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• A novel iPSC model reveals selective vulnerability of neurons in Multiple Sulfatase Deficiency

  Vi Pham, et al., bioRxiv - Cell Biology 2023

  Quote: ... IgM isotype control (1:100, Santa Cruz, sc-3881). Cells were subsequently washed with saponin buffer or FACS buffer and incubated for 1 hr with secondary antibodies ...
• A novel iPSC model reveals selective vulnerability of neurons in Multiple Sulfatase Deficiency

  Vi Pham, et al., bioRxiv - Cell Biology 2023

  Quote: ... 50 µg of protein extract was incubated at 37°C for 1 hr with the artificial p-nitrocatecholsulfate substrate (Santa Cruz Biotechnology, sc-238927A) and either sodium pyrophosphate (ARSA ...
• GRAMD2⁺ alveolar type I cell plasticity facilitates cell state transitions in organoid culture

  Hua Shen, et al., bioRxiv - Cell Biology 2023

  Quote: ... Bound primary Ab was visualized using secondary Ab conjugated to horseradish peroxidase (#sc-2030,1:2000, Santa Cruz Biotechnology, Inc, Dallas, TX) and chemiluminescent substrate (ECL Plus ...
• Liver regeneration by a population of midzone-located mesenchymal-hepatocyte hybrid cells

  Guo Yu, et al., bioRxiv - Cell Biology 2023

  Quote: ... β-Catenin (1:1000, Santa Cruz Biotechnology, 7199), Hes1 (1:1000 ...
• TFEB degradation is regulated by an IKK/β-TrCP2 phosphorylation-ubiquitination cascade

  Yan Xiong, et al., bioRxiv - Cell Biology 2023

  Quote: ... antibodies to TrCP1 (C-6) (Cat# sc-390629) (1:1000) and STUB1 (Cat# sc- 133066) (1:1000) were from Santa Cruz; antibodies to V5 (Cat# MCA1360GA ...
• Unlocking cellular plasticity: Enhancing human iPSC reprogramming through bromodomain inhibition and extracellular matrix gene expression regulation

  Jun Yang, et al., bioRxiv - Cell Biology 2023

  Quote: ... OCT4 (Santa Cruz, sc-5279), SOX2 (Chemicon AB5603) ...
• YY1 binding is a gene-intrinsic barrier to Xist-mediated gene silencing

  Joseph S Bowness, et al., bioRxiv - Genomics 2023

  Quote: ... Blots were incubated with primary anti-YY1 antibody (Santa Cruz, sc-7431) or loading control anti-TBP antibody (Abcam ...
• The Hippo kinase LATS1 controls CTCF chromatin occupancy and the hormonal response of three-dimensionally grown breast cancer cells

  Julieta Ramirez, et al., bioRxiv - Genomics 2023

  Quote: ... ERα (H20, Santa Cruz); PR (H190 ...
• The Hippo kinase LATS1 controls CTCF chromatin occupancy and the hormonal response of three-dimensionally grown breast cancer cells

  Julieta Ramirez, et al., bioRxiv - Genomics 2023

  Quote: ... PR (H190, Santa Cruz); FAK (PTK2 ...
• The Hippo kinase LATS1 controls CTCF chromatin occupancy and the hormonal response of three-dimensionally grown breast cancer cells

  Julieta Ramirez, et al., bioRxiv - Genomics 2023

  Quote: ... and YAP (sc-101199, Santa Cruz).
• Nuclear dualism without extensive DNA elimination in the ciliate Loxodes magnus

  Brandon K. B. Seah, et al., bioRxiv - Genomics 2023

  Quote: ... and anti-histone H4 from Santa Cruz (sc-25260) (raised against human histone H4).
• A conserved role of hnRNPL in regulating alternative splicing of transcriptional regulators necessary for B cell activation

  Poorani Ganesh Subramani, et al., bioRxiv - Immunology 2023

  Quote: ... or control anti-IgG antibody (sc-2025, Santa Cruz Biotechnology) coupled with Dynabeads beads coated with Protein G (Life Technologies) ...
• Proximal protein landscapes of the type I interferon signaling cascade reveal negative regulation by the E3 ubiquitin ligase PJA2

  Samira Schiefer, Benjamin G. Hale, bioRxiv - Immunology 2023

  Quote: ... and incubated with the appropriate primary antibody: β-actin (Santa Cruz, sc-47778), actin (Sigma-Aldrich ...
• Proximal protein landscapes of the type I interferon signaling cascade reveal negative regulation by the E3 ubiquitin ligase PJA2

  Samira Schiefer, Benjamin G. Hale, bioRxiv - Immunology 2023

  Quote: ... STAT2 (Santa Cruz, sc-1668), phospho-Y690-STAT2 (Cell Signaling Technology ...
• Proximal protein landscapes of the type I interferon signaling cascade reveal negative regulation by the E3 ubiquitin ligase PJA2

  Samira Schiefer, Benjamin G. Hale, bioRxiv - Immunology 2023

  Quote: ... STAT1 (Santa Cruz, sc-417), phospho-Y701-STAT1 (Cell Signaling Technology ...
• Initiation of a ZAKα-dependent Ribotoxic Stress Response by the Innate Immunity Endoribonuclease RNase L

  Jiajia Xi, et al., bioRxiv - Immunology 2023

  Quote: ... The small molecule inhibitors used in this study were JNK inhibitor (SP600125, Santa Cruz, #sc-200635); p38a inhibitor (SB203580 ...
• Genome folding principles revealed in condensin-depleted mitotic chromosomes

  Han Zhao, et al., bioRxiv - Genomics 2023

  Quote: ... PMSF and 50μl of A/G agarose beads (Santa Cruz Cat#sc-2003). Preclear of chromatin was performed at 4℃ for 8h with rotation ...
• Keratin 6a attenuates Toll-like receptor-triggered proinflammatory response in corneal epithelial cells by suppressing ELKS/IKKε-dependent activation of NF-κB

  Jonathan K. Chan, et al., bioRxiv - Immunology 2023

  Quote: ... 1 mg of hTCEpi cell lysate in cell lysis buffer was precleared with 25 μl of protein A/G plus agarose (SC-2003; Santa Cruz Biotechnology) for 2 hours at 4°C with rotation before the agarose was removed by centrifugation at 2,000 rcf for 10 min ...
• Noncoding function of super enhancer derived mRNA in modulating neighboring gene expression and TAD interaction

  Bingning Xie, Ann Dean, bioRxiv - Genomics 2023

  Quote: ... CPOX (Santa Cruz Biotechnology, sc-393388), a-Tubulin (Santa Cruz Biotechnology ...
• Noncoding function of super enhancer derived mRNA in modulating neighboring gene expression and TAD interaction

  Bingning Xie, Ann Dean, bioRxiv - Genomics 2023

  Quote: ... p300 antibody (Santa Cruz Biotechnology, sc-48343). See Supplementary Data for primers.
• Noncoding function of super enhancer derived mRNA in modulating neighboring gene expression and TAD interaction

  Bingning Xie, Ann Dean, bioRxiv - Genomics 2023

  Quote: ... a-Tubulin (Santa Cruz Biotechnology, sc-5286).
• Scarring hair follicle destruction is driven by the collapse of EGFR-protected JAK-STAT1-sensitive stem cell immune privilege

  Karoline Strobl, et al., bioRxiv - Immunology 2023

  Quote: ... 80% confluent cells were treated with Erlotinib (10 nM; Santa Cruz Biotechnology) for 24 hours ...
• Structural Host-Virus Interactome Profiling of Intact Infected Cells

  Boris Bogdanow, et al., bioRxiv - Microbiology 2023

  Quote: ... anti-UL48 (mouse clone 1-21, Santa Cruz Biotechnology, sc-7545).
• Interconversion of tripartite and bipartite systems for site-specific O-sialylation of flagellin in Gram-negative and Gram-positive bacteria

  Jovelyn Unay, Nicolas Kint, Patrick Viollier, bioRxiv - Microbiology 2023

  Quote: ... OctA-Probe monoclonal antibody (at dilution 1:1000; Santa Cruz Biotechnology) or anti DYKDDDK tag monoclonal antibody (at dilution 1:1000 ...
• Lipin-1 restrains macrophage lipid synthesis to promote inflammation resolution

  Temitayo T. Bamgbose, et al., bioRxiv - Immunology 2023

  Quote: ... Membranes were washed trice with 1x TBST before 1 min incubation with ImmunoCruz Western blotting luminol reagent (Santa Cruz, sc-2048). The Amersham Imager 680 (GE Healthcare Bio-Sciences ...
• ABIN1 is a negative regulator of effector functions in cytotoxic T cells

  Sarka Janusova, et al., bioRxiv - Immunology 2023

  Quote: ... anti-A20 (clone A-12, Santa Cruz #sc-166692), anti-β-Actin (clone AC-15 ...
• NLRP3 Cys126 palmitoylation by ZDHHC7 Promotes Inflammasome Activation

  Tao Yu, et al., bioRxiv - Immunology 2023

  Quote: ... cells were washed with cold PBS and fixed with 4% paraformaldehyde solution (4% PFA, Santa Cruz) at 4 ℃ for 15 mins ...
• Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter

  Marco Di Gioia, et al., bioRxiv - Immunology 2023

  Quote: ... mouse anti-CD68 (1:100, Santa Cruz Biotechnology, Cat# sc-20060) and rat anti-Ly6G/Ly6C (1:50 ...
• Pyroptosis leads to loss of centrosomal integrity in macrophages

  Siyi Bai, et al., bioRxiv - Immunology 2023

  Quote: ... anti-ninein (mouse monoclonal, Santa Cruz, sc-376420), Anti-NLRP3 (mouse monoclonal ...
• RORγ bridges cancer-driven lipid dysmetabolism and myeloid immunosuppression

  Augusto Bleve, et al., bioRxiv - Immunology 2023

  Quote: ... goat anti-mouse actin (Santa Cruz); (secondary ...
• Pyroptosis leads to loss of centrosomal integrity in macrophages

  Siyi Bai, et al., bioRxiv - Immunology 2023

  Quote: ... mouse anti-ninein (Santa Cruz, sc-376420) and mouse anti NLRP3 (AdipoGen Life Science ...
• Proximity labeling reveals new insights into the relationships between meiotic recombination proteins in S. cerevisiae

  Karen Voelkel-Meiman, et al., bioRxiv - Genetics 2023

  Quote: ... and goat anti-rat (Santa Cruz)) were used at 1∶10,000 in 1x PBST for 1 hour at room temperature ...
• Keratin 6a attenuates Toll-like receptor-triggered proinflammatory response in corneal epithelial cells by suppressing ELKS/IKKε-dependent activation of NF-κB

  Jonathan K. Chan, et al., bioRxiv - Immunology 2023

  Quote: ... Mouse monoclonal antibodies included anti-K6a antibody (clone B7; Santa Cruz Biotechnology), anti-RelA antibody (clone A21012A ...
• An “alert state” ribosome population acts as a master regulator of cytokine-mediated processes

  Anna Dopler, et al., bioRxiv - Immunology 2023

  Quote: ... HSP 90α/β (Santa Cruz Animal Health sc-13119, 1:1000), RPL28 (Abcam ab138125 ...
• Transcriptional Regulation of the Alternative Sex Hormone-Binding Globulin Promoter by KLF4

  Warren M. Meyers, bioRxiv - Genetics 2023

  Quote: ... GAPDH (V-18, sc-20357) and histone H4 (Lys 8-R, sc-8660-R) from Santa Cruz Biotechnology.
• GATA1-defective immune-megakaryocytes as possible drivers of idiopathic pulmonary fibrosis

  Francesca Gobbo, et al., bioRxiv - Immunology 2023

  Quote: ... anti-TGF-β1 (cat no. sc-130348, Santa Cruz Biotechnology), anti-CXCL1 (cat no ...
• Bulky glycocalyx drives cancer invasiveness by modulating substrate-specific adhesion

  Amlan Barai, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti phospho-focal adhesion kinase (pFAK) rabbit monoclonal antibody (Cat# sc-374668, Santa Cruz Biotechnology), anti MMP2 (Cat # 436000 ...
• Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function

  Chao Chang, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse monoclonal anti-pTyr (PY20; Santa Cruz Biotechnology, #sc-508; RRID:AB_628122), mouse monoclonal anti-GAPDH (Santa Cruz Biotechnology ...
• Loss-of-function cancer-associated mutations in the EIF4G2 non-canonical translation initiation factor

  Sara Meril, et al., bioRxiv - Cancer Biology 2023

  Quote: ... add BCL2 (Santa Cruz Biotechnology Cat# sc-509 ...
• A high-content screen reveals new regulators of nuclear membrane stability

  Amanda L. Gunn, et al., bioRxiv - Cell Biology 2023

  Quote: ... LMNB1 (1:1000, cat#: sc-365214, Santa Cruz), SUN1 (1:1000 ...
• Coupling of Slack and Na_V1.6 sensitizes Slack to quinidine blockade and guides anti-seizure strategy development

  Tian Yuan, et al., bioRxiv - Neuroscience 2023

  Quote: ... followed by overnight incubation at 4 °C with primary antibody to AnkG (1:100, Santa Cruz, sc-31778), Slack (1:100 ...
• Coupling of Slack and Na_V1.6 sensitizes Slack to quinidine blockade and guides anti-seizure strategy development

  Tian Yuan, et al., bioRxiv - Neuroscience 2023

  Quote: ... rabbit IgG and mouse IgG was from Santa Cruz, and Protein G Dynabeads were from Invitrogen ...
• COMT inhibitor tolcapone represses the migration and invasion of trophoblast and results in preeclampsia-like phenotypes in mice with the modulation of EMT signaling

  Lu Chen, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... and anti-GAPDH (sc-47724) from Santa Cruz, USA.
• Coupling of Slack and Na_V1.6 sensitizes Slack to quinidine blockade and guides anti-seizure strategy development

  Tian Yuan, et al., bioRxiv - Neuroscience 2023

  Quote: Commercial antibodies used were: anti-AnkG (Santa Cruz), anti-Slack (NeuroMab) ...
• Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function

  Chao Chang, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse monoclonal anti-MYC (Santa Cruz Biotechnology, #sc-40; RRID:AB_627268), rabbit polyclonal anti-GFP (Thermo Fisher Scientific ...
• Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function

  Chao Chang, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse monoclonal anti-GAPDH (Santa Cruz Biotechnology ...
• Synergistic induction of blood-brain barrier properties

  Gergő Porkoláb, et al., bioRxiv - Bioengineering 2023

  Quote: ... rabbit anti-PECAM-1 (Santa Cruz, cat# sc-1506-R, AB_831096, 1:200), rabbit anti-β-catenin (Sigma-Aldrich ...
• Synergistic induction of blood-brain barrier properties

  Gergő Porkoláb, et al., bioRxiv - Bioengineering 2023

  Quote: ... protease inhibitor cocktail and sodium orthovanadate according to the manufacturer’s instructions (RIPA Lysis Buffer System, Santa Cruz, cat# sc-24948). In this case ...
• A General Framework to Interpret Hydrogen-Deuterium Exchange Native Mass Spectrometry of G-Quadruplex DNA

  Eric Largy, Matthieu Ranz, Valérie Gabelica, bioRxiv - Biophysics 2023

  Quote: ... Trimethylammonium acetate (TMAA) (1 M aqueous solution) was purchased from SantaCruz Biotechnology (Santa Cruz, CA, USA), and KCl (99.999% trace metal basis ...
• Deep Mutational Scanning in Disease-related Genes with Saturation Mutagenesis-Reinforced Functional Assays (SMuRF)

  Kaiyue Ma, et al., bioRxiv - Genetics 2023

  Quote: ... (DO NOT use IIH6C4 antibody from Santa Cruz, sc-73586 ...