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Citations for Santa Cruz :
201 - 250 of 5378 citations for Nuclear pore complex protein Nup93 dye Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 3 μg each) or isotype control antibodies (rabbit IgG or mouse IgG, respectively) were coupled to protein A/G agarose beads (Santa Cruz) in coupling buffer (0.01M sodium phosphate ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg protein extract was used for immunoprecipitation and incubated with protein G beads and 4 μg mouse anti-GFP antibody (B-2 #sc-9996, Santa Cruz). Western blotting (Fig ...
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bioRxiv - Developmental Biology 2020Quote: ... The co-IP was perform by incubating extract with protein A or G Dynabeads conjugated with antibodies against PRDM9 (custom-made) or EWSR1 (sc-6532, Santa Cruz) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were transferred into new tubes after centrifugation for 10 min at 14,000g and further incubated with the indicated antibodies for 3 hours at 4 □ followed by the addition of protein A/G beads (Santa Cruz), or with Anti-Flag magnetic beads (Bimake) ...
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bioRxiv - Plant Biology 2020Quote: ... TPR2-myc protein expression was verified by western blot using c-Myc antibody sc-789 (Santa Cruz Biotechnology, Dallas, TX, USA).
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by SDS-PAGE and immunoblotting was performed as described above with anti-BAP1 antibody (Santa Cruz: sc-28383).
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed under conditions in which only covalent interactions were preserved with anti-GFP (mouse) antibody overnight at 4°C following which Protein A agarose beads (Santa Cruz) were used to precipitate the antibody bound complex.
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bioRxiv - Cancer Biology 2022Quote: ... One mg of total protein of each sample were immunoprecipitated with 50 ul of p53 antibody (D0-1, sc-126, Santa Cruz) and incubated overnight on rotating wheel at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the detection of CDK4 proteins via a quick WB procedure using the sc-601 primary antibody from Santa Cruz Biotech ...
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bioRxiv - Cell Biology 2021Quote: ... INSR was immunoprecipitated by incubating 500 μg of protein lysate with 2 μg INSR antibodies (Rabbit pAb, Cat. #sc-711, Santa Cruz) at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µl DynabeadsTM Protein G beads were first coated with 1 µg mouse anti-MYC antibody (sc-40, Santa Cruz Biotechnology) or normal mouse IgG1 (sc-3877 ...
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bioRxiv - Molecular Biology 2024Quote: ... blots were stripped and re-probed with a polyclonal antibody directed against the immunoprecipitated GRK2 protein (C15, Santa Cruz Biotechnology Inc). Blots were developed using a chemiluminescent method (ECL ...
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bioRxiv - Immunology 2023Quote: ... supernatant was incubated with anti-NLRP3 or anti-ZDHHC7 antibody as indicated and Protein A/G conjugated agarose beads (Santa Cruz) to immunoprecipitate endogenous NLRP3 or ZDHHC7 proteins ...
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bioRxiv - Microbiology 2022Quote: ... For co-localization studies employing confocal microscopy the following antibody combinations were used: mouse anti-non-structural protein 1 (Santa Cruz)/goat anti-mouse Alexa568 (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated for 48 h at 4 °C with a goat anti-glial fibrillary acidic protein (GFAP) antibody (1:300; Santa Cruz) or a rabbit anti-ionized calcium-binding adapter molecule/allograft inflammatory factor 1 (IBA1/AIF-1 ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were cleared by centrifugation at 13000rpm for 15min at 4°C and extracts of equal amount of total protein were used to immunoprecipitated p53 using 2 μg of antibody (DO-1, Santa Cruz) and 20 μL of protein A magnetic beads (prewashed with lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Lysis supernatants were migrated in SDS-gels and used for an immunoblot to detect P53 protein with p53 antibody (Pab 1801): sc-98 (Santa Cruz).
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bioRxiv - Genetics 2024Quote: ... Protein extracts from transfected cells were analyzed by western blotting using commercial primary antibodies (Sigma anti-FLAG M2 F1804, Santa Cruz anti-GFP SC9996 ...
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bioRxiv - Cell Biology 2022Quote: ... anti-nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor α (IκBα) (#J1512, Santa Cruz Biotechnology, Dallas, TX, USA, 1:500), anti-phospho-signal transducer and activator of transcription 1 (P-STAT1 ...
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bioRxiv - Neuroscience 2021Quote: ... samples containing same protein amount were subjected to immunoprecipitation with rabbit polyclonal antibody against PDE4B (H-56) (#sc-25812, Santa Cruz Biotechnology) coupled to GammaBind Plus Sepharose beads (# 17-0886-02 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated overnight with combinations of the following primary antibodies: rabbit anti-protein kinase C (anti-PKC) clone C20 (1:1000; Santa Cruz Biotechnology), rabbit anti-phospho-histone H3 (1:2500 ...
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bioRxiv - Cell Biology 2020Quote: ... Thirty micrograms of total protein were separated by SDS-PAGE and probed with primary antibodies against NFATC2 (Santa Cruz Biotechnology, sc-7296), NFATC4 (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... the total protein was detected by using specific antibodies (GRK2: Santa Cruz sc-13143; GRK3: Cell signaling technology #80362; GRK5: Santa Cruz, sc-518005 ...
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bioRxiv - Neuroscience 2022Quote: ... cell culture supernatants of cultured cerebellar neurons were incubated overnight with Reelin antibody G10 and Protein A/G beads (Santa Cruz Biotechnologies) or brain homogenates were resuspended in immunoprecipitation buffer (25mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used for Western blot of β-actin and AK2 proteins were: anti-actin (Santa Cruz Biotechnology sc-47778, 1:1000), anti-AK2 (ProteinTech 11014-1-AP ...
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bioRxiv - Plant Biology 2021Quote: ... the protein-retained beads were boiled in Laemmli buffer and immunoblotted using anti-GST and anti-MBP antibodies (Santa Cruz Biotechnology, USA).
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bioRxiv - Cancer Biology 2021Quote: ... protein lysates (500 μL of NP-40 buffer containing 200 μg of protein) were incubated with 10 μg of primary antibody Neu (F-11) agarose conjugate (sc-7301; Santa Cruz Biotechnology) overnight at 4°C with gentle rotation ...
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bioRxiv - Bioengineering 2020Quote: Tissue sections were incubated overnight with polyclonal rabbit antibodies raised against c-fos protein (1:1000; Santa Cruz Biotechnology Inc.; sc-253), GFAP (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... CBLB and GAPDH in the protein blots were probed with anti-CBLB- and anti-GAPDH-antibodies (SC-8006 and SC-166545, respectively; Santa Cruz Biotechnology). Flow Cytometry analysis ...
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bioRxiv - Immunology 2023Quote: ... Clarified lysates were incubated with the appropriate antibodies for 2h at 4°C and subsequently with protein A/G agarose beads (Santa Cruz Biotechnology) for 1h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated at 30 °C for 30 minutes or 1 hour before separation by SDS–PAGE followed by immunoblot analysis for MBP-substrate proteins (Anti-MBP-probe Antibody (R3.2) (Santa Cruz sc-53366)).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Aliquots of lysates containing 100 μg of protein were used for each immunoprecipitation reaction with indicated antibodies followed by adsorption to protein A/G PLUS-agarose beads (Cat#: sc-2003, Santa Cruz Biotechnology). DNA-protein cross-link was reversed by heating the samples to 65 °C overnight ...
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bioRxiv - Immunology 2022Quote: ... WCL were incubated with GLUT3 antibody overnight and then with Protein A/G PLUS-Agarose bead slurry (Santa Cruz Biotechnology, sc-2003) for 3h ...
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bioRxiv - Neuroscience 2023Quote: ... Immunodetection of ubiquitinated proteins was accomplished by incubating the membrane overnight with 1:1000 diluted monoclonal ubiquitin antibody P4D1 (sc-8017; Santa Cruz Biotechnology) and exposing the membranes to peroxidase-conjugated anti-rabbit secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were incubated overnight with RIP immunoprecipitation buffer containing magnetic Protein G beads conjugated with human SFPQ/PSF antibody (sc-271796, Santa Cruz, Cliniscience) or negative control mouse IgG (5415S ...
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bioRxiv - Molecular Biology 2024Quote: ... the total cell lysates were incubated with anti-phosphotyrosine antibody (PY99) followed by Protein A/G PLUS Agarose (both from Santa Cruz Biotechnology). The total cell lysates or the anti-phosphotyrosine immunoprecipitates were subjected to western blotting ...
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bioRxiv - Microbiology 2024Quote: ... and the remaining three aliquots were immunoprecipitated overnight at 4 °C with the indicated antibody mixed with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... the extracted lysates were first pre-cleared with protein G-magnetic beads at 4 °C for 2 h followed by incubation with HOXB13 antibody (2 μg/sample, Santa Cruz, sc-28333) overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein extracts were incubated with each antibody with rotation for 2 hr and then added to protein A/G agarose beads (Santa Cruz, sc-2003) for an additional 1 hr ...
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bioRxiv - Cancer Biology 2020Quote: BCR-ABL protein expression was assessed by SDS-PAGE and western blotting using a mouse anti-ABL antibody (1:1,000, Santa Cruz Biotechnology, CA, USA). Horseradish peroxidase-conjugated α-mouse antibody (1:10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 20 h at 4 °C in the blocking buffer with goat IgG antibodies specific for the human AIF protein (Santa Cruz Biotechnology, USA) diluted 1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... Clones were screened for gene deletion by PCR as well as LARP1 mRNA and protein depletion determined by qPCR and western blot analysis with a monoclonal LARP1 antibody (Santa Cruz sc-515873). Lesions at the locus of cutting were identified by PCR using genomic DNA to amplify the locus followed by Sanger sequencing and Synthego ICE analysis.
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bioRxiv - Developmental Biology 2022Quote: Western blotting was performed using 4–20% Mini-PROTEAN® TGX™ precast protein gels with the following antibodies anti-NFATc1 (1:1000, clone 7A6, Santa Cruz) and anti-GAPDH (1:2000 ...
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bioRxiv - Genetics 2020Quote: ... Section of the membrane with 75-50 kDa proteins was incubated with rat monoclonal antibodies against yeast α-tubulin (sc-53030, Santa Cruz Biotechnology) at 1:2,000 – 1:4,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase ...
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bioRxiv - Microbiology 2023Quote: ... To determine interaction between BicDR1 and the viral capsid protein p24 cells were permeabilized and blocked using 3% donkey serum followed by incubation with primary antibodies targeting p24 (Santa Cruz sc-69728) and BicDR1 (Atlas Antibodies HPA041309) ...
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bioRxiv - Neuroscience 2024Quote: ... RGCs were labeled by incubation with a primary goat antibody raised against Brn3a (brain-specific homeobox/POU domain protein 3A, Santa Cruz Biotechnology, Santa Cruz, CA), 1:100 in PBS with 2% bovine serum albumin ...
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bioRxiv - Molecular Biology 2024Quote: ... at 1:10,000 dilution and the lower portion was incubated with a mouse monoclonal antibody against histone H3 protein (Santa Cruz Biotechnologies #sc-517576) at 1:5,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... supernatant from immunoprecipitation experiments was incubated with primary antibody overnight at 4°C followed by incubation with 20 µL of Protein A/G Plus-agarose beads (Santa Cruz Biotechnology, Inc.) for 2 h at 4°C the following day ...