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Citations for Santa Cruz :
201 - 250 of 5356 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Immunodetection of ubiquitinated proteins was accomplished by incubating the membrane overnight with 1:1000 diluted monoclonal ubiquitin antibody P4D1 (sc-8017; Santa Cruz Biotechnology) and exposing the membranes to peroxidase-conjugated anti-rabbit secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were incubated overnight with RIP immunoprecipitation buffer containing magnetic Protein G beads conjugated with human SFPQ/PSF antibody (sc-271796, Santa Cruz, Cliniscience) or negative control mouse IgG (5415S ...
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bioRxiv - Molecular Biology 2024Quote: ... the total cell lysates were incubated with anti-phosphotyrosine antibody (PY99) followed by Protein A/G PLUS Agarose (both from Santa Cruz Biotechnology). The total cell lysates or the anti-phosphotyrosine immunoprecipitates were subjected to western blotting ...
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bioRxiv - Microbiology 2024Quote: ... and the remaining three aliquots were immunoprecipitated overnight at 4 °C with the indicated antibody mixed with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... the extracted lysates were first pre-cleared with protein G-magnetic beads at 4 °C for 2 h followed by incubation with HOXB13 antibody (2 μg/sample, Santa Cruz, sc-28333) overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein extracts were incubated with each antibody with rotation for 2 hr and then added to protein A/G agarose beads (Santa Cruz, sc-2003) for an additional 1 hr ...
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bioRxiv - Cancer Biology 2020Quote: BCR-ABL protein expression was assessed by SDS-PAGE and western blotting using a mouse anti-ABL antibody (1:1,000, Santa Cruz Biotechnology, CA, USA). Horseradish peroxidase-conjugated α-mouse antibody (1:10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 20 h at 4 °C in the blocking buffer with goat IgG antibodies specific for the human AIF protein (Santa Cruz Biotechnology, USA) diluted 1:200 ...
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bioRxiv - Molecular Biology 2022Quote: ... Clones were screened for gene deletion by PCR as well as LARP1 mRNA and protein depletion determined by qPCR and western blot analysis with a monoclonal LARP1 antibody (Santa Cruz sc-515873). Lesions at the locus of cutting were identified by PCR using genomic DNA to amplify the locus followed by Sanger sequencing and Synthego ICE analysis.
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bioRxiv - Developmental Biology 2022Quote: Western blotting was performed using 4–20% Mini-PROTEAN® TGX™ precast protein gels with the following antibodies anti-NFATc1 (1:1000, clone 7A6, Santa Cruz) and anti-GAPDH (1:2000 ...
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bioRxiv - Genetics 2020Quote: ... Section of the membrane with 75-50 kDa proteins was incubated with rat monoclonal antibodies against yeast α-tubulin (sc-53030, Santa Cruz Biotechnology) at 1:2,000 – 1:4,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase ...
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bioRxiv - Microbiology 2023Quote: ... To determine interaction between BicDR1 and the viral capsid protein p24 cells were permeabilized and blocked using 3% donkey serum followed by incubation with primary antibodies targeting p24 (Santa Cruz sc-69728) and BicDR1 (Atlas Antibodies HPA041309) ...
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bioRxiv - Neuroscience 2024Quote: ... RGCs were labeled by incubation with a primary goat antibody raised against Brn3a (brain-specific homeobox/POU domain protein 3A, Santa Cruz Biotechnology, Santa Cruz, CA), 1:100 in PBS with 2% bovine serum albumin ...
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bioRxiv - Molecular Biology 2024Quote: ... at 1:10,000 dilution and the lower portion was incubated with a mouse monoclonal antibody against histone H3 protein (Santa Cruz Biotechnologies #sc-517576) at 1:5,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... supernatant from immunoprecipitation experiments was incubated with primary antibody overnight at 4°C followed by incubation with 20 µL of Protein A/G Plus-agarose beads (Santa Cruz Biotechnology, Inc.) for 2 h at 4°C the following day ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µg of protein were first separated by SDS-PAGE and then blotted with the following antibodies: MSH3 (1:500, Santa Cruz, sc-271080), huntingtin (1:2,000 ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining supernatant was subjected to immunoprecipitation using 15 μl of mouse monoclonal antibody against green fluorescent protein (catalog no. sc-9996, Santa Cruz, CA, USA). The immunoprecipitate was gently shaken overnight at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS-PAGE and expression was detected using the following antibodies: ATF3 (sc-81189, Santa Cruz Biotechnology, Dallas, TX, USA), cleaved caspase-3 (#9664 ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatants were immunoprecipitated with either Arc antibody (rabbit polyclonal, custom-made; Protein Tech) or normal rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) at 1 μg/500 μL lysate for 2 h at 4°C with gentle rocking ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatants were immunoprecipitated with either Arc antibody (rabbit polyclonal, custom-made; Protein Tech) or normal rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) at 1 μg/500 μL lysate for 2 h at 4°C with gentle rocking ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 1 mg of protein was used for Co-IP using anti-HA magnetic beads (Thermo, 88837) or Protein G magnetic beads (Thermo, 10003D) complexed with anti-Heph antibody (Santa Cruz, SC-365365) according to manufacturer’s instructions22 ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracts were incubated with 1-2 μg of anti-eIF4E (sc-271480) antibody for 16 h at 4°C before protein G-PLUS-agarose slurry (Santa Cruz sc-2002) was added for 2 h at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... brain tissue was processed for DREADDs expression validation and selected tissue was stained for antibody against striatal-enriched protein tyrosine phosphatase (STEP, mouse, dilution 1:500, Santa Cruz sc-23892).
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bioRxiv - Neuroscience 2024Quote: ... The sections underwent permeabilization with triton-x and were incubated overnight at 4 degrees with 1:500 of goat raised glial fibrillary acidic protein primary antibody (GFAP; SC-6170; Santa Cruz Biotechnologies, Santa Cruz, CA). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... Co-IP was performed using anti-PNKP (BioBharati Life Science, BB-AB0105) antibody with Protein A/G PLUS agarose beads (Santa Cruz Biotechnology, sc2003,) overnight ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 500 μg of the supernatant proteins were incubated with 2 μg of mouse monoclonal antibody raised against SNAP-25 (Santa Cruz, sc-390814) or Syntaxin1a (MilliporeSigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Separated proteins were transferred to a PVDF membrane and the membrane was incubated with antibodies to the HA epitope tag (Santa Cruz Biotechnology, sc-805) and α tubulin (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... heat shock protein 90A/B (HSP90) was detected using a rabbit polyclonal antibody (H-114, 1:2500 dilution, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were incubated with the antibodies for 1h at 4°C on a roller before 80µl Protein G PLUS-Agarose beads (Santa Cruz Biotechnology, Dallas, TX, USA) were added and samples were incubated overnight at 4°C on a roller ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with a rabbit polyclonal anti-VP1 antibody (Ma, et al., 2020) or anti-guanylate binding protein (GBP) 1–5 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins extracts (1.5 mg) of embryos expressing CG11412-myc were incubated with 1 μg of c-Myc antibody (Santa Cruz, sc-40, clone 9E10) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... the HIS-tagged AtCFI25a protein bound to GST-AtCFI59 was detected by western blot analysis using an anti-HIS antibody (G18) (sc-804; Santa Cruz Biotechnology, Dallas, USA) at 1:5000 dilution.
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein were loaded per lane and blots were probed with anti-mGFP antibodies (Santa Cruz Biotech, #sc-9996; 1:2000 dilution), anti-elongation factor 1-α (EF1A ...
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bioRxiv - Molecular Biology 2022Quote: ... The remained liquids were added with anti-NS1 or -VP2 or -IgG antibodies and incubated at 4°C for 4 h followed by addition of protein A/G PLUS-Agarose (Santa Cruz Biotech, sc-2003) and incubation overnight ...
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bioRxiv - Cell Biology 2023Quote: ... were immunoprecipitated by incubation with protein A/G magnetic beads (HY-K0202, MCE) and anti-GFP antibody (sc-9996, Santa Cruz Biotechnology; 1:50) at 4 °C overnight with gentle rocking ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Samples were incubated with primary antibody or rabbit IgG (control) for 3hr at 4°C and then with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, sc-2003) beads for 35min with head-to-tail rotation either in the absence or presence of 100 µM CCT blocking peptide SAGRRFIVSPVPE (United Biosystems) ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were incubated with primary antibody (control sample incubated with rabbit IgG primary antibody: 1:100) overnight at 4°C and then with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, sc-2003) beads for 1 hour with head-to-tail rotation ...
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bioRxiv - Cancer Biology 2024Quote: ... Immunoprecipitation was performed as previously described (3) and the bound proteins were analyzed by western blotting using the following antibodies: anti-AnxA6 (Santa Cruz Biotechnology sc-271859), anti-β-actin (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... cultured cells were homogenized in IP cell lysis buffer (Beyotime) and incubated with primary antibody and Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, sc-2003) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... After each chase period the cells were lysed in RIPA buffer and XPO1 protein was recovered from whole lysates by immunoprecipitation using mouse monoclonal XPO1 antibody (Santa Cruz Biotech, sc-5595) and subjected to SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Cell Biology 2020Quote: ... heat shock protein 90A/B (HSP90) was detected using a rabbit polyclonal antibody (H-114, 1:2500 dilution, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cancer Biology 2022Quote: Protein expression was quantified by western blot analysis using the following antibodies: anti-cyclin A (1:500 sc-751; Santa Cruz Biotechnology, Dallas, TX, USA), anti-Histone H3 (phospho Serine10 ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... 500-1000ug protein extracts were incubated under rotary agitation overnight at 4 °C with antibodies against SMARCA4 (Santa Cruz Biotechnology, sc-374197 X; 4 µg), YAP (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to PVDF membrane (Amersham 0.2 Hybond P, Cytiva) and revealed using following antibodies: mouse AGR2 (Santa Cruz Biotechnology, Clone 6C5, 1/1000), rabbit IRE1β (gift from David Ron ...
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bioRxiv - Neuroscience 2024Quote: ... RGCs were labeled by incubation with a primary goat antibody raised against Brn3a (brain-specific homeobox/POU domain protein 3A, Santa Cruz Biotechnology, Santa Cruz, CA), 1:100 in PBS with 2% bovine serum albumin ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used to probe GST or epitopes (V5, Flag, or HA) for HSV-1 proteins include mouse monoclonal GST antibody (Santa Cruz Biotechnology Cat#: sc-138) diluted at 1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were blocked with 5% milk/TBST for 30 min and incubated with primary antibodies/TBST against proteins of interest: anti-RBM12 (Santa Cruz Biotechnology, Cat. #sc-514258), anti-PKA alpha polyclonal antibody (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... Tumour lysates were subjected to SDS-PAGE followed by protein transfer and immunoblotting with primary antibodies (anti EPLIN, Novus Cat NB100-2305 and anti β-actin, Santa Cruz Cat sc-47778). Signal amplification was performed with HRP-conjugated secondary antibodies (Goat Anti Mouse IgG and Anti Rabbit IgG von Southern Biotek ...