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Citations for Santa Cruz :
401 - 450 of 489 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Transfected cells were washed with HBSS and lysed in-well with radioimmunoprecipitation assay (RIPA) buffer (Santa Cruz, Dallas, TX) supplemented with Halt Protease Inhibitor (Pierce ...
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bioRxiv - Cell Biology 2020Quote: Cells were lysed on ice using a radioimmunoprecipitation assay (RIPA) lysis buffer system (Santa Cruz Biotechnology, Dallas, TX, USA) containing PMSF ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse anti-His (1:500 for immunostaining and 1:1000 for Western blotting assay, Santa Cruz Biotechnology, #sc-53073), rabbit anti-HA (1:1,000 ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg of total membrane protein in membrane vesicles of MQ614 harboring the indicated MhsT variant (or the control plasmid) were subjected to 11 % SDS-PAGE followed by incubation of the membrane with the His probe antibody (Santa Cruz Biotechnology, Inc.) and horseradish peroxidase-based chemiluminescence detection (SuperSignal® West Pico kit ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were incubated overnight at 4°C with the primary antibody goat polyclonal anti-GFAP 1:1000 (Santa Cruz, Dallas TX, USA) or rabbit anti-Actin 1:1000 (Santa Cruz ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were incubated with goat anti-mouse HRP conjugated secondary antibodies (Novex, A16072) and visualized using luminol reagent (Santa Cruz Biotechnology, sc-2048). Membranes were scanned using the GE LAS 4000 imager (GE Healthscience ...
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bioRxiv - Immunology 2022Quote: ... The immunoprecipitated samples were transferred onto PVDF membranes as described earlier and immunoblotted with HRP-conjugated anti-Fyn mAb (clone 15, Santa Cruz Biotechnology, Santa Cruz, CA) and anti-Lck mAb (clone 3A5 ...
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bioRxiv - Genetics 2020Quote: ... Section of the membrane with 75-50 kDa proteins was incubated with rat monoclonal antibodies against yeast α-tubulin (sc-53030, Santa Cruz Biotechnology) at 1:2,000 – 1:4,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed with TBS and incubated with horseradish peroxidase-conjugated anti-rabbit IgG (1:5000, Santa Cruz Biotechnologies, Dallas, TX, EUA) for 2 hours ...
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bioRxiv - Immunology 2020Quote: ... Membranes were incubated with Luminata Forte Western HRP Substrate (MilliporeSigma) for five minutes and developed on UltraCruz® Autoradiography films (Santa Cruz Biotechnology). Membranes were stripped with RestoreTM Western Blot Stripping Buffer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: All the protein samples were separated by sodium dodecyl sulfate polyacrylamide (SDS)-PAGE and were transferred to nitrocellulose membrane (Bio-Rad laboratories, Genesee Scientific, Cytiva, and Santa Cruz Biotechnology) and incubated with primary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Membrane was blocked for one hour in 5% milk or TBS-T and incubated at RT with anti-Runx1 (Santa Cruz SC-365644X), anti-ELF1 (Santa Cruz SC-133096X ...
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bioRxiv - Molecular Biology 2024Quote: ... transferred overnight onto a fluorescent membrane, blotted for rabbit anti-KDM5C (in house, 1:500) and mouse anti-DAXX (Santa Cruz #(H-7): sc-8043 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated by electrophoresis on 10% or 12% gels and transferred to PVDF membranes that were probed with anti-Bmp4 (1:1000, Santa Cruz RRID: AB_2063534), anti- pSmad1/5 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... kit (Santa Cruz Biotechnology, sc-13508), Erk (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-Myc (1:1,000 for western blotting and 2 μg for co-IP assays; sc-40; Santa Cruz Biotechnology), and mouse anti-Flag (1:1,000 for western blotting and 2 μg for co-IP assays ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were quenched by placing the assay tubes on ice and adding 10 μM inhibitor L-685,458 (Santa Cruz Biotechnology). De novo Aβ40 production was quantified by MSD ELISA as described previously48 ...
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bioRxiv - Molecular Biology 2023Quote: RNase and ATPase assays were performed as described in the presence or absence of guanabenz acetate (sc-203590, Santa Cruz).
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bioRxiv - Cancer Biology 2024Quote: Cells were washed with ice-cold phosphate-buffered saline (PBS) then lysed with radioimmunoprecipitation assay lysis buffer (Santa Cruz Biotechnology) supplemented with phosphatase inhibitor cocktail 2 (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Next the membranes were washed for 5 min X 4 times with PBST and incubated with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology, SantaCruz, CA) for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... and 30 μg/lane of sample were separated by 4–15% gradient Trisacetate SDS-PAGE and electro transferred to PVDF membrane (Santa Cruz Biotechnology, sc-3723). The blots were probed with individual primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... nitrocellulose membranes were incubated for 1 h at room temperature with the horseradish peroxidase conjugated IgG (1:5000; Santa Cruz Biotechnology, Inc, CA, USA). The bands were visualized by the SuperSignal West Pico Chemiluminescent Substrate Trial Kit (Pierce ...
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bioRxiv - Molecular Biology 2022Quote: Proteins were separated by SDS-PAGE (7.5% or 10% gels) followed by immunoblot analysis on Immobilon-P PVDF membranes (MilliporeSigma; IPVH00005) using mouse monoclonal α-Myc antibody (9E10; Santa Cruz; sc-40), α-HA (16B12 ...
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bioRxiv - Genetics 2022Quote: ... 20% of each sample was resolved by SDS-PAGE gels and transferred to nitrocellulose membranes for immunoblotting with anti-HA (Santa Cruz, clone F-7) to detect Stu2 or anti-AcK (Genetex ...
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bioRxiv - Physiology 2023Quote: ... solution for 1.5 hours at room temperature the membranes were incubated overnight at 4°C with the following primary antibodies: insulin receptor (IR; 1:100, Santa Cruz Biotechnology, Madrid, Spain), protein kinase B (Akt ...
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bioRxiv - Biochemistry 2023Quote: ... the membranes were incubated with primary antibody either 1:500 mouse anti-angiogenin monoclonal antibody C-1 (sc-74528, Santa Cruz Biotechnologies, Dallas, TX) or 1:500 mouse anti-ribosomal protein s5 monoclonal antibody (A-8 ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed twice with DPBS to wash out the non-membrane bound GFP-LC3B out of the permeabilized cells and fixated with 4% paraformaldehyde (PFA) (Santa Cruz Biotechnology, #sc-281692). The mean fluorescence intensity (MFI ...
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bioRxiv - Neuroscience 2021Quote: ... These total or lysosomal lysates were diluted in 5μl activity assay buffer (see above) or 5μl of 20mM Conduritol B Epoxide (CBE) (Santa Cruz, sc-201356A) (specific GCase inhibitor ...
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bioRxiv - Plant Biology 2020Quote: ... Detection was carried out by chemiluminescence assays after incubation with an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (Sigma or Santa Cruz).
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bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used in PLA assays: mouse anti p27 (SX53G8.5, sc-53871), LAMP1 (E-5, Sc-17768), rabbit anti p27 (C19, sc-528) (Santa Cruz Biotechnology), rabbit anti LAMTOR1 (#8975) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was collected and then the protein concentration of each sample was determined by bicinchoninic acid assays (Santa Cruz Biotechnology). Diluted sample and reaction buffer were added into 96-wells plate containing menadione or isoplumbagin with cofactor NADH and water-soluble tetrazolium salt [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] reagent ...
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bioRxiv - Neuroscience 2022Quote: ... Spinal cord tissue was homogenized by mechanical trituration followed by sonication in radio-immunoprecipitation assay (RIPA) lysis buffer (1 ml; Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2022Quote: ... The immunoprecipitated samples were transferred onto PVDF membranes as described earlier and immunoblotted with HRP-conjugated anti-Fyn mAb (clone 15, Santa Cruz Biotechnology, Santa Cruz, CA) and anti-Lck mAb (clone 3A5 ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was incubated 1 h with the horseradish peroxidase conjugated donkey anti-goat IgG (Santa Cruz sc2020; 1/2000 in TBS-T + 3%BSA). After 5 washes of 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was washed with TBST buffer for three times and incubated with the goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology, Cat# sc-2004) with 1:3000 dilution at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were incubated with the following primary antibodies: a rabbit polyclonal antibody against ANP (N-20, 1:500, Santa Cruz Biotechnology, Dallas, TX, USA), sarcolipin (ABT13 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... After washing in TBST the membranes were incubated in a secondary antibody with HRP (anti-rabbit, Vector pI-1000, 1:5000; anti-mouse, Santa Cruz, sc-2005, 1:5000) and washed again ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was washed with TBST buffer and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit (Santa Cruz, CA, Cat# SC-2004)/anti-mouse antibodies (Santa Cruz ...
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bioRxiv - Physiology 2023Quote: ... the membranes were incubated overnight at 4°C with the primary antibodies against PGC-1α (1:1000; 92 kDa; Santa Cruz Biotechnology INC, Texas, EUA), PPARγ (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to PVDF membrane (Amersham 0.2 Hybond P, Cytiva) and revealed using following antibodies: mouse AGR2 (Santa Cruz Biotechnology, Clone 6C5, 1/1000), rabbit IRE1β (gift from David Ron ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated with horseradish peroxidase–conjugated secondary antibodies (1:4000: Cell Signaling Technology; 1:2,000: R&D Systems, Bio-Techne; Santa Cruz Biotechnology) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... equal protein loading was controlled by re-probing the membrane with anti–β-actin-HRP antibody (1:1,000 dilution; Santa Cruz Biotechnology #sc-47778-HRP). Chemiluminescence was detected using the ChemiDoc MP Imaging System (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... the blotted membranes were incubated overnight at 4 °C with primary antibodies of anti-c-Myc (1:500) (sc-40, Santa Cruz Biotechnology, Dallas, TX, USA), GAPDH was used as a loading control (G-9 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated on a shaker with the primary antibodies: rabbit polyclonal anti-Trk(C14) (1:1 000, Santa Cruz Biotechnology, #sc-11, RRID: AB_632554), mouse monoclonal anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH ...
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bioRxiv - Physiology 2024Quote: ... Proteins of interest were visualised and detected in the PVDF membranes employing the primary antibodies mouse anti-GluN2B (#365597, Santa Cruz Biotechnology; 1:1000 dilution), mouse anti-α1 Na+,K+-ATPase (ATP1A1 ...
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bioRxiv - Cancer Biology 2022Quote: ... we used a kit from Santa Cruz technology ...
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bioRxiv - Biochemistry 2022Quote: ... KIT was immunoprecipitated from the supernatant with 6 μg monoclonal anti-KIT antibody (Santa Cruz Biotechnology, no. sc-13508) together with 30 μL protein G PLUS-agarose beads (Santa Cruz Biotechnology ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cell monolayer was washed with cold HBSS three times and lysed by adding 50 μL/well of 1X radioimmuno-precipitation assay (RIPA) cell lysis buffer (Santa Cruz Biotechnology). The plates were maintained at constant agitation at 4°C for 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were prepared from culture cells (washed by cold PBS) or tissues by homogenization with a radioimmune precipitation assay lysis buffer (sc-24948, Santa Cruz Biotechnology) containing HaltTM protease and phosphatase inhibitor mixture (catalog no ...
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Elimination of glutamatergic transmission from Hb9 interneurons does not impact treadmill locomotionbioRxiv - Neuroscience 2021Quote: Tissues were transferred to primary Ab (Anti-DsRed-Rabbit, Rockland antibodies and assays PA, USA 1:2000; Anti-β-gal Goat or Mouse, Santa Cruz Biotechnology ...