; 1 μg/μl) or SF3B1 antibody (Santa Cruz, sc-514655; 0.2 μg/μl). Next day ...
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bioRxiv - Cancer Biology 2023Quote: ... and then frozen at -80°C for later use or applied directly to cells with 4-10 μg/mL polybrene (Santa Cruz Biotechnology, Inc., cat no. SC-134220). The culture media on target plates was changed 24 hours post- transduction.