PD 1 Mouse HEK293 His citations for Santa Cruz

Quote: ... Mouse monoclonal integrin β1-activating (TS2 /16) and mouse monoclonal integrin α2 (P4B4) antibodies were purchased from Santa Cruz Biotechnology ...

A poxvirus decapping enzyme localizes to mitochondria to regulate RNA metabolism and translation, and promote viral replication

Shuai Cao, et al., bioRxiv - Microbiology 2021

Quote: ... Mouse α-Tom20 antibody (sc-17764) and mouse α-GAPDH antibody (sc-365062 HRP) were purchased from Santa Cruz Biotechnology ...

Talin mechanosensitivity is modulated by a direct interaction with cyclin-dependent kinase-1

Rosemarie E. Gough, et al., bioRxiv - Cell Biology 2021

Quote: ... or immunoprecipitating mAbs (mouse anti–cyclin B1 clone GNS3 or mouse anti–cyclin A clone E67.1; SC53230; Santa Cruz Biotechnology ...

Synergistic effect of mutations in the Parkinson’s disease-linked endocytic proteins synaptojanin 1 (PARK20) and auxilin (PARK19)

Xin Yi Ng, et al., bioRxiv - Neuroscience 2022

Quote: ... mouse anti-β-actin (sc-47778, RRID: AB_2714189) and mouse anti-Hsc70 (sc-7298, RRID: AB_627761) from Santa Cruz Biotechnology ...

Rab10 regulates the sorting of internalised TrkB for retrograde axonal transport

Oscar M. Lazo, Giampietro Schiavo, bioRxiv - Neuroscience 2022

Quote: ... The levels of immunoprecipitated Rab10 and KIF13B were analysed by western blot using mouse anti-HA (Cancer Research UK, Cat. no. 12CA5, RRID:AB_2920713) and mouse anti-GFP B-2 (Santa Cruz Biotechnologies ...

ZFAND6 is a subunit of a TRAF2-cIAP E3 ubiquitin ligase complex essential for mitophagy

Kashif Shaikh, et al., bioRxiv - Immunology 2024

Quote: ... Antibodies used for confocal microscopy are: Alexa Fluor 647 mouse anti-mouse LAMP1 (Santa Cruz Cat# sc-20011 AF647), dsDNA mouse mAb (Santa Cruz Cat# sc-58749) ...

Targeting NGF but not VEGF or BDNF signaling reduces endometriosis-associated pain in mice

Tiago H. Zaninelli, et al., bioRxiv - Pharmacology and Toxicology 2023

Quote: ... following overnight incubation at 4°C with primary antibodies: mouse anti-mouse phosphorylated-NF-κB (pNF-κB, Santa Cruz Biotechnology ...

Sonogenetic control of mammalian cells using exogenous Transient Receptor Potential A1 channels

Marc Duque, et al., bioRxiv - Neuroscience 2021

Quote: ... cells were incubated overnight at 4°C with different primary antibodies: for HEK cells a mouse monoclonal αTRPA1 antibody (1:1000; Santa Cruz Biotech #376495) was used ...

Telomere elongation in the gut extends zebrafish lifespan

Mounir El Maï, et al., bioRxiv - Cell Biology 2022

Quote: ... The following primary antibodies were used: mouse monoclonal antibody against Proliferation Cell Nuclear Antigen (PCNA, sc56 Santa Cruz, CA, USA, 1:50 dilution) and rabbit polyclonal against myeloperoxidase (MPX ...

High Fat Diet-Induced Obesity Negatively Affects Whole Bone Bending Strength but not Cortical Structure in the Femur

Nicholas J. Hanne, et al., bioRxiv - Bioengineering 2019

Quote: ... Sections were stained overnight at 4°C using unconjugated primary antibodies at 1:100 dilution for endomucin (rat anti-mouse sc-65495, Santa Cruz Biotechnology, Santa Cruz, CA) and at 1:200 dilution for collagen type I (rabbit anti-mouse ...

The neural G protein Gα_o tagged with GFP at an internal loop is functional in C. elegans

Santosh Kumar, Andrew C. Olson, Michael R. Koelle, bioRxiv - Neuroscience 2021

Quote: ... Blots were reprobed for Gβ by stripping and then incubating overnight with 1:200 diluted mouse monoclonal anti-Gβ antibody (Santa Cruz #sc-166123) followed by a secondary incubation with 1:1000 diluted m-IgGk BP-HRP (Santa Cruz #sc-516102) ...

CRISPR/Cas9 technology abolishes the BCR/ABL1 oncogene in chronic myeloid leukemia and restores normal hematopoiesis

Elena Vuelta, et al., bioRxiv - Cancer Biology 2020

Quote: BCR-ABL protein expression was assessed by SDS-PAGE and western blotting using a mouse anti-ABL antibody (1:1,000, Santa Cruz Biotechnology, CA, USA). Horseradish peroxidase-conjugated α-mouse antibody (1:10,000 ...

Elevated soluble Galectin-3 as a marker of chemotherapy efficacy in Breast cancer patients; a prospective study

Arooj Shafiq, et al., bioRxiv - Cancer Biology 2020

Quote: ... Gal-3 and Ki67 expression was detected by IHC using mouse polyclonal anti-Gal-3 (Santa Cruz, CA, USA, SC-14364, 1:500) and rabbit polyclonal anti-Ki67 (abcam ...

The spike protein of SARS-CoV-2 induces endothelial inflammation through integrin α5β1 and NF-κB

Juan Pablo Robles, et al., bioRxiv - Cell Biology 2021

Quote: ... and incubated overnight at 4°C in a humid chamber with 1:200 mouse monoclonal anti-NF-κB p65 antibody (Santa Cruz, CA, USA) in 0.1% Tx-100 ...

Novel fragile X syndrome 2D and 3D brain models based on human isogenic FMRP-KO iPSCs

Carlo Brighi, et al., bioRxiv - Neuroscience 2020

Quote: ... Tissues were then incubated overnight with primary antibodies in 0.3% Triton-X and 5% goat serum in PBS at the following dilutions: mouse anti-PAX6 (sc81649 Santa Cruz Biotechnology, 1:50), rabbit anti-FOXG1 (ab18259 Abcam ...

Elevated dietary ω-6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditions

Jacob T. Boyd, et al., bioRxiv - Neuroscience 2020

Quote: ... Actin was measured as a loading control with a monoclonal mouse anti-actin antibody (1:1000, sc-47778, Santa Cruz; Santa Cruz, CA). Donkey anti-rabbit IR800 and donkey anti-mouse IR680 secondary antibodies (1:10,000 ...

Elevated dietary ω-6 polyunsaturated fatty acids induce reversible peripheral nerve dysfunction that exacerbates comorbid pain conditions

Jacob T. Boyd, et al., bioRxiv - Neuroscience 2020

Quote: ... Actin was measured as a loading control with a monoclonal mouse anti-actin antibody (1:1000, sc-47778, Santa Cruz; Santa Cruz, CA). Donkey anti-rabbit IR800 and donkey anti-mouse IR680 secondary antibodies (1:10,000 ...

Human immunodeficiency virus type 2 capsid protein mutagenesis reveals amino acid residues important for virus particle assembly

Huixin Yang, et al., bioRxiv - Microbiology 2022

Quote: The efficiency of immature particle production was analyzed by quantifying Gag proteins in released particles by harvesting cell culture supernatants by using immunoblot analysis with a mouse monoclonal anti-HIV-1 p24 antibody (Catalogue #: sc-69728; Santa Cruz Biotechnology, TX). Briefly ...

Imbalance of Neuregulin1-ErbB2/3 signaling underlies altered myelin homeostasis in models of Charcot-Marie-Tooth disease type 4H

Lara El-Bazzal, et al., bioRxiv - Neuroscience 2022

Quote: ... The membrane was then incubated overnight with the following primary antibodies: mouse anti-Neuregulin1α/β1/2 (D10) (1:500) (#sc-393009; Santa Cruz Biotechnology, USA), rabbit anti-phospho-Akt (Ser473 ...

Cytosolic sequestration of spatacsin by Protein Kinase A and 14-3-3 proteins

Susanna Cogo, et al., bioRxiv - Cell Biology 2022

Quote: ... The antibodies and dyes used for ICC are as follows: mouse α-pan 14-3-3 (sc-1657, Santa Cruz Biotechnology, 1:100), mouse α-Flag® M2 (F1804 ...

Hrq1/RECQL4 regulation is critical for preventing aberrant recombination during DNA intrastrand crosslink repair and is upregulated in breast cancer

Thong T. Luong, et al., bioRxiv - Genetics 2022

Quote: The following primary and secondary antibodies were used at the indicated dilutions: Myc (mouse monoclonal, Santa Cruz Biotechnology #sc-40, 1:500, WB); Clb2 (rabbit polyclonal ...

Neuroepithelial progenitors generate and propagate non-neuronal action potentials across the spinal cord

Kalaimakan Hervé Arulkandarajah, et al., bioRxiv - Neuroscience 2020

Quote: ... The following primary antibodies were used: a mouse monoclonal anti-HNF-3β/FoxA2 (H-4) antibody sc-374376 (1:300, Santa Cruz Biotechnology, USA), a mouse monoclonal anti-Nkx2.2 antibody 74.5.A5 (1:600 ...

Tolerogenic Nanoparticles Impacting B and T Lymphocyte Responses Delay Autoimmune Arthritis in K/BxN Mice

Amrita Srivastava, et al., bioRxiv - Immunology 2020

Quote: ... Plates were then washed 4 times and incubated with anti-IgG1 mouse-horseradish peroxidase (HRP) conjugate (1:2000 dilution; Santa Cruz Biotechnology Inc.) at RT for 1 h ...

Novel newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye

Abijeet Singh Mehta, et al., bioRxiv - Developmental Biology 2021

Quote: ... Followed by 1h incubation with secondary antibody 1:5000 horseradish peroxidase conjugated goat anti-mouse IgG-HRP (Santa Cruz Biotechnology, Cat. Sc-2005). The signal in both the blots were detected using Super Signal West Dura Extended Duration Substrate (ThermoFisher Scientific ...

Structural basis for human T-cell leukemia virus type 1 Gag targeting to the plasma membrane for assembly

Dominik Herrmann, et al., bioRxiv - Microbiology 2021

Quote: ... HTLV-1 Gag expression in cells and VLPs in the cell culture supernatant was detected using a mouse monoclonal antibody HTLV-1 p24 (6G9) (Santa Cruz Biotechnology, TX) 1:3,000 and secondary antibody goat anti-mouse IgG StarBright Blue 700 (Bio-Rad ...

How scars shape the neural landscape: key molecular mediators of TGF-β1’s anti-neuritogenic effects

Kye-Im Jeon, Krystel R. Huxlin, bioRxiv - Neuroscience 2020

Quote: ... to detect and quantify the expression of p-GSK-3β relative to that of mouse monoclonal (total) t-GSK-3α/β (1:2000; SC-7291 Santa Cruz Biotechnology).

How scars shape the neural landscape: key molecular mediators of TGF-β1’s anti-neuritogenic effects

Kye-Im Jeon, Krystel R. Huxlin, bioRxiv - Neuroscience 2020

Quote: ... we probed for changes in the expression of signaling molecules that could mediate the anti-neuritogenic effects of TGF-β1 on ND7/23 cells: total (t)-GSK-3α/β (mouse monoclonal antibody, 1:2000; sc-7291, Santa Cruz Biotechnology), and t-CRMP2 (mouse monoclonal antibody ...

Synaptotagmins Maintain Diacylglycerol Homeostasis at Endoplasmic Reticulum-Plasma Membrane Contact Sites during Abiotic Stress

Noemi Ruiz-Lopez, et al., bioRxiv - Molecular Biology 2020

Quote: ... The following primary antibodies were used for detection of epitope-tagged proteins: mouse monoclonal anti-GFP clone B-2 (1:1000, catalog no. sc-9996, Santa Cruz Bio-technology) and rabbit polyclonal anti-SYT1 antibody (1:5000) ...

A Systems Approach Identifies Enhancer of Zeste Homolog 2 (EZH2) as a Protective Factor in Epilepsy

Nadia Khan, et al., bioRxiv - Neuroscience 2019

Quote: ... membranes were washed three times with 1X TBST and incubated with horseradish peroxidase-conjugated goat anti-rabbit or goat anti-mouse IgG secondary antibodies (1:10,000, Santa Cruz Biotech, Dallas, TX) for 1 hour at room temperature ...

TET2-mediated epigenetic reprogramming of breast cancer cells impairs lysosome biogenesis

Audrey Laurent, et al., bioRxiv - Cancer Biology 2021

Quote: ... Membranes were incubated overnight with an anti dsRNA antibody (dsRNA mAb J2, Scicons) diluted 1/500 followed by an anti-mouse antibody coupled to horseradish peroxidase (Santa Cruz, sc-2005) diluted 1/10000 ...

Redistribution of the novel C. difficile spore-adherence receptor, E-cadherin, by TcdA and TcdB increases spore-binding to adherens junctions

Pablo Castro-Córdova, et al., bioRxiv - Microbiology 2022

Quote: ... Unbonded E-cadherin was washed 5 times with 150 µL PBS–Tween and was incubated with 50 µL of 1:50 IgG of mouse anti–E-cadherin (SC8426-Santa Cruz Biotechnology, USA) in PBS–Tween–1% BSA for 1 h at 37 °C ...

The genome-wide mutational consequences of DNA hypomethylation

Nicolle Besselink, et al., bioRxiv - Genomics 2022

Quote: ... Western Blot was blocked using 5% ELK and incubated overnight with primary antibody mouse monoclonal anti-UHRF1 (Santa Cruz, SC-373750, 1:250) or rabbit polyclonal anti-DNMT1 (Invitrogen ...

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