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Citations for Santa Cruz :
351 - 400 of 425 citations for n4 Benzoyl 5 methyldeoxycytidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... adenovirus was added to the medium for 3 hours on day 4 of differentiation at 250 MOI in the presence of 5 μg/ml polybrene (Santa Cruz Biotechnology). Experiments were performed 3 days later ...
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bioRxiv - Cell Biology 2020Quote: ... containing 5 % nonfat dry milk for 2 h at room temperature and then incubated with anti-NaK-ATPase (1:2500; Santa Cruz Biotechnology), anti-LAMP-1 (1:2500 ...
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bioRxiv - Immunology 2020Quote: ... m6 A-modified RNA was eluted twice in 100 mL of MeRIP buffer containing 5 mM m6 A salt (Santa Cruz Biotechnology) for 30 min at 4C with rotation ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked in 5% milk-PBST and incubated with 1% milk-PBST containing rabbit anti-Grb7 (C-20, Santa Cruz sc606), anti-β-galactosidase (Invitrogen A11132) ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked in 5% non-fat dry milk in PBST for 1 hr and incubated in SMARCAL1 (1:500; sc-376377, Santa Cruz Biotechnology) or tubulin (1:10,000 ...
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bioRxiv - Biochemistry 2024Quote: ... 30 min, 25 °C) and subsequently labelled by incubating (90 min, 25 °C) with 5-fold molar excess of BADAN (Santa Cruz Biotechnology). The labelling reaction was quenched by addition of 10 mM DTT ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies were added overnight at 4C in 5% milk powder or 10% roti block (Roth) – 1:1000 Ub antibody P4D4 (Santa Cruz Biotechnology), 1:3000 Hip1 (Proteintech 22231-1AP) ...
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bioRxiv - Immunology 2024Quote: ... Precleared extracts were incubated with 40 μL of 50% protein A/G agarose and 5 of normal rabbit IgG (Santa Cruz Biotech), H3K27ac (Active Motif ...
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bioRxiv - Immunology 2024Quote: ... Primary antibody solution was prepared with 5% (w/v) skimmed milk in TBST with anti-Parp14 mouse monoclonal antibody (1:500, sc377150, Santa Cruz Biotechnology) with enough volume for two membranes (epitope competition vs control) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Blots were then incubated with primary antibody in a 1:1000 dilution with 5% BSA in TBST overnight at 4°C: tyrosine phosphorylation using phosphotyrosine mouse monoclonal Ig pY99 (Santa Cruz Biotechnologies); mCherry expression using anti-mCherry rabbit polyclonal Ig (Rockland Inc.) ...
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bioRxiv - Bioengineering 2023Quote: ... Alomone Labs) or 5 µM of antibodies for targeting neurons with dopamine receptor 2 (D2R) (anti-D2DR, sc-5303, Santa Cruz Biotechnology) or without cell type specificity (rabbit anti-human IgG ...
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bioRxiv - Biochemistry 2024Quote: ... cells were given fresh medium containing 5 mM [13C5]-4-HV (synthesized by WuXi) or 10 mM unlabeled 4-HV sodium salt (Santa Cruz Biotechnology). Cells were cultured with 4-HV for 6 hours prior to harvest ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25; ETV6, Santa Cruz, # sc-166835x ...
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bioRxiv - Biochemistry 2024Quote: ... The membrane pellet was then solubilised in lysis buffer using a tight-fit dounce homogeniser before the addition of 5% Elugent (Santa Cruz Biotechnology) and incubated at room temperature with gentle rocking for 20 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were blocked in 5% milk and probed with primary antibodies (HA-BioLegend Clone HA.11, H3-Abcam, CDH2-Sigma-Aldrich, TWIST1-Santa Cruz, Vimentin-Cell Signaling Technology ...
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bioRxiv - Cell Biology 2024Quote: ... The lentivirus was then collected and used to infect the primary T cells/Dynabeads mixture in the presence of 5 μg/ml polybrene (Santa cruz biotechnology) by plate centrifugation (800 g ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked for 1 h (5% skim milk in TTBS) prior to overnight incubation at 4 °C with primary antibodies against p21 (1:200; Santa Cruz Biotechnology) and β-actin (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were permeabilized with 0.5% Triton-X 100 and blocked with 1% BSAprior to incubation with primary and secondary antibody (1:100) (Nox1 (Santa Cruz, sc-5821), (LRRC8D (Santa Cruz ...
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bioRxiv - Cancer Biology 2020Quote: ... n=10) and were treated with 5 mg/kg isotype specific IgG (IgG1; West Lebanon, New Hampshire) or mAb (Santa Cruz, Dallas, TX) 3 times per week ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then incubated overnight with primary antibodies in 0.3% Triton-X and 5% goat serum in PBS at the following dilutions: mouse anti-PAX6 (sc81649 Santa Cruz Biotechnology, 1:50), rabbit anti-FOXG1 (ab18259 Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells transfected with the fLuc RNAs were stained with 5 μL of anti-Luciferase antibody (C-12) PE: sc-74548 PE (Santa Cruz Biotechnology, US) while the MDR1 replicons were stained with 5 μL of PE anti-human CD243 (ABCB1 ...
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bioRxiv - Physiology 2022Quote: ... then probed with primary antibodies in 5% bovine serum albumin (BSA) in TBST overnight at 4°C with the following concentrations: Zmiz1(1:1000, Santa Cruz sc-367825), CD81 (dilution 1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was blocked using either 5% BSA (Bovine Serum Albumin) in Tris buffer Saline containing 0.1% Tween (TBST) for phosphorylated proteins or 5% Blotto (Santa Cruz Biotechnology, sc-2325) for 60 min ...
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bioRxiv - Physiology 2021Quote: ... Deparaffinized sections were blocked in 5% normal goat serum followed by overnight incubation with primary antibodies at 4°C: anti-BACE2 antibody (Santa Cruz; 1:1000), anti-insulin (Invitrogen ...
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: ... which were diluted to 1:100 in 5% BSA and 0.5% Triton X-100 in PBS: anti-BECN1 (#3738; Cell Signaling Technology and sc-48341; Santa Cruz Biotechnology), anti-PLIN1 (#9349 ...
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bioRxiv - Cancer Biology 2024Quote: ... The blots were blocked for 30 min with TBST-5% milk and then probed overnight with primary antibodies against hnRNP U (1:1000, Santa Cruz sc-32315), β-Actin (1:2000 ...
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bioRxiv - Genomics 2022Quote: ... Western Blot was blocked using 5% ELK and incubated overnight with primary antibody mouse monoclonal anti-UHRF1 (Santa Cruz, SC-373750, 1:250) or rabbit polyclonal anti-DNMT1 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Fibroblasts were incubated with primary antibody solution in 5% BSA overnight at 4°C in a moisture chamber: anti-ALIX (1:200; Santa Cruz, sc-53540), anti-Amyloid-β (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... Fibroblasts were incubated with primary antibody solution in 5% BSA overnight at 4°C in a moisture chamber: anti-ASC/PYCARD (1:100; Santa Cruz, sc-514414), anti-GAP-43 (1:300 ...
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bioRxiv - Cell Biology 2024Quote: Western blots were performed as described previously (Kuriyama and Mayor, 2008) using the following antibodies: anti Gαi2 (Santa Cruz, L-5; 1:1000); anti GAPDH (Novus Biologicals ...
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bioRxiv - Cell Biology 2024Quote: ... Membrane was blocked for one hour in 5% milk or TBS-T and incubated at RT with anti-Runx1 (Santa Cruz SC-365644X), anti-ELF1 (Santa Cruz SC-133096X ...
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bioRxiv - Cancer Biology 2024Quote: ... blocking for 30 minutes in 2,5% bovine serum albumin (BSA) and incubation with anti-GFP conjugated with Alexa Fluor™ 488 (1:300, sc-9996, Santa Cruz Biotechnology) for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... Next the membranes were washed for 5 min X 4 times with PBST and incubated with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology, SantaCruz, CA) for 1 hour ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were stained overnight with the following primary antibodies diluted 1:100 in 5% BSA in PBS at 4C: anti-MUC2 (Santa Cruz Biotechnology sc-15334), anti-ZO-1 (Santa Cruz Biotechnology sc-33725) ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (1/500 dilution in 5% nonfat free milk in TBST buffer; Santa Cruz Biotechnology, Dallas, TX) at room temperature for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked overnight with 5% BSA in PBST at 4 °C and then incubated with 1:50 RAD51 primary antibody (sc-8349, Santa Cruz or ab63801, Abcam) in 2.5% BSA in PBST for 1 hour at 37 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Membranes were blocked and probed in TBST-5% non-fat milk using anti-GFP (HRP-conjugated B-2, sc-9996 HRP, Santa Cruz, 1:5000 dilution) or anti-HA (HRP-conjugated ...
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bioRxiv - Cell Biology 2022Quote: ... blocked in 5% (v/v) goat serum in PBS and incubated with an antibody recognising the extracellular domain of EGFR (Santa Cruz, Cat# sc-101). This was followed by permeabilization as described above and incubation with an antibody recognising the C-terminus of EGFR (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then washed 3 × 5 min in TBS-T and incubated with 1:5000 goat anti-rabbit HRP secondary antibody (Santa Cruz Biotechnology, sc-2004) in 5% milk in TBS-T for 1 h followed by 3 × 5 min washes in TBS-T ...
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PVT1, a YAP1 dependent stress responsive lncRNA drives ovarian cancer metastasis and chemoresistancebioRxiv - Cancer Biology 2022Quote: ... All cell lines were grown at 37°C in a humidified incubator containing 5% CO2 and Antibodies: monoclonal mouse anti-YAP1 (Santa Cruz Biotechnology, sc-101199), Alexa Fluor 488 goat anti-mouse IgG ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was incubated with the following primary antibodies in TBST + 5% BSA at 4°C overnight: rabbit polyclonal anti-MEK-3 (1:500, Santa Cruz Biotechnology, #sc-961), rabbit polyclonal anti-p38 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated for 16 hours at 37°C in 5% CO2 then fixed in 4% paraformaldehyde in PBS pH 7.4 (Santa Cruz, Cat. No. sc-281692) with 10 μg/ml of Hoechst (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with a rabbit polyclonal anti-VP1 antibody (Ma, et al., 2020) or anti-guanylate binding protein (GBP) 1–5 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing ...
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bioRxiv - Genetics 2023Quote: After permeabilization and intercalator or enzymatic treatment the samples were incubated with 500 μl 5% (m/v) Blotto Non-Fat Dry Milk (Santa Cruz Biotechnology Inc., Santa Cruz, California, USA) in 1×PBS/EDTA for 30 minutes on ice to decrease nonspecific binding of the antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... were used to produce HCT116 H2B-GFP ABAT knock down cells at a multiplicity of infection (MOI) of 5 and 2 mg/mL of puromycin (Santa Cruz Biotechnology, #sc-108071) was used to select for knocked down cells ...
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bioRxiv - Cell Biology 2023Quote: After salt or intercalator treatment the samples were incubated with 500 μl 5% (m/v) Blotto Non-Fat Dry Milk (Santa Cruz Biotechnology Inc., Santa Cruz, California, USA) in PBS/EDTA for 30 minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... The retinas were fixed in 4% PFA for 45 minutes at room temperature and then incubated in 5% normal donkey serum (Jackson Immuno, C840D36) in 1X phosphate buffer saline (PBS) with azide (Santa Cruz Biotechnology, SC-296028) containing 0.5% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... which was probed over-night at 4°C with specific antibodies diluted in 1-3% skimmed milk-PBS-0.1% Tween solution: TNFR1 (H-5, Santa Cruz Biotechnologies, DBA, Milan, Italy), STAT3 (124H6 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then centrifuged for 5 min at 340 x g and resuspended with 200μL of 4% PFA in PBS (Santa Cruz Biotech, cat# sc-281692). All samples were acquired with a MACSQuant Analyzer 10 flow cytometer and analyzed with FloJo software version 10.4.0 (Tree Star) ...
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bioRxiv - Immunology 2024Quote: ... Membranes were blocked in 5% dried milk/TBS-T buffer and incubated over night with anti-mouse TREX1 antibody (clone C-11, Santa Cruz Biotechnology, Dallas, TX) and and anti-β-tubulin polyclonal antibody (Cell Signaling Technology ...