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Citations for Santa Cruz :
1801 - 1850 of 1892 citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ATDC5 and SaOS-2 cells were transfected with the mouse or human SHIP2 double nickase plasmid (Santa Cruz, sc-421138-NIC (mouse), sc-401622-NIC (human) ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was harvested at 48 and 72 hours after transfection in RPMI media supplemented with 10% FBS and filtered with 45 μm filters prior to transduction of cancer cell lines using centrifugation at 1000 g for 2 hours in the presence of 8 μg/mL of polybrene (Santa Cruz Biotechnology). Transduced cell lines were selected in puromycin and/or blasticidin (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining cell suspension was then incubated in a positive-selection plate coated with anti-PDGFRα antibodies (catalog no. sc-338; Santa Cruz Biotechnology). The attached cells were collected using trypsin and cultured on poly(L-lysine)-coated coverslips in proliferation medium containing Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000, 71G4B7, Cell Signaling; beta actin 1:5000 sc-4778, Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies for assay of infected cells were chicken polyclonal anti-UL34 (1:250) (71) and mouse monoclonal anti-LMNA/C (Santa Cruz Biotechnology), both diluted 1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentivirus-containing media was centrifuged and added to MDA-MB-468 cells for shRNA transduction with Polybrene (8 μg/mL, # sc-134220A, Santa cruz biotechnology). Then ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting cell pellets were then incubated with room temperature PBS containing 2 mM DSG (disuccinimidyl glutarate, sc-285455, Santa Cruz Biotechnology) for 25 min with gentle agitation ...
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bioRxiv - Microbiology 2024Quote: ... Villin was used as cell loading control and detected using 1:1000 dilution of mouse anti-villin (#sc-373997, Santa Cruz Biotechnology) antibody.
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bioRxiv - Cancer Biology 2024Quote: ... BUB3 and TTK genes in PANC-1 was achieved by transfecting cells with short hairpin RNA (shRNA)-packed lentiviral particles (Santa Cruz Biotechnology). In a 6-well plate ...
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bioRxiv - Cancer Biology 2024Quote: Rab27B was knocked out in the human colon cancer cell line HCT116 using Rab27B CRISPR/Cas9 KO plasmid (sc-403498; Santa Cruz Biotechnology), followed by insertion of a homology-directed repair (HDR ...
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bioRxiv - Genomics 2024Quote: ... lentiviral transduction was performed by mixing cells in media supplemented with 8 µg/mL of polybrene (Santa Cruz Biotechnology 28728-55-4) with lentivirus and centrifuging in cell culture plates at 1000 xg and 33 °C for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... HCT116 Wild type (WT) and ΔADH5 cells were plated in P60 dishes allowed to adhere and then treated with mitomycin C (MMC, Santa Cruz, #sc-3514) 0.5 μg/ml during 24 h or with FA 150 μM during 48 h ...
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bioRxiv - Immunology 2021Quote: ... The cells were resuspended in the lentivirus-containing medium with addition of 8 μg/ml polybrene (Santa Cruz Biotechnology, Cat. no: sc-134220) and centrifuged for 90 min at 2,500 g at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: A previously published immunofluorescence-fluorescence in situ hybridization protocol was carried out on cells seeded on sterile glass coverslips and tumor sections.43 Coverslips were incubated with PML antibody (Santa Cruz, sc-5621) diluted 1:200 for one hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C with different primary antibodies: for HEK cells a mouse monoclonal αTRPA1 antibody (1:1000; Santa Cruz Biotech #376495) was used ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: mouse monoclonal antibody against Proliferation Cell Nuclear Antigen (PCNA, sc56 Santa Cruz, CA, USA, 1:50 dilution) and rabbit polyclonal against myeloperoxidase (MPX ...
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bioRxiv - Molecular Biology 2021Quote: ... The culture media was filtered through a 0.45-μm filter and added to A549 cells along with Polybrene (4 μg/ml; Santa Cruz Biotechnology, Dallas, TX). At 72 h after transduction ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×105 cells were infected in the presence of 1.5 ml of viral supernatant and 4 μg/ml polybrene (Santa Cruz Biotechnology, SC-134220).
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bioRxiv - Cancer Biology 2020Quote: ... Cas9-expressing cell lines were infected at a density of 2×105 cells in 1.5 mL media in the presence of 4 μg/mL polybrene (Santa Cruz Biotechnology, SC-134220). sgRNA resistant human ZRSR2 DNA fragment was synthesized by Twist Bioscience and cloned into pRRL.idTomato.
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bioRxiv - Microbiology 2020Quote: ... 1 ml of the bacterial suspensions (108 cells mL−1 was added under constant agitation, thus obtaining the mixed inoculants. Tomato (Santa Cruz, giant Kada) and papaya (Caliman-01 - UENF ...
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bioRxiv - Microbiology 2022Quote: The efficiency of immature particle production was analyzed by quantifying Gag proteins in released particles by harvesting cell culture supernatants by using immunoblot analysis with a mouse monoclonal anti-HIV-1 p24 antibody (Catalogue #: sc-69728; Santa Cruz Biotechnology, TX). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by an overnight incubation at 4°C with Rabbit-anti-NANOG (1/800, Cell Signalling, #3580S) and Mouse-anti-OCT3/4 (1/250, Santa Cruz, sc-5279) antibodies diluted in BB supplemented with 0.1% Triton-X ...
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bioRxiv - Neuroscience 2020Quote: ... we probed for changes in the expression of signaling molecules that could mediate the anti-neuritogenic effects of TGF-β1 on ND7/23 cells: total (t)-GSK-3α/β (mouse monoclonal antibody, 1:2000; sc-7291, Santa Cruz Biotechnology), and t-CRMP2 (mouse monoclonal antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used: goat anti-LCN2 (R&D AF1747), E-cadherin (Cell Signal, 3195T) IL-6 (Santa Cruz Biotechnology, sc-1265).
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bioRxiv - Microbiology 2019Quote: ... The membranes were then incubated with primary antibody (anti-pSTAT1 Y701 [clone D4A7; Cell Signaling Technologies; 1:100 dilution], anti-STAT1 p84/p91 [clone E23; Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... fixed cells were double immunolabeled with rabbit polyclonal anti-K17 (McGowan et al 1998) and mouse anti-K17 (Santa Cruz REF# sc-393002) prior to incubation with PLA probes (Sigma-Aldrich REF# DUO92002 and DUO92004 ...
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bioRxiv - Microbiology 2020Quote: ... Slides were blocked in normal serum and incubated in primary antibody (USP13 (D4P3M; 12577, Cell signalling Technologies (CST)) or Mcl-1 (S-19; sc-819, Santa Cruz Biotechnology (SCBT)) overnight at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were incubated with goat anti-rabbit IgG phycoerythrin-R (PE) conjugated antibody (1:200, Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were incubated with goat anti-rabbit IgG phycoerythrin-R (PE) conjugated antibody (1:200, Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Luc-KPV+/+ cells were transfected with a commercially available CRISPR/Cas9 vimentin knockout plasmid according to manufacturer’s directions (Santa Cruz Biotechnology sc-423676).
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bioRxiv - Cancer Biology 2022Quote: Western blotting of nuclear extracts or whole cell extracts was performed by standard methods using antibodies specific for the N-terminal portion of ETV6 (Sigma, # HPA000264; Santa Cruz, #sc-166835x), CTCF (Cell Signaling ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... solvent control and drug treatment groups with Trypsin-EDTA (2X) and single cell suspensions were stained using an antibody against TMPRSS2 (Santa Cruz, sc-515727). Samples were fixed with 4 % paraformaldehyde which was followed by permeabilization step or 15 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Preclearing and washing cell lysate were performed using 20ul A/G PLUS-Agarose beads per 200ul sample (Cat#SC2003; Santa Cruz Biotechnology, USA) for 4 hrs followed by centrifugation at 15,000g for 10 minutes at 4°c ...
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bioRxiv - Immunology 2022Quote: ... the blotted membranes were then incubated overnight with primary antibodies (anti-Blimp1 (C14A4, 1:1,000, Cell Signaling Technologies or C-7, 1:200, Santa Cruz Biotechnology), anti-β-actin (A5441 ...
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: ... which were diluted to 1:100 in 5% BSA and 0.5% Triton X-100 in PBS: anti-BECN1 (#3738; Cell Signaling Technology and sc-48341; Santa Cruz Biotechnology), anti-PLIN1 (#9349 ...
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bioRxiv - Cancer Biology 2023Quote: ... cell lysates were first incubated with anti-TNFAIP2/ITGB4/IQGAP1 antibodies or mouse IgG/rabbit IgG (sc-2028;Santa Cruz Biotechnology, CA, USA) and then incubated with Protein A/G plusagarose beads (sc-2003 ...
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bioRxiv - Immunology 2023Quote: ... After an overnight cells were centrifuged in 400g and re-suspended in OKT3-retroviral in the presence of 8μg/ml Polybrene (Santa Cruz Biotechnology, sc-134220). After 12 hours ...
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bioRxiv - Immunology 2023Quote: Neuro-2a cells were infected with a lentivirus vector encoding 19-25nt (plus hairpin) IL-23R shRNA (Santa Cruz Biotechnology; sc-60835-SH) with 8 μg/ml polybrene ...
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bioRxiv - Developmental Biology 2023Quote: ... with a cocktail of protease inhibitors and the soluble whole cell lysates were immunoprecipitated with an anti-Myc antibody (Santa Cruz, sc-40). The immunoprecipitated proteins were then analysed by Western blot.
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bioRxiv - Genomics 2020Quote: ... the media was replaced with fresh culture media and the cells were allowed to recover for 48 hours before beginning puromycin selection (Santa Cruz Biotechnology #SC-108071). Cells were treated with 800ng/ml puromycin for a total of 72 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... The filtered viral supernatant was used immediately to transduce target cells (PDACc/PANC-1 or fibroblasts) with 10 µg/mL polybrene (Santa Cruz Biotechnology, Dallas, Texas) or stored at −80°C for use in the future ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C with primary antibodies at the following dilutions: mouse anti-PAX6 (sc81649 Santa Cruz Biotechnology, 1:50), mouse anti-GFAP (MAB360 Merck Millipore ...
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bioRxiv - Systems Biology 2021Quote: ... lentivirus was recovered from supernatant using an Amicon Ultra Centrifugal 50kDa filter (MilliporeSigma) and transduced into HeLa cells along with 10 μg/ml polybrene (Santa Cruz Biotechnology, Dallas, TX). Cells were transduced sequentially with PKAR-FKBP-FP followed by Lyn-FRB ...
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bioRxiv - Cell Biology 2020Quote: ... and β5-integrin cells were incubated for 20 min before chase and during pulse with 10 μM gefitinib (Santa Cruz Biotechnology, 184475-35-2), 10 μM PP2 (Thermo-Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... was used for generation of the A375-IVLuc cell line by antibiotic selection (Hygromycin b, 200 μg/ml, Santa Cruz Biotechnology, 31282-04-9) and the limiting dilution method for clonal lines generation ...
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bioRxiv - Cell Biology 2022Quote: ... ChIP for human endogenous GATA4 in SH-SY5Y cells was carried out using the GATA-4 (G-4) (Cat. sc-25310, Santa Cruz, 1:50, RRID:AB_627667). All primers for qRT-PCR or ChIP-qPCR are as indicated in Table S3.
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bioRxiv - Molecular Biology 2019Quote: ... 5 μg of antibody (anti-PUM1, Santa Cruz sc-135049; anti-PUM2, Santa Cruz sc-31535; anti-RBMX Cell Signaling #14794; Goat IgG control, Santa Cruz sc-2028 ...
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bioRxiv - Cancer Biology 2019Quote: ... αKO KPC cells lacking CD80 (referred to as αKO/CD80KO) were generated by transfecting αKO cells with B7-1 CRISPR/Cas9 KO plasmid (Santa Cruz Biotechnology, sc-419570). 48 hours after transfection ...
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bioRxiv - Pathology 2021Quote: ... the membranes were incubated in a blocking buffer containing the appropriate diluent of primary antibodies against apoA5 (#3335, Cell Signaling Technology, USA; sc-373950, Santa Cruz, USA), tubulin (66031-1-lg ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies (anti-human IL-17RA antibody, Cell Signaling Technology, Danvers, MA, USA; anti-human IL-1Rrp2 antibody, Santa Cruz Biotechnologies, Santa Cruz, USA) were used diluted 1:1000 and applied for 18 hours followed by the appropriate horseradish peroxidase-coupled secondary antibody (GE Bioscience).