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• PKC modulator bryostatin-1 therapeutically targets CNS innate immunity to attenuate neuroinflammation and promote remyelination

  Payam Gharibani, et al., bioRxiv - Neuroscience 2023

  Quote: ... or mouse anti-iNOS (1:200, Santa Cruz). After the primary antibodies ...
• A single polymorphic residue in humans underlies species-specific restriction of HSV-1 by the antiviral protein MxB

  Avraham Bayer, et al., bioRxiv - Microbiology 2023

  Quote: ... then incubated with an anti-mouse 555 fluorophore (Santa Cruz). Images were captured on a high-resolution Leica Stellaris Confocal Microscope using a 63x oil objective and analyzed using ImageJ software.
• The effects of phenylalanine and tyrosine levels on dopamine production in rat PC12 cells. Implications for treatment of phenylketonuria and tyrosinemia type 1

  Peter D. Szigetvari, et al., bioRxiv - Molecular Biology 2023

  Quote: ... GAPDH (#sc-32233 Santa Cruz Biotech; 1:1000) primary antibodies and proteins were detected by incubation at a 1:5000 dilution with secondary horseradish peroxidase (HRP)- conjugated antibodies (#170-6516 ...
• The effects of phenylalanine and tyrosine levels on dopamine production in rat PC12 cells. Implications for treatment of phenylketonuria and tyrosinemia type 1

  Peter D. Szigetvari, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Pan14-3-3 (#sc-629, Santa Cruz Biotech; 1:1000), DHFR (#133546 ...
• DNA methylation governs the sensitivity of repeats to restriction by the HUSH-MORC2 corepressor

  Ninoslav Pandiloski, et al., bioRxiv - Molecular Biology 2023

  Quote: ... The following day the membrane was washed twice in TBST (15 min) and incubated (1 h, room temperature) with HRP-conjugated anti-mouse or anti-rabbit secondary antibody (Santa Cruz Biotechnology, 1:5,000) diluted in MTBST ...
• A furin-deficient HEK293 cell line suggests a role for furin trafficking in the intoxication pathway of recombinant immunotoxins based on Pseudomonas exotoxin A

  Brian D. Grossman, et al., bioRxiv - Molecular Biology 2023

  Quote: ... mouse anti-furin antibody (mAb, Santa Cruz Biotechnology: sc-133141), mouse anti-β-actin (mAb ...
• BMP7 promotes cardiomyocyte regeneration

  Chiara Bongiovanni, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and anti-ERK2 (1:50, sc-1647, Santa Cruz) were used to analyse BMP7 downstream signalling after myocardial infarction.
• Urothelium-specific expression of mutationally activated Pik3ca initiates early lesions of non-invasive bladder cancer

  Lauren Shuman, et al., bioRxiv - Cancer Biology 2023

  Quote: ... goat anti-Foxa1 (1:1000; sc-6553; Santa Cruz Biotechnology; Dallas, TX), rabbit anti-Pparγ (1:200 ...
• A co-ordinated transcriptional programme in the maternal liver supplies LC-PUFAs to the conceptus using phospholipids

  Risha Amarsi, et al., bioRxiv - Physiology 2023

  Quote: ... Histological sections were incubated overnight at 4°C with the primary antibodies (rabbit anti-human COX1, ab133319, abcam, 1:200; rat anti-mouse Endomucin, sc-5495 Santa Cruz, 1:200), then incubated with Texas Red-conjugated goat anti-Rat (TI-9400 Vector Labs ...
• Extracellular sodium regulates fibroblast growth factor 23 (FGF23) formation

  Zsuzsa Radvanyi, et al., bioRxiv - Physiology 2023

  Quote: ... and β-actin (Santa Cruz; Cat. #sc-47778X) overnight at 4°C ...
• Direct measurements of luminal Ca²⁺ with endo-lysosomal GFP-aequorin (ELGA) reveal functional IP₃ receptors

  B Calvo, et al., bioRxiv - Physiology 2023

  Quote: ... mouse anti-TOM20 (1:100; Santa Cruz). The secondary antibodies (anti-mouse Alexa Fluor 568-conjugated antibodies ...
• A new pipeline SPICE identifies novel JUN-IKZF1 composite elements

  Peng Li, et al., bioRxiv - Systems Biology 2023

  Quote: ... normal mouse IgG (Santa Cruz, sc-2025), or normal rabbit IgG (Cell Signaling Technologies ...
• An integrated mechanistic and data-driven computational model predicts cell responses to high- and low-affinity EGFR ligands

  Paul J. Myers, Sung Hyun Lee, Matthew J. Lazzara, bioRxiv - Systems Biology 2023

  Quote: ... GRB2 (Santa Cruz Biotechnology, sc-255). The following primary antibodies were used with ECL detection and were diluted in TBST ...
• An unconventional mechanism of IL-1β secretion that requires Type I IFN in lupus monocytes

  Simone Caielli, et al., bioRxiv - Immunology 2023

  Quote: ... incubated with anti-cGAS (sc-515777; Santa Cruz Biotech), anti-NLPR3 (AdipoGen #AG-20B-0014-C100 ...
• Phosphorylation-Dependent Association of WRN with RPA is Required for Recovery of Replication Forks Stalled at Secondary DNA Structures

  Alessandro Noto, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-RAD51 (Santa Cruz) and mouse anti-IdU antibody (Becton Dickinson ...
• The apoptotic, inflammatory, and fibrinolytic actions of vasoinhibin are in a motif different from its antiangiogenic HGR motif

  Juan Pablo Robles, et al., bioRxiv - Cell Biology 2023

  Quote: ... and incubated with 1:200 anti-NF-κB p65 antibodies (Santa Cruz Biotechnology ...
• The apoptotic, inflammatory, and fibrinolytic actions of vasoinhibin are in a motif different from its antiangiogenic HGR motif

  Juan Pablo Robles, et al., bioRxiv - Cell Biology 2023

  Quote: ... and incubated with 1:200 anti-NF-κB p65 antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, Cat# sc-8008, RRID:AB_628017) in 1% BSA ...
• RAD52 and ERCC6L/PICH have a compensatory relationship for genome stability in mitosis

  Beth Osia, et al., bioRxiv - Cell Biology 2023

  Quote: ... Blots were probed using antibodies against RAD52 1:500 (Santa Cruz Biotechnology #sc365341), FLAG 1:1000 (Sigma #A8592) ...
• Deciphering the spatio-temporal transcriptional and chromatin accessibility of human retinal organoid development at the single cell level

  Birthe Dorgau, et al., bioRxiv - Developmental Biology 2023

  Quote: ... After fixation using 4% paraformaldehyde (PFA; Santa Cruz Biotechnologies) organoids were cryopreserved ...
• An N-terminal delivery domain defines a new class of polymorphic T6SS effectors in Enterobacterales

  Andrea Carobbi, et al., bioRxiv - Microbiology 2023

  Quote: ... Input and output fractions were resolved on SDS-PAGE and subjected to immunoblot analysis using anti-Myc antibodies (Santa Cruz Biotechnology; sc-40) or anti-GST (Santa Cruz Biotechnology ...
• An N-terminal delivery domain defines a new class of polymorphic T6SS effectors in Enterobacterales

  Andrea Carobbi, et al., bioRxiv - Microbiology 2023

  Quote: ... and immunoblotted with anti-Myc antibodies (Santa Cruz Biotechnology; 9E10, sc-40) or anti-RNA polymerase sigma 70 (RNAp ...
• Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses

  Maggie Jing Ouyang, et al., bioRxiv - Microbiology 2023

  Quote: ... mouse monoclonal anti-influenza A virus M2 antibody (Santa Cruz Biotech, Cat# 14C2: sc-32238), and anti-mouse-HRP antibody (GE Healthcare ...
• An N-terminal delivery domain defines a new class of polymorphic T6SS effectors in Enterobacterales

  Andrea Carobbi, et al., bioRxiv - Microbiology 2023

  Quote: ... or anti-GST (Santa Cruz Biotechnology; sc-459) antibodies at a 1:1000 dilution ...
• Golgi membrane proteins YIPF3 and YIPF4 regulate turnover of the Golgi apparatus through autophagy

  Shinri Kitta, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-CALCOCO1 (mouse, sc-515670; Santa Cruz Biotechnology), anti-LC3 (rabbit ...
• Identification and functional characterization of the putative members of the CTDK-1 kinase complex as regulators of growth and development in the genus Aspergillus

  Z. Agirrezabala, et al., bioRxiv - Microbiology 2023

  Quote: ... or α-HA3x (1:1000; Santa Cruz Biotechnology) mouse monoclonal antibody cocktails ...
• Autophagy preferentially degrades non-fibrillar polyQ aggregates

  Dorothy Y. Zhao, et al., bioRxiv - Cell Biology 2023

  Quote: The following antibodies were used for immunoblotting: mouse anti-GFP (Santa Cruz), rabbit anti-GFP (Roche) ...
• Vitamin D deficiency induces erectile dysfunction: role of superoxide

  Miguel A. Olivencia, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... The following immune fluorescence staining were performed: anti-VDR (1:50, sc-13133, mouse monoclonal, Santa Cruz, Dallas, USA) followed by Alexa594-conjugated anti-mouse (1:200 ...
• Olfactory detection of viruses shapes brain immunity and behavior in zebrafish

  Aurora Kraus, et al., bioRxiv - Immunology 2023

  Quote: ... or normal Rabbit IgG (Santa Cruz Biotechnology catalog no. sc-3888) in Pierce Immunostain Enhancer (Thermo Fisher Scientific catalog no ...
• Metabolic impact of trans 10, cis 12-conjugated linoleic acid on pai transgenic mice

  Yu Rao, et al., bioRxiv - Physiology 2023

  Quote: ... followed by HPR-labelled goat antirabbit IgG (Santa Cruz Biotechnology, sc-2004). The chemiluminescent signal was developed using the SuperSignal™ West Femto substrate (ThermoFisher ...
• The differentiation and integration of the hippocampal dorsoventral axis are controlled by two nuclear receptor genes

  Xiong Yang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... mouse anti-GAPDH (1:1000, Santa Cruz, Cat # sc-32233). The following secondary antibodies were applied in the study ...
• The differentiation and integration of the hippocampal dorsoventral axis are controlled by two nuclear receptor genes

  Xiong Yang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... goat anti-Dcx (1:500, Santa Cruz, Cat # sc-8066). The following secondary antibodies were used in the study ...
• The differentiation and integration of the hippocampal dorsoventral axis are controlled by two nuclear receptor genes

  Xiong Yang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... rabbit anti-Nestin (1:200, Santa Cruz, Cat # sc-20978), goat anti-Dcx (1:500 ...
• The differentiation and integration of the hippocampal dorsoventral axis are controlled by two nuclear receptor genes

  Xiong Yang, et al., bioRxiv - Developmental Biology 2023

  Quote: ... goat anti-Lhx2 (1:200, Santa Cruz, Cat # sc-19344), goat anti-Lhx5 (1:200 ...
• Leveraging genetic diversity to identify small molecules that reverse mouse skeletal muscle insulin resistance

  Stewart W.C. Masson, et al., bioRxiv - Systems Biology 2023

  Quote: ... 14-3-3 (Santa Cruz #sc-1657; diluted 1:5000). The following day membranes were washed 3 x 10 minutes in TBS-T and incubated for 1 hour in species-appropriate fluorescent or HRP secondary antibodies ...
• ALS-related p97 R155H mutation disrupts lysophagy in iPSC-derived motor neurons

  Jacob A. Klickstein, et al., bioRxiv - Neuroscience 2023

  Quote: ... TOMM70 (sc-390545 Santa Cruz), and p62 (GP62-C Progen Biotechnik) ...
• Integrated signaling and transcriptome analysis reveals Src-family kinase individualities and novel pathways controlled by their constitutive activity

  Nikolaos Koutras, et al., bioRxiv - Immunology 2023

  Quote: ... Detection was performed using Pierce ECL Western Blotting Substrate (Thermo Fischer Scientific) and either developed on Autoradiography Film (Santa Cruz Biotechnology) or scanned using ChemiDoc MP Imaging System (Bio-Rad).
• Integrated signaling and transcriptome analysis reveals Src-family kinase individualities and novel pathways controlled by their constitutive activity

  Nikolaos Koutras, et al., bioRxiv - Immunology 2023

  Quote: ... Clarified lysates were incubated with the appropriate antibodies for 2h at 4°C and subsequently with protein A/G agarose beads (Santa Cruz Biotechnology) for 1h at 4°C ...
• Integrated signaling and transcriptome analysis reveals Src-family kinase individualities and novel pathways controlled by their constitutive activity

  Nikolaos Koutras, et al., bioRxiv - Immunology 2023

  Quote: ... anti-CD22 (clone HD39) (sc-73363) antibodies were from Santa Cruz Biotechnology ...
• Inhibition of CDK12 elevates cancer cell dependence on P-TEFb by stimulation of RNA polymerase II pause release

  Zhijia Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-p53 (Santa Cruz Biotechnology, 1:3000); anti-p21 (Santa Cruz Biotechnology ...
• Inhibition of CDK12 elevates cancer cell dependence on P-TEFb by stimulation of RNA polymerase II pause release

  Zhijia Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The following antibodies were used according to manufacturers’ instructions: anti-CDK9 (Santa Cruz Biotechnology, 1:4000); anti-p53 (Santa Cruz Biotechnology ...
• Elucidation of antiviral mechanisms of natural therapeutic molecules Herbacetin and Caffeic acid phenethyl ester against chikungunya and dengue virus

  Mandar Bhutkar, et al., bioRxiv - Microbiology 2023

  Quote: ... cells were incubated with antibodies against CHIKV and DENV (anti-alphavirus 1:100, SANTA Cruz Biotechnology Inc ...
• Nuclear damage in LMNA mutant iPSC-derived cardiomyocytes is associated with impaired lamin localization to the nuclear envelope

  Melanie Wallace, et al., bioRxiv - Cell Biology 2023

  Quote: ... The Lamin A/C antibody used (Santa Cruz; E-1) targeted the N-terminus (AA 2-29 ...
• Human skeletal muscle organoids model fetal myogenesis and sustain uncommitted PAX7 myogenic progenitors

  Lampros Mavrommatis, et al., bioRxiv - Bioengineering 2023

  Quote: ... anti-MYOD1 (Santa Cruz Biotechnologies, clone 5.8A, 1:200), anti-PRDM16 (Abcam ...
• DNA-guided transcription factor cooperativity shapes face and limb mesenchyme

  Seungsoo Kim, et al., bioRxiv - Genomics 2023

  Quote: ... TCF3 (Santa Cruz Biotechnology, sc-133074, 1:50), AP2a (Cell Signaling ...
• ULK1 forms distinct oligomeric states and nanoscopic structures during autophagy initiation

  Chiranjib Banerjee, et al., bioRxiv - Biophysics 2023

  Quote: ... The following antibodies were used for western blotting: ULK1 (sc-33182) from Santa Cruz Biotech. ...
• Sustained store-operated calcium entry utilizing activated chromatin state leads to instability in iTregs

  Huiyun Lyu, et al., bioRxiv - Immunology 2023

  Quote: ... Antibody used in immunoblot including NFATc1 Antibody (7A6) and NFATc2 Antibody (4G6-G5) were from Santa Cruz, Lamin A/C (4C11) ...
• An unconventional mechanism of IL-1β secretion that requires Type I IFN in lupus monocytes

  Simone Caielli, et al., bioRxiv - Immunology 2023

  Quote: ... and validated for stable Cas9 expression by Western blot using anti-Cas9 antibody (Santa Cruz Biotech; sc-517386). Using the Neon Transfection System (Thermo Fisher) ...
• An unconventional mechanism of IL-1β secretion that requires Type I IFN in lupus monocytes

  Simone Caielli, et al., bioRxiv - Immunology 2023

  Quote: ... insulin (10 µg/mL; Santa Cruz Biotech), Human Peripheral Blood Plasma (3% ...
• An unconventional mechanism of IL-1β secretion that requires Type I IFN in lupus monocytes

  Simone Caielli, et al., bioRxiv - Immunology 2023

  Quote: ... MG132 (5 mM; Santa Cruz Biotech) was added to the culture during phase 3 ...
• An unconventional mechanism of IL-1β secretion that requires Type I IFN in lupus monocytes

  Simone Caielli, et al., bioRxiv - Immunology 2023

  Quote: ... 0.5 mg/mL of Actinomycin D (Act D; Santa Cruz Biotech) or 10 µM of BrdU (Thermo Fisher ...