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Citations for Santa Cruz :
4801 - 4850 of 5271 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... Selected cryo-sections were then incubated overnight with the following primary antibodies: anti-Shox2 (1:300, Santa Cruz JK-6E, sc-81955), anti-SMA-Cy3 (1:250 ...
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bioRxiv - Bioengineering 2023Quote: ... Alomone Labs) or 5 µM of antibodies for targeting neurons with dopamine receptor 2 (D2R) (anti-D2DR, sc-5303, Santa Cruz Biotechnology) or without cell type specificity (rabbit anti-human IgG ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were further incubated at 37°C for 1 hour with horseradish peroxidase-conjugated secondary antibodies: goat anti-rabbit (sc-2004, 1:5000, Santa Cruz Biotechnology) or goat anti-mouse (sc-2005 ...
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bioRxiv - Cancer Biology 2024Quote: ... were subjected to a secondary incubation with an Anti-rabbit IgG horseradish peroxidase coupled antibody (1:2,000, sc-516102, Santa Cruz Biotechnology, Germany) and an anti-mouse IgG horseradish peroxidase coupled antibody (1:2,000 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with a primary antibody targeting Ankyrin G overnight (rabbit anti-AnkG, 1:500, H215, Santa Cruz Cat no: sc28561) followed by 2 hours incubation in the secondary antibody (Alexa FluorTM 568 donkey anti-rabbit ...
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bioRxiv - Microbiology 2024Quote: ... and the remaining three aliquots were immunoprecipitated overnight at 4 °C with the indicated antibody mixed with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology). Then ...
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bioRxiv - Bioengineering 2024Quote: ... cells were cocultured with 1:150 primary antibodies (anti Gal-1, sc-166618, Santa Cruz Biotechnology Inc.; anti-Gal-3, sc-32790, Santa Cruz Biotechnology Inc ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were fixed using 4% PFA and IF staining was performed using the following primary antibodies: mouse mAb α-LT Cm2B4 (1:1000; Santa Cruz), rabbit mAbs α-VP1 (1:500 ...
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bioRxiv - Systems Biology 2024Quote: ... cells were identified by post-hoc immunostaining for insulin with a rabbit anti-insulin primary antibody (Santa Cruz; #SC-9168; RRID: AB_2126540) and goat anti-rabbit Alexa Fluor488 secondary (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... CH58 and CH59 were used as primary antibodies at 1:5000 dilution in PBS and rabbit anti-human Ab HRP conjugate (Santa Cruz Biotechnology) was used as secondary antibodies at 1:10,000 dilution in PBS ...
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bioRxiv - Biophysics 2023Quote: ... a commercial CRISPR/Cas9-based gene editing kit was employed (Santa Cruz Biotechnology, cat. sc-416469-NIC) including constructs expressing Cas9-D10A nickase ...
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bioRxiv - Cancer Biology 2021Quote: ... the extracted lysates were first pre-cleared with protein G-magnetic beads at 4 °C for 2 h followed by incubation with HOXB13 antibody (2 μg/sample, Santa Cruz, sc-28333) overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein extracts were incubated with each antibody with rotation for 2 hr and then added to protein A/G agarose beads (Santa Cruz, sc-2003) for an additional 1 hr ...
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bioRxiv - Cell Biology 2020Quote: ... blocked for 30 min with BSA (1%) in PBS and incubated for 60 min with anti-acetylated-α-tubulin antibody (Santa Cruz Biotechnology) to identify primary cilia.24 Cells were counter-stained with DAPI to locate cell nuclei and mounted with Vectashield mounting medium (Vector Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... by washing 3 times with phosphate-buffer saline (PBS) followed by incubation with either 10 µg of mouse monoclonal anti-Ndc80/Hec1 antibody (Santa Cruz, sc-515550) or 10 µg of mouse IgG (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were incubated overnight at 4°C with primary antibodies (1:100 dilution) against GATA4 (sc-25310, Santa Cruz Biotechnology, Santa Cruz, CA), BRACHYURY (sc-20109 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were incubated overnight at 4°C with primary antibodies (1:100 dilution) against GATA4 (sc-25310, Santa Cruz Biotechnology, Santa Cruz, CA), BRACHYURY (sc-20109 ...
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bioRxiv - Cell Biology 2020Quote: ... slides were briefly rinsed with PBS and stained overnight at 4°C with antibodies (mouse anti-EPS8, BD Transduction Laboratories Cat#610144, 1:200; rabbit anti-Villin 1:50, Santa Cruz Cat# SC28283) in 1% NGS in PBS ...
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bioRxiv - Cancer Biology 2020Quote: A previously published immunofluorescence-fluorescence in situ hybridization protocol was carried out on cells seeded on sterile glass coverslips and tumor sections.43 Coverslips were incubated with PML antibody (Santa Cruz, sc-5621) diluted 1:200 for one hour at room temperature ...
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bioRxiv - Genetics 2019Quote: ... the cells were stained according to the same protocol except for the myc staining that was performed using an unconjugated anti-myc tag antibody (Santa Cruz Biotechnology, 9E10) followed by an AMCA anti-mouse antibody (Jackson ImmunoResearch).
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bioRxiv - Microbiology 2022Quote: ... was incubated with 25 μl of agarose-coupled anti-CDK1 or anti-CycA2 antibodies (sc-54AC or sc-239AC, Santa Cruz Biotechnology, respectively) for 2 h at 4 °C with gentle rocking ...
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bioRxiv - Neuroscience 2021Quote: ... The tissues sections were then incubated with primary antibody (rabbit anti-c-Fos, SC-52; Santa Cruz Biotechnology, 1:1000 in PBS-T) at 4°C for 72 hrs ...
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bioRxiv - Molecular Biology 2020Quote: Mouse monoclonal primary antibodies included: anti-CD63 (clone MX-49.129.5, 2 μg/mL, cat. no. sc-5275, Santa Cruz Biotechnology, Dallas, TX, USA), anti-CD81 (clone 1.3.3.22 ...
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bioRxiv - Molecular Biology 2021Quote: ... The primary antibodies used for incubation were anti-TGFβ 1/2/3 [H-112] (dilution 1: 80, Santa Cruz Biotechnology, Inc., sc-7892), anti-COX2 antibody [EP1978Y] (dilution 1 ...
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bioRxiv - Genomics 2020Quote: ... Drug-resistant colonies were isolated and expression of TIR1-9myc was confirmed by Western blot using anti-Myc antibody (Santa Cruz, sc-40). Clones expressing high level of TIR1-9myc were used for the subsequent experiments.
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bioRxiv - Developmental Biology 2020Quote: ... membranes were rinsed several times in 1xTBSTw and incubated with goat anti-rabbit HRP-conjugated secondary antibody (1:20,000; Bio-Rad, catalog 179-5046; or 1:10,000; Santa Cruz, catalog sc-2004) in 5% non-fat dried milk for 1 hour at room temperature with gentle agitation ...
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bioRxiv - Neuroscience 2019Quote: ... permeabilized in PBS with 10% triton-x for 30 min and incubated for three days at 4 °C with goat anti-DCX antibody (1:250 in 10% triton-x and 3% horse serum (sc-8066; Santa Cruz Biotechnology, USA). Sections were washed and incubated in biotinylated donkey anti-goat secondary antibody for 60 minutes (1:250 ...
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bioRxiv - Cell Biology 2019Quote: ... membranes were washed three times for 15 min with PBST and incubated with the secondary antibody donkey anti-rabbit- IgG-HRP (sc-2317, Santa Cruz Biotechnology, USA) at 1:10000 for 1h at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... The membranes were then blocked with 5% non-fat milk in TBST 0.1% and immunoblotted overnight at 4°C with the following primary antibodies: TCHP (Santa Cruz Biotechnology, SC-515025), β-actin (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg of total membrane protein in membrane vesicles of MQ614 harboring the indicated MhsT variant (or the control plasmid) were subjected to 11 % SDS-PAGE followed by incubation of the membrane with the His probe antibody (Santa Cruz Biotechnology, Inc.) and horseradish peroxidase-based chemiluminescence detection (SuperSignal® West Pico kit ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were probed with anti HA-tag monoclonal antibody (Covance, HA.11, 1:100) while β-Tubulin (Santa Cruz, H-235, 1: 500) was used as a loading control ...
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bioRxiv - Bioengineering 2019Quote: ... Sections were stained overnight at 4°C using unconjugated primary antibodies at 1:100 dilution for endomucin (rat anti-mouse sc-65495, Santa Cruz Biotechnology, Santa Cruz, CA) and at 1:200 dilution for collagen type I (rabbit anti-mouse ...
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bioRxiv - Physiology 2019Quote: ... slides were incubated overnight at 4°C with the following antibodies (diluted in blocking buffer): Anti PECAM-1 (sc-376764, Santa Cruz, 1:50), anti Ki67 (AB9260 ...
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bioRxiv - Physiology 2019Quote: ... sections were incubated with horseradish peroxidase (HRP)-conjugated donkey anti-goat or goat anti-rabbit secondary antibody (R&D system and Santa Cruz), before incubation with diaminobenzidine substrate as a chromogen (Dako ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were permeabilized with 0.5% Triton-X 100 and blocked with 1% BSAprior to incubation with primary and secondary antibody (1:100) (Nox1 (Santa Cruz, sc-5821), (LRRC8D (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: The following commercially available antibodies were used at the indicated concentrations for western blot: Anti-ZFP207 (Santa Cruz Biotechnology, sc-271943, 1:500), anti–β-ACTIN (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Brain sections were then incubated in a goat polyclonal anti-OXA primary antibody (1:10,000 dilution; SC-8070, Santa Cruz, Dallas, TX, USA) followed by biotinylated horse anti-goat IgG secondary antibody (1:1,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... PDE4 (kindly provided to us by Kenneth Miller, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma), PP1 (sc6104, 1:1000) or an anti-calstabin antibody (Santa Cruz 1: 2,500). To determine channel oxidation ...
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bioRxiv - Biochemistry 2021Quote: ... SEC experiments were analyzed by ELISA as described below with antibodies to EWSR1 (clone C-9, Santa Cruz Biotechnology cat. no. sc-48404) and RNA Pol II (clone CTD4H8 ...
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bioRxiv - Bioengineering 2021Quote: ... the membrane was washed three times with Tris buffer and incubated for one hour with anti-mouse antibodies conjugated with horseradish peroxidase (Santa Cruz Biotechnology, USA). After washing the membrane ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were reprobed for Gβ by stripping and then incubating overnight with 1:200 diluted mouse monoclonal anti-Gβ antibody (Santa Cruz #sc-166123) followed by a secondary incubation with 1:1000 diluted m-IgGk BP-HRP (Santa Cruz #sc-516102) ...
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bioRxiv - Bioengineering 2020Quote: ... Scaffolds were incubated overnight with antibodies against myosin heavy chain 4 (MF20, mouse monoclonal, eBioscience, 1:200) or MyoD (mouse monoclonal, Santa Cruz, 1:200) in antibody diluent (BD Biosciences) ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were incubated overnight at 4°C with the primary antibody goat polyclonal anti-GFAP 1:1000 (Santa Cruz, Dallas TX, USA) or rabbit anti-Actin 1:1000 (Santa Cruz ...
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bioRxiv - Neuroscience 2020Quote: ... an anti-Zif268/Egr1 rabbit antibody (1:1500 for DAB staining; 1:300 for fluorescence staining; C-19, Santa Cruz Biotechnology, Dallas, USA), and anti-mCherry animal antibody (1:1000 for fluorescence staining ...
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bioRxiv - Neuroscience 2021Quote: ... low-fat milk in TBST for 1 hour at room temperature and subsequently incubated with POPDC1 antibody (sc-49889, Santa Cruz Biotechnology Inc.) overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: The pH of 500 μl mouse IgG1 HPV 16 E6/18 E6-HRP (CP15) monoclonal antibodies (Santa Cruz Biotechnologies, USA Cat# sc-460) was determined using a pH Meter ...
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bioRxiv - Cancer Biology 2020Quote: BCR-ABL protein expression was assessed by SDS-PAGE and western blotting using a mouse anti-ABL antibody (1:1,000, Santa Cruz Biotechnology, CA, USA). Horseradish peroxidase-conjugated α-mouse antibody (1:10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with one of the following anti-human primary antibodies diluted in blocking buffer: goat PAX3 (1:100; 4°C overnight; Santa Cruz, sc-34916), mouse TYRP1 (MEL-5 ...
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bioRxiv - Neuroscience 2019Quote: ... in PBS for 20 min at room temperature (RT) and incubated with rabbit polyclonal antibodies against ATF3 (Santa Cruz, 1:500, RRID: AB_1078233), and Horseradish peroxidase (HRP)-conjugated mouse monoclonal antibody against GAPDH (Cell Signaling ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were incubated with goat anti-mouse HRP conjugated secondary antibodies (Novex, A16072) and visualized using luminol reagent (Santa Cruz Biotechnology, sc-2048). Membranes were scanned using the GE LAS 4000 imager (GE Healthscience ...