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Citations for Santa Cruz :
1601 - 1642 of 1642 citations for 6 Chloro N ethyl 2 methylthio 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... Kidney cortex cryosections were labeled with a polyclonal goat anti-aquaporin-2 (Aqp2) antibody (0.2 μg/ml, sc-9882; Santa Cruz Biotechnology, Santa Cruz CA, USA), and detected using a donkey anti-goat immunoglobulin G conjugated to Cyanine Cy3 (0.5 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 5% skimmed milk powder for 2 hours at room temperature.Incubate primary antibody overnight at 4°C.The membrane was washed three times with TBST and then incubated at room temperature with conjugated HRP secondary antibodies for 2 hours.ECL (enhanced chemiluminescence kit, Santa Cruz Biotechnology, Dallas, TX) detection system (Thermo Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The following primary antibodies were used for detection of epitope-tagged proteins: mouse monoclonal anti-GFP clone B-2 (1:1000, catalog no. sc-9996, Santa Cruz Bio-technology) and rabbit polyclonal anti-SYT1 antibody (1:5000) ...
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bioRxiv - Genomics 2019Quote: ... RNA Pol II and NF-YA levels were measured using an anti-Rpb3 antibody60 and anti-NF-YA antibody (G-2; Santa Cruz, sc-17753X) respectively ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2-S1 protein produced in HEK cells (Creative Diagnostics, DAGC091) was covalently labeled using CruzFluor647 (Santa Cruz Biotechnology, sc-362620) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were passaged as needed in the presence of 2 μM Rho-associated kinase (ROCK) inhibitor (RI) thiazovivin (Santa Cruz Biotechnology, #SC-361380). Prior to transfection ...
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bioRxiv - Cell Biology 2022Quote: ... plates were flooded with detection solution containing 0.1 mg mL−1 2-naphthyl sulfate (potassium salt; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) as a chromogenic substrate coupled with 1 mg mL−1 tetrazotized-o-dianisidine chloride (Fast Blue B salt ...
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bioRxiv - Genetics 2022Quote: ... Once the cells were ∼50% confluent (after 2-3 days) the media was changed to StemFlex with 1ug/uL polybrene (Santa Cruz SC-134220), and 10uL each of Ngn2 and rtTA virus was added to each well ...
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bioRxiv - Molecular Biology 2023Quote: ... catalog number sc7392), α-GFP (clone B-2, catalog number sc9996), and α-ubiquitin (clone P4D1, catalog number sc8017) antibodies were purchased from Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2023Quote: ... Volumes of lysate containing equal amounts of total protein were used to immunoprecipitate PARP14 by incubating with 2 µg of a mouse monoclonal antibody against PARP14 (Santa Cruz, sc-377150), and protein G agarose beads overnight at 4°C with gentle mixing on a nutator at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by fluorescent staining with Pan-Cytokeratin (PanCK, clone AE-1/AE-3, AF488, Santa Cruz NBP2-33200AF488, [2 µg/mL], CA, USA) to identify trophoblasts ...
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bioRxiv - Cell Biology 2024Quote: ... 0.2% Triton X-100 in 1XTBS) for 1 h at RT followed by incubation with primary antibodies against ERG (Santa Cruz, 1:100) for 72 h and EC marker ...
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bioRxiv - Molecular Biology 2021Quote: 300 million TetR-CBX7 reporter mESCs were infected with a 1:10 dilution of the harvested virus-containing supernatant PlatinumE cell medium for 24 hrs in the presence of 2 μg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220). The 300 million cells were divided into 3x 100 million sets (10x 15 cm plates of 10 million cells each ...
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bioRxiv - Plant Biology 2021Quote: ... Membranes were blocked and probed in TBST-5% non-fat milk using anti-GFP (HRP-conjugated B-2, sc-9996 HRP, Santa Cruz, 1:5000 dilution) or anti-HA (HRP-conjugated ...
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bioRxiv - Biophysics 2020Quote: ... using EZ-Retriever V.2 (BioGenex, San Ramon, CA, USA) and immunostained with anti-α-SMA monoclonal antibody (Cat No.sc-32251, Santa Cruz Biotechnology, CA, USA) overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and β5-integrin cells were incubated for 20 min before chase and during pulse with 10 μM gefitinib (Santa Cruz Biotechnology, 184475-35-2), 10 μM PP2 (Thermo-Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed and incubated at room temperature for 2 hours with the appropriate HRP-conjugated secondary antibodies as follows: mouse anti-rabbit (1/1000 dilution; Santa Cruz; cat #sc-2357) and mouse IgGκ BP (1/1000 dilution ...
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bioRxiv - Immunology 2019Quote: ... the membrane was re-blocked with 5% BSA in 1x TBST for 2 hour at room temperature before probing with a 1:200 dilution anti-vinculin 7F9 (Santa Cruz Biotechnology; sc-73614) in 5% BSA/1x TBST for 1 hour at room temperature followed by anti-mouse IgG HRP secondary ...
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bioRxiv - Genetics 2021Quote: ... 300 million #H5 clone mESCs were infected with a 1:10 dilution of virus-containing supernatant for 24 h in the presence of 2 μg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220) (Before infection ...
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bioRxiv - Plant Biology 2022Quote: ... It was then probed by an anti-GFP mouse monoclonal antibody (1:600, clone B-2; sc-9996, Santa Cruz Biotechnology, Dallas, Texas, USA). Anti-mouse IgG conjugated by peroxidase (A9044 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cells were blocked in 3% BSA in PBS with 0,2% Tween 20 and incubated overnight with anti-MYC (A-14, Santa Cruz Biotechnology, 2 ug/ml), anti-CENP-C (PD030 ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were removed from dishes and assessed by flow cytometry for construct expression and selected in puromycin 2 μg/mL (Santa Cruz Biotech, sc-108071B). Phoenix-Eco or Phoenix-Eco alpha-V beta-3 were transfected with PB-vector constructs as above ...
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bioRxiv - Immunology 2023Quote: ... Lungs were dissected at the stated time post tumor initiation and fixed for 24 hours in 2% paraformaldehyde (Santa Cruz Biotechnology Cat# sc-253236) at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... were used to produce HCT116 H2B-GFP ABAT knock down cells at a multiplicity of infection (MOI) of 5 and 2 mg/mL of puromycin (Santa Cruz Biotechnology, #sc-108071) was used to select for knocked down cells ...
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bioRxiv - Cell Biology 2024Quote: Cells in growth medium without penicillin and streptomycin were transfected with the hTRPV4 shRNA plasmid (1 and 2 µg; Santa Cruz sc-61726-SH) and Lipofectamine3000 in opti-MEM ...
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bioRxiv - Biophysics 2021Quote: ... The primary antibodies used for the immunostaining were mouse monoclonal antibodies (Arp3 [A-1], sc-48344; formin 2 [C-3], sc-376787; Santa Cruz Biotechnology, Dallas, Texas, USA). The primary antibodies were diluted 1:200 in 3% BSA and left in contact with the cells for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... The blots were blocked with 3% bovine serum albumin in TBS-T for 2 hours and incubated overnight with BAX (mouse monoclonal, Santa Cruz Biotechnology, Dallas, TX, USA) and Bcl-2 (mouse monoclonal ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing IgG to the SARS-CoV-2 nucleoprotein (NP) and with mouse anti-human GAPDH monoclonal antibody (G-9: Santa Cruz Biotechnology, Dallas, TX, USA). The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... A mixture of 0.5 µm Alexa Fluor 488 lysine labeled NSARS-CoV-2 and 1:100 Alexa Fluor 594 conjugated G3BP1 antibody (Santa Cruz Biotechnology, sc-365338 AF594) in cell permeabilization buffer was added to the cells and movies were recorded with 512 x 512 pixel resolution at 1000 Hz speed and 1 s per frame for about 2-3 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was incubated for 1h at room temperature in blocking buffer (5% nonfat milk in PBS pH 7.4 containing 0.1% Triton X-100 and 0.02% NaN3) and immunoblotted for 2 h at room temperature with antibodies raised against insulin (Santa Cruz Biotechnology Inc, Santa Cruz, CA), the porosome associated protein ATP2C1 and actin (Santa Cruz Biotechnology Inc ...
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bioRxiv - Molecular Biology 2022Quote: ... 1/10000) (Zhu et al. 2010), anti-Red1 (chicken, 1/3000) (Shinohara et al. 2008) and anti-α-tubulin (YL1/2, Santa Cruz Biotechnology, 1/3000). Tubulin signals were visualized using the LI-COR Bioscience system or alkaline phosphatase stain (BCIP-NBT solution kit ...
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies were used in the indicated dilutions: anti-α-tubulin (1:1000, B-5-1-2, Santa Cruz, USA, cat no sc-23948), anti-β-actin (1:10000 ...
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bioRxiv - Physiology 2021Quote: ... Kidney cortex cryosections were labeled with a polyclonal goat anti-aquaporin-2 (Aqp2) antibody (0.2 μg/ml, sc-9882; Santa Cruz Biotechnology, Santa Cruz CA, USA), and detected using a donkey anti-goat immunoglobulin G conjugated to Cyanine Cy3 (0.5 μg/ml ...
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bioRxiv - Biochemistry 2019Quote: ... The primary antibody used to detect UCP2 was 1:2000 dilution of anti-UCP1/2/3 raised in rabbit (Santa Cruz Biotechnology Inc. Dallas, Texas), and for AAC1 the primary antibody was 1:5000 dilution of rabbit IgG anti-AAC1 (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... On the day of the transfection two separate solutions were prepared: Solution A was made by adding 2 μg of plasmid DNA (Santa Cruz Biotechnology ®, sc-405646) into 130 μl of plasmid transfection medium (Santa Cruz Biotechnology ® ...
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bioRxiv - Developmental Biology 2022Quote: Primary antibodies used in this study are as follows: rabbit anti-CTP synthase 1/2 (y88) (1:1000, Santa Cruz Biotechnology, Cat. No. sc-134457) and mouse anti-HTS (1:1000 ...
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bioRxiv - Biophysics 2024Quote: ... TDP-43 aggregation levels were detected by GFP fluorescence signal and immunoblotting for GFP using α-GFP (B-2) primary antibody (Santa Cruz Biotechnology, sc-9996) with α-mouse IgG secondary antibody (MBL).
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bioRxiv - Immunology 2024Quote: ... Whole cell lysates of Th2 cells were prepared and immunoprecipitated with 2 µg of pulldown antibodies (Gata3, VDR, Gfi1) using protein G agarose beads (Santa Cruz Biotechnology, Dallas, TX, USA). Eluted conjugates were run on SDS-PAGE and probed with primary antibodies of partner proteins ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were subsequently incubated with primary antibodies against Pax7 (Developmental Studies Hybridoma Bank, 2 μg/mL) and MyoD (Santa Cruz Biotechnology, clone G-1, 1:200) diluted in 2.5% BSA and 2.5% GS in PBST ...
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bioRxiv - Immunology 2023Quote: Chromatin from flow-sorted small pre-B cells (2×106) from WT and Brwd1-/- mice were used for each ChIP experiment with anti-BRWD1 (E-15, Santa Cruz Biotechnology, sc-83517, lot J1508), anti-H3K4me1 (07-436 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2, sc-233X, Santa Cruz; POU2AF1, sc-955X, Santa Cruz; p300, A300-358A, Bethyl). ATAC-Seq was performed as previously described10 with nuclei isolated from 50,000 cells for each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% CO2.GIV knock-out (KO) cell lines were generated using Pooled guide RNA plasmids (commercially obtained from Santa Cruz Biotechnology; Cat# sc-402236-KO-2), as described earlier(67) ...