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Citations for Santa Cruz :
2351 - 2400 of 2551 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Whole-cell protein lysates were prepared from hCO or enriched astrocytes using RIPA lysis buffer (Santa Cruz Biotechnology, sc-24948A) within HypoxyLab after 48 hr of hypoxia exposure ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF10A-derived 1E7 cells cultured on tissue culture polystyrene were induced with 1 μg/mL doxycycline (204734, Santa Cruz Biotechnology) for 24 hours before being collected with 0.05% trypsin-EDTA.
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bioRxiv - Cancer Biology 2023Quote: ... Blots were incubated with secondary antibody (anti-rabbit IgG-HRP, Cell Signaling Technology; anti-mouse IgG-HRP, Santa Cruz Biotechnology) at 1:5000 for 2 hours and developed using Pierce ECL Western Blotting Substrate or SuperSignal West Femto Substrate (Thermo Fisher Scientific).
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bioRxiv - Systems Biology 2023Quote: ... total PRAS40 (Cell Signaling Technologies #2691, diluted 1:1000, and 14-3-3 (Santa Cruz #sc-1657, diluted 1:5000). The following day membranes were washed 3 x 10 min in TBS-T and incubated for 1 h in species-appropriate fluorescent antibodies ...
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bioRxiv - Microbiology 2023Quote: ... A549 or HSAE cells were transduced with the lentiviruses in the presence of 10 µg/mL polybrene (Santa Cruz Biotechnology). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... media was removed and cells incubated with 20 mL of PBS containing 2 mM disuccinimidyl glutarate (DSG) (Santa Cruz Chemical) for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells transfected with HA-TAp73-pcDNA3 plasmid were incubated with rabbit anti-HA antibody (Santa Cruz #805, Dallas, TX, USA) diluted 1:200 in PBS for 1h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The filtered viruses were added to culture media of cells with polybrene (Santa Cruz, final concentration of 8 µg/mL). Fresh media were replaced 48 hours after infection ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated overnight at 4°C with a mouse antibody recognizing GFP (Santa Cruz, sc-9996, 1:300) and a rabbit antibody recognizing TGN46 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were knocked down by small interfering RNA (siRNA) targeting galectin-3 (Santa Cruz Biotechnology, USA ref: sc-155994-SH) according to the manufacturer’s siRNA transfection protocol.
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bioRxiv - Microbiology 2023Quote: ... were added to the cell culture at an MOI of 0.25 in the presence of polybrene (Santa Cruz, Dallas, Texas). At 24 hours post-transduction ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed and incubated with secondary antibody (mouse anti-rabbit IgG-CFL 647, [Santa Cruz Biotechnology, INC.; sc-516251]) for 2 h at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... plasmid containing puromycin resistance gene and RFP gene (sc-400013-HDR) were transfected into cells according to the manufacturer’s recommendations (Santa Cruz). Control CRISPR/Cas9 plasmid (sc-41822 ...
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bioRxiv - Microbiology 2024Quote: ... the blocked cells were washed twice with PBS and then stained with anti-mouse PSGL-1 (KPL1; Santa Cruz Biotechnology) and anti-mouse BST2 antibody (E-4 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were incubated with media enriched in viral particles and supplemented with 10 μg mL−1 polybrene (Santa Cruz Biotechnology) for 6 h ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated for 48 h after adding 5 mM (final conc.) of N-methyl-DL-aspartic acid (MDLA) (Santa Cruz), solved in water ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... lysates obtained from 15-30 million cells were immunoprecipitated with 10 μg of antibodies against either Myc (Santa Cruz, #sc-764), ER-alpha (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... the same amount of cell lysate was added to the same volume of or Mouse IgG antibody (Santa Cruz, sc-2025). Following incubation ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were pulse-labelled with 10 μM EdU for 20 minutes prior to fixation in 4% paraformaldehyde in PBS (Santa Cruz) containing 0.5% Triton X-100 for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... revealed with a Cy5-goat anti-mouse (1:1,000; Jackson) and the phenotype of IdU-IR cells using Calbindin (Calb, 1:250; Santa Cruz Biotechnologies) revealed with Alexa-Fluor 647 donkey anti-goat (1/1000 ...
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bioRxiv - Biochemistry 2021Quote: ... followed by enrichment of HLA-I positive cells through up to two serial rounds of fluorescent activated cell sorting (FACS) and isolation using a pan-HLA-I antibody (W6/32; Santa Cruz) on a FACSAria II instrument (BD Biosciences) ...
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bioRxiv - Biochemistry 2021Quote: ... in press). Antibodies used to validate Sec61 and/or EMC subunit depletions in SP cells (Fig. S2) were purchased from Santa Cruz Biotechnology (goat polyclonal anti-LMNB1 (clone M-20 ...
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bioRxiv - Biochemistry 2020Quote: ... GβL (sc-425272) and SUMO1/2/3 deletion were carried out in striatal cells using CRISPR/Cas-9 tools obtained from Santa Cruz, as described before [46 ...
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bioRxiv - Immunology 2021Quote: ... 1×108 Jurkat T cells were transduced with lentivirus at an MOI of 0.5 with 8 ug/ml polybrene (Santa Cruz Biotechnology) and spinfected at 1000x g for 1.5 hours at 32°C ...
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bioRxiv - Microbiology 2021Quote: ... The phosphorylation of EGFR in mouse oral epithelial cells was determined similarly except that total EGFR was detected with anti-EGFR antibody from Santa Cruz Biotechnology (# SC373746 ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... coli cells were isolated by chromatin immunoprecipitation (ChIP) with the specific antibodies that specifically recognize myc tag (9E10, Santa Cruz Biotechnology), and Dynabeads Pan Mouse IgG magnetic beads (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... U2OS and HEK293 ATM KO cells were generated using the ATM CRISPR/Cas9 KO and ATM HDR plasmids (sc-400192, sc-400192-HDR from Santa Cruz) according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2020Quote: ... Post-nuclear supernatant from UV exposure cells was also prepared and was subjected to Western blot analysis with antibodies to BRCA1 (1:500, Santa Cruz), GFP (1:3000 ...
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bioRxiv - Cell Biology 2020Quote: Total protein from cultured cells was homogenized in RIPA lysis buffer with a mixture of protease and phosphatase inhibitors (Santa Cruz). Protein concentration was determined using the Bradford assay (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed and preincubated with Protein A/B PLUS agarose beads (Cat# sc-2003, Santa Cruz Biotech, Dallas, TX, USA) for 2 h ...
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bioRxiv - Microbiology 2022Quote: ... where after cells were stained with NF-κB p65 antibody (1:200 dilution in 1% BSA in PBS; Santa Cruz Biotechnology) in the dark for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... glabrata cells were coated with 20% heat-inactivated or fresh serum in the presence of an anti-kininogen antibody (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2021Quote: Cell lysates and pull-down samples were subjected to SDS-PAGE and Western blots performed using antibodies against HSP90 (Santa Cruz), TRIM25 (Abcam) ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies diluted in 1% NGS blocking buffer were added to the cultured cells overnight at 4°C (Oct4, 1:100, Santa Cruz; β-III-tubulin 1:500 ...
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bioRxiv - Cell Biology 2022Quote: Drugs were added to cells at the time of Tmx induction at the following concentrations: Cytochalasin D (Santa Cruz SC-201442) (200nM in DMSO) ...
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bioRxiv - Microbiology 2022Quote: ... and probed with first antibodies (rabbit anti-phospho-IRF3, catalogue no.4947, Cell Signalling, mouse anti-NS1, catalogue no. sc-130568, Santa Cruz, rabbit anticalnexin ...
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bioRxiv - Neuroscience 2022Quote: ... FG labelled cells were visualized on 40 µm coronal slices counterstained with a 5 μg/ml propidium iodide (Santa Cruz Biotechnology) staining solution in PBS 1x for 10 min ...
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bioRxiv - Systems Biology 2019Quote: ... coli cells were isolated by chromatin immunoprecipitation (ChIP) with the specific antibodies that specifically recognize myc tag (9E10, Santa Cruz Biotechnology), and Dynabeads Pan Mouse IgG magnetic beads (Invitrogen ...
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bioRxiv - Biophysics 2019Quote: ... MA, USA), phospho Histone H2A.X (D17A3) (Cell Signalling Technology, Danvers, MA, USA) and CD86 B7-2 (GL-1) (Santa Cruz Technologies) combined with Alexa 647 and Alexa 488 conjugated secondary antibodies (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... Respective cells were incubated with anti-c-Myc antibody (9E10, mouse monoclonal, 2 mg/ml; Santa Cruz Biotechnology, Dallas, TX, USA) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... The cells were then incubated with anti-UCHL1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-25800, diluted 1:200), GFRα1 (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×105 cells were pre-incubated for 1-2 h in N2 medium without antibiotics and 1 μg/μl of polybrene (Santa Cruz) to increase transduction efficiency ...
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bioRxiv - Immunology 2019Quote: ... Endogenous ELF1 protein levels from cell lysates or mouse lung homogenates were measured by western blotting using anti-ELF1 antibody (1:5000, Santa Cruz).
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bioRxiv - Pathology 2020Quote: ... Transfected cells were selected by selection media containing 2 μg/ml puromycin dihydrochloride (cat. no. sc-108071; Santa Cruz Biotechnology, Inc.). After confirming the decrease in LOXL2 expression by reverse transcription-quantitative polymerase chain reaction (RT-qPCR ...
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bioRxiv - Pathology 2020Quote: ... HK-2 cells were treated with media containing 5 μg/ml of polybrene (cat. no. sc-134220; Santa Cruz Biotechnology, Inc.), and then LOXL2 shRNA lentiviral particles and control shRNA lentiviral particles of 1 and 2 multiplicity of infection (MOI ...
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bioRxiv - Immunology 2019Quote: Cells were analysed for ligand anchor or HLA-A*02 surface expression by flow cytometry using anti-HA-Tag antibody Alexa Fluor 647 (6E2; Cell Signalling Technology) and anti-HLA-A2 FITC antibody (BB7.2; Santa Cruz Biotechnology) respectively (BD FACSCalibur™ ...
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bioRxiv - Cell Biology 2020Quote: ... After a further centrifuge red blood cells were lysed in 1 ml of RBC lysis buffer (Santa Cruz Biotechnology sc-296258) in ice for 2.5 minutes ...
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bioRxiv - Immunology 2020Quote: ... and cells were re-suspended in 100 μl 1:500 rabbit anti-ASC (N-15)-R (Santa Cruz, sc-22514-R) PBS/3%FCS/0.1% saponin and incubated overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: Human T cells (2-5 x 106) were transfected with 3 μg of TERT KO CRISPR/Cas9 plasmid (h) (sc-400316, Santa Cruz) according to the manufacturer instructions ...
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bioRxiv - Genetics 2019Quote: ... the proteins from cotransfected cell lysates after 24 hours were precipitated with an antibody against the N-terminus of ZP1 (G-20, Santa Cruz). Then ...