Labshake search
Citations for Santa Cruz :
2351 - 2396 of 2396 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked in 5% non-fat dry milk in PBST for 1 hr and incubated in SMARCAL1 (1:500; sc-376377, Santa Cruz Biotechnology) or tubulin (1:10,000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies were added overnight at 4C in 5% milk powder or 10% roti block (Roth) – 1:1000 Ub antibody P4D4 (Santa Cruz Biotechnology), 1:3000 Hip1 (Proteintech 22231-1AP) ...
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bioRxiv - Immunology 2024Quote: ... Precleared extracts were incubated with 40 μL of 50% protein A/G agarose and 5 of normal rabbit IgG (Santa Cruz Biotech), H3K27ac (Active Motif ...
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bioRxiv - Immunology 2024Quote: ... Primary antibody solution was prepared with 5% (w/v) skimmed milk in TBST with anti-Parp14 mouse monoclonal antibody (1:500, sc377150, Santa Cruz Biotechnology) with enough volume for two membranes (epitope competition vs control) ...
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bioRxiv - Bioengineering 2023Quote: ... Alomone Labs) or 5 µM of antibodies for targeting neurons with dopamine receptor 2 (D2R) (anti-D2DR, sc-5303, Santa Cruz Biotechnology) or without cell type specificity (rabbit anti-human IgG ...
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bioRxiv - Biochemistry 2024Quote: ... The membrane pellet was then solubilised in lysis buffer using a tight-fit dounce homogeniser before the addition of 5% Elugent (Santa Cruz Biotechnology) and incubated at room temperature with gentle rocking for 20 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were blocked in 5% milk and probed with primary antibodies (HA-BioLegend Clone HA.11, H3-Abcam, CDH2-Sigma-Aldrich, TWIST1-Santa Cruz, Vimentin-Cell Signaling Technology ...
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bioRxiv - Cell Biology 2024Quote: ... The lentivirus was then collected and used to infect the primary T cells/Dynabeads mixture in the presence of 5 μg/ml polybrene (Santa cruz biotechnology) by plate centrifugation (800 g ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were lysed in RIPA buffer and immunoblotting was performed using primary antibodies: XPO1 (CRM1 rabbit polyclonal H-300; sc-5595, Santa Cruz or CRM1 mouse monoclonal (C-1): sc-74454) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were permeabilized with 0.5% Triton-X 100 and blocked with 1% BSAprior to incubation with primary and secondary antibody (1:100) (Nox1 (Santa Cruz, sc-5821), (LRRC8D (Santa Cruz ...
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bioRxiv - Cancer Biology 2020Quote: ... n=10) and were treated with 5 mg/kg isotype specific IgG (IgG1; West Lebanon, New Hampshire) or mAb (Santa Cruz, Dallas, TX) 3 times per week ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then incubated overnight with primary antibodies in 0.3% Triton-X and 5% goat serum in PBS at the following dilutions: mouse anti-PAX6 (sc81649 Santa Cruz Biotechnology, 1:50), rabbit anti-FOXG1 (ab18259 Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was blocked using either 5% BSA (Bovine Serum Albumin) in Tris buffer Saline containing 0.1% Tween (TBST) for phosphorylated proteins or 5% Blotto (Santa Cruz Biotechnology, sc-2325) for 60 min ...
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: ... which were diluted to 1:100 in 5% BSA and 0.5% Triton X-100 in PBS: anti-BECN1 (#3738; Cell Signaling Technology and sc-48341; Santa Cruz Biotechnology), anti-PLIN1 (#9349 ...
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bioRxiv - Cancer Biology 2024Quote: ... The blots were blocked for 30 min with TBST-5% milk and then probed overnight with primary antibodies against hnRNP U (1:1000, Santa Cruz sc-32315), β-Actin (1:2000 ...
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bioRxiv - Genomics 2022Quote: ... Western Blot was blocked using 5% ELK and incubated overnight with primary antibody mouse monoclonal anti-UHRF1 (Santa Cruz, SC-373750, 1:250) or rabbit polyclonal anti-DNMT1 (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: Western blots were performed as described previously (Kuriyama and Mayor, 2008) using the following antibodies: anti Gαi2 (Santa Cruz, L-5; 1:1000); anti GAPDH (Novus Biologicals ...
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bioRxiv - Cell Biology 2024Quote: ... Membrane was blocked for one hour in 5% milk or TBS-T and incubated at RT with anti-Runx1 (Santa Cruz SC-365644X), anti-ELF1 (Santa Cruz SC-133096X ...
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bioRxiv - Cancer Biology 2024Quote: ... blocking for 30 minutes in 2,5% bovine serum albumin (BSA) and incubation with anti-GFP conjugated with Alexa Fluor™ 488 (1:300, sc-9996, Santa Cruz Biotechnology) for 1 hour ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were stained overnight with the following primary antibodies diluted 1:100 in 5% BSA in PBS at 4C: anti-MUC2 (Santa Cruz Biotechnology sc-15334), anti-ZO-1 (Santa Cruz Biotechnology sc-33725) ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (1/500 dilution in 5% nonfat free milk in TBST buffer; Santa Cruz Biotechnology, Dallas, TX) at room temperature for 1 h ...
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bioRxiv - Plant Biology 2021Quote: ... Membranes were blocked and probed in TBST-5% non-fat milk using anti-GFP (HRP-conjugated B-2, sc-9996 HRP, Santa Cruz, 1:5000 dilution) or anti-HA (HRP-conjugated ...
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bioRxiv - Cell Biology 2022Quote: ... blocked in 5% (v/v) goat serum in PBS and incubated with an antibody recognising the extracellular domain of EGFR (Santa Cruz, Cat# sc-101). This was followed by permeabilization as described above and incubation with an antibody recognising the C-terminus of EGFR (Santa Cruz ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with a rabbit polyclonal anti-VP1 antibody (Ma, et al., 2020) or anti-guanylate binding protein (GBP) 1–5 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing ...
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bioRxiv - Genetics 2023Quote: After permeabilization and intercalator or enzymatic treatment the samples were incubated with 500 μl 5% (m/v) Blotto Non-Fat Dry Milk (Santa Cruz Biotechnology Inc., Santa Cruz, California, USA) in 1×PBS/EDTA for 30 minutes on ice to decrease nonspecific binding of the antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... were used to produce HCT116 H2B-GFP ABAT knock down cells at a multiplicity of infection (MOI) of 5 and 2 mg/mL of puromycin (Santa Cruz Biotechnology, #sc-108071) was used to select for knocked down cells ...
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bioRxiv - Cell Biology 2023Quote: After salt or intercalator treatment the samples were incubated with 500 μl 5% (m/v) Blotto Non-Fat Dry Milk (Santa Cruz Biotechnology Inc., Santa Cruz, California, USA) in PBS/EDTA for 30 minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... The retinas were fixed in 4% PFA for 45 minutes at room temperature and then incubated in 5% normal donkey serum (Jackson Immuno, C840D36) in 1X phosphate buffer saline (PBS) with azide (Santa Cruz Biotechnology, SC-296028) containing 0.5% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Genetics 2024Quote: ... The membranes were blocked with 5% non-fat milk and immunoblotted with primary antibodies against CFTR (769 from the UNC distribution program) or GAPDH (Santa Cruz Biotechnology, Dallas, TX), followed by appropriate HRP-conjugated secondary antibody (Pierce ...
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bioRxiv - Biophysics 2020Quote: ... A mixture of 0.5 µm Alexa Fluor 488 lysine labeled NSARS-CoV-2 and 1:100 Alexa Fluor 594 conjugated G3BP1 antibody (Santa Cruz Biotechnology, sc-365338 AF594) in cell permeabilization buffer was added to the cells and movies were recorded with 512 x 512 pixel resolution at 1000 Hz speed and 1 s per frame for about 2-3 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... was diluted 1:10000 in 5% non-fat dry milk in PBST1X and incubated 1h at RT and subsequently with Goat anti-mouse-HRP (Santa Cruz, Dallas, TX, USA-A1303) diluted 1:1000 in 2% non-fat dry milk in PBST1X 30min RT.
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies were used in the indicated dilutions: anti-α-tubulin (1:1000, B-5-1-2, Santa Cruz, USA, cat no sc-23948), anti-β-actin (1:10000 ...
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bioRxiv - Neuroscience 2020Quote: ... or TBS with 5% bovine serum albumin (BSA) followed by antibody incubation: sEH (1:300, Santa Cruz or 1:1000, Dr. Bruce Hammock’s lab (26)) ...
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bioRxiv - Cell Biology 2020Quote: ... Oil Red O (OrO) working solution was prepared: OrO stock solution (generated by mixing 0.3g Oil Red O powder, Santa Cruz Biotech. CAS# 1320-06-5) was dissolved into 100ml of 100% isopropanol ...
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bioRxiv - Developmental Biology 2020Quote: ... Biotinylated species-specific secondary antibodies were diluted in PBS with 5% GS and applied to tissues for 1 hr at room temperature (Santa Cruz, #sc-2041, 1:250). Staining was detected using the VectaStain ABC Elite Kit (#PK-6100 ...
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bioRxiv - Cancer Biology 2024Quote: ... and blocked in 5% milk in TBS-T before incubation overnight with one of the following primary antibodies: anti-p53 (Santa Cruz Biotechnology, Cat# sc-126), anti-RPL22 (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were blocked with 5% milk/TBST for 30 min and incubated with primary antibodies/TBST against proteins of interest: anti-RBM12 (Santa Cruz Biotechnology, Cat. #sc-514258), anti-PKA alpha polyclonal antibody (Thermo Fisher ...
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bioRxiv - Physiology 2020Quote: ... The membrane was blocked with 5% skim milk (Morinaga, Japan) for 60 min and incubated with the antibody against Epo (Santa Cruz, sc-5290, 1:500-2,000) for 60 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with a rabbit polyclonal anti-VP1 antibody (Ma, et al., 2020) or anti-guanylate binding protein (GBP) 1–5 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing ...
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bioRxiv - Cancer Biology 2023Quote: ... Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2, sc-233X, Santa Cruz; POU2AF1, sc-955X, Santa Cruz; p300, A300-358A, Bethyl). ATAC-Seq was performed as previously described10 with nuclei isolated from 50,000 cells for each sample ...
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bioRxiv - Biochemistry 2023Quote: ... was blocked in 5% milk for one hour at room temperature and incubated with a monoclonal mouse anti-GFP antibody (Santa Cruz Biotechnology sc-9406, 1:2500), a rabbit anti-pRab8A phospho T72 monoclonal antibody (Abcam 230260 ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were stripped by incubation with 2 M NaOH for 5 min and reprobed with anti-β-tubulin (sc-55529 Santa Cruz Biotechnology, 1:1 000) and sheep anti-mouse HRP conjugate antibody (NA 931 Amersham ...
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bioRxiv - Molecular Biology 2020Quote: ... the PVDF membrane was blocked with 5% (w/v) skimmed milk followed by an incubation with a primary antibody synthesized in mouse against GFP (Santa Cruz Biotechnology, CAT. #sc-9996; 1:600) or HA (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% CO2.GIV knock-out (KO) cell lines were generated using Pooled guide RNA plasmids (commercially obtained from Santa Cruz Biotechnology; Cat# sc-402236-KO-2), as described earlier(67) ...
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bioRxiv - Cancer Biology 2023Quote: ... The middle part of the membrane was blocked with 5% bovine serum albumin (BSA) in TBST followed by incubation with mouse monoclonal anti-β-Actin (Santa Cruz Biotechnology, USA; #47778, 1:10,000) primary antibody at RT for 1 h as loading control ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were washed three times for 5 min with PBS and then covered with m-lgG1BP-CFL 594 (Santa Cruz Biotechnology, Inc., TX, USA; 1:300) diluted in 1.5% BSA for 1 h at room temperature in the dark ...