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Citations for Santa Cruz :
1601 - 1650 of 1669 citations for 6H Thieno 2 3 b pyrrole 5 carboxylicacid ethyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was harvested at 48 and 72 hours after transfection in RPMI media supplemented with 10% FBS and filtered with 45 μm filters prior to transduction of cancer cell lines using centrifugation at 1000 g for 2 hours in the presence of 8 μg/mL of polybrene (Santa Cruz Biotechnology). Transduced cell lines were selected in puromycin and/or blasticidin (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... The reaction was incubated on ice for 10 min and super-shift assay was performed by adding 2 µL of anti-HuR antibody (3A2, mouse monoclonal IgG antibody, Santa Cruz Biotechnology). After antibody addition ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was centrifuged to remove debris and the supernatant was rocked at 4°C for 2 h with an anti-Flag antibody (anti-DDDDK tag, MBL Life Science) or mouse anti-IgG antibody (Santa Cruz Biotechnology) that was conjugated with ‘Dynabeads Protein A’ (Veritas ...
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bioRxiv - Molecular Biology 2024Quote: ... the cell pellet (corresponding to 2 × 107 cells) was lysed in 500 µL of RIPA Lysis buffer (Santa Cruz Biotechnology, sc-24948) and sonicated to reduce sample viscosity from lysed DNA ...
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bioRxiv - Genomics 2019Quote: ... or 7.5 ug (Oct4) antibody overnight at 4’C (antibodies: H3K9me3: abcam AB8898; H3K27Ac: Active Motif 39133; H3K4me3: Active Motif 39159; Oct4: Santa Cruz sc-8629). Antibody-bound chromatin was incubated with Protein G Dynabeads (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were diluted 1:5 in RIPA buffer and incubated overnight at 4°C with 1 µg/ml myogenin antibody (Santa Cruz Biotechnology, #sc-12732 X) or IgG1 isotype control (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... was diluted 1:10000 in 5% non-fat dry milk in PBST1X and incubated 1h at RT and subsequently with Goat anti-mouse-HRP (Santa Cruz, Dallas, TX, USA-A1303) diluted 1:1000 in 2% non-fat dry milk in PBST1X 30min RT.
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bioRxiv - Biochemistry 2022Quote: ... The membrane is washed three times with PBST for 5 min and then incubated for 1 h at 25 C with a primary anti-dynein antibody (sc-13524) (Santa Cruz Biotechnology, Dallas, TX, USA) diluted 1:200 (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... or TBS with 5% bovine serum albumin (BSA) followed by antibody incubation: sEH (1:300, Santa Cruz or 1:1000, Dr. Bruce Hammock’s lab (26)) ...
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bioRxiv - Zoology 2019Quote: Protein was extracted from 5 live larvae using the methanol/chloroform protocol [33] and solubilized in electrophoresis sample buffer (Santa Cruz Biotechnology, Dallas, TX, USA) for SDS-polyacrylamide gel electrophoresis and Western Blot analysis ...
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bioRxiv - Neuroscience 2021Quote: ... The membrane was blocked for 1 h in PBS-T supplemented with 5% skim milk and afterward incubated with 1:600 primary c-Myc-tag Polyclonal antibody (sc-789, Santa Cruz Biotechnology, Dallas, TX, USA) in PBS-T containing 1% skim milk over night at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Oil Red O (OrO) working solution was prepared: OrO stock solution (generated by mixing 0.3g Oil Red O powder, Santa Cruz Biotech. CAS# 1320-06-5) was dissolved into 100ml of 100% isopropanol ...
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bioRxiv - Developmental Biology 2020Quote: ... Biotinylated species-specific secondary antibodies were diluted in PBS with 5% GS and applied to tissues for 1 hr at room temperature (Santa Cruz, #sc-2041, 1:250). Staining was detected using the VectaStain ABC Elite Kit (#PK-6100 ...
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bioRxiv - Cancer Biology 2024Quote: ... and blocked in 5% milk in TBS-T before incubation overnight with one of the following primary antibodies: anti-p53 (Santa Cruz Biotechnology, Cat# sc-126), anti-RPL22 (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 0.5-1x105 cells were seeded on coated cover glasses for 30 minutes and then fixed with chilled 4% paraformaldehyde (Santa Cruz Biotechnology, Dallas, TX, USA) for 10 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were blocked with 5% milk/TBST for 30 min and incubated with primary antibodies/TBST against proteins of interest: anti-RBM12 (Santa Cruz Biotechnology, Cat. #sc-514258), anti-PKA alpha polyclonal antibody (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein extracts were incubated with each antibody with rotation for 2 hr and then added to protein A/G agarose beads (Santa Cruz, sc-2003) for an additional 1 hr ...
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bioRxiv - Immunology 2021Quote: ... JUND (D-9, sc-271938), SMAD3 (38-Q, sc-101154), MAX (H-2, sc-8011), and MYC (9E10, sc-40) (all, Santa Cruz Biotechnology, USA) followed by peroxidase-conjugated anti-mouse antibodies (NA931 ...
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bioRxiv - Molecular Biology 2020Quote: Mouse monoclonal primary antibodies included: anti-CD63 (clone MX-49.129.5, 2 μg/mL, cat. no. sc-5275, Santa Cruz Biotechnology, Dallas, TX, USA), anti-CD81 (clone 1.3.3.22 ...
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bioRxiv - Molecular Biology 2020Quote: ... were washed with 200 μL HBS (150 mM NaCl, 10 mM HEPES, pH7.5 by KOH) and incubated with 2 μg anti-HA (Santa Cruz, Cat.# sc-7392) or 2 μg rabbit IgG isotype (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... all media was removed from the plate and replaced with 8 ml of 2 µg/ml puromycin (Santa Cruz Biotech cat # sc-108071) L-15 selection media ...
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bioRxiv - Developmental Biology 2020Quote: ... at 1:200, anti-monomeric Kusabira-Orange 2 (rabbit, MBL amalgam PM051M) at 1:250 and anti-p63 (mouse, Santa Cruz Biotechnology 4A4) at 1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cas9-expressing cell lines were infected at a density of 2×105 cells in 1.5 mL media in the presence of 4 μg/mL polybrene (Santa Cruz Biotechnology, SC-134220). sgRNA resistant human ZRSR2 DNA fragment was synthesized by Twist Bioscience and cloned into pRRL.idTomato.
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bioRxiv - Neuroscience 2022Quote: ... The membrane was then incubated overnight with the following primary antibodies: mouse anti-Neuregulin1α/β1/2 (D10) (1:500) (#sc-393009; Santa Cruz Biotechnology, USA), rabbit anti-phospho-Akt (Ser473 ...
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bioRxiv - Physiology 2021Quote: ... Kidney cortex cryosections were labeled with a polyclonal goat anti-aquaporin-2 (Aqp2) antibody (0.2 μg/ml, sc-9882; Santa Cruz Biotechnology, Santa Cruz CA, USA), and detected using a donkey anti-goat immunoglobulin G conjugated to Cyanine Cy3 (0.5 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 5% skimmed milk powder for 2 hours at room temperature.Incubate primary antibody overnight at 4°C.The membrane was washed three times with TBST and then incubated at room temperature with conjugated HRP secondary antibodies for 2 hours.ECL (enhanced chemiluminescence kit, Santa Cruz Biotechnology, Dallas, TX) detection system (Thermo Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were then stained overnight at 4°C in 2% BSA in DPBS with primary antibodies: anti-MUC2 (H-9, sc-515106, Santa Cruz, 1:100), anti-E-Cadherin (HECD-1 ...
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bioRxiv - Genomics 2019Quote: ... RNA Pol II and NF-YA levels were measured using an anti-Rpb3 antibody60 and anti-NF-YA antibody (G-2; Santa Cruz, sc-17753X) respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... overnight rotating at 4 °C together with either α-KAP1 or α-RIF1 antibodies (see Supplementary Table 2) or IgG only control (#sc-2026, Santa Cruz), 10% of chromatin was isolated as input control ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2-S1 protein produced in HEK cells (Creative Diagnostics, DAGC091) was covalently labeled using CruzFluor647 (Santa Cruz Biotechnology, sc-362620) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were passaged as needed in the presence of 2 μM Rho-associated kinase (ROCK) inhibitor (RI) thiazovivin (Santa Cruz Biotechnology, #SC-361380). Prior to transfection ...
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bioRxiv - Cell Biology 2022Quote: ... plates were flooded with detection solution containing 0.1 mg mL−1 2-naphthyl sulfate (potassium salt; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) as a chromogenic substrate coupled with 1 mg mL−1 tetrazotized-o-dianisidine chloride (Fast Blue B salt ...
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bioRxiv - Cell Biology 2024Quote: ... 0.2% Triton X-100 in 1XTBS) for 1 h at RT followed by incubation with primary antibodies against ERG (Santa Cruz, 1:100) for 72 h and EC marker ...
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bioRxiv - Cell Biology 2023Quote: ... Volumes of lysate containing equal amounts of total protein were used to immunoprecipitate PARP14 by incubating with 2 µg of a mouse monoclonal antibody against PARP14 (Santa Cruz, sc-377150), and protein G agarose beads overnight at 4°C with gentle mixing on a nutator at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The sections were blocked and incubated for 2 days at 4°C with the following primary antibodies: mouse anti-MBP (sc-66064, Santa Cruz Biotechnology Inc.), goat anti-OLIG2 (Bio-Techne ...
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bioRxiv - Physiology 2020Quote: ... The membrane was blocked with 5% skim milk (Morinaga, Japan) for 60 min and incubated with the antibody against Epo (Santa Cruz, sc-5290, 1:500-2,000) for 60 min at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... dried and blotted overnight in 5% BSA TBS-T at 4 °C using primary antibodies (Abcam anti-SMARCD3: ab171075; Santa Cruz anti-β Actin (C-4): sc-47778 ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with a rabbit polyclonal anti-VP1 antibody (Ma, et al., 2020) or anti-guanylate binding protein (GBP) 1–5 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing ...
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bioRxiv - Biochemistry 2023Quote: ... was blocked in 5% milk for one hour at room temperature and incubated with a monoclonal mouse anti-GFP antibody (Santa Cruz Biotechnology sc-9406, 1:2500), a rabbit anti-pRab8A phospho T72 monoclonal antibody (Abcam 230260 ...
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bioRxiv - Molecular Biology 2021Quote: 300 million TetR-CBX7 reporter mESCs were infected with a 1:10 dilution of the harvested virus-containing supernatant PlatinumE cell medium for 24 hrs in the presence of 2 μg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220). The 300 million cells were divided into 3x 100 million sets (10x 15 cm plates of 10 million cells each ...
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bioRxiv - Biophysics 2020Quote: ... using EZ-Retriever V.2 (BioGenex, San Ramon, CA, USA) and immunostained with anti-α-SMA monoclonal antibody (Cat No.sc-32251, Santa Cruz Biotechnology, CA, USA) overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and β5-integrin cells were incubated for 20 min before chase and during pulse with 10 μM gefitinib (Santa Cruz Biotechnology, 184475-35-2), 10 μM PP2 (Thermo-Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed and incubated at room temperature for 2 hours with the appropriate HRP-conjugated secondary antibodies as follows: mouse anti-rabbit (1/1000 dilution; Santa Cruz; cat #sc-2357) and mouse IgGκ BP (1/1000 dilution ...
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bioRxiv - Pathology 2019Quote: ... Cell lysates of equal protein amounts were incubated overnight at 4°C (constant rotation) with 2 μg of an anti-HA antibody or immunoglobulin G (IgG) control (sc2027; Santa Cruz Biotechnology, Dallas, TX). Co-immunoprecipitation of STAT3 with associated proteins was then performed by using Protein A/G Agarose beads included in the Pierce Classic IP Kit (26146 ...
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bioRxiv - Immunology 2019Quote: ... the membrane was re-blocked with 5% BSA in 1x TBST for 2 hour at room temperature before probing with a 1:200 dilution anti-vinculin 7F9 (Santa Cruz Biotechnology; sc-73614) in 5% BSA/1x TBST for 1 hour at room temperature followed by anti-mouse IgG HRP secondary ...
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bioRxiv - Genetics 2021Quote: ... 300 million #H5 clone mESCs were infected with a 1:10 dilution of virus-containing supernatant for 24 h in the presence of 2 μg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220) (Before infection ...
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bioRxiv - Developmental Biology 2024Quote: ... Antibodies used for immunofluorescence were Rat Anti-C-peptide (Developmental Studies Hybridoma Bank, ID-4) and Goat Anti-GLP-2 (Santa Cruz Antibodies, C-20).
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bioRxiv - Immunology 2023Quote: ... Lungs were dissected at the stated time post tumor initiation and fixed for 24 hours in 2% paraformaldehyde (Santa Cruz Biotechnology Cat# sc-253236) at 4°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were removed from dishes and assessed by flow cytometry for construct expression and selected in puromycin 2 μg/mL (Santa Cruz Biotech, sc-108071B). Phoenix-Eco or Phoenix-Eco alpha-V beta-3 were transfected with PB-vector constructs as above ...
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bioRxiv - Cell Biology 2024Quote: Cells in growth medium without penicillin and streptomycin were transfected with the hTRPV4 shRNA plasmid (1 and 2 µg; Santa Cruz sc-61726-SH) and Lipofectamine3000 in opti-MEM ...