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Citations for Santa Cruz :
701 - 750 of 750 citations for Hydrogen Peroxide Fluorescent Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... placed in a blocking solution (0.5% TX-100 in PBS + 3% NDS) and then incubated for 2 days at 4°C in goat anti-calbindin (1:200; Cat#SC7691; Santa Cruz Biotechnology, Dallas, Texas), followed by 2.5 hours incubation at RT of secondary antibody coupled to a green fluorophore (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed and incubated at room temperature for 2 hours with the appropriate HRP-conjugated secondary antibodies as follows: mouse anti-rabbit (1/1000 dilution; Santa Cruz; cat #sc-2357) and mouse IgGκ BP (1/1000 dilution ...
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bioRxiv - Pathology 2019Quote: ... Cell lysates of equal protein amounts were incubated overnight at 4°C (constant rotation) with 2 μg of an anti-HA antibody or immunoglobulin G (IgG) control (sc2027; Santa Cruz Biotechnology, Dallas, TX). Co-immunoprecipitation of STAT3 with associated proteins was then performed by using Protein A/G Agarose beads included in the Pierce Classic IP Kit (26146 ...
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bioRxiv - Immunology 2019Quote: ... the membrane was re-blocked with 5% BSA in 1x TBST for 2 hour at room temperature before probing with a 1:200 dilution anti-vinculin 7F9 (Santa Cruz Biotechnology; sc-73614) in 5% BSA/1x TBST for 1 hour at room temperature followed by anti-mouse IgG HRP secondary ...
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bioRxiv - Genetics 2021Quote: ... 300 million #H5 clone mESCs were infected with a 1:10 dilution of virus-containing supernatant for 24 h in the presence of 2 μg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220) (Before infection ...
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bioRxiv - Plant Biology 2022Quote: ... It was then probed by an anti-GFP mouse monoclonal antibody (1:600, clone B-2; sc-9996, Santa Cruz Biotechnology, Dallas, Texas, USA). Anti-mouse IgG conjugated by peroxidase (A9044 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cells were blocked in 3% BSA in PBS with 0,2% Tween 20 and incubated overnight with anti-MYC (A-14, Santa Cruz Biotechnology, 2 ug/ml), anti-CENP-C (PD030 ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were removed from dishes and assessed by flow cytometry for construct expression and selected in puromycin 2 μg/mL (Santa Cruz Biotech, sc-108071B). Phoenix-Eco or Phoenix-Eco alpha-V beta-3 were transfected with PB-vector constructs as above ...
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bioRxiv - Immunology 2023Quote: ... Lungs were dissected at the stated time post tumor initiation and fixed for 24 hours in 2% paraformaldehyde (Santa Cruz Biotechnology Cat# sc-253236) at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... were used to produce HCT116 H2B-GFP ABAT knock down cells at a multiplicity of infection (MOI) of 5 and 2 mg/mL of puromycin (Santa Cruz Biotechnology, #sc-108071) was used to select for knocked down cells ...
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bioRxiv - Developmental Biology 2024Quote: ... Antibodies used for immunofluorescence were Rat Anti-C-peptide (Developmental Studies Hybridoma Bank, ID-4) and Goat Anti-GLP-2 (Santa Cruz Antibodies, C-20).
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bioRxiv - Biophysics 2021Quote: ... The primary antibodies used for the immunostaining were mouse monoclonal antibodies (Arp3 [A-1], sc-48344; formin 2 [C-3], sc-376787; Santa Cruz Biotechnology, Dallas, Texas, USA). The primary antibodies were diluted 1:200 in 3% BSA and left in contact with the cells for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... for 30 min at RT and incubated with the following primary antibodies at 4°C overnight in TBST with 5% BSA: monoclonal mouse GFP antibody (B-2: sc-9996, Santa Cruz Biotechnology, Inc., 1:500), polyclonal pan-XIRP2 antibody (custom generated (see above) ...
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bioRxiv - Cancer Biology 2019Quote: ... The blots were blocked with 3% bovine serum albumin in TBS-T for 2 hours and incubated overnight with BAX (mouse monoclonal, Santa Cruz Biotechnology, Dallas, TX, USA) and Bcl-2 (mouse monoclonal ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were incubated with Lamp1anti-goat primary antibody (1:250, sc-8099, Santa Cruz Bio-technology; Santa Cruz, CA, USA; 2 h, 4 °C) in the blocking buffer for 45 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing IgG to the SARS-CoV-2 nucleoprotein (NP) and with mouse anti-human GAPDH monoclonal antibody (G-9: Santa Cruz Biotechnology, Dallas, TX, USA). The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma ...
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bioRxiv - Genomics 2021Quote: ... iPSC-derived MSCs were cultured to at least passage 6 and seeded at 4.2 × 104 cell/cm2 in culture dishes (4-10cm diameter) coated in 5ug/cm2 Type I Collagen (sc-136157, Santa Cruz Biotechnology, Dallas, TX, USA). After culturing cells for 1 day in MSC medium ...
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bioRxiv - Biophysics 2020Quote: ... A mixture of 0.5 µm Alexa Fluor 488 lysine labeled NSARS-CoV-2 and 1:100 Alexa Fluor 594 conjugated G3BP1 antibody (Santa Cruz Biotechnology, sc-365338 AF594) in cell permeabilization buffer was added to the cells and movies were recorded with 512 x 512 pixel resolution at 1000 Hz speed and 1 s per frame for about 2-3 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was incubated for 1h at room temperature in blocking buffer (5% nonfat milk in PBS pH 7.4 containing 0.1% Triton X-100 and 0.02% NaN3) and immunoblotted for 2 h at room temperature with antibodies raised against insulin (Santa Cruz Biotechnology Inc, Santa Cruz, CA), the porosome associated protein ATP2C1 and actin (Santa Cruz Biotechnology Inc ...
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bioRxiv - Molecular Biology 2022Quote: ... 1/10000) (Zhu et al. 2010), anti-Red1 (chicken, 1/3000) (Shinohara et al. 2008) and anti-α-tubulin (YL1/2, Santa Cruz Biotechnology, 1/3000). Tubulin signals were visualized using the LI-COR Bioscience system or alkaline phosphatase stain (BCIP-NBT solution kit ...
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies were used in the indicated dilutions: anti-α-tubulin (1:1000, B-5-1-2, Santa Cruz, USA, cat no sc-23948), anti-β-actin (1:10000 ...
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bioRxiv - Physiology 2021Quote: ... Kidney cortex cryosections were labeled with a polyclonal goat anti-aquaporin-2 (Aqp2) antibody (0.2 μg/ml, sc-9882; Santa Cruz Biotechnology, Santa Cruz CA, USA), and detected using a donkey anti-goat immunoglobulin G conjugated to Cyanine Cy3 (0.5 μg/ml ...
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bioRxiv - Biochemistry 2019Quote: ... The primary antibody used to detect UCP2 was 1:2000 dilution of anti-UCP1/2/3 raised in rabbit (Santa Cruz Biotechnology Inc. Dallas, Texas), and for AAC1 the primary antibody was 1:5000 dilution of rabbit IgG anti-AAC1 (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... On the day of the transfection two separate solutions were prepared: Solution A was made by adding 2 μg of plasmid DNA (Santa Cruz Biotechnology ®, sc-405646) into 130 μl of plasmid transfection medium (Santa Cruz Biotechnology ® ...
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bioRxiv - Developmental Biology 2022Quote: Primary antibodies used in this study are as follows: rabbit anti-CTP synthase 1/2 (y88) (1:1000, Santa Cruz Biotechnology, Cat. No. sc-134457) and mouse anti-HTS (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Whole cell lysates of Th2 cells were prepared and immunoprecipitated with 2 µg of pulldown antibodies (Gata3, VDR, Gfi1) using protein G agarose beads (Santa Cruz Biotechnology, Dallas, TX, USA). Eluted conjugates were run on SDS-PAGE and probed with primary antibodies of partner proteins ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated overnight at 4◦C or for 1 h at RT in 2%BSA PBS containing primary antibody mouse anti-lamin B1 (Santa Cruz, sc-365214, 1/500). Cells were washed using PBS and incubated for 1 h at room temperature with secondary antibody in 2% BSA PBS ...
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bioRxiv - Biophysics 2024Quote: ... TDP-43 aggregation levels were detected by GFP fluorescence signal and immunoblotting for GFP using α-GFP (B-2) primary antibody (Santa Cruz Biotechnology, sc-9996) with α-mouse IgG secondary antibody (MBL).
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were detected by immunoblotting with a chemiluminescence Luminol kit (SC-2048, Santa Cruz Biotechnology) using a LumiAnalyst Imager (Roche Applied Sciences).
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bioRxiv - Biochemistry 2021Quote: ... total RNA was collected from cells using an RNA isolation kit (Zymo, Santa Cruz, CA). Total RNA underwent reverse transcription (RT ...
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bioRxiv - Immunology 2022Quote: Apoptosis in FLS cultures was measured using a commercial flow cytometry kit (Santa Cruz biotechnology) for Annexin V-FITC binding to phosphatidylserine [28] ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were incubated with primary antibodies in 2% BSA in PBS overnight at 4°C: mouse anti-PPARγ (Santa Cruz Biotech, sc-7273, 1:1,000), rabbit anti-CEBPα (Santa Cruz Biotech ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were subsequently incubated with primary antibodies against Pax7 (Developmental Studies Hybridoma Bank, 2 μg/mL) and MyoD (Santa Cruz Biotechnology, clone G-1, 1:200) diluted in 2.5% BSA and 2.5% GS in PBST ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated at 4°C overnight with 2 μg/ml each of anti-ERα(H222) rat IgG1 monoclonal antibody (mAb) (Santa Cruz Biotech, sc-5349, 1:100) and HA-probe (F-7 ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were incubated with primary antibodies in 2% BSA in PBS overnight at 4°C: mouse anti-PPARγ (Santa Cruz Biotech, sc-7273, 1:1,000), rabbit anti-CEBPα (Santa Cruz Biotech ...
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bioRxiv - Immunology 2023Quote: Chromatin from flow-sorted small pre-B cells (2×106) from WT and Brwd1-/- mice were used for each ChIP experiment with anti-BRWD1 (E-15, Santa Cruz Biotechnology, sc-83517, lot J1508), anti-H3K4me1 (07-436 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2, sc-233X, Santa Cruz; POU2AF1, sc-955X, Santa Cruz; p300, A300-358A, Bethyl). ATAC-Seq was performed as previously described10 with nuclei isolated from 50,000 cells for each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% CO2.GIV knock-out (KO) cell lines were generated using Pooled guide RNA plasmids (commercially obtained from Santa Cruz Biotechnology; Cat# sc-402236-KO-2), as described earlier(67) ...
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bioRxiv - Biophysics 2023Quote: ... a commercial CRISPR/Cas9-based gene editing kit was employed (Santa Cruz Biotechnology, cat. sc-416469-NIC) including constructs expressing Cas9-D10A nickase ...
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Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genesbioRxiv - Plant Biology 2022Quote: ... sRNA libraries were constructed using the RealSeq-AC miRNA Library Kit for Illumina sequencing (Somagenics, Santa Cruz, USA) using an input of 150 ng total RNA and 16 PCR amplification cycles ...
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miR-206 inhibits estrogen-induced proliferation and invasion of ER-α36 positive gastric cancer cellsbioRxiv - Cancer Biology 2021Quote: ... The protein concentration was obtained with Bicinchoninic Acid (BCA) Protein Assay Kit (Santa Cruz Biotechnology, Inc., Dallas, TX USA). For Western blot analysis ...
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bioRxiv - Biophysics 2023Quote: ... were conjugated using the kit protocol to an anti-RADX mouse monoclonal Ab (CXorf57, Santa Cruz Biotechnology, sc-514563). The conjugated antibody was then incubated with an excess of RADX for 30 min on ice ...
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bioRxiv - Plant Biology 2020Quote: ... Small RNA libraries were prepared using the RealSeq-AC miRNA Library Kit for Illumina sequencing (Somagenics, Santa Cruz, CA, USA), following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2023Quote: ... The fixed chromatin was extracted from the treated gametophytes using the DovetailTM Hi-C Kit (Dovetail Genomics, Santa Cruz, CA). After digestion with DpnII ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were washed and incubated in a biotinylated secondary antibody followed by streptavidin-HRP (ABC kit Santa Cruz Biotechnology; Texas, USA). Detection was done using hydrogen peroxidase as substrate and 3 ...
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bioRxiv - Neuroscience 2022Quote: The SPPL2b gene was knocked out in HEK293 WT cells by using the SPPL2b CRISPR/Cas9 Knockout Plasmid kit from Santa Cruz Biotechnology® (sc-405646) ...
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bioRxiv - Cell Biology 2020Quote: ... The slides were then incubated with goat anti-rabbit secondary antibody and visualized using Diaminobenzidine (DAB) staining kit (Santa Cruz Biotechnology, Santa Cruz, USA) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... constructs via lentiviral particles according to the kits instructions (Cat# sc-40256-V, Cat# sc-44313-V and Cat# sc-29487-V, Santa Cruz Biotechnology, USA,). Control samples were transduced using nonspecific scrambled shRNA control constructs (Cat# sc-108060 ...
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bioRxiv - Cell Biology 2020Quote: ... The slides were then incubated with goat anti-rabbit secondary antibody and visualized using Diaminobenzidine (DAB) staining kit (Santa Cruz Biotechnology, Santa Cruz, USA) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: Monocytes were washed and resuspended in 100 ul of nucleofector solution provided with the Amaxa Noclecfector Kit V and transfected separately with siRNA-n-SMase (Santa Cruz Biotechnology, INK. SC-106277), scramble (control ...